LOCUS       RC224799                8640 bp    DNA     circular  SYN 
DEFINITION  L1CAM (Myc-DDK-tagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 2
ACCESSION   NM_024003
VERSION     NM_024003.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4797..4888 
                     /label="Myc-DDK"  
     misc_feature    4918..5503 
                     /label="PolyA signal"  
     misc_feature    4975..4994 
                     /label="Restriction Enzyme"  
     misc_feature    5779..6451 
                     /label="ColE1"  
     misc_feature    6881..7675 
                     /label="Kan/Neo"  
     misc_feature    7710..8012 
                     /label="SV40 ori"  
     misc_feature    8098..8575 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4779..4785 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4779 
                     /label="L1CAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCGTGGCG CTGCGGTACG TGTGGCCTCT CCTCCTCTGC AGCCCCTGCC 
     1081 TGCTTATCCA GATCCCCGAG GAATATGAAG GACACCATGT GATGGAGCCA CCTGTCATCA 
     1141 CGGAACAGTC TCCACGGCGC CTGGTTGTCT TCCCCACAGA TGACATCAGC CTCAAGTGTG 
     1201 AGGCCAGTGG CAAGCCCGAA GTGCAGTTCC GCTGGACGAG GGATGGTGTC CACTTCAAAC 
     1261 CCAAGGAAGA GCTGGGTGTG ACCGTGTACC AGTCGCCCCA CTCTGGCTCC TTCACCATCA 
     1321 CGGGCAACAA CAGCAACTTT GCTCAGAGGT TCCAGGGCAT CTACCGCTGC TTTGCCAGCA 
     1381 ATAAGCTGGG CACCGCCATG TCCCATGAGA TCCGGCTCAT GGCCGAGGGT GCCCCCAAGT 
     1441 GGCCAAAGGA GACAGTGAAG CCCGTGGAGG TGGAGGAAGG GGAGTCAGTG GTTCTGCCTT 
     1501 GCAACCCTCC CCCAAGTGCA GAGCCTCTCC GGATCTACTG GATGAACAGC AAGATCTTGC 
     1561 ACATCAAGCA GGACGAGCGG GTGACGATGG GCCAGAACGG CAACCTCTAC TTTGCCAATG 
     1621 TGCTCACCTC CGACAACCAC TCAGACTACA TCTGCCACGC CCACTTCCCA GGCACCAGGA 
     1681 CCATCATTCA GAAGGAACCC ATTGACCTCC GGGTCAAGGC CACCAACAGC ATGATTGACA 
     1741 GGAAGCCGCG CCTGCTCTTC CCCACCAACT CCAGCAGCCA CCTGGTGGCC TTGCAGGGGC 
     1801 AGCCATTGGT CCTGGAGTGC ATCGCCGAGG GCTTTCCCAC GCCCACCATC AAATGGCTGC 
     1861 GCCCCAGTGG CCCCATGCCA GCCGACCGTG TCACCTACCA GAACCACAAC AAGACCCTGC 
     1921 AGCTGCTGAA AGTGGGCGAG GAGGATGATG GCGAGTACCG CTGCCTGGCC GAGAACTCAC 
     1981 TGGGCAGTGC CCGGCATGCG TACTATGTCA CCGTGGAGGC TGCCCCGTAC TGGCTGCACA 
     2041 AGCCCCAGAG CCATCTATAT GGGCCAGGAG AGACTGCCCG CCTGGACTGC CAAGTCCAGG 
     2101 GCAGGCCCCA ACCAGAGGTC ACCTGGAGAA TCAACGGGAT CCCTGTGGAG GAGCTGGCCA 
     2161 AAGACCAGAA GTACCGGATT CAGCGTGGCG CCCTGATCCT GAGCAACGTG CAGCCCAGTG 
     2221 ACACAATGGT GACCCAATGT GAGGCCCGCA ACCGGCACGG GCTCTTGCTG GCCAATGCCT 
     2281 ACATCTACGT TGTCCAGCTG CCAGCCAAGA TCCTGACTGC GGACAATCAG ACGTACATGG 
     2341 CTGTCCAGGG CAGCACTGCC TACCTTCTGT GCAAGGCCTT CGGAGCGCCT GTGCCCAGTG 
     2401 TTCAGTGGCT GGACGAGGAT GGGACAACAG TGCTTCAGGA CGAACGCTTC TTCCCCTATG 
     2461 CCAATGGGAC CCTGGGCATT CGAGACCTCC AGGCCAATGA CACCGGACGC TACTTCTGCC 
     2521 TGGCTGCCAA TGACCAAAAC AATGTTACCA TCATGGCTAA CCTGAAGGTT AAAGATGCAA 
     2581 CTCAGATCAC TCAGGGGCCC CGCAGCACAA TCGAGAAGAA AGGTTCCAGG GTGACCTTCA 
     2641 CGTGCCAGGC CTCCTTTGAC CCCTCCTTGC AGCCCAGCAT CACCTGGCGT GGGGACGGTC 
     2701 GAGACCTCCA GGAGCTTGGG GACAGTGACA AGTACTTCAT AGAGGATGGG CGCCTGGTCA 
     2761 TCCACAGCCT GGACTACAGC GACCAGGGCA ACTACAGCTG CGTGGCCAGT ACCGAACTGG 
     2821 ATGTGGTGGA GAGTAGGGCA CAGCTCTTGG TGGTGGGGAG CCCTGGGCCG GTGCCACGGC 
     2881 TGGTGCTGTC CGACCTGCAC CTGCTGACGC AGAGCCAGGT GCGCGTGTCC TGGAGTCCTG 
     2941 CAGAAGACCA CAATGCCCCC ATTGAGAAAT ATGACATTGA ATTTGAGGAC AAGGAAATGG 
     3001 CGCCTGAAAA ATGGTACAGT CTGGGCAAGG TTCCAGGGAA CCAGACCTCT ACCACCCTCA 
     3061 AGCTGTCGCC CTATGTCCAC TACACCTTTA GGGTTACTGC CATAAACAAA TATGGCCCCG 
     3121 GGGAGCCCAG CCCGGTCTCT GAGACTGTGG TCACACCTGA GGCAGCCCCA GAGAAGAACC 
     3181 CTGTGGATGT GAAGGGGGAA GGAAATGAGA CCACCAATAT GGTCATCACG TGGAAGCCGC 
     3241 TCCGGTGGAT GGACTGGAAC GCCCCCCAGG TTCAGTACCG CGTGCAGTGG CGCCCTCAGG 
     3301 GGACACGAGG GCCCTGGCAG GAGCAGATTG TCAGCGACCC CTTCCTGGTG GTGTCCAACA 
     3361 CGTCCACCTT CGTGCCCTAT GAGATCAAAG TCCAGGCCGT CAACAGCCAG GGCAAGGGAC 
     3421 CAGAGCCCCA GGTCACTATC GGCTACTCTG GAGAGGACTA CCCCCAGGCA ATCCCTGAGC 
     3481 TGGAAGGCAT TGAAATCCTC AACTCAAGTG CCGTGCTGGT CAAGTGGCGG CCGGTGGACC 
     3541 TGGCCCAGGT CAAGGGCCAC CTCCGCGGAT ACAATGTGAC GTACTGGAGG GAGGGCAGTC 
     3601 AGAGGAAGCA CAGCAAGAGA CATATCCACA AAGACCATGT GGTGGTGCCC GCCAACACCA 
     3661 CCAGTGTCAT CCTCAGTGGC TTGCGGCCCT ATAGCTCCTA CCACCTGGAG GTGCAGGCCT 
     3721 TTAACGGGCG AGGATCGGGG CCCGCCAGCG AGTTCACCTT CAGCACCCCA GAGGGAGTGC 
     3781 CTGGCCACCC CGAGGCGTTG CACCTGGAGT GCCAGTCGAA CACCAGCCTG CTGCTGCGCT 
     3841 GGCAGCCCCC ACTCAGCCAC AACGGCGTGC TCACCGGCTA CGTGCTCTCC TACCACCCCC 
     3901 TGGATGAGGG GGGCAAGGGG CAACTGTCCT TCAACCTTCG GGACCCCGAA CTTCGGACAC 
     3961 ACAACCTGAC CGATCTCAGC CCCCACCTGC GGTACCGCTT CCAGCTTCAG GCCACCACCA 
     4021 AAGAGGGCCC TGGTGAAGCC ATCGTACGGG AAGGAGGCAC TATGGCCTTG TCTGGGATCT 
     4081 CAGATTTTGG CAACATCTCA GCCACAGCGG GTGAAAACTA CAGTGTCGTC TCCTGGGTCC 
     4141 CCAAGGAGGG CCAGTGCAAC TTCAGGTTCC ATATCTTGTT CAAAGCCTTG GGAGAAGAGA 
     4201 AGGGTGGGGC TTCCCTTTCG CCACAGTATG TCAGCTACAA CCAGAGCTCC TACACGCAGT 
     4261 GGGACCTGCA GCCTGACACT GACTACGAGA TCCACTTGTT TAAGGAGAGG ATGTTCCGGC 
     4321 ACCAAATGGC TGTGAAGACC AATGGCACAG GCCGCGTGAG GCTCCCTCCT GCTGGCTTCG 
     4381 CCACTGAGGG CTGGTTCATC GGCTTTGTGA GTGCCATCAT CCTCCTGCTC CTCGTCCTGC 
     4441 TCATCCTCTG CTTCATCAAG CGCAGCAAGG GCGGCAAATA CTCAGTGAAG GATAAGGAGG 
     4501 ACACCCAGGT GGACTCTGAG GCCCGACCGA TGAAAGATGA GACCTTCGGC GAGTACAGTG 
     4561 ACAACGAGGA GAAGGCCTTT GGCAGCAGCC AGCCATCGCT CAACGGGGAC ATCAAGCCCC 
     4621 TGGGCAGTGA CGACAGCCTG GCCGATTATG GGGGCAGCGT GGATGTTCAG TTCAACGAGG 
     4681 ATGGTTCGTT CATTGGCCAG TACAGTGGCA AGAAGGAGAA GGAGGCGGCA GGGGGCAATG 
     4741 ACAGCTCAGG GGCCACTTCC CCCATCAACC CTGCCGTGGC CCTAGAAACG CGTACGCGGC 
     4801 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4861 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4921 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4981 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5041 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5101 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5161 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5221 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5281 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5341 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5401 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5461 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5521 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5581 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5641 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5701 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5761 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5821 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5881 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5941 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6001 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6061 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6121 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6181 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6241 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6301 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6361 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6421 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6481 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6541 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6601 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6661 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6721 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6781 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6841 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6901 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6961 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7021 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7081 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7141 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7201 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7261 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7321 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7381 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7441 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7501 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7561 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7621 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7681 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7741 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7801 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7861 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7921 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7981 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8041 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8101 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8161 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8221 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8281 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8341 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8401 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8461 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8521 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8581 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//