LOCUS       RC225897                6453 bp    DNA     circular  SYN 
DEFINITION  CYP4F11 (Myc-DDK-tagged)-Human cytochrome P450, family 4, subfamily F, polypeptide 11 (CYP4F11), transcript variant 2
ACCESSION   NM_001128932
VERSION     NM_001128932.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2610..2701 
                     /label="Myc-DDK"  
     misc_feature    2731..3316 
                     /label="PolyA signal"  
     misc_feature    2788..2807 
                     /label="Restriction Enzyme"  
     misc_feature    3592..4264 
                     /label="ColE1"  
     misc_feature    4694..5488 
                     /label="Kan/Neo"  
     misc_feature    5523..5825 
                     /label="SV40 ori"  
     misc_feature    5911..6388 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2592..2598 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2592 
                     /label="CYP4F11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGCAGCTG AGCCTGTCCT GGCTGGGCCT CGGGCCCGTG GCAGCATCCC 
     1081 CGTGGCTGCT TCTGCTGCTG GTTGGGGGCT CCTGGCTCCT GGCCCGCGTC CTGGCCTGGA 
     1141 CCTACACCTT CTATGACAAC TGCCGCCGCC TCCAGTGTTT TCCTCAACCC CCGAAACAGA 
     1201 ACTGGTTTTG GGGACACCAG GGCCTGGTCA CTCCCACGGA AGAGGGCATG AAGACATTGA 
     1261 CCCAGCTGGT GACCACATAT CCCCAGGGCT TTAAGTTGTG GCTGGGTCCT ACCTTCCCCC 
     1321 TCCTCATTTT ATGCCACCCT GACATCATCC GGCCTATCAC CAGTGCCTCA GCTGCTGTCG 
     1381 CACCCAAGGA TATGATTTTC TATGGCTTCC TGAAGCCCTG GCTGGGGGAT GGGCTCCTGC 
     1441 TGAGTGGTGG TGACAAGTGG AGCCGCCACC GTCGGATGTT GACGCCTGCC TTCCATTTCA 
     1501 ACATCTTGAA GCCTTATATG AAGATTTTCA ACAAGAGTGT GAACATCATG CACGACAAGT 
     1561 GGCAGCGCCT GGCCTCAGAG GGCAGCGCCA GACTGGACAT GTTTGAACAC ATCAGCCTCA 
     1621 TGACCTTGGA CAGTCTGCAG AAATGTGTCT TCAGCTTTGA AAGCAATTGT CAGGAGAAGC 
     1681 CCAGTGAATA TATTGCCGCC ATCTTGGAGC TCAGTGCCTT TGTAGAAAAG AGAAACCAGC 
     1741 AGATTCTCTT GCACACGGAC TTCCTGTATT ATCTCACTCC TGATGGGCAG CGCTTCCGCA 
     1801 GGGCCTGCCA CCTGGTGCAC GACTTCACAG ATGCCGTCAT CCAGGAGCGG CGCCGCACCC 
     1861 TCCCCACTCA GGGTATTGAT GATTTCCTCA AGAACAAGGC AAAGTCCAAG ACTTTAGACT 
     1921 TCATTGATGT GCTTCTGCTG AGCAAGGATG AAGATGGGAA GGAATTGTCT GATGAGGACA 
     1981 TAAGAGCAGA AGCTGACACC TTCATGTTTG AGGGCCATGA CACTACAGCC AGTGGTCTCT 
     2041 CCTGGGTCCT ATACCACCTT GCAAAGCACC CAGAATACCA GGAACAGTGC CGGCAAGAAG 
     2101 TGCAAGAGCT TCTGAAGGAC CGTGAACCTA TAGAGATTGA ATGGGACGAC CTGGCCCAGC 
     2161 TGCCCTTCCT GACCATGTGC ATTAAGGAGA GCCTGCGGTT GCATCCCCCA GTCCCGGTCA 
     2221 TCTCCCGATG TTGCACGCAG GACTTTGTGC TCCCAGACGG CCGCGTCATC CCCAAAGGCA 
     2281 TTGTCTGCCT CATCAATATT ATCGGGATCC ATTACAACCC AACTGTGTGG CCAGACCCTG 
     2341 AGGTCTACGA CCCCTTCCGT TTCAACCAAG AGAACATCAA GGAGAGGTCA CCTCTGGCTT 
     2401 TTATTCCCTT CTCGGCAGGG CCCAGAAACT GCATCGGGCA GGCGTTCGCC ATGGCTGAGA 
     2461 TGAAGGTGGT CCTGGCGCTC ACGCTGCTGC ACTTCCGCAT CCTGCCGACC CACACTGAAC 
     2521 CCCGCAGGAA ACCCGAGCTG ATATTGCGCG CAGAGGGTGG ACTTTGGCTG CGGGTGGAGC 
     2581 CCCTGGGTGC GAACTCACAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     2641 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     2701 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     2761 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     2821 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     2881 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     2941 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3001 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3061 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3121 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3181 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3241 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3301 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3361 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3421 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     3481 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     3541 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     3601 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     3661 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     3721 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     3781 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     3841 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     3901 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     3961 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4021 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4081 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4141 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4201 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4261 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4321 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4381 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4441 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     4501 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     4561 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     4621 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     4681 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     4741 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     4801 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     4861 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     4921 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     4981 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5041 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5101 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5161 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5221 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5281 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5341 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5401 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     5461 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     5521 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     5581 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     5641 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     5701 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     5761 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     5821 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     5881 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     5941 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6001 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6061 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6121 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6181 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6241 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6301 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6361 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6421 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
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