LOCUS       RC224812                9204 bp    DNA     circular  SYN 
DEFINITION  NCOA1 (Myc-DDK-tagged)-Human nuclear receptor coactivator 1 (NCOA1), transcript variant 1
ACCESSION   NM_003743
VERSION     NM_003743.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5361..5452 
                     /label="Myc-DDK"  
     misc_feature    5482..6067 
                     /label="PolyA signal"  
     misc_feature    5539..5558 
                     /label="Restriction Enzyme"  
     misc_feature    6343..7015 
                     /label="ColE1"  
     misc_feature    7445..8239 
                     /label="Kan/Neo"  
     misc_feature    8274..8576 
                     /label="SV40 ori"  
     misc_feature    8662..9139 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5343..5349 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5343 
                     /label="NCOA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGCCTC GGGGACAGTT CATCCGACCC TGCTAACCCA GACTCACATA 
     1081 AGAGGAAAGG ATCGCCATGT GACACACTGG CATCAAGCAC GGAAAAGAGG CGCAGGGAGC 
     1141 AAGAAAATAA ATATTTAGAA GAACTAGCTG AGTTACTGTC TGCCAACATT AGTGACATTG 
     1201 ACAGCTTGAG TGTAAAACCA GACAAATGCA AGATTTTGAA GAAAACAGTC GATCAGATAC 
     1261 AGCTAATGAA GAGAATGGAA CAAGAGAAAT CAACAACTGA TGACGATGTA CAGAAATCAG 
     1321 ACATCTCATC AAGTAGTCAA GGAGTGATAG AAAAGGAATC CTTGGGACCT CTTCTTTTGG 
     1381 AGGCTTTGGA TGGATTTTTC TTTGTTGTGA ACTGTGAAGG GAGAATTGTA TTTGTGTCAG 
     1441 AGAATGTAAC CAGCTACTTA GGTTACAATC AGGAGGAATT AATGAATACC AGCGTCTACA 
     1501 GCATACTGCA CGTGGGGGAT CATGCAGAAT TTGTGAAGAA TCTGCTACCA AAATCACTAG 
     1561 TAAATGGAGT TCCTTGGCCT CAAGAGGCAA CACGACGAAA TAGCCATACC TTTAACTGCA 
     1621 GGATGCTAAT TCACCCTCCA GATGAGCCAG GGACCGAGAA CCAAGAAGCT TGCCAGCGTT 
     1681 ATGAAGTAAT GCAGTGTTTC ACTGTGTCAC AGCCAAAATC AATTCAAGAG GATGGAGAAG 
     1741 ATTTCCAGTC ATGTCTGATT TGTATTGCAC GGCGATTACC TCGGCCTCCA GCTATTACGG 
     1801 GTGTAGAATC CTTTATGACC AAGCAAGATA CTACAGGTAA AATCATCTCT ATTGATACTA 
     1861 GTTCCCTGAG AGCTGCTGGC AGAACTGGTT GGGAAGATTT AGTGAGGAAG TGCATTTATG 
     1921 CTTTTTTCCA ACCTCAGGGC AGAGAACCAT CTTATGCCAG ACAGCTGTTC CAAGAAGTGA 
     1981 TGACTCGTGG CACTGCCTCC AGCCCCTCCT ATAGATTCAT ATTGAATGAT GGGACAATGC 
     2041 TTAGCGCCCA CACCAAGTGT AAACTTTGCT ACCCTCAAAG TCCAGACATG CAACCTTTCA 
     2101 TCATGGGAAT TCATATCATC GACAGGGAGC ACAGTGGGCT TTCTCCTCAA GATGACACTA 
     2161 ATTCTGGAAT GTCAATTCCC CGAGTAAATC CCTCGGTCAA TCCTAGTATC TCTCCAGCTC 
     2221 ATGGTGTGGC TCGTTCATCC ACATTGCCAC CATCCAACAG CAACATGGTA TCCACCAGAA 
     2281 TAAACCGCCA GCAGAGCTCA GACCTTCATA GCAGCAGTCA TAGTAATTCT AGCAACAGCC 
     2341 AAGGAAGTTT CGGATGCTCA CCCGGAAGTC AGATTGTAGC CAATGTTGCC TTAAACCAAG 
     2401 GACAGGCCAG TTCACAGAGC AGTAATCCCT CTTTAAACCT CAATAATTCT CCTATGGAAG 
     2461 GTACAGGAAT ATCCCTAGCA CAGTTCATGT CTCCAAGGAG ACAGGTTACT TCTGGATTGG 
     2521 CAACAAGGCC CAGGATGCCA AACAATTCCT TTCCTCCTAA TATTTCGACA TTAAGCTCTC 
     2581 CCGTTGGCAT GACAAGTAGT GCCTGTAATA ATAATAACCG ATCTTATTCA AACATCCCAG 
     2641 TAACATCTTT ACAGGGTATG AATGAAGGAC CCAATAACTC CGTTGGCTTC TCTGCCAGTT 
     2701 CTCCAGTCCT CAGGCAGATG AGCTCACAGA ATTCACCTAG CAGATTAAAT ATACAACCAG 
     2761 CAAAAGCTGA GTCCAAAGAT AACAAAGAGA TTGCCTCAAT TTTAAATGAA ATGATTCAAT 
     2821 CTGACAACAG CTCTAGTGAT GGCAAACCTC TGGATTCAGG GCTTCTGCAT AACAATGACA 
     2881 GACTTTCAGA TGGAGACAGT AAATACTCTC AAACCAGTCA CAAACTAGTG CAGCTTTTGA 
     2941 CAACAACTGC CGAACAGCAG TTACGGCATG CTGATATAGA CACAAGCTGC AAAGATGTCC 
     3001 TGTCTTGCAC AGGCACTTCC AACTCTGCCT CTGCTAACTC TTCAGGAGGT TCTTGTCCCT 
     3061 CTTCTCATAG CTCATTGACA GAACGGCATA AAATTCTACA CCGGCTCTTA CAGGAGGGTA 
     3121 GCCCCTCAGA TATCACCACT TTGTCTGTCG AGCCTGATAA AAAGGACAGT GCATCTACTT 
     3181 CTGTGTCAGT GACTGGACAG GTACAAGGAA ACTCCAGTAT AAAACTAGAA CTGGATGCTT 
     3241 CAAAGAAAAA AGAATCAAAA GACCATCAGC TCCTACGCTA TCTTTTAGAT AAAGATGAGA 
     3301 AAGATTTAAG ATCAACTCCA AACCTGAGCC TGGATGATGT AAAGGTGAAA GTGGAAAAGA 
     3361 AAGAACAGAT GGATCCATGT AATACAAACC CAACCCCAAT GACCAAACCC ACTCCTGAGG 
     3421 AAATAAAACT GGAGGCCCAG AGCCAGTTTA CAGCTGACCT TGACCAGTTT GATCAGTTAC 
     3481 TGCCCACGCT GGAGAAGGCA GCACAGTTGC CAGGCTTATG TGAGACAGAC AGGATGGATG 
     3541 GTGCGGTCAC CAGTGTAACC ATCAAATCGG AGATCCTGCC AGCTTCACTT CAGTCCGCCA 
     3601 CTGCCAGACC CACTTCCAGG CTAAATAGAT TACCTGAGCT GGAATTGGAA GCAATTGATA 
     3661 ACCAATTTGG ACAACCAGGA ACAGGCGATC AGATTCCATG GACAAATAAT ACAGTGACAG 
     3721 CTATAAATCA GAGTAAATCA GAAGACCAGT GTATTAGCTC ACAATTAGAT GAGCTTCTCT 
     3781 GTCCACCCAC AACAGTAGAA GGGAGAAATG ATGAGAAGGC TCTTCTTGAA CAGCTGGTAT 
     3841 CCTTCCTTAG TGGCAAAGAT GAAACTGAGC TAGCTGAACT AGACAGAGCT CTGGGAATTG 
     3901 ACAAACTTGT TCAGGGGGGT GGATTAGATG TATTATCAGA GAGATTTCCA CCACAACAAG 
     3961 CAACGCCACC TTTGATCATG GAAGAAAGAC CCAACCTTTA TTCCCAGCCT TACTCTTCTC 
     4021 CTTCTCCTAC TGCCAATCTC CCTAGCCCTT TCCAAGGCAT GGTCAGGCAA AAACCTTCAC 
     4081 TGGGGACGAT GCCTGTTCAA GTAACACCTC CCCGAGGTGC TTTTTCACCT GGCATGGGCA 
     4141 TGCAGCCCAG GCAAACTCTA AACAGACCTC CGGCTGCACC TAACCAGCTT CGACTTCAAC 
     4201 TACAGCAGCG ATTACAGGGA CAACAGCAGT TGATACACCA AAATCGGCAA GCTATCTTAA 
     4261 ACCAGTTTGC AGCAACTGCT CCTGTTGGCA TCAATATGAG ATCAGGCATG CAACAGCAAA 
     4321 TTACACCTCA GCCACCCCTG AATGCTCAAA TGTTGGCACA ACGTCAGCGG GAACTGTACA 
     4381 GTCAACAGCA CCGACAGAGG CAGCTAATAC AGCAGCAAAG AGCCATGCTT ATGAGGCAGC 
     4441 AAAGCTTTGG GAACAACCTC CCTCCCTCAT CTGGACTACC AGTTCAAATG GGGAACCCCC 
     4501 GTCTTCCTCA GGGTGCTCCA CAGCAATTCC CCTATCCACC AAACTATGGT ACAAATCCAG 
     4561 GAACCCCACC TGCTTCTACC AGCCCGTTTT CACAACTAGC AGCAAATCCT GAAGCATCCT 
     4621 TGGCCAACCG CAACAGCATG GTGAGCAGAG GCATGACAGG AAACATAGGA GGACAGTTTG 
     4681 GCACTGGAAT CAATCCTCAG ATGCAGCAGA ATGTCTTCCA GTATCCAGGA GCAGGAATGG 
     4741 TTCCCCAAGG TGAGGCCAAC TTTGCTCCAT CTCTAAGCCC TGGGAGCTCC ATGGTGCCGA 
     4801 TGCCAATCCC TCCTCCTCAG AGTTCTCTGC TCCAGCAAAC TCCACCTGCC TCCGGGTATC 
     4861 AGTCACCAGA CATGAAGGCC TGGCAGCAAG GAGCGATAGG AAACAACAAT GTGTTCAGTC 
     4921 AAGCTGTCCA GAACCAGCCC ACGCCTGCAC AGCCAGGAGT ATACAACAAC ATGAGCATCA 
     4981 CCGTTTCCAT GGCAGGTGGA AATACGAATG TTCAGAACAT GAACCCAATG ATGGCCCAGA 
     5041 TGCAGATGAG CTCTTTGCAG ATGCCAGGAA TGAACACTGT GTGCCCTGAG CAGATAAATG 
     5101 ATCCCGCACT GAGACACACA GGCCTCTACT GCAACCAGCT CTCATCCACT GACCTTCTCA 
     5161 AAACAGAAGC AGATGGAACC CAGCAGGTGC AACAGGTTCA GGTGTTTGCT GACGTCCAGT 
     5221 GTACAGTGAA TCTGGTAGGC GGGGACCCTT ACCTGAACCA GCCTGGTCCA CTGGGAACTC 
     5281 AAAAGCCCAC GTCAGGACCA CAGACCCCCC AGGCCCAGCA GAAGAGCCTC CTTCAGCAGC 
     5341 TACTGACTGA AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5401 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5461 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5521 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5581 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5641 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5701 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5761 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5821 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5881 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5941 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     6001 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     6061 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6121 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6181 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6241 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6301 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6361 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6421 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6481 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6541 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6601 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6661 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6721 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6781 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6841 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6901 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6961 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     7021 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     7081 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7141 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7201 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7261 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7321 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7381 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7441 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7501 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7561 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7621 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7681 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7741 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7801 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7861 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7921 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7981 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     8041 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     8101 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8161 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8221 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8281 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8341 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8401 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8461 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8521 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8581 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8641 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8701 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8761 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8821 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8881 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8941 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     9001 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     9061 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9121 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9181 TAACAAAAAT TTAACGCGAA TTTT
//