LOCUS       RC224901                6786 bp    DNA     circular  SYN 
DEFINITION  MPL (Myc-DDK-tagged)-Human myeloproliferative leukemia virus oncogene (MPL)
ACCESSION   NM_005373
VERSION     NM_005373.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2943..3034 
                     /label="Myc-DDK"  
     misc_feature    3064..3649 
                     /label="PolyA signal"  
     misc_feature    3121..3140 
                     /label="Restriction Enzyme"  
     misc_feature    3925..4597 
                     /label="ColE1"  
     misc_feature    5027..5821 
                     /label="Kan/Neo"  
     misc_feature    5856..6158 
                     /label="SV40 ori"  
     misc_feature    6244..6721 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2925..2931 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2925 
                     /label="MPL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCTCCTGG GCCCTCTTCA TGGTCACCTC CTGCCTCCTC CTGGCCCCTC 
     1081 AAAACCTGGC CCAAGTCAGC AGCCAAGATG TCTCCTTGCT GGCATCAGAC TCAGAGCCCC 
     1141 TGAAGTGTTT CTCCCGAACA TTTGAGGACC TCACTTGCTT CTGGGATGAG GAAGAGGCAG 
     1201 CGCCCAGTGG GACATACCAG CTGCTGTATG CCTACCCGCG GGAGAAGCCC CGTGCTTGCC 
     1261 CCCTGAGTTC CCAGAGCATG CCCCACTTTG GAACCCGATA CGTGTGCCAG TTTCCAGACC 
     1321 AGGAGGAAGT GCGTCTCTTC TTTCCGCTGC ACCTCTGGGT GAAGAATGTG TTCCTAAACC 
     1381 AGACTCGGAC TCAGCGAGTC CTCTTTGTGG ACAGTGTAGG CCTGCCGGCT CCCCCCAGTA 
     1441 TCATCAAGGC CATGGGTGGG AGCCAGCCAG GGGAACTTCA GATCAGCTGG GAGGAGCCAG 
     1501 CTCCAGAAAT CAGTGATTTC CTGAGGTACG AACTCCGCTA TGGCCCCAGA GATCCCAAGA 
     1561 ACTCCACTGG TCCCACGGTC ATACAGCTGA TTGCCACAGA AACCTGCTGC CCTGCTCTGC 
     1621 AGAGGCCTCA CTCAGCCTCT GCTCTGGACC AGTCTCCATG TGCTCAGCCC ACAATGCCCT 
     1681 GGCAAGATGG ACCAAAGCAG ACCTCCCCAA GTAGAGAAGC TTCAGCTCTG ACAGCAGAGG 
     1741 GTGGAAGCTG CCTCATCTCA GGACTCCAGC CTGGCAACTC CTACTGGCTG CAGCTGCGCA 
     1801 GCGAACCTGA TGGGATCTCC CTCGGTGGCT CCTGGGGATC CTGGTCCCTC CCTGTGACTG 
     1861 TGGACCTGCC TGGAGATGCA GTGGCACTTG GACTGCAATG CTTTACCTTG GACCTGAAGA 
     1921 ATGTTACCTG TCAATGGCAG CAACAGGACC ATGCTAGCTC CCAAGGCTTC TTCTACCACA 
     1981 GCAGGGCACG GTGCTGCCCC AGAGACAGGT ACCCCATCTG GGAGAACTGC GAAGAGGAAG 
     2041 AGAAAACAAA TCCAGGACTA CAGACCCCAC AGTTCTCTCG CTGCCACTTC AAGTCACGAA 
     2101 ATGACAGCAT TATTCACATC CTTGTGGAGG TGACCACAGC CCCGGGTACT GTTCACAGCT 
     2161 ACCTGGGCTC CCCTTTCTGG ATCCACCAGG CTGTGCGCCT CCCCACCCCA AACTTGCACT 
     2221 GGAGGGAGAT CTCCAGTGGG CATCTGGAAT TGGAGTGGCA GCACCCATCG TCCTGGGCAG 
     2281 CCCAAGAGAC CTGTTATCAA CTCCGATACA CAGGAGAAGG CCATCAGGAC TGGAAGGTGC 
     2341 TGGAGCCGCC TCTCGGGGCC CGAGGAGGGA CCCTGGAGCT GCGCCCGCGA TCTCGCTACC 
     2401 GTTTACAGCT GCGCGCCAGG CTCAACGGCC CCACCTACCA AGGTCCCTGG AGCTCGTGGT 
     2461 CGGACCCAAC TAGGGTGGAG ACCGCCACCG AGACCGCCTG GATCTCCTTG GTGACCGCTC 
     2521 TGCATCTAGT GCTGGGCCTC AGCGCCGTCC TGGGCCTGCT GCTGCTGAGG TGGCAGTTTC 
     2581 CTGCACACTA CAGGAGACTG AGGCATGCCC TGTGGCCCTC ACTTCCAGAC CTGCACCGGG 
     2641 TCCTAGGCCA GTACCTTAGG GACACTGCAG CCCTGAGCCC GCCCAAGGCC ACAGTCTCAG 
     2701 ATACCTGTGA AGAAGTGGAA CCCAGCCTCC TTGAAATCCT CCCCAAGTCC TCAGAGAGGA 
     2761 CTCCTTTGCC CCTGTGTTCC TCCCAGGCCC AGATGGACTA CCGAAGATTG CAGCCTTCTT 
     2821 GCCTGGGGAC CATGCCCCTG TCTGTGTGCC CACCCATGGC TGAGTCAGGG TCCTGCTGTA 
     2881 CCACCCACAT TGCCAACCAT TCCTACCTAC CACTAAGCTA TTGGCAGCAG CCTACGCGTA 
     2941 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3001 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3061 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3121 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3181 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3241 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3301 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3361 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3421 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3481 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3541 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3601 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     3661 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     3721 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     3781 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     3841 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     3901 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     3961 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4021 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4081 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4141 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4201 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4261 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4321 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4381 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4441 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4501 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4561 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4621 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     4681 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     4741 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     4801 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     4861 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     4921 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     4981 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5041 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5101 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5161 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5221 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5281 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5341 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5401 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5461 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5521 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5581 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     5641 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     5701 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     5761 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     5821 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     5881 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     5941 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6001 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6061 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6121 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6181 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6241 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6301 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6361 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6421 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6481 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6541 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6601 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     6661 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     6721 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     6781 AATTTT
//