LOCUS       RC226352                8427 bp    DNA     circular  SYN 
DEFINITION  MCF2L (Myc-DDK-tagged)-Human MCF.2 cell line derived transforming sequence-like (MCF2L), transcript variant 1
ACCESSION   NM_001112732
VERSION     NM_001112732.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4584..4675 
                     /label="Myc-DDK"  
     misc_feature    4705..5290 
                     /label="PolyA signal"  
     misc_feature    4762..4781 
                     /label="Restriction Enzyme"  
     misc_feature    5566..6238 
                     /label="ColE1"  
     misc_feature    6668..7462 
                     /label="Kan/Neo"  
     misc_feature    7497..7799 
                     /label="SV40 ori"  
     misc_feature    7885..8362 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4566..4572 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4566 
                     /label="MCF2L"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGGGTG GAGAGCCCCG TGCCTGCAGC GCGCGCTTCC CTCACTGGGT 
     1081 CCTGCGTCCT TGGGCAGGCG ATGCCCCTGC GGGGAGGGGC TGGTCCATCC CCGGCCAGCC 
     1141 ACGGACCCAC GCATGGACCC AGCGACCCAC GGACCTGCTT ACCTGGGCGC GGCGCGGGTG 
     1201 GCATGCGGCC ACACGGAAGG GGCGCGCTGG GCTGCTGCGG CCTCTGCAGC TTCTACACCT 
     1261 GCCACGGGGC GGCCGGAGAT GAAATCATGC ACCAGGACAT CGTCCCGCTC TGTGCTGCCG 
     1321 ACATCCAGGA CCAGCTAAAG AAGCGCTTTG CTTACCTGTC CGGTGGGCGG GGGCAGGACG 
     1381 GAAGCCCGGT TATCACCTTC CCTGACTACC CGGCCTTCAG CGAGATTCCG GACAAGGAGT 
     1441 TCCAGAATGT CATGACCTAC CTCACCAGCA TCCCCAGCCT GCAGGACGCT GGCATCGGAT 
     1501 TCATCCTGGT GATAGACCGG CGACGGGACA AATGGACCTC CGTGAAGGCG TCCGTCCTGC 
     1561 GCATCGCAGC ATCTTTCCCG GCAAACCTGC AGCTCGTCCT CGTGCTTCGC CCGACGGGTT 
     1621 TTTTCCAAAG GACTCTCTCC GACATCGCTT TCAAATTCAA TAGAGATGAC TTTAAGATGA 
     1681 AGGTGCCGGT CATAATGCTG AGCTCCGTAC CAGACTTACA CGGTTACATC GATAAGTCGC 
     1741 AGCTGACCGA GGACCTGGGT GGGACCCTGG ACTACTGCCA CTCCCGGTGG CTGTGCCAGC 
     1801 GCACGGCCAT CGAAAGTTTC GCCCTCATGG TGAAGCAGAC GGCTCAGATG CTGCAGTCCT 
     1861 TCGGGACCGA GCTGGCTGAA ACAGAGCTGC CCAATGACGT CCAGTCGACA AGCTCAGTGC 
     1921 TGTGTGCGCA CACAGAGAAG AAGGACAAGG CGAAGGAGGA TTTGAGGCTG GCACTGAAAG 
     1981 AGGGGCACAG TGTCCTGGAG AGCCTCAGGG AGCTGCAGGC TGAGGGCTCA GAGCCCAGTG 
     2041 TGAACCAGGA CCAGCTTGAC AACCAGGCCA CCGTGCAGAG GCTCCTGGCC CAGCTGAACG 
     2101 AAACCGAGGC TGCCTTCGAT GAGTTCTGGG CAAAGCATCA GCAGAAACTG GAGCAGTGTC 
     2161 TGCAGCTCCG GCACTTTGAG CAGGGCTTCC GGGAGGTCAA AGCCATCTTG GACGCAGCGT 
     2221 CCCAGAAGAT AGCAACCTTC ACAGACATCG GCAACAGCCT GGCGCATGTG GAGCACCTGC 
     2281 TGAGGGACCT GGCCAGCTTC GAGGAGAAAT CAGGCGTGGC CGTGGAGAGG GCCCGGGCCC 
     2341 TGTCTCTGGA CGGCGAGCAG CTCATTGGGA ACAAGCACTA CGCGGTAGAC TCCATCCGCC 
     2401 CAAAGTGCCA GGAGCTCCGG CACCTCTGTG ACCAGTTCTC TGCGGAGATC GCAAGGAGGA 
     2461 GGGGGCTGCT CAGCAAGTCC CTGGAGCTGC ACCGCCGCCT GGAGACGTCC ATGAAGTGGT 
     2521 GTGATGAAGG GATTTACCTG CTGGCCTCAC AACCTGTGGA CAAGTGCCAG TCCCAGGACG 
     2581 GCGCGGAGGC TGCCCTCCAG GAAATCGAGA AGTTTTTGGA GACCGGTGCG GAAAATAAGA 
     2641 TCCAGGAGCT CAACGCGATT TACAAGGAAT ACGAATCCAT CCTCAACCAA GATCTCATGG 
     2701 AGCACGTGCG AAAGGTCTTC CAGAAGCAGG CAAGCATGGA GGAGGTGTTC CACCGCAGGC 
     2761 AGGCCAGCCT GAAGAAGCTG GCGGCCAGGC AGACGCGGCC CGTGCAGCCG GTGGCCCCCA 
     2821 GACCCGAGGC ACTGGCAAAG TCGCCCTGCC CCTCCCCAGG CATTCGGCGA GGCTCTGAGA 
     2881 ACTCCAGCTC CGAGGGCGGT GCGCTCCGGA GAGGGCCCTA CCGGAGGGCC AAGAGTGAGA 
     2941 TGAGTGAGAG CCGGCAGGGC CGCGGCTCAG CGGGGGAGGA GGAGGAAAGC CTGGCCATCC 
     3001 TGCGCAGGCA CGTGATGAGC GAGCTCCTGG ACACAGAACG GGCCTACGTG GAGGAGCTGC 
     3061 TGTGCGTCCT GGAGGGCTAC GCCGCGGAGA TGGATAACCC ACTGATGGCT CACCTCCTGT 
     3121 CAACAGGCCT TCACAACAAG AAGGATGTTT TGTTTGGAAA CATGGAGGAA ATCTATCACT 
     3181 TCCACAACAG GATATTCCTC AGGGAGCTGG AAAACTACAC TGACTGCCCA GAACTGGTTG 
     3241 GAAGATGCTT TCTGGAGAGG ATGGAAGATT TCCAGATCTA TGAGAAGTAC TGTCAGAACA 
     3301 AGCCCCGCTC TGAGAGCCTG TGGAGACAGT GCTCCGACTG CCCGTTTTTC CAGGAATGCC 
     3361 AGAGAAAGCT GGACCACAAG CTGAGCCTGG ACTCCTACCT GCTGAAGCCA GTGCAGAGGA 
     3421 TCACCAAGTA CCAGCTGCTG CTCAAGGAAA TGCTGAAATA CAGCAGGAAC TGCGAGGGGG 
     3481 CTGAGGACCT GCAGGAGGCG CTGAGCTCCA TCCTGGGCAT CCTGAAGGCC GTGAACGACT 
     3541 CCATGCACCT CATCGCTATC ACCGGCTATG ACGGGAATCT CGGCGACCTG GGCAAGCTGC 
     3601 TGATGCAGGG CTCGTTCAGC GTCTGGACCG ACCACAAGAG GGGCCACACC AAGGTGAAGG 
     3661 AGCTGGCCAG GTTCAAGCCC ATGCAGCGGC ACCTGTTCCT GCACGAGAAG GCAGTGCTCT 
     3721 TCTGCAAGAA GAGGGAGGAG AATGGGGAGG GGTATGAGAA AGCTCCCTCC TACAGCTACA 
     3781 AGCAGTCCTT AAACATGGCT GCCGTTGGCA TTACGGAGAA CGTGAAGGGA GATGCTAAGA 
     3841 AGTTCGAGAT CTGGTACAAC GCGCGCGAGG AGGTCTACAT CGTCCAGGCG CCAACTCCTG 
     3901 AGATTAAAGC CGCGTGGGTG AATGAAATTC GGAAAGTGCT GACCAGCCAG CTGCAGGCTT 
     3961 GTAGAGAAGC CAGCCAGCAC CGGGCGCTGG AGCAGTCACA GAGCCTGCCC CTGCCGGCCC 
     4021 CGACCAGCAC CAGTCCCTCA AGAGGAAACT CAAGGAACAT CAAGAAGCTG GAAGAAAGGA 
     4081 AAACAGACCC CCTAAGCCTG GAGGGATACG TCAGCTCAGC GCCACTGACA AAGCCCCCCG 
     4141 AAAAGGGCAA AGGTTGGAGC AAAACGTCCC ACTCACTGGA GGCACCTGAG GACGACGGGG 
     4201 GCTGGTCAAG TGCAGAGGAG CAGATTAACT CGTCCGACGC AGAGGAGGAC GGCGGGTTGG 
     4261 GCCCCAAGAA GCTGGTTCCA GGTAAATACA CGGTCGTGGC GGACCACGAG AAGGGAGGCC 
     4321 CCGATGCGCT GCGCGTGAGG AGCGGGGACG TGGTGGAGCT GGTGCAGGAG GGCGACGAGG 
     4381 GCCTCTGGTA CGTCAGGGAC CCGACCACTG GCAAGGAGGG CTGGGTGCCG GCCAGCAGCC 
     4441 TGTCCGTCCG GCTCGGCCCG TCCGGCTCGG CCCAGTGCCT GAGCAGCTCA GAGTCGAGCC 
     4501 CGGGGTCGGC CGTGCTGAGC AACTCGTCCA GCTGCAGCGA GGGCGGCCAG GCCCCCTTCT 
     4561 CCGACCTGCA GGGGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4621 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4681 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4741 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4801 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4861 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4921 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4981 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5041 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5101 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5161 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5221 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5281 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5341 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5401 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6001 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6061 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6121 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6181 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6241 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6301 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6361 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6421 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6481 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6541 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6601 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6661 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6721 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6781 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6841 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6901 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6961 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7021 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7081 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7141 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7201 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7261 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7321 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7381 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7441 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7501 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7561 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7621 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7861 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7921 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7981 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8041 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8101 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8161 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8221 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8281 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8341 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8401 ATTTAACAAA AATTTAACGC GAATTTT
//