LOCUS       RC226257                7617 bp    DNA     circular  SYN 
DEFINITION  SLFN14 (Myc-DDK-tagged)-Human schlafen family member 14 (SLFN14)
ACCESSION   NM_001129820
VERSION     NM_001129820.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3774..3865 
                     /label="Myc-DDK"  
     misc_feature    3895..4480 
                     /label="PolyA signal"  
     misc_feature    3952..3971 
                     /label="Restriction Enzyme"  
     misc_feature    4756..5428 
                     /label="ColE1"  
     misc_feature    5858..6652 
                     /label="Kan/Neo"  
     misc_feature    6687..6989 
                     /label="SV40 ori"  
     misc_feature    7075..7552 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3756..3762 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3756 
                     /label="SLFN14"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGTCTC AAGACTGATA CTGAAATGCC GTATCCTGAG GTAATAGTAG 
     1081 ATGTGGGCAG AGTGATTTTT GGAGAAGAAA ACAGGAAGAA GATGACCAAC AGCTGTTTGA 
     1141 AAAGATCTGA GAATTCTAGA ATTATCCGGG CTATATGTGC ACTGTTAAAT TCTGGAGGTG 
     1201 GTGTGATCAA AGCAGAGATT GATGATAAAA CCTATAGTTA CCAATGCCAT GGGCTGGGAC 
     1261 AGGATTTGGA AACTTCTTTT CAAAAGCTCC TTCCTTCAGG TTCACAGAAA TACCTTGACT 
     1321 ACATGCAGCA GGGGCACAAT CTCCTGATTT TTGTGAAGTC ATGGAGCCCA GATGTTTTCA 
     1381 GCCTTCCACT AAGGATTTGC AGCTTGCGCT CCAATTTGTA TCGGAGAGAT GTGACTTCTG 
     1441 CTATCAACTT GAGTGCTAGC AGTGCCCTGG AGCTTCTCAG AGAGAAGGGG TTTAGAGCCC 
     1501 AAAGAGGAAG ACCAAGGGTG AAGAAGTTGC ATCCTCAGCA GGTTCTCAAT AGATGCATTC 
     1561 AGGAAGAGGA AGATATGAGG ATATTGGCCT CAGAATTTTT TAAAAAGGAC AAACTCATGT 
     1621 ATAAGGAGAA ACTCAACTTT ACTGAGTCAA CACATGTTGA ATTTAAAAGG TTCACCACCA 
     1681 AAAAAGTCAT ACCTCGGATT AAGGAAATGC TGCCTCATTA TGTTTCTGCA TTTGCCAACA 
     1741 CTCAAGGGGG ATATGTCCTC ATTGGGGTGG ATGATAAGAG CAAAGAAGTG GTTGGATGTA 
     1801 AGTGGGAAAA AGTGAATCCT GACTTACTAA AAAAAGAAAT CGAAAACTGC ATAGAAAAAT 
     1861 TGCCTACATT CCACTTCTGC TGTGAGAAGC CAAAGGTAAA TTTCACTACA AAAATCCTGA 
     1921 ATGTGTACCA AAAAGATGTC CTGGATGGTT ATGTCTGTGT GATTCAAGTG GAGCCCTTCT 
     1981 GTTGCGTGGT GTTTGCAGAG GCCCCAGATT CCTGGATCAT GAAAGACAAT TCTGTCACAC 
     2041 GGCTGACAGC TGAGCAGTGG GTGGTCATGA TGCTGGATAC TCAGTCAGCT CCTCCCAGTT 
     2101 TGGTCACAGA CTACAACTCT TGCCTGATTT CATCAGCTTC ATCTGCACGA AAAAGTCCCG 
     2161 GATATCCCAT AAAAGTCCAC AAATTTAAGG AGGCTCTGCA ACGACATTTG TTTCCAGTGA 
     2221 CACAGGAAGA GGTACAATTT AAACCAGAAT CCCTCTGTAA GAAGCTGTTC TCAGATCATA 
     2281 AAGAACTGGA GGGTTTAATG AAGACCCTGA TACATCCTTG TTCTCAGGGG ATTGTGATAT 
     2341 TTTCTAGAAG CTGGGCTGGT GATGTTGGCT TCAGGAAAGA ACAGAATGTC CTGTGTGATG 
     2401 CTCTCCTGAT AGCAGTTAAC AGCCCCGTGG TACTCTATAC AATCTTAATA GACCCCAATT 
     2461 GGCCTGGAGG ACTTGAATAT GCCCGAAACA CAGCTCATCA GTTAAAGCAG AAACTGCAAA 
     2521 CTGTTGGTGG TTACACAGGG AAAGTGTGCA TCATTCCAAG GCTGATACAC CTGAGCAGCA 
     2581 CACAGAGTAG ACCTGGTGAG ATCCCCCTGC GTTACCCCAG GTCCTACAGG CTTGCTGATG 
     2641 AGGAGGAAAT GGAAGACTTG CTGCAGGCTC TGGTGGTGGT CTCCCTGTCC TCCAGATCTC 
     2701 TTCTGAGTGA CCAGATGGGC TGTGAATTTT TCAACTTGCT CATAATGGAG CAGAGCCAGT 
     2761 TGCTTTCTGA GAGTCTTCAG AAGACACGTG AATTATTCAT CTACTGCTTT CCAGGAGTCA 
     2821 GGAAGACAGC CCTAGCCATA AAGATCATGG AGAAAATTAA GGACTTGTTT CACTGCAAAC 
     2881 CAAAAGAGAT CCTCTATGTT TGTGAAAGCG ACTCCCTAAA GGATTTTGTG ACCCAACAAA 
     2941 CCACCTGCCA AGCTGTGACC AGGAAAACTT TCATGCAAGG GGAGTTTCTA AAGATTAAAC 
     3001 ACATAGTGAT GGATGAGACT GAGAATTTCT GCAGCAAATA TGGCAATTGG TACATGAAGG 
     3061 CTAAGAACAT CACCCATCCA AAGGCGAAGG GGACTGGAAG TGAAAACCTT CACCATGGGA 
     3121 TTCTCTGGCT TTTTCTTGAC CCTTTTCAAA TCCATCACGC AGATGTCAAT GGCCTTCCCC 
     3181 CTCCATCTGC TCAGTTTCCT CGAAAAACAA TCACCAGTGG GATCCACTGT GCTCTGGAAA 
     3241 TAGCGAAGGT TATGAAAGAA GAAATGAAGA GGATCAAAGA AAATCCTCCC TCCAACATGT 
     3301 CTCCAGACAC ATTAGCATTG TTCAGCGAGA CTGCCTATGA GGAAGCAACG TGTGCCCAGG 
     3361 CTCTGCCTGG GGTGTGTGAG ACAAAGACTA ATCTGACAAC AGAACAAATA GCTAACTATG 
     3421 TGGCAAGAAA ATGTCACAGC CTGTTCCAGT GTGGCTATCT GCCCAAAGAT ATAGCAATTC 
     3481 TGTGCAGGAG AGGGGAGGAC AGAGGACGCT ATAGGCTTGC ACTACTCAAA GCAATGGAAT 
     3541 TAATTGAGAC CCACAGACCA TCAGAGGTTG TGTTTAGCCC GGCCACCGGT GTTTGGGGAA 
     3601 GTCACATTGT TTTAGACAGT ATTCAGCAAT TTTCAGGCCT GGAGAGGACT GTCGTGTTTG 
     3661 GGCTTAGTCC AGAATGTGAC CAGTCAGAGG AATTTCATAA GCTCTGCTTT GCTTCAAGAG 
     3721 CCATTAAACA CCTCTACCTG CTTTATGAAA AGAGGGCAGC CTACACGCGT ACGCGGCCGC 
     3781 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3841 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3901 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3961 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4021 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4081 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4141 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4201 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4261 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4321 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4381 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4441 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4501 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4561 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4621 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4681 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4741 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4801 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4861 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4921 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4981 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5041 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5101 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5161 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5221 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5281 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5341 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5401 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5461 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5521 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5581 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5641 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5701 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5761 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5821 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5881 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5941 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6001 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6061 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6121 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6181 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6241 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6301 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6361 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6421 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6481 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6541 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6601 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6661 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6721 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6781 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6841 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6901 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6961 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7021 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7081 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7141 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7201 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7261 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7321 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7381 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7441 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7501 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7561 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//