LOCUS       RC226141L1                8640 bp    DNA     circular  SYN 
DEFINITION  PWWP2A (Myc-DDK-tagged)-Human PWWP domain containing 2A (PWWP2A), transcript variant 2
ACCESSION   NM_001130864
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6931..7111 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7839..8498 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7270..7838 
                     /label="ColE1"  
     misc_feature    5806..5826 
                     /label="LR50 primer"  
     misc_feature    5603..5674 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5815..6406 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5585..5591 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5585 
                     /label="PWWP2A"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGCCGTG GCTGCAGAGG CGGCAGCGAC 
     3361 TGCAGCGTCC CCCGGGGAGG GGGGCGCCGG CGAGGCCGAG CCGGAGATGG AGCCCATCCC 
     3421 CGGCAGTGAG GCCGGCACTG ACCCCCTCCC GGTCACGGCC ACTGAAGCGT CTGTGCCGGA 
     3481 TGGCGAGACT GACGGGCAGC AATCCGCTCC TCAGGCCGAC GAGCCGCCGC TCCCGCCGCC 
     3541 ACCGCCGCCG CCGGGGGAGC TCGCCCGCAG CCCAGAGGCG GTGGGGCCGG AGCTGGAGGC 
     3601 TGAGGAGAAA CTGTCCGTTC GGGTGGCGGA GTCGGCGGCA GCCGCGCCTC AGGGAGGGCC 
     3661 GGAACTTCCA CCTTCTCCTG CATCGCCGCC GGAGCAGCCC CCGGCTCCCG AGGAGCGCGA 
     3721 GGAGCCGCCG CTGCCTCAGC CCGTAGCCCC GGCGCTCGTG CCGCCGGCGG GCGGGGACTC 
     3781 CACGGTGTCG CAACTGATCC CGGGCTCGGA GGTGCGGGTC ACGCTGGACC ACATCATTGA 
     3841 GGACGCGCTT GTCGTGTCGT TCCGCTTCGG GGAGAAGCTC TTCTCCGGGG TCCTCATGGA 
     3901 TCTGTCCAAA AGGTTTGGGC CCCATGGTAT CCCTGTGACA GTATTTCCCA AAAGGGAATA 
     3961 TAAGGATAAA CCAGAAGCCA TGCCGCTCCA AAGTAATACA TTCCAAGAAG GGACAGAAGT 
     4021 CAAGTGTGAA GCAAATGGTG CTGTTCCCGA TGACCCTTCT CCTGTCCCGC ATCCCGAGCT 
     4081 GAGCTTGGCT GAAAGCCTGT GGACTTCCAA ACCACCACCT CTCTTCCATG AAGGAGCACC 
     4141 TTATCCTCCC CCTTTGTTTA TCAGGGACAC ATATAACCAA TCAATACCTC AGCCACCTCC 
     4201 TCGGAAAATT AAGCGACCCA AACGAAAAAT GTACAGGGAA GAACCCACTT CAATAATGAA 
     4261 TGCTATTAAA CTACGACCCA GGCAAGTTCT GTGTGATAAA TGTAAAAACA GTGTTGTTGC 
     4321 TGAAAAAAAG GAAATTAGAA AAGGTAGTAG TGCAACTGAC TCTTCTAAAT ATGAAGATAA 
     4381 AAAACGGAGA AATGAAAGTG TAACTACTGT GAACAAAAAA CTGAAAACTG ACCATAAAGT 
     4441 GGATGGGAAA AACCAAAATG AAAGCCAGAA AAGAAATGCT GTGGTTAAGG TTTCAAATAT 
     4501 TGCTCACAGC AGAGGCAGAG TAGTAAAAGT TTCTGCTCAG GCAAATACAT CAAAAGCTCA 
     4561 GTTAAGTACT AAAAAAGTTC TCCAGAGTAA GAACATGGAT CATGCGAAAG CTCGGGAAGT 
     4621 GTTAAAAATT GCCAAAGAAA AGGCACAAAA GAAGCAAAAT GAAACCTCTA CTTCCAAAAA 
     4681 TGCACATTCA AAAGTCCATT TCACACGTCG ATATCAGAAT CCTAGCTCAG GTTCCCTTCC 
     4741 ACCCCGGGTT CGTTTAAAAC CACAGAGGTA CAGGAATGAA GAAAATGACT CTTCTCTGAA 
     4801 GACAGGACTT GAGAAAATGC GGAGTGGCAA GATGGCACCC AAGCCCCAGT CTCGCTGCAC 
     4861 CTCTACCCGC TCAGCAGGTG AGGCCCCTTC AGAAAATCAG AGTCCCTCAA AAGGCCCTGA 
     4921 AGAGGCCAGC AGTGAGGTTC AGGACACAAA TGAAGTGCAT GTGCCTGGTG ATCAGGATGA 
     4981 ACCACAGACA TTGGGCAAAA AGGGCAGCAA AAACAATATC TCTGTTTATA TGACCCTAAA 
     5041 TCAAAAGAAA TCTGACTCTT CCAGTGCTTC AGTGTGTAGC ATTGATAGCA CAGATGATTT 
     5101 GAAATCTTCC AACTCTGAGT GTAGTTCTTC TGAAAGCTTT GATTTTCCTC CAGGCAGTAT 
     5161 GCATGCACCT TCCACCTCCT CCACTTCCTC CTCTTCAAAG GAAGAGAAAA AGCTCAGTAA 
     5221 TTCCTTGAAA ATGAAAGTCT TTTCCAAAAA CGTCTCTAAA TGCGTCACAC CAGATGGCAG 
     5281 GACCATATGT GTAGGGGACA TTGTTTGGGC CAAGATATAT GGCTTCCCTT GGTGGCCAGC 
     5341 CCGTATTCTT ACTATAACTG TGAGCCGGAA AGATAACGGC CTTTTAGTCC GACAGGAGGC 
     5401 CCGTATTTCA TGGTTTGGGT CTCCAACAAC ATCTTTCCTT GCTCTTTCAC AACTCTCCCC 
     5461 CTTTTTAGAA AACTTCCAGT CACGCTTTAA TAAGAAGAGA AAGGGCCTGT ATCGCAAGGC 
     5521 TATCACAGAG GCAGCTAAGG CTGCCAAGCA GCTGACCCCC GAAGTGCGGG CTTTGTTGAC 
     5581 ACAGTTTGAA ACGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT 
     5641 GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG 
     5701 CGGTCTGTAC AAGTAGGATT CGTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC 
     5761 GAAGTTATAC ATGTTTAAGG GTTCCGGTTC CACTAGGTAC AATTCGATAT CAAGCTTATC 
     5821 GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT 
     5881 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC 
     5941 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG 
     6001 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC 
     6061 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC 
     6121 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG 
     6181 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG 
     6241 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC 
     6301 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT 
     6361 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC 
     6421 CGTCGACCTC GATCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC 
     6481 TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT 
     6541 CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT 
     6601 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT 
     6661 TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG 
     6721 GCCAGGGATC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA 
     6781 AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT 
     6841 GCATGGGATG GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA GCCGCCTAGC 
     6901 ATTTCATCAC ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT GGTTAGACCA 
     6961 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG 
     7021 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG 
     7081 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGCATGTGAG CAAAAGGCCA 
     7141 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     7201 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     7261 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     7321 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     7381 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     7441 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     7501 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     7561 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     7621 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     7681 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     7741 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     7801 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGATTA CGCCCCGCCC 
     7861 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA 
     7921 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA 
     7981 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA 
     8041 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT 
     8101 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA 
     8161 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA 
     8221 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT 
     8281 GCCATACGGA ACTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA 
     8341 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 
     8401 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC 
     8461 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATACT CTTCCTTTTT 
     8521 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8581 ATTTAGAAAA ATAAACAAAT AGGGGTCCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//