LOCUS       RC224941                8373 bp    DNA     circular  SYN 
DEFINITION  ATP8A1 (Myc-DDK-tagged)-Human ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 (ATP8A1), transcript variant 1
ACCESSION   NM_006095
VERSION     NM_006095.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4530..4621 
                     /label="Myc-DDK"  
     misc_feature    4651..5236 
                     /label="PolyA signal"  
     misc_feature    4708..4727 
                     /label="Restriction Enzyme"  
     misc_feature    5512..6184 
                     /label="ColE1"  
     misc_feature    6614..7408 
                     /label="Kan/Neo"  
     misc_feature    7443..7745 
                     /label="SV40 ori"  
     misc_feature    7831..8308 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4512..4518 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4512 
                     /label="ATP8A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCACCATG CGGAGGACCG TGTCGGAGAT CCGCTCGCGC GCCGAAGGTT 
     1081 ATGAGAAGAC AGATGATGTT TCAGAGAAGA CCTCACTGGC TGACCAGGAG GAAGTAAGGA 
     1141 CTATTTTCAT CAACCAGCCC CAGCTGACAA AATTCTGCAA TAACCATGTC AGCACTGCAA 
     1201 AATACAACAT AATCACATTC CTTCCAAGAT TTCTCTACTC TCAGTTCAGA AGAGCTGCTA 
     1261 ATTCATTTTT TCTCTTTATT GCACTGCTGC AGCAAATACC TGATGTGTCA CCAACAGGTC 
     1321 GTTATACAAC ACTGGTTCCT CTCTTATTTA TTTTAGCTGT GGCAGCTATC AAAGAGATAA 
     1381 TAGAAGATAT TAAACGACAT AAAGCTGATA ATGCAGTGAA CAAGAAACAA ACGCAAGTTT 
     1441 TGAGAAATGG TGCTTGGGAA ATTGTCCACT GGGAAAAGGT GGCAGTAGGG GAGATAGTGA 
     1501 AAGTGACCAA TGGGGAACAT CTCCCAGCAG ATCTCATCAG TCTGTCCTCA AGTGAGCCCC 
     1561 AAGCCATGTG CTACATTGAA ACATCCAACT TAGATGGTGA AACAAACTTG AAAATTAGAC 
     1621 AGGGCTTACC AGCAACATCA GATATCAAAG ACGTTGACAG TTTGATGAGG ATTTCTGGCA 
     1681 GAATTGAGTG TGAAAGTCCA AACAGACATC TCTACGATTT TGTTGGAAAC ATAAGGCTTG 
     1741 ATGGACATGG CACCGTTCCA CTGGGAGCAG ATCAGATTCT TCTTCGAGGA GCTCAGTTGA 
     1801 GAAATACACA GTGGGTTCAT GGAATAGTTG TCTACACTGG ACATGACACC AAGCTGATGC 
     1861 AGAATTCAAC AAGTCCACCA CTTAAGCTCT CAAATGTGGA ACGGATTACA AATGTACAAA 
     1921 TTTTGATTTT ATTTTGTATC TTAATTGCCA TGTCTCTTGT CTGTTCTGTG GGCTCAGCCA 
     1981 TTTGGAATCG AAGGCATTCT GGAAAAGACT GGTATCTCAA TCTAAACTAT GGTGGCGCTA 
     2041 GTAATTTTGG ACTGAATTTC TTGACCTTCA TCATCCTTTT CAACAATCTC ATTCCTATCA 
     2101 GCTTATTGGT TACATTAGAA GTTGTGAAAT TTACCCAGGC ATACTTCATA AATTGGGATC 
     2161 TTGACATGCA CTATGAACCC ACAGACACTG CTGCTATGGC TCGAACATCT AATCTGAATG 
     2221 AGGAACTTGG CCAGGTTAAA TACATATTTT CTGACAAAAC TGGTACTCTG ACATGCAATG 
     2281 TAATGCAGTT TAAGAAGTGC ACCATAGCGG GAGTTGCTTA TGGCCATGTC CCTGAACCTG 
     2341 AGGATTATGG CTGCTCTCCT GATGAATGGC AGAACTCACA GTTTGGAGAT GAAAAAACAT 
     2401 TTAGTGATTC ATCATTGCTG GAAAATCTCC AAAATAATCA TCCAACTGCA CCTATAATAT 
     2461 GTGAATTTCT TACAATGATG GCAGTCTGTC ACACAGCAGT GCCAGAGCGA GAAGGTGACA 
     2521 AGATTATTTA TCAAGCAGCA TCTCCAGATG AGGGAGCATT GGTCAGAGCA GCCAAGCAAT 
     2581 TGAATTTTGT TTTCACTGGA AGAACACCCG ACTCGGTGAT TATAGATTCA CTGGGGCAGG 
     2641 AAGAAAGATA TGAATTGCTC AATGTCTTGG AGTTTACCAG TGCTAGGAAA AGAATGTCAG 
     2701 TGATTGTTCG CACTCCATCT GGAAAGTTAC GACTCTACTG CAAAGGAGCT GACACTGTAA 
     2761 TTTATGATCG ACTGGCAGAG ACGTCAAAAT ACAAAGAAAT TACCCTAAAA CATTTAGAGC 
     2821 AGTTTGCTAC AGAAGGGTTA AGAACTTTAT GTTTTGCTGT GGCTGAGATT TCAGAGAGCG 
     2881 ACTTTCAGGA GTGGCGAGCA GTCTATCAGC GAGCATCTAC ATCTGTGCAG AACAGGCTAC 
     2941 TCAAACTCGA AGAGAGTTAT GAGTTGATTG AAAAGAATCT TCAGCTACTT GGAGCAACAG 
     3001 CCATTGAGGA TAAATTACAA GATCAAGTGC CTGAAACCAT AGAAACGCTA ATGAAAGCAG 
     3061 ACATCAAAAT CTGGATCCTT ACAGGGGACA AGCAAGAAAC TGCCATTAAC ATCGGACACT 
     3121 CCTGCAAACT GTTGAAGAAG AACATGGGAA TGATTGTTAT AAATGAAGGC TCTCTTGATG 
     3181 GAACAAGGGA AACTCTCAGT CGTCACTGTA CTACCCTTGG TGATGCTCTC CGGAAAGAGA 
     3241 ATGATTTTGC TCTTATAATT GATGGGAAAA CCCTCAAATA TGCCTTAACC TTTGGAGTAC 
     3301 GACAGTATTT CCTGGACTTA GCTTTGTCAT GCAAAGCTGT CATTTGCTGT CGGGTTTCTC 
     3361 CTCTTCAAAA ATCTGAAGTT GTTGAGATGG TTAAGAAACA AGTCAAAGTC GTAACGCTTG 
     3421 CAATCGGTGA TGGAGCAAAT GATGTCAGCA TGATACAGAC AGCGCACGTT GGTGTTGGTA 
     3481 TCAGTGGCAA TGAAGGCCTG CAGGCAGCTA ATTCCTCTGA CTACTCCATA GCTCAGTTCA 
     3541 AATATTTGAA GAATTTACTG ATGATTCATG GTGCCTGGAA CTATAACAGA GTCTCCAAGT 
     3601 GCATCTTATA CTGCTTCTAC AAGAATATAG TGCTCTATAT TATCGAGATC TGGTTTGCCT 
     3661 TTGTTAATGG CTTTTCTGGA CAGATCCTCT TTGAAAGATG GTGTATAGGT CTCTATAACG 
     3721 TGATGTTTAC AGCAATGCCT CCTTTAACTC TTGGAATATT TGAGAGATCA TGCAGAAAAG 
     3781 AGAACATGTT GAAGTACCCT GAATTATACA AAACATCTCA GAATGCCCTG GACTTCAACA 
     3841 CCAAGGTTTT CTGGGTTCAT TGTTTAAATG GCCTCTTCCA CTCAGTTATT CTGTTTTGGT 
     3901 TTCCACTAAA AGCCCTTCAG TATGGTACTG CATTTGGAAA TGGGAAAACC TCGGATTATC 
     3961 TGCTACTGGG AAACTTTGTG TACACTTTTG TGGTGATAAC TGTGTGTTTG AAAGCTGGAT 
     4021 TGGAGACATC ATATTGGACA TGGTTCAGCC ACATAGCGAT ATGGGGGAGC ATCGCACTCT 
     4081 GGGTGGTGTT TTTTGGAATC TACTCATCTC TGTGGCCTGC CATTCCGATG GCCCCTGATA 
     4141 TGTCAGGAGA GGCAGCCATG TTGTTCAGTT CTGGAGTCTT TTGGATGGGC TTGTTATTCA 
     4201 TCCCTGTGGC ATCTCTGCTC CTTGATGTGG TGTACAAGGT TATCAAGAGG ACTGCTTTTA 
     4261 AAACATTGGT CGATGAAGTT CAGGAGCTGG AGGCAAAATC TCAAGACCCA GGAGCAGTTG 
     4321 TACTTGGAAA AAGCCTGACC GAGAGGGCGC AACTGCTCAA GAACGTCTTT AAGAAGAACC 
     4381 ACGTGAACTT GTACCGCTCT GAATCCTTGC AACAAAATCT GCTCCATGGG TATGCGTTCT 
     4441 CTCAAGATGA AAATGGAATC GTTTCACAGT CTGAAGTGAT AAGAGCATAT GATACCACGA 
     4501 AACAGAGGCC CGACGAATGG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4561 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4621 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4681 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4741 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4801 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4861 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4921 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4981 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5041 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5101 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5161 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5221 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5281 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5341 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5401 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5461 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5521 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5581 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5641 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5701 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5761 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5821 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5881 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5941 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6001 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6061 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6121 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6181 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6241 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6301 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6361 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6421 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6481 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6541 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6601 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6661 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6721 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6781 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6841 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6901 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6961 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7021 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7081 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7141 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7201 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7261 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7321 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7381 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7441 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7501 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7561 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7621 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7681 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7801 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7861 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7921 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7981 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8041 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8101 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8161 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8221 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8281 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8341 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//