LOCUS       RC226286L1                9312 bp    DNA     circular  SYN 
DEFINITION  NLRP3 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 4
ACCESSION   NM_001127461
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7603..7783 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8511..9170 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7942..8510 
                     /label="ColE1"  
     misc_feature    6478..6498 
                     /label="LR50 primer"  
     misc_feature    6275..6346 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6487..7078 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6257..6263 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6257 
                     /label="NLRP3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAAGATGGCA AGCACCCGCT GCAAGCTGGC 
     3361 CAGGTACCTG GAGGACCTGG AGGATGTGGA CTTGAAGAAA TTTAAGATGC ACTTAGAGGA 
     3421 CTATCCTCCC CAGAAGGGCT GCATCCCCCT CCCGAGGGGT CAGACAGAGA AGGCAGACCA 
     3481 TGTGGATCTA GCCACGCTAA TGATCGACTT CAATGGGGAG GAGAAGGCGT GGGCCATGGC 
     3541 CGTGTGGATC TTCGCTGCGA TCAACAGGAG AGACCTTTAT GAGAAAGCAA AAAGAGATGA 
     3601 GCCGAAGTGG GGTTCAGATA ATGCACGTGT TTCGAATCCC ACTGTGATAT GCCAGGAAGA 
     3661 CAGCATTGAA GAGGAGTGGA TGGGTTTACT GGAGTACCTT TCGAGAATCT CTATTTGTAA 
     3721 AATGAAGAAA GATTACCGTA AGAAGTACAG AAAGTACGTG AGAAGCAGAT TCCAGTGCAT 
     3781 TGAAGACAGG AATGCCCGTC TGGGTGAGAG TGTGAGCCTC AACAAACGCT ACACACGACT 
     3841 GCGTCTCATC AAGGAGCACC GGAGCCAGCA GGAGAGGGAG CAGGAGCTTC TGGCCATCGG 
     3901 CAAGACCAAG ACGTGTGAGA GCCCCGTGAG TCCCATTAAG ATGGAGTTGC TGTTTGACCC 
     3961 CGATGATGAG CATTCTGAGC CTGTGCACAC CGTGGTGTTC CAGGGGGCGG CAGGGATTGG 
     4021 GAAAACAATC CTGGCCAGGA AGATGATGTT GGACTGGGCG TCGGGGACAC TCTACCAAGA 
     4081 CAGGTTTGAC TATCTGTTCT ATATCCACTG TCGAGAGGTG AGCCTTGTGA CACAGAGGAG 
     4141 CCTGGGGGAC CTGATCATGA GCTGCTGCCC CGACCCAAAC CCACCCATCC ACAAGATCGT 
     4201 GAGAAAACCC TCCAGAATCC TCTTCCTCAT GGACGGCTTC GATGAGCTGC AAGGTGCCTT 
     4261 TGACGAGCAC ATAGGACCGC TCTGCACTGA CTGGCAGAAG GCCGAGCGGG GAGACATTCT 
     4321 CCTGAGCAGC CTCATCAGAA AGAAGCTGCT TCCCGAGGCC TCTCTGCTCA TCACCACGAG 
     4381 ACCTGTGGCC CTGGAGAAAC TGCAGCACTT GCTGGACCAT CCTCGGCATG TGGAGATCCT 
     4441 GGGTTTCTCC GAGGCCAAAA GGAAAGAGTA CTTCTTCAAG TACTTCTCTG ATGAGGCCCA 
     4501 AGCCAGGGCA GCCTTCAGTC TGATTCAGGA GAACGAGGTC CTCTTCACCA TGTGCTTCAT 
     4561 CCCCCTGGTC TGCTGGATCG TGTGCACTGG ACTGAAACAG CAGATGGAGA GTGGCAAGAG 
     4621 CCTTGCCCAG ACATCCAAGA CCACCACCGC GGTGTACGTC TTCTTCCTTT CCAGTTTGCT 
     4681 GCAGCCCCGG GGAGGGAGCC AGGAGCACGG CCTCTGCGCC CACCTCTGGG GGCTCTGCTC 
     4741 TTTGGCTGCA GATGGAATCT GGAACCAGAA AATCCTGTTT GAGGAGTCCG ACCTCAGGAA 
     4801 TCATGGACTG CAGAAGGCGG ATGTGTCTGC TTTCCTGAGG ATGAACCTGT TCCAAAAGGA 
     4861 AGTGGACTGC GAGAAGTTCT ACAGCTTCAT CCACATGACT TTCCAGGAGT TCTTTGCCGC 
     4921 CATGTACTAC CTGCTGGAAG AGGAAAAGGA AGGAAGGACG AACGTTCCAG GGAGTCGTTT 
     4981 GAAGCTTCCC AGCCGAGACG TGACAGTCCT TCTGGAAAAC TATGGCAAAT TCGAAAAGGG 
     5041 GTATTTGATT TTTGTTGTAC GTTTCCTCTT TGGCCTGGTA AACCAGGAGA GGACCTCCTA 
     5101 CTTGGAGAAG AAATTAAGTT GCAAGATCTC TCAGCAAATC AGGCTGGAGC TGCTGAAATG 
     5161 GATTGAAGTG AAAGCCAAAG CTAAAAAGCT GCAGATCCAG CCCAGCCAGC TGGAATTGTT 
     5221 CTACTGTTTG TACGAGATGC AGGAGGAGGA CTTCGTGCAA AGGGCCATGG ACTATTTCCC 
     5281 CAAGATTGAG ATCAATCTCT CCACCAGAAT GGACCACATG GTTTCTTCCT TTTGCATTGA 
     5341 GAACTGTCAT CGGGTGGAGT CACTGTCCCT GGGGTTTCTC CATAACATGC CCAAGGAGGA 
     5401 AGAGGAGGAG GAAAAGGAAG GCCGACACCT TGATATGGTG CAGTGTGTCC TCCCAAGCTC 
     5461 CTCTCATGCT GCCTGTTCTC ATGGATTGGT GAACAGCCAC CTCACTTCCA GTTTTTGCCG 
     5521 GGGCCTCTTT TCAGTTCTGA GCACCAGCCA GAGTCTAACT GAATTGGACC TCAGTGACAA 
     5581 TTCTCTGGGG GACCCAGGGA TGAGAGTGTT GTGTGAAACG CTCCAGCATC CTGGCTGTAA 
     5641 CATTCGGAGA TTGTGGTTGG GGCGCTGTGG CCTCTCGCAT GAGTGCTGCT TCGACATCTC 
     5701 CTTGGTCCTC AGCAGCAACC AGAAGCTGGT GGAGCTGGAC CTGAGTGACA ACGCCCTCGG 
     5761 TGACTTCGGA ATCAGACTTC TGTGTGTGGG ACTGAAGCAC CTGTTGTGCA ATCTGAAGAA 
     5821 GCTCTGGTTG GTGAATTCTG GCCTTACGTC AGTCTGTTGT TCAGCTTTGT CCTCGGTACT 
     5881 CAGCACTAAT CAGAATCTCA CGCACCTTTA CCTGCGAGGC AACACTCTCG GAGACAAGGG 
     5941 GATCAAACTA CTCTGTGAGG GACTCTTGCA CCCCGACTGC AAGCTTCAGG TGTTGGAATT 
     6001 AGACAACTGC AACCTCACGT CACACTGCTG CTGGGATCTT TCCACACTTC TGACCTCCAG 
     6061 CCAGAGCCTG CGAAAGCTGA GCCTGGGCAA CAATGACCTG GGCGACCTGG GGGTCATGAT 
     6121 GTTCTGTGAA GTGCTGAAAC AGCAGAGCTG CCTCCTGCAG AACCTGGGGT TGTCTGAAAT 
     6181 GTATTTCAAT TATGAGACAA AAAGTGCGTT AGAAACACTT CAAGAAGAAA AGCCTGAGCT 
     6241 GACCGTCGTC TTTGAGCCTT CTTGGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC 
     6301 AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA 
     6361 AACGGCCGGC CGCGGTCTGT ACAAGTAGGA TTCGTCGAGG GACCTAATAA CTTCGTATAG 
     6421 CATACATTAT ACGAAGTTAT ACATGTTTAA GGGTTCCGGT TCCACTAGGT ACAATTCGAT 
     6481 ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT 
     6541 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT 
     6601 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT 
     6661 CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT 
     6721 GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC 
     6781 GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG 
     6841 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC 
     6901 TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC 
     6961 GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG 
     7021 CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC 
     7081 CCGCATCGAT ACCGTCGACC TCGATCGAGA CCTAGAAAAA CATGGAGCAA TCACAAGTAG 
     7141 CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA GCACAAGAGG AGGAGGAGGT 
     7201 GGGTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA 
     7261 TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG 
     7321 ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC TACTTCCCTG ATTGGCAGAA 
     7381 CTACACACCA GGGCCAGGGA TCAGATATCC ACTGACCTTT GGATGGTGCT ACAAGCTAGT 
     7441 ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC CAATGAAGGA GAGAACACCC GCTTGTTACA 
     7501 CCCTGTGAGC CTGCATGGGA TGGATGACCC GGAGAGAGAA GTATTAGAGT GGAGGTTTGA 
     7561 CAGCCGCCTA GCATTTCATC ACATGGCCCG AGAGCTGCAT CCGGACTGTA CTGGGTCTCT 
     7621 CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA 
     7681 GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC 
     7741 TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGCATGTG 
     7801 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     7861 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     7921 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     7981 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     8041 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     8101 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     8161 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     8221 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     8281 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     8341 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     8401 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     8461 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAT 
     8521 TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA 
     8581 TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG 
     8641 CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC 
     8701 ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC 
     8761 TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA 
     8821 TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT 
     8881 TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA 
     8941 CCGTCTTTCA TTGCCATACG GAACTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA 
     9001 ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 
     9061 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT 
     9121 TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATA 
     9181 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     9241 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTCC CGCGCACATT TCCCCGAAAA 
     9301 GTGCCACCTG AC
//