LOCUS       RC226253L1                9093 bp    DNA     circular  SYN 
DEFINITION  GRIA1 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, AMPA 1 (GRIA1), transcript variant 2
ACCESSION   NM_001114183
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7384..7564 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8292..8951 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7723..8291 
                     /label="ColE1"  
     misc_feature    6259..6279 
                     /label="LR50 primer"  
     misc_feature    6056..6127 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6268..6859 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6038..6044 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6038 
                     /label="GRIA1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCAGCACATT TTTGCCTTCT TCTGCACCGG 
     3361 TTTCCTAGGC GCGGTAGTAG GTGCCAATTT CCCCAACAAT ATCCAGATCG GGGGATTATT 
     3421 TCCAAACCAG CAGTCACAGG AACATGCTGC TTTTAGATTT GCTTTGTCGC AACTCACAGA 
     3481 GCCCCCGAAG CTGCTCCCCC AGATTGATAT TGTGAACATC AGCGACAGCT TTGAGATGAC 
     3541 CTATAGATTC TGTTCCCAGT TCTCCAAAGG AGTCTATGCC ATCTTTGGGT TTTATGAACG 
     3601 TAGGACTGTC AACATGCTGA CCTCCTTTTG TGGGGCCCTC CACGTCTGCT TCATTACGCC 
     3661 GAGCTTTCCC GTTGATACAT CCAATCAGTT TGTCCTTCAG CTGCGCCCTG AACTGCAGGA 
     3721 TGCCCTCATC AGCATCATTG ACCATTACAA GTGGCAGAAA TTTGTCTACA TTTATGATGC 
     3781 CGACCGGGGC TTATCCGTCC TGCAGAAAGT CCTGGATACA GCTGCTGAGA AGAACTGGCA 
     3841 GGTGACAGCA GTCAACATTT TGACAACCAC AGAGGAGGGA TACCGGATGC TCTTTCAGGA 
     3901 CCTGGAGAAG AAAAAGGAGC GGCTGGTGGT GGTGGACTGT GAATCAGAAC GCCTCAATGC 
     3961 TATCTTGGGC CAGATTATAA AGCTAGAGAA GAATGGCATC GGCTACCACT ACATTCTTGC 
     4021 AAATCTGGGC TTCATGGACA TTGACTTAAA CAAATTCAAG GAGAGTGGCG CCAATGTGAC 
     4081 AGGTTTCCAG CTGGTGAACT ACACAGACAC TATTCCGGCC AAGATCATGC AGCAGTGGAA 
     4141 GAATAGTGAT GCTCGAGACC ACACACGGGT GGACTGGAAG AGACCCAAGT ACACCTCTGC 
     4201 GCTCACCTAC GATGGGGTGA AGGTGATGGC TGAGGCTTTC CAGAGCCTGC GGAGGCAGAG 
     4261 AATTGATATA TCTCGCCGGG GGAATGCTGG GGATTGTCTG GCTAACCCAG CTGTTCCCTG 
     4321 GGGCCAAGGG ATCGACATCC AGAGAGCTCT GCAGCAGGTG CGATTTGAAG GTTTAACAGG 
     4381 AAACGTGCAG TTTAATGAGA AAGGACGCCG GACCAACTAC ACGCTCCACG TGATTGAAAT 
     4441 GAAACATGAC GGCATCCGAA AGATTGGTTA CTGGAATGAA GATGATAAGT TTGTCCCTGC 
     4501 AGCCACCGAT GCCCAAGCTG GGGGCGATAA TTCAAGTGTT CAGAACAGAA CATACATCGT 
     4561 CACAACAATC CTAGAAGATC CTTATGTGAT GCTCAAGAAG AACGCCAATC AGTTTGAGGG 
     4621 CAATGACCGT TACGAGGGCT ACTGTGTAGA GCTGGCGGCA GAGATTGCCA AGCACGTGGG 
     4681 CTACTCCTAC CGTCTGGAGA TTGTCAGTGA TGGAAAATAC GGAGCCCGAG ACCCTGACAC 
     4741 GAAGGCCTGG AATGGCATGG TGGGAGAGCT GGTCTATGGA AGAGCAGATG TGGCTGTGGC 
     4801 TCCCTTAACT ATCACTTTGG TCCGGGAAGA AGTTATAGAT TTCTCCAAAC CATTTATGAG 
     4861 TTTGGGGATC TCCATCATGA TTAAAAAACC ACAGAAATCC AAGCCGGGTG TCTTCTCCTT 
     4921 CCTTGATCCT TTGGCTTATG AGATTTGGAT GTGCATTGTT TTTGCCTACA TTGGAGTGAG 
     4981 TGTTGTCCTC TTCCTGGTCA GCCGCTTCAG TCCCTATGAA TGGCACAGTG AAGAGTTTGA 
     5041 GGAAGGACGG GACCAGACAA CCAGTGACCA GTCCAATGAG TTTGGGATAT TCAACAGTTT 
     5101 GTGGTTCTCC CTGGGAGCCT TCATGCAGCA AGGATGTGAC ATTTCTCCCA GGTCCCTGTC 
     5161 TGGTCGCATC GTTGGTGGCG TCTGGTGGTT CTTCACCTTA ATCATCATCT CCTCATATAC 
     5221 AGCCAATCTG GCCGCCTTCC TGACCGTGGA GAGGATGGTG TCTCCCATTG AGAGTGCAGA 
     5281 GGACCTAGCG AAGCAGACAG AAATTGCCTA CGGGACGCTG GAAGCAGGAT CTACTAAGGA 
     5341 GTTCTTCAGG AGGTCTAAAA TTGCTGTGTT TGAGAAGATG TGGACATACA TGAAGTCAGC 
     5401 AGAGCCATCA GTTTTTGTGC GGACCACAGA GGAGGGGATG ATTCGAGTGA GGAAATCCAA 
     5461 AGGCAAATAT GCCTACCTCC TGGAGTCCAC CATGAATGAG TACATTGAGC AGCGGAAACC 
     5521 CTGTGACACC ATGAAGGTGG GAGGTAACTT GGATTCCAAA GGCTATGGCA TTGCAACACC 
     5581 CAAGGGGTCT GCCCTGAGAG GTCCCGTAAA CCTAGCGGTT TTGAAACTCA GTGAGCAAGG 
     5641 CGTCTTAGAC AAGCTGAAAA GCAAATGGTG GTACGATAAA GGGGAATGTG GAAGCAAGGA 
     5701 CTCCGGAAGT AAGGACAAGA CAAGCGCTCT GAGCCTCAGC AATGTGGCAG GCGTGTTCTA 
     5761 CATCCTGATC GGAGGACTTG GACTAGCCAT GCTGGTTGCC TTAATCGAGT TCTGCTACAA 
     5821 ATCCCGTAGT GAATCCAAGC GGATGAAGGG TTTTTGTTTG ATCCCACAGC AATCCATCAA 
     5881 CGAAGCCATA CGGACATCGA CCCTCCCCCG CAACAGCGGG GCAGGAGCCA GCAGCGGCGG 
     5941 CAGTGGAGAG AATGGTCGGG TGGTCAGCCA TGACTTCCCC AAGTCCATGC AATCGATTCC 
     6001 TTGCATGAGC CACAGTTCAG GGATGCCCTT GGGAGCCACG GGATTGACGC GTACGCGGCC 
     6061 GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA 
     6121 GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG 
     6181 GGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG 
     6241 TTCCACTAGG TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 
     6301 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 
     6361 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 
     6421 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 
     6481 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 
     6541 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 
     6601 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 
     6661 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 
     6721 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 
     6781 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 
     6841 ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA 
     6901 ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA 
     6961 AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT 
     7021 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG 
     7081 GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG 
     7141 CTACTTCCCT GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT 
     7201 TGGATGGTGC TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG 
     7261 AGAGAACACC CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA 
     7321 AGTATTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA 
     7381 TCCGGACTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA 
     7441 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG 
     7501 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG 
     7561 GAAAATCTCT AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     7621 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     7681 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     7741 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     7801 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     7861 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     7921 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     7981 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     8041 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     8101 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     8161 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     8221 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     8281 TTAAGGGATT TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT 
     8341 TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC 
     8401 AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG 
     8461 AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG 
     8521 GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG 
     8581 TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA 
     8641 CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA 
     8701 CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC 
     8761 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG 
     8821 GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT 
     8881 GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT 
     8941 CCAGTGATTT TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     9001 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTC 
     9061 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAC
//