LOCUS       RC224956                8772 bp    DNA     circular  SYN 
DEFINITION  INSRR (Myc-DDK-tagged)-Human insulin receptor-related receptor (INSRR)
ACCESSION   NM_014215
VERSION     NM_014215.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4929..5020 
                     /label="Myc-DDK"  
     misc_feature    5050..5635 
                     /label="PolyA signal"  
     misc_feature    5107..5126 
                     /label="Restriction Enzyme"  
     misc_feature    5911..6583 
                     /label="ColE1"  
     misc_feature    7013..7807 
                     /label="Kan/Neo"  
     misc_feature    7842..8144 
                     /label="SV40 ori"  
     misc_feature    8230..8707 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4911..4917 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4911 
                     /label="INSRR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGTGCCT AGTCTGTGGC CCTGGGGAGC ATGCCTGCCT GTGATCTTCC 
     1081 TCTCCTTGGG ATTTGGCCTG GATACAGTAG AGGTGTGCCC CAGCCTGGAT ATTCGCTCAG 
     1141 AGGTGGCAGA GCTTCGTCAG CTGGAGAACT GCAGCGTGGT GGAGGGCCAC CTGCAGATCC 
     1201 TGCTCATGTT CACAGCCACC GGGGAGGACT TCCGCGGCCT CAGCTTCCCT CGCCTCACCC 
     1261 AGGTCACCGA CTACCTGCTG CTCTTCCGTG TCTACGGACT GGAGAGCCTG CGCGACCTCT 
     1321 TCCCCAACCT AGCAGTCATC CGCGGGACGC GCCTCTTCCT GGGCTATGCA CTGGTCATCT 
     1381 TTGAGATGCC ACATCTGCGT GACGTGGCAC TGCCTGCACT TGGGGCCGTG CTGCGTGGGG 
     1441 CTGTGCGTGT GGAGAAGAAC CAGGAGCTCT GCCACCTCTC CACCATTGAC TGGGGACTGC 
     1501 TGCAGCCAGC ACCTGGCGCC AACCACATCG TGGGCAACAA GCTGGGCGAG GAGTGTGCTG 
     1561 ACGTGTGCCC TGGTGTGCTG GGTGCTGCTG GTGAGCCCTG TGCCAAGACC ACCTTCAGCG 
     1621 GGCACACTGA CTACAGATGC TGGACCTCCA GCCACTGCCA GAGAGTGTGC CCCTGCCCCC 
     1681 ATGGGATGGC TTGCACAGCG AGGGGCGAGT GCTGCCACAC CGAATGCCTG GGGGGCTGCA 
     1741 GCCAGCCAGA AGACCCTCGT GCCTGTGTAG CTTGCCGCCA CCTCTACTTC CAGGGTGCCT 
     1801 GCCTGTGGGC CTGCCCGCCA GGCACCTACC AGTATGAGTC CTGGCGCTGT GTCACAGCTG 
     1861 AGCGCTGTGC CAGCCTGCAC TCTGTGCCCG GCCGTGCCTC CACCTTCGGC ATACACCAGG 
     1921 GCAGTTGCCT GGCCCAGTGC CCTTCTGGCT TCACCCGTAA TAGCAGCAGC ATATTCTGCC 
     1981 ACAAGTGCGA GGGGCTGTGC CCTAAAGAGT GCAAGGTAGG CACCAAGACC ATCGACTCCA 
     2041 TCCAGGCGGC ACAGGATCTT GTGGGCTGCA CGCATGTGGA GGGAAGCCTC ATCCTCAACC 
     2101 TTCGCCAGGG CTACAACCTG GAGCCACAGC TGCAGCACAG CCTGGGGCTG GTAGAAACCA 
     2161 TTACTGGCTT CCTCAAAATC AAGCACTCCT TTGCCCTCGT GTCCCTGGGC TTTTTCAAGA 
     2221 ACCTCAAACT AATCCGGGGA GACGCCATGG TGGATGGGAA CTACACTCTC TACGTGCTGG 
     2281 ACAACCAGAA CCTACAACAG CTAGGGTCCT GGGTGGCCGC GGGGCTCACC ATTCCCGTGG 
     2341 GCAAGATCTA CTTCGCCTTC AACCCGCGCC TCTGCTTGGA ACACATCTAC CGACTGGAGG 
     2401 AGGTGACAGG CACGCGAGGT CGGCAGAACA AGGCTGAGAT CAACCCCCGC ACCAACGGAG 
     2461 ACCGCGCCGC CTGCCAGACT CGCACCCTGC GCTTCGTGTC CAACGTGACG GAGGCAGACC 
     2521 GCATCCTGCT ACGCTGGGAG CGCTATGAGC CACTGGAGGC CCGCGACCTG CTCAGCTTCA 
     2581 TCGTGTACTA CAAGGAGTCC CCATTCCAGA ACGCCACAGA GCACGTGGGT CCAGATGCTT 
     2641 GTGGAACCCA GAGCTGGAAC CTGCTGGATG TGGAGCTGCC CCTAAGCCGC ACCCAGGAGC 
     2701 CAGGGGTGAC CCTAGCCTCC CTCAAGCCTT GGACACAGTA CGCAGTGTTT GTGCGGGCCA 
     2761 TCACGCTAAC CACTGAGGAG GACAGCCCTC ATCAAGGAGC CCAGAGTCCC ATCGTCTACC 
     2821 TCCGAACGCT GCCTGCAGCT CCCACGGTGC CCCAAGACGT CATCTCCACG TCCAACTCCT 
     2881 CCTCCCACCT CCTGGTGCGC TGGAAGCCAC CGACCCAGCG CAATGGGAAC CTCACCTACT 
     2941 ACCTGGTGCT GTGGCAGCGG CTGGCAGAGG ACGGCGACCT CTACCTCAAT GACTACTGCC 
     3001 ACCGCGGCTT GCGGCTGCCC ACCAGCAACA ACGATCCGCG CTTCGACGGC GAAGACGGGG 
     3061 ATCCTGAGGC CGAGATGGAG TCCGACTGCT GCCCTTGCCA GCACCCACCT CCTGGTCAGG 
     3121 TTCTGCCCCC GCTGGAGGCG CAAGAGGCCT CGTTCCAGAA GAAGTTTGAA AACTTTCTAC 
     3181 ACAACGCGAT CACCATCCCC ATATCCCCTT GGAAGGTGAC GTCCATCAAC AAGAGCCCCC 
     3241 AAAGGGACTC AGGGCGGCAC CGCCGGGCAG CTGGGCCCCT CCGGCTGGGG GGCAACAGCT 
     3301 CGGATTTCGA GATCCAGGAG GACAAGGTGC CCCGTGAGCG AGCGGTGCTG AGCGGCCTGC 
     3361 GCCACTTCAC GGAATACCGG ATCGACATCC ATGCCTGCAA CCACGCGGCG CACACCGTGG 
     3421 GCTGCAGCGC CGCCACCTTC GTCTTTGCGC GCACCATGCC CCACAGAGAG GCTGATGGTA 
     3481 TTCCAGGAAA GGTGGCCTGG GAGGCCTCCA GCAAGAACAG TGTCCTTCTG CGCTGGCTCG 
     3541 AGCCACCAGA CCCCAACGGA CTCATCCTCA AGTACGAAAT CAAGTACCGC CGCTTGGGAG 
     3601 AGGAGGCCAC AGTGCTGTGT GTGTCCCGTC TTCGATATGC GAAGTTTGGG GGAGTCCACC 
     3661 TGGCCCTGCT GCCCCCTGGA AACTACTCTG CCAGGGTTAG GGCAACCTCA CTGGCTGGCA 
     3721 ATGGCTCTTG GACAGACAGT GTTGCCTTCT ACATCCTTGG CCCAGAGGAG GAGGATGCTG 
     3781 GGGGGCTGCA TGTCCTCCTC ACTGCCACCC CTGTGGGGCT CACGCTGCTC ATCGTTCTTG 
     3841 CTGCCCTTGG TTTCTTCTAC GGCAAGAAGA GAAACAGAAC CCTGTATGCT TCTGTGAATC 
     3901 CAGAGTACTT CAGCGCCTCT GATATGTATG TCCCTGATGA ATGGGAGGTG CCTCGGGAGC 
     3961 AGATCTCGAT AATCCGGGAA CTGGGCCAGG GCTCTTTTGG GATGGTATAT GAGGGGCTGG 
     4021 CACGAGGACT TGAGGCTGGA GAGGAGTCCA CACCCGTGGC CCTGAAGACG GTGAATGAGC 
     4081 TGGCCAGCCC ACGGGAATGC ATTGAGTTCC TCAAGGAAGC TTCTGTCATG AAAGCCTTCA 
     4141 AGTGTCACCA TGTGGTGCGT CTCCTGGGTG TGGTATCTCA GGGCCAGCCA ACTCTGGTCA 
     4201 TCATGGAGTT AATGACCCGT GGGGACCTCA AGAGCCATCT TCGATCTTTG CGGCCTGAGG 
     4261 CAGAGAACAA CCCTGGGCTC CCACAGCCAG CATTGGGGGA AATGATCCAA ATGGCTGGTG 
     4321 AGATTGCAGA CGGCATGGCC TACCTTGCTG CCAACAAGTT TGTGCACCGA GATCTAGCAG 
     4381 CCCGCAACTG CATGGTGTCC CAGGACTTCA CCGTCAAGAT CGGGGACTTC GGGATGACTC 
     4441 GGGACGTGTA TGAGACAGAC TATTACCGCA AGGGTGGGAA GGGGCTGCTG CCCGTGCGCT 
     4501 GGATGGCCCC CGAGTCCCTC AAAGATGGGA TCTTCACCAC CCACTCGGAT GTCTGGTCCT 
     4561 TTGGCGTGGT ACTCTGGGAG ATTGTGACCC TGGCAGAACA ACCCTACCAG GGCCTGTCCA 
     4621 ATGAGCAGGT GCTGAAGTTC GTCATGGATG GCGGGGTCCT GGAGGAGCTG GAGGGCTGTC 
     4681 CCCTTCAGCT GCAGGAGCTG ATGAGCCGCT GCTGGCAGCC GAACCCACGC CTGCGCCCAT 
     4741 CTTTCACACA CATTCTGGAC AGCATACAGG AGGAGCTGCG GCCCTCCTTC CGCCTCCTCT 
     4801 CCTTCTACTA CAGCCCGGAA TGCCGGGGGG CCCGGGGCTC CCTGCCTACC ACCGATGCAG 
     4861 AGCCTGACTC CTCACCCACT CCAAGAGACT GCAGCCCTCA AAATGGGGGT CCAGGGCACA 
     4921 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4981 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5041 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5101 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5161 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5221 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5281 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5341 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5401 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5461 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5521 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5581 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5641 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5701 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5761 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5821 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5881 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5941 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6001 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6061 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6121 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6181 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6241 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6301 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6361 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6421 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6481 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6541 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6601 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6661 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6721 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6781 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6841 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6901 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6961 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7021 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7081 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7141 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7201 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7261 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7321 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7381 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7441 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7501 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7561 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7621 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7681 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7741 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7801 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7861 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7921 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7981 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8041 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8101 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8161 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8221 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8281 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8341 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8401 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8461 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8521 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8581 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8641 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8701 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8761 AACGCGAATT TT
//