LOCUS       RC226037                6750 bp    DNA     circular  SYN 
DEFINITION  TKT (Myc-DDK-tagged)-Human transketolase (TKT), transcript variant 2
ACCESSION   NM_001135055
VERSION     NM_001135055.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2907..2998 
                     /label="Myc-DDK"  
     misc_feature    3028..3613 
                     /label="PolyA signal"  
     misc_feature    3085..3104 
                     /label="Restriction Enzyme"  
     misc_feature    3889..4561 
                     /label="ColE1"  
     misc_feature    4991..5785 
                     /label="Kan/Neo"  
     misc_feature    5820..6122 
                     /label="SV40 ori"  
     misc_feature    6208..6685 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2889..2895 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2889 
                     /label="TKT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGCTAC CACAAGCCTG ACCAGCAGAA GCTGCAGGCC TTGAAGGACA 
     1081 CGGCCAACCG CCTACGTATC AGCTCCATCC AGGCCACCAC TGCGGCGGGC TCTGGCCACC 
     1141 CCACGTCATG CTGCAGCGCC GCAGAGATCA TGGCTGTCCT CTTTTTCCAC ACCATGCGCT 
     1201 ACAAGTCCCA GGACCCCCGG AATCCGCACA ATGACCGCTT TGTGCTCTCC AAGGGCCATG 
     1261 CAGCTCCCAT CCTCTACGCG GTCTGGGCTG AAGCTGGTTT CCTGGCCGAG GCGGAGCTGC 
     1321 TGAACCTGAG GAAGATCAGC TCCGACTTGG ACGGGCACCC GGTCCCGAAA CAAGCTTTCA 
     1381 CCGACGTGGC CACTGGCTCC CTGGGCCAGG GCCTCGGGGC CGCTTGTGGG ATGGCCTACA 
     1441 CCGGCAAATA CTTCGACAAG GCCAGCTACC GAGTCTATTG CTTGCTGGGA GATGGGGAGC 
     1501 TGTCAGAGGG CTCTGTATGG GAGGCCATGG CCTTCGCCAG CATCTATAAG CTGGACAACC 
     1561 TTGTGGCCAT TCTAGACATC AATCGCCTGG GCCAGAGTGA CCCGGCCCCG CTGCAGCACC 
     1621 AGATGGACAT CTACCAGAAG CGGTGCGAGG CCTTCGGTTG GCATGCCATC ATCGTGGATG 
     1681 GACACAGCGT GGAGGAGCTG TGCAAGGCCT TTGGCCAGGC CAAGCACCAG CCAACAGCCA 
     1741 TCATTGCCAA GACCTTCAAG GGCCGAGGGA TCACGGGGGT AGAAGATAAG GAGTCTTGGC 
     1801 ATGGGAAGCC CCTCCCCAAA AACATGGCTG AGCAGATCAT CCAGGAGATC TACAGCCAGA 
     1861 TCCAGAGCAA AAAGAAGATC CTGGCAACCC CTCCACAGGA GGACGCACCC TCAGTGGACA 
     1921 TTGCCAACAT CCGCATGCCC AGCCTGCCCA GCTACAAAGT TGGGGACAAG ATAGCCACCC 
     1981 GCAAGGCCTA CGGGCAGGCA CTGGCCAAGC TGGGCCATGC CAGTGACCGC ATCATCGCCC 
     2041 TGGATGGGGA CACCAAAAAT TCCACCTTCT CGGAGATCTT CAAAAAGGAG CACCCGGACC 
     2101 GCTTCATCGA GTGCTACATT GCCGAGCAGA ACATGGTGAG CATCGCGGTG GGCTGTGCCA 
     2161 CCCGCAACAG GACGGTGCCC TTCTGCAGCA CTTTTGCAGC CTTCTTCACG CGGGCCTTTG 
     2221 ACCAGATTCG CATGGCCGCC ATCTCCGAGA GCAACATCAA CCTCTGCGGC TCCCACTGCG 
     2281 GCGTTTCCAT CGGGGAAGAC GGGCCCTCCC AGATGGCCCT AGAAGATCTG GCTATGTTTC 
     2341 GGTCAGTCCC CACATCAACT GTCTTTTACC CAAGTGATGG CGTTGCTACA GAGAAGGCAG 
     2401 TGGAACTAGC CGCCAATACA AAGGGTATCT GCTTCATCCG GACCAGCCGC CCAGAAAATG 
     2461 CCATCATCTA TAACAACAAT GAGGACTTCC AGGTCGGACA AGCCAAGGTG GTCCTGAAGA 
     2521 GCAAGGATGA CCAGGTGACC GTTATCGGGG CTGGGGTGAC CCTGCACGAG GCCTTGGCCG 
     2581 CTGCCGAACT GCTGAAGAAA GAAAAGATCA ACATCCGCGT GCTGGACCCC TTCACCATCA 
     2641 AGCCCCTGGA CAGAAAACTC ATTCTCGACA GCGCTCGTGC CACCAAGGGC AGGATCCTCA 
     2701 CCGTGGAGGA CCATTATTAT GAAGGTGGCA TTGGTGAGGC TGTGTCCAGT GCAGTAGTGG 
     2761 GCGAGCCTGG CATCACTGTC ACCCACCTGG CAGTTAACCG GGTACCAAGA AGTGGGAAGC 
     2821 CAGCTGAGCT GCTGAAGATG TTTGGTATCG ACAGGGATGC CATTGCACAA GCTGTGAGGG 
     2881 GCCTCATCAC CAAGGCCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     2941 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3001 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3061 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3121 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3181 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3241 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3301 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3361 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3421 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3481 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     3541 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     3601 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     3661 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     3721 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     3781 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     3841 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     3901 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     3961 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4021 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4081 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4141 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4201 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4261 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4321 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4381 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4441 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4501 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     4561 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     4621 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     4681 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     4741 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     4801 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     4861 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     4921 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     4981 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5041 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5101 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5161 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5221 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5281 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5341 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5401 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5461 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     5521 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     5581 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     5641 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     5701 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     5761 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     5821 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     5881 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     5941 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6001 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6061 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6121 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6181 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6241 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6301 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6361 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6421 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6481 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6541 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     6601 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     6661 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     6721 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//