LOCUS       RC224806                7416 bp    DNA     circular  SYN 
DEFINITION  VAV1 (Myc-DDK-tagged)-Human vav 1 guanine nucleotide exchange factor (VAV1)
ACCESSION   NM_005428
VERSION     NM_005428.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3573..3664 
                     /label="Myc-DDK"  
     misc_feature    3694..4279 
                     /label="PolyA signal"  
     misc_feature    3751..3770 
                     /label="Restriction Enzyme"  
     misc_feature    4555..5227 
                     /label="ColE1"  
     misc_feature    5657..6451 
                     /label="Kan/Neo"  
     misc_feature    6486..6788 
                     /label="SV40 ori"  
     misc_feature    6874..7351 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3555..3561 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3555 
                     /label="VAV1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTGTGG CGCCAATGCA CCCACTGGCT CATCCAGTGC CGGGTGCTGC 
     1081 CGCCCAGCCA CCGCGTGACC TGGGATGGGG CTCAGGTGTG TGAACTGGCC CAGGCCCTCC 
     1141 GGGATGGTGT CCTTCTGTGT CAGCTGCTTA ACAACCTGCT ACCCCATGCC ATCAACCTGC 
     1201 GTGAGGTCAA CCTGCGCCCC CAGATGTCCC AGTTCCTGTG CCTTAAGAAC ATTAGAACCT 
     1261 TCCTGTCCAC CTGCTGTGAG AAGTTCGGCC TCAAGCGGAG CGAGCTCTTC GAAGCCTTTG 
     1321 ACCTCTTCGA TGTGCAGGAT TTTGGCAAGG TCATCTACAC CCTGTCTGCT CTGTCCTGGA 
     1381 CCCCGATCGC CCAGAACAGG GGGATCATGC CCTTCCCCAC CGAGGAGGAG AGTGTAGGTG 
     1441 ATGAAGACAT CTACAGTGGC CTGTCCGACC AGATCGACGA CACGGTGGAG GAGGATGAGG 
     1501 ACCTGTATGA CTGCGTGGAG AATGAGGAGG CGGAAGGCGA CGAGATCTAT GAGGACCTCA 
     1561 TGCGCTCGGA GCCCGTGTCC ATGCCGCCCA AGATGACAGA GTATGACAAG CGCTGCTGCT 
     1621 GCCTGCGGGA GATCCAGCAG ACGGAGGAGA AGTACACTGA CACGCTGGGC TCCATCCAGC 
     1681 AGCATTTCTT GAAGCCCCTG CAACGGTTCC TGAAACCTCA AGACATTGAG ATCATCTTTA 
     1741 TCAACATTGA GGACCTGCTT CGTGTTCATA CTCACTTCCT AAAGGAGATG AAGGAAGCCC 
     1801 TGGGCACCCC TGGCGCAGCC AATCTCTACC AGGTCTTCAT CAAATACAAG GAGAGGTTCC 
     1861 TCGTCTATGG CCGCTACTGC AGCCAGGTGG AGTCAGCCAG CAAACACCTG GACCGTGTGG 
     1921 CCGCAGCCCG GGAGGACGTG CAGATGAAGC TGGAGGAATG TTCTCAGAGA GCCAACAACG 
     1981 GGAGGTTCAC CCTGCGGGAC CTGCTGATGG TGCCTATGCA GCGAGTTCTC AAATATCACC 
     2041 TCCTTCTCCA GGAGCTGGTG AAACACACGC AGGAGGCGAT GGAGAAGGAG AACCTGCGGC 
     2101 TGGCCCTGGA TGCCATGAGG GACCTGGCTC AGTGCGTGAA CGAGGTCAAG CGAGACAACG 
     2161 AGACACTGCG ACAGATCACC AATTTCCAGC TGTCCATTGA GAACCTGGAC CAGTCTCTGG 
     2221 CTCACTATGG CCGGCCCAAG ATCGACGGGG AACTCAAGAT CACCTCGGTG GAACGGCGCT 
     2281 CCAAGATGGA CAGGTATGCC TTCCTGCTCG ACAAAGCTCT ACTCATCTGT AAGCGCAGGG 
     2341 GAGACTCCTA TGACCTCAAG GACTTTGTAA ACCTGCACAG CTTCCAGGTT CGGGATGACT 
     2401 CTTCAGGAGA CCGAGACAAC AAGAAGTGGA GCCACATGTT CCTCCTGATC GAGGACCAAG 
     2461 GTGCCCAGGG CTATGAGCTG TTCTTCAAGA CAAGAGAATT GAAGAAGAAG TGGATGGAGC 
     2521 AGTTTGAGAT GGCCATCTCC AACATCTATC CGGAGAATGC CACCGCCAAC GGGCATGACT 
     2581 TCCAGATGTT CTCCTTTGAG GAGACCACAT CCTGCAAGGC CTGTCAGATG CTGCTTAGAG 
     2641 GTACCTTCTA TCAGGGCTAC CGCTGCCATC GGTGCCGGGC ATCTGCACAC AAGGAGTGTC 
     2701 TGGGGAGGGT CCCTCCATGT GGCCGACATG GGCAAGATTT CCCAGGAACT ATGAAGAAGG 
     2761 ACAAACTACA TCGCAGGGCT CAGGACAAAA AGAGGAATGA GCTGGGTCTG CCCAAGATGG 
     2821 AGGTGTTTCA GGAATACTAC GGGCTTCCTC CACCCCCTGG AGCCATTGGA CCCTTTCTAC 
     2881 GGCTCAACCC TGGAGACATT GTGGAGCTCA CGAAGGCTGA GGCTGAACAG AACTGGTGGG 
     2941 AGGGCAGAAA TACATCTACT AATGAAATTG GCTGGTTTCC TTGTAACAGG GTGAAGCCCT 
     3001 ATGTCCATGG CCCTCCTCAG GACCTGTCTG TTCATCTCTG GTACGCAGGC CCCATGGAGC 
     3061 GGGCAGGGGC AGAGAGCATC CTGGCCAACC GCTCGGACGG GACTTTCTTG GTGCGGCAGA 
     3121 GGGTGAAGGA TGCAGCAGAA TTTGCCATCA GCATTAAATA TAACGTCGAG GTCAAGCACA 
     3181 TTAAAATCAT GACAGCAGAA GGACTGTACC GGATCACAGA GAAAAAGGCT TTCCGGGGGC 
     3241 TTACGGAGCT GGTGGAGTTT TACCAGCAGA ACTCTCTAAA GGATTGCTTC AAGTCTCTGG 
     3301 ACACCACCTT GCAGTTCCCC TTCAAGGAGC CTGAAAAGAG AACCATCAGC AGGCCAGCAG 
     3361 TGGGAAGCAC AAAGTATTTT GGCACAGCCA AAGCCCGCTA TGACTTCTGC GCCCGAGACC 
     3421 GATCAGAGCT GTCGCTCAAG GAGGGTGACA TCATCAAGAT CCTTAACAAG AAGGGACAGC 
     3481 AAGGCTGGTG GCGAGGGGAG ATCTATGGCC GGGTTGGCTG GTTCCCTGCC AACTACGTGG 
     3541 AGGAAGATTA TTCTGAATAC TGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3601 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3661 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3721 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3781 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3841 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3901 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3961 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4021 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4081 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4141 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4201 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4261 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4321 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4381 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4441 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4501 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4561 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4621 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4681 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4741 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4801 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4861 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4921 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4981 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5041 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5101 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5161 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5221 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5281 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5341 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5401 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5461 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5521 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5581 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5641 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5701 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5761 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5821 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5881 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5941 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6001 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6061 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6121 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6181 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6241 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6301 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6361 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6421 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6481 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6541 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6601 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6661 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6721 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6781 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6841 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6901 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6961 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7021 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7081 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7141 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7201 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7261 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7321 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7381 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//