LOCUS RC226200 7377 bp DNA circular SYN DEFINITION VPS53 (Myc-DDK-tagged)-Human vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant 1 ACCESSION NM_001128159 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3534..3625 /label="Myc-DDK" misc_feature 3655..4240 /label="PolyA signal" misc_feature 3712..3731 /label="Restriction Enzyme" misc_feature 4516..5188 /label="ColE1" misc_feature 5618..6412 /label="Kan/Neo" misc_feature 6447..6749 /label="SV40 ori" misc_feature 6835..7312 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3516..3522 /label="Restriction Enzyme" misc_feature 1020..3516 /label="VPS53" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GATGGAGGAG GAGGAACTGG AGTTCGTGGA GGAGCTGGAA GCCGTGCTGC 1081 AGCTCACGCC CGAGGTGCAG CTGGCCATCG AGCAGGTGTT TCCAAGCCAG GACCCTCTAG 1141 ATCGAGCAGA TTTCAATGCT GTTGAGTATA TCAATACCCT GTTCCCAACC GAGCAATCTC 1201 TGGCGAACAT AGACGAAGTC GTGAACAAAA TTAGGCTGAA AATAAGGAGA CTGGATGACA 1261 ATATTCGAAC TGTTGTAAGA GGTCAGACGA ACGTGGGGCA GGATGGACGG CAAGCGCTTG 1321 AAGAGGCTCA GAAAGCTATC CAACAACTCT TTGGCAAAAT CAAAGATATC AAAGACAAAG 1381 CTGAAAAATC AGAGCAAATG GTGAAAGAAA TCACCCGTGA TATTAAGCAA TTAGATCACG 1441 CCAAACGCCA CCTGACCACC TCAATCACCA CACTGAACCA CCTGCACATG CTGGCAGGAG 1501 GTGTCGACTC CCTCGAAGCC ATGACCAGGC GAAGACAATA CGGAGAAGTT GCTAATCTCC 1561 TTCAGGGTGT GATGAATGTC CTGGAGCACT TCCACAAGTA TATGGGGATT CCGCAGATCC 1621 GGCAGCTTTC CGAAAGAGTG AAGGCTGCAC AGACTGAGTT AGGACAGCAA ATCCTGGCAG 1681 ATTTTGAAGA AGCGTTTCCT TCCCAGGGCA CCAAGAGACC AGGAGGACCC AGCAATGTTC 1741 TACGAGATGC ATGTCTGGTT GCTAATATTC TAGATCCCAG GATCAAACAG GAAATCATCA 1801 AAAAGTTTAT TAAACAGCAT CTGTCAGAGT ATCTGGTACT TTTTCAAGAA AACCAAGATG 1861 TTGCCTGGCT GGACAAAATC GACAGACGCT ATGCCTGGAT AAAACGCCAG CTTGTGGACT 1921 ATGAGGAGAA ATACGGCCGC ATGTTTCCAC GTGAGTGGTG CATGGCTGAG AGGATTGCGG 1981 TGGAATTTTG CCATGTGACA AGGGCAGAAC TTGCCAAGAT TATGCGTACC AGAGCGAAGG 2041 AAATTGAAGT GAAATTGCTT CTTTTTGCTA TTCAAAGAAC AACTAACTTT GAGGGGTTTC 2101 TTGCAAAACG CTTCTCCGGC TGCACCCTGA CCGATGGGAC CCTGAAAAAG CTTGAGTCTC 2161 CACCCCCATC TACCAATCCC TTCCTGGAAG ATGAGCCAAC ACCAGAGATG GAGGAACTGG 2221 CAACGGAGAA AGGAGATTTA GATCAACCAA AGAAGCCTAA AGCCCCAGAC AATCCATTTC 2281 ATGGCATTGT TTCCAAGTGT TTTGAGCCTC ATCTCTACGT GTATATCGAA TCCCAAGACA 2341 AGAACCTCGG AGAGCTGATA GATCGGTTTG TGGCTGATTT CAAAGCCCAG GGGCCACCTA 2401 AGCCCAACAC TGATGAAGGG GGTGCCGTGC TCCCCAGCTG CGCCGACCTC TTTGTCTACT 2461 ACAAGAAGTG CATGGTGCAA TGCTCTCAGC TCAGTACTGG GGAGCCCATG ATCGCCCTGA 2521 CCACCATTTT CCAGAAGTAC CTCCGAGAAT ACGCCTGGAA AATCCTCTCT GGCAACCTGC 2581 CCAAAACCAC AACCAGCAGT GGAGGACTGA CTATCAGCAG CCTCCTCAAG GAAAAGGAGG 2641 GCTCAGAAGT AGCCAAGTTC ACTCTGGAGG AGCTCTGCCT CATCTGTAAC ATCCTGAGCA 2701 CGGCAGAGTA CTGTCTGGCC ACCACCCAGC AGCTAGAAGA AAAACTCAAA GAAAAAGTGG 2761 ATGTAAGTCT GATTGAACGA ATCAATCTGA CTGGAGAGAT GGACACGTTC AGCACCGTCA 2821 TCTCCAGCAG TATTCAGCTG CTGGTTCAGG ATCTGGATGC TGCCTGTGAT CCTGCCCTGA 2881 CTGCCATGAG CAAGATGCAG TGGCAGAACG TGGAGCACGT TGGTGACCAG AGCCCCTACG 2941 TCACCTCTGT CATTCTGCAC ATCAAGCAGA ACGTCCCCAT CATCCGTGAC AACCTGGCTT 3001 CCACACGCAA GTACTTCACT CAGTTCTGCG TTAAATTTGC AAACTCCTTC ATTCCCAAAT 3061 TCATCACCCA CCTCTTCAAG TGCAAGCCAA TTAGCATGGT GGGAGCAGAA CAGCTGCTGC 3121 TGGACACCCA CTCGCTGAAG ATGGTCCTGC TCGATCTCCC CTCCATCAGC TCGCAGGTGG 3181 TGAGGAAGGC ACCCGCCAGC TACACCAAGA TCGTTGTCAA AGGCATGACC CGGGCTGAGA 3241 TGATCCTCAA GGTAGTGATG GCCCCTCATG AACCGTTGGT GGTGTTTGTT GACAACTACA 3301 TCAAACTTCT CACAGACTGC AACACAGAAA CCTTTCAGAA GATACTGGAC ATGAAGGGGC 3361 TGAAGAGGAG TGAGCAGAGC AGCATGCTGG AACTCCTGCG CCAGCGGCTC CCCGCACCGC 3421 CCTCGGGGGC AGAAAGCTCC GGCTCACTGT CCCTGACGGC GCCAACACCA GAGCAAGAGT 3481 CGTCACGCAT CCGCAAGCTC GAGAAACTCA TTAAAAAGAG ACTGACGCGT ACGCGGCCGC 3541 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 3601 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 3661 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 3721 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 3781 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 3841 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 3901 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 3961 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 4021 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 4081 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 4141 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 4201 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 4261 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 4321 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 4381 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 4441 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 4501 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 4561 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 4621 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 4681 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 4741 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 4801 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 4861 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 4921 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 4981 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 5041 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 5101 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 5161 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 5221 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 5281 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 5341 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 5401 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 5461 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 5521 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 5581 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 5641 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 5701 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 5761 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 5821 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 5881 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 5941 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 6001 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 6061 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 6121 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 6181 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 6241 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 6301 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 6361 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 6421 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 6481 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 6541 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 6601 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 6661 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 6721 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 6781 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 6841 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 6901 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 6961 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 7021 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 7081 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 7141 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 7201 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 7261 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 7321 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT //