LOCUS       RC226306                8001 bp    DNA     circular  SYN 
DEFINITION  NLRP7 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 7 (NLRP7), transcript variant 3
ACCESSION   NM_001127255
VERSION     NM_001127255.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4156..4247 
                     /label="Myc-DDK"  
     misc_feature    4277..4862 
                     /label="PolyA signal"  
     misc_feature    4334..4353 
                     /label="Restriction Enzyme"  
     misc_feature    5138..5810 
                     /label="ColE1"  
     misc_feature    6240..7034 
                     /label="Kan/Neo"  
     misc_feature    7069..7371 
                     /label="SV40 ori"  
     misc_feature    7457..7934 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4131..4138 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4131 
                     /label="NLRP7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACATCGCCC CAGCTAGAGT GGACTCTGCA GACCCTTCTG GAGCAGCTGA 
     1081 ACGAGGATGA ATTAAAGAGT TTCAAATCCC TTTTATGGGC TTTTCCCCTC GAAGACGTGC 
     1141 TACAGAAGAC CCCATGGTCT GAGGTGGAAG AGGCTGATGG CAAGAAACTG GCAGAAATTC 
     1201 TGGTCAACAC CTCCTCAGAA AATTGGATAA GGAATGCGAC TGTGAACATC TTGGAAGAGA 
     1261 TGAATCTCAC GGAATTGTGT AAGATGGCAA AGGCTGAGAT GATGGAGGAC GGACAGGTGC 
     1321 AAGAAATAGA TAATCCTGAG CTGGGAGATG CAGAAGAAGA CTCGGAGTTA GCAAAGCCAG 
     1381 GTGAAAAGGA AGGATGGAGA AATTCAATGG AGAAACAATC TTTGGTCTGG AAGAACACCT 
     1441 TTTGGCAAGG AGACATTGAC AATTTCCATG ACGACGTCAC TCTGAGAAAC CAACGGTTCA 
     1501 TTCCATTCTT GAATCCCAGA ACACCCAGGA AGCTAACACC TTACACGGTG GTGCTGCACG 
     1561 GCCCCGCAGG CGTGGGGAAA ACCACGCTGG CCAAAAAGTG TATGCTGGAC TGGACAGACT 
     1621 GCAACCTCAG CCCGACGCTC AGATACGCGT TCTACCTCAG CTGCAAGGAG CTCAGCCGCA 
     1681 TGGGCCCCTG CAGTTTTGCA GAGCTGATCT CCAAAGACTG GCCTGAATTG CAGGATGACA 
     1741 TTCCAAGCAT CCTAGCCCAA GCACAGAGAA TCCTGTTCGT GGTCGATGGC CTTGATGAGC 
     1801 TGAAAGTCCC ACCTGGGGCG CTGATCCAGG ACATCTGCGG GGACTGGGAG AAGAAGAAGC 
     1861 CGGTGCCCGT CCTCCTGGGG AGTTTGCTGA AGAGGAAGAT GTTACCCAGG GCAGCCTTGC 
     1921 TGGTCACCAC GCGGCCCAGG GCACTGAGGG ACCTCCGGAT CCTGGCGCAG CAGCCGATCT 
     1981 ACGTAAGGGT GGAGGGCTTC CTGGAGGAGG ACAGGAGGGC CTATTTCCTG AGACACTTTG 
     2041 GAGACGAGGA CCAAGCCATG CGTGCCTTTG AGCTAATGAG GAGCAACGCG GCCCTGTTCC 
     2101 AGCTGGGCTC GGCCCCCGCG GTGTGCTGGA TTGTGTGCAC GACTCTGAAG CTGCAGATGG 
     2161 AGAAGGGGGA GGACCCGGTC CCCACCTGCC TCACCCGCAC GGGGCTGTTC CTGCGTTTCC 
     2221 TCTGCAGCCG GTTCCCGCAG GGCGCACAGC TGCGGGGCGC GCTGCGGACG CTGAGCCTCC 
     2281 TGGCCGCGCA GGGCCTGTGG GCGCAGATGT CCGTGTTCCA CCGAGAGGAC CTGGAAAGGC 
     2341 TCGGGGTGCA GGAGTCCGAC CTCCGTCTGT TCCTGGACGG AGACATCCTC CGCCAGGACA 
     2401 GAGTCTCCAA AGGCTGCTAC TCCTTCATCC ACCTCAGCTT CCAGCAGTTT CTCACTGCCC 
     2461 TGTTCTACAC CCTGGAGAAG GAGGAGGGGG AGGACAGGGA CGGCCACGCC TGGGACATCG 
     2521 GGGACGTACA GAAGCTGCTT TCCGGAGAAG AAAGACTCAA GAACCCCGAC CTGATTCAAG 
     2581 TAGGACACTT CTTATTCGGC CTCGCTAACG AGAAGAGAGC CAAGGAGTTG GAGGCCACTT 
     2641 TTGGCTGCCG GATGTCACCG GACATCAAAC AGGAATTGCT GCAATGCAAA GCACATCTTC 
     2701 ATGCAAATAA GCCCTTATCC GTGACCGACC TGAAGGAGGT CTTGGGCTGC CTGTATGAGT 
     2761 CTCAGGAGGA GGAGCTGGCG AAGGTGGTGG TGGCCCCGTT CAAGGAAATT TCTATTCACC 
     2821 TGACAAATAC TTCTGAAGTG ATGCATTGTT CCTTCAGCCT GAAGCATTGT CAAGACTTGC 
     2881 AGAAACTCTC ACTGCAGGTA GCAAAGGGGG TGTTCCTGGA GAATTACATG GATTTTGAAC 
     2941 TGGACATTGA ATTTGAAAGG TGCACTTACC TAACCATTCC GAACTGGGCT CGGCAGGATC 
     3001 TTCGCTCTCT TCGCCTCTGG ACAGATTTCT GCTCTCTCTT CAGCTCAAAC AGCAACCTCA 
     3061 AGTTTCTGGA AGTGAAACAA AGCTTCCTGA GTGACTCTTC TGTGCGGATT CTTTGTGACC 
     3121 ACGTAACCCG TAGCACCTGT CATCTGCAGA AAGTGGAGAT TAAAAACGTC ACCCCTGACA 
     3181 CCGCGTACCG GGACTTCTGT CTTGCTTTCA TTGGGAAGAA GACCCTCACG CACCTGACCC 
     3241 TGGCAGGGCA CATCGAGTGG GAACGCACGA TGATGCTGAT GCTGTGTGAC CTGCTCAGAA 
     3301 ATCATAAATG CAACCTGCAG TACCTGAGGT TGGGAGGTCA CTGTGCCACC CCGGAGCAGT 
     3361 GGGCTGAATT CTTCTATGTC CTCAAAGCCA ACCAGTCCCT GAAGCACCTG CGTCTCTCAG 
     3421 CCAATGTGCT CCTGGATGAG GGTGCCATGT TGCTGTACAA GACCATGACA CGCCCAAAAC 
     3481 ACTTCCTGCA GATGTTGTCG TTGGAAAACT GTCGTCTTAC AGAAGCCAGT TGCAAGGACC 
     3541 TTGCTGCTGT CTTGGTTGTC AGCAAGAAGC TGACACACCT GTGCTTGGCC AAGAACCCCA 
     3601 TTGGGGATAC AGGGGTGAAG TTTCTGTGTG AGGGCTTGAG TTACCCTGAT TGTAAACTGC 
     3661 AGACCTTGGT GTTACAGCAA TGCAGCATAA CCAAGCTTGG CTGTAGATAC CTCTCAGAGG 
     3721 CGCTCCAAGA AGCCTGCAGC CTCACAAACC TGGACTTGAG TATCAACCAG ATAGCTCGTG 
     3781 GATTGTGGAT TCTCTGTCAG GCGTTAGAGA ATCCAAACTG TAACCTAAAA CACCTACGCC 
     3841 TCTGGAGCTG CTCCCTCATG CCTTTCTATT GTCAGCATCT TGGATCTGCT CTCCTCAGCA 
     3901 ATCAGAAGCT TGAAACTCTG GACCTGGGCC AGAATCATTT GTGGAAGAGT GGCATAATTA 
     3961 AGCTCTTTGG GGTTCTAAGA CAAAGAACTG GATCCTTGAA GATACTCAGG TTGAAGACCT 
     4021 ATGAAACTAA TTTGGAAATC AAGAAGCTGT TGGAGGAAGT GAAAGAAAAG AATCCCAAGC 
     4081 TGACTATTGA TTGCAATGCT TCCGGGGCAA CGGCACCTCC GTGCTGTGAC TTTTTTTGCA 
     4141 GCGGACCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4201 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4261 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4321 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4381 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4441 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4501 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4561 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4621 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4681 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4741 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4801 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4861 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4921 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4981 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5041 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5101 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5161 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5221 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5281 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5341 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5401 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5461 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5521 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5581 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5641 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5701 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5761 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5821 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5881 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5941 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6001 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6061 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6121 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6181 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6241 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6301 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6361 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6421 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6481 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6541 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6601 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6661 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6721 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6781 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6841 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6901 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6961 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7021 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7081 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7141 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7201 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7261 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7321 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7381 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7441 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7501 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7561 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7621 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7681 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7741 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7801 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7861 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7921 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7981 CAAAAATTTA ACGCGAATTT T
//