LOCUS       RC224789                9381 bp    DNA     circular  SYN 
DEFINITION  ATP7A (Myc-DDK-tagged)-Human ATPase, Cu++ transporting, alpha polypeptide (ATP7A). Note: ORF is codon optimized
ACCESSION   NM_000052
VERSION     NM_000052.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5538..5629 
                     /label="Myc-DDK"  
     misc_feature    5659..6244 
                     /label="PolyA signal"  
     misc_feature    5716..5735 
                     /label="Restriction Enzyme"  
     misc_feature    6520..7192 
                     /label="ColE1"  
     misc_feature    7622..8416 
                     /label="Kan/Neo"  
     misc_feature    8451..8753 
                     /label="SV40 ori"  
     misc_feature    8839..9316 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5520..5526 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5520 
                     /label="ATP7A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCCAAGT ATGGGTGTGA ATTCTGTTAC CATTTCTGTT GAGGGTATGA 
     1081 CTTGCAATTC CTGTGTTTGG ACCATTGAGC AGCAGATTGG AAAAGTGAAT GGTGTGCATC 
     1141 ACATTAAGGT ATCACTGGAA GAAAAAAATG CAACTATTAT TTATGACCCT AAACTACAGA 
     1201 CTCCAAAGAC CCTACAGGAA GCTATTGATG ACATGGGCTT TGATGCTGTT ATCCATAATC 
     1261 CTGACCCTCT CCCTGTTTTA ACTGACACCT TGTTTCTGAC TGTTACGGCG TCACTGACTT 
     1321 TGCCATGGGA CCATATCCAA AGCACATTGC TGAAGACCAA GGGTGTGACA GACATTAAAA 
     1381 TTTACCCTCA GAAAAGAACT GTAGCAGTGA CAATAATCCC TTCTATAGTG AATGCCAATC 
     1441 AGATAAAAGA GCTGGTTCCA GAACTCAGTT TAGATACTGG GACACTGGAG AAAAAGTCAG 
     1501 GAGCTTGTGA AGATCATAGT ATGGCTCAAG CTGGTGAAGT CGTGCTGAAG ATGAAAGTGG 
     1561 AAGGGATGAC CTGCCATTCA TGTACTAGCA CTATTGAAGG AAAAATTGGG AAACTGCAAG 
     1621 GTGTTCAGCG AATTAAAGTC TCCCTGGACA ATCAAGAAGC TACTATTGTT TATCAACCTC 
     1681 ATCTTATCTC AGTAGAGGAA ATGAAAAAGC AGATTGAAGC TATGGGCTTT CCAGCATTTG 
     1741 TCAAAAAGCA GCCCAAGTAC CTCAAATTGG GAGCTATTGA TGTAGAACGT CTAAAGAACA 
     1801 CACCAGTTAA ATCCTCAGAA GGGTCACAGC AAAGGAGTCC ATCATATACC AATGATTCAA 
     1861 CAGCCACTTT CATCATTGAT GGCATGCATT GTAAATCATG TGTGTCAAAT ATTGAAAGTA 
     1921 CTTTATCTGC ACTCCAATAT GTAAGCAGCA TAGTAGTTTC TTTAGAGAAT AGGTCTGCCA 
     1981 TTGTGAAGTA TAATGCAAGC TCAGTCACTC CAGAATCCCT GAGAAAAGCA ATAGAGGCTG 
     2041 TATCACCGGG GCTATATAGA GTTAGTATCA CAAGTGAAGT TGAGAGTACC TCAAACTCTC 
     2101 CCTCCAGCTC ATCTCTTCAG AAGATTCCTT TGAATGTAGT TAGCCAGCCT CTGACACAAG 
     2161 AAACTGTGAT AAACATTGAT GGCATGACTT GTAATTCCTG TGTGCAGTCT ATTGAGGGTG 
     2221 TCATATCAAA AAAGCCAGGT GTAAAATCCA TACGAGTCTC CCTTGCAAAT AGCAATGGGA 
     2281 CTGTTGAGTA TGATCCTCTA CTAACCTCTC CAGAAACGTT GAGAGGAGCA ATAGAAGACA 
     2341 TGGGATTTGA TGCTACCTTG TCAGACACGA ATGAGCCGTT GGTAGTAATA GCTCAGCCTT 
     2401 CATCGGAAAT GCCGCTTTTG ACTTCAACTA ATGAATTTTA TACTAAAGGG ATGACACCAG 
     2461 TTCAAGACAA GGAGGAAGGA AAGAATTCAT CTAAGTGTTA CATACAGGTC ACTGGCATGA 
     2521 CTTGCGCTTC CTGTGTAGCA AACATTGAAC GGAATTTAAG GCGGGAAGAA GGAATATATT 
     2581 CTATACTTGT GGCCCTGATG GCTGGCAAGG CAGAAGTAAG GTATAATCCT GCTGTTATAC 
     2641 AACCCCCAAT GATAGCAGAG TTCATCCGAG AACTTGGATT TGGAGCCACT GTGATAGAAA 
     2701 ATGCTGATGA AGGAGATGGT GTTTTGGAAC TTGTTGTGAG GGGAATGACG TGTGCCTCCT 
     2761 GCGTACATAA AATAGAGTCT AGTCTCACAA AACACAGAGG GATCCTATAC TGCTCCGTGG 
     2821 CCCTGGCAAC CAACAAAGCA CATATTAAAT ATGACCCAGA AATTATTGGT CCTAGAGATA 
     2881 TTATCCATAC AATTGAAAGC TTAGGTTTTG AAGCTTCTTT GGTCAAGAAG GATCGGTCAG 
     2941 CAAGTCACTT AGATCATAAA CGAGAAATAA GACAATGGAG ACGGTCTTTT CTTGTGAGTC 
     3001 TGTTTTTCTG TATTCCTGTA ATGGGGCTGA TGATATATAT GATGGTTATG GACCACCACT 
     3061 TTGCAACTCT TCACCATAAT CAAAACATGA GTAAAGAAGA AATGATCAAC CTTCATTCTT 
     3121 CTATGTTCCT GGAGCGCCAG ATTCTTCCAG GATTGTCTGT TATGAATTTG CTGTCCTTTT 
     3181 TATTGTGTGT ACCTGTACAG TTTTTCGGAG GCTGGTACTT CTACATTCAG GCTTATAAAG 
     3241 CACTGAAGCA TAAGACAGCA AATATGGACG TACTGATTGT GCTGGCAACC ACCATTGCAT 
     3301 TTGCCTACTC TTTGATTATT CTTCTAGTTG CAATGTATGA GAGAGCCAAA GTGAACCCTA 
     3361 TTACTTTCTT TGACACACCC CCTATGCTGT TTGTGTTTAT TGCACTAGGC CGATGGCTGG 
     3421 AACATATAGC AAAGGGCAAA ACATCAGAGG CTCTTGCAAA GTTAATTTCA CTACAAGCTA 
     3481 CAGAAGCAAC TATTGTAACT CTTGATTCTG ATAATATCCT CCTCAGTGAA GAACAAGTGG 
     3541 ATGTGGAACT TGTACAACGT GGAGATATCA TTAAAGTAGT TCCAGGAGGC AAATTTCCAG 
     3601 TGGATGGTCG TGTTATTGAA GGACATTCTA TGGTAGATGA GTCCCTCATC ACAGGGGAGG 
     3661 CAATGCCTGT GGCTAAGAAA CCTGGCAGCA CAGTGATTGC TGGTTCCATT AACCAGAACG 
     3721 GGTCACTGCT TATCTGCGCA ACACATGTTG GAGCAGACAC AACCCTTTCT CAAATTGTCA 
     3781 AACTTGTGGA AGAGGCACAA ACATCAAAGG CTCCTATCCA GCAGTTTGCA GACAAACTCA 
     3841 GTGGCTATTT TGTTCCTTTT ATTGTTTTTG TTTCCATTGC CACCCTCTTG GTATGGATTG 
     3901 TAATTGGATT TCTGAATTTT GAAATTGTGG AAACCTACTT TCCTGGCTAC AATAGAAGTA 
     3961 TCTCCCGAAC AGAAACGATA ATACGATTTG CTTTCCAAGC CTCTATCACA GTTCTGTGTA 
     4021 TTGCATGTCC CTGTTCACTG GGACTGGCCA CTCCAACTGC TGTGATGGTG GGTACAGGAG 
     4081 TAGGTGCTCA AAATGGCATA CTAATAAAAG GTGGAGAGCC ATTGGAGATG GCTCATAAGG 
     4141 TAAAGGTAGT GGTATTTGAT AAGACTGGAA CCATTACTCA CGGAACCCCA GTGGTGAATC 
     4201 AAGTAAAGGT TCTAACTGAA AGTAACAGAA TATCACACCA TAAAATCTTG GCCATTGTGG 
     4261 GAACTGCTGA AAGTAACAGT GAACACCCTC TAGGAACAGC CATAACCAAA TATTGCAAAC 
     4321 AGGAGCTGGA CACTGAAACC TTGGGTACCT GCATAGATTT CCAGGTTGTG CCAGGCTGTG 
     4381 GTATTAGCTG TAAAGTCACC AATATTGAAG GCTTGCTACA TAAGAATAAC TGGAATATAG 
     4441 AGGACAATAA TATTAAAAAT GCATCCCTGG TTCAAATTGA TGCCAGTAAT GAACAGTCAT 
     4501 CAACTTCGTC TTCCATGATT ATTGATGCCC AGATCTCAAA TGCTCTTAAT GCTCAGCAGT 
     4561 ATAAAGTCCT CATTGGTAAC CGGGAGTGGA TGATTAGAAA TGGTCTTGTC ATTAATAACG 
     4621 ATGTAAATGA TTTCATGACT GAACATGAGA GAAAAGGTCG GACTGCTGTA TTAGTAGCAG 
     4681 TTGATGATGA GCTGTGTGGC TTGATAGCCA TTGCAGACAC AGTGAAGCCT GAAGCAGAAC 
     4741 TGGCTATCCA TATTCTGAAA TCTATGGGCT TAGAAGTAGT TCTGATGACT GGAGACAACA 
     4801 GTAAAACAGC TAGATCTATT GCTTCTCAGG TTGGCATTAC TAAGGTGTTT GCTGAAGTTC 
     4861 TACCTTCTCA CAAGGTTGCT AAAGTGAAGC AACTTCAAGA GGAGGGGAAA CGGGTAGCAA 
     4921 TGGTGGGAGA TGGAATCAAT GACTCCCCAG CTCTGGCAAT GGCTAATGTG GGAATTGCTA 
     4981 TTGGCACAGG CACAGATGTA GCCATTGAAG CAGCTGATGT GGTTTTGATA AGGAATGATC 
     5041 TTCTGGATGT AGTGGCAAGT ATTGACTTAT CAAGAGAGAC AGTCAAGAGG ATTCGGATAA 
     5101 ATTTTGTCTT TGCTCTAATT TATAATCTGG TTGGAATTCC CATAGCTGCT GGAGTTTTTA 
     5161 TGCCCATTGG TTTGGTTTTG CAGCCCTGGA TGGGATCTGC AGCAATGGCT GCTTCATCTG 
     5221 TTTCTGTAGT ACTTTCTTCT CTCTTCCTTA AACTTTACAG GAAACCAACT TACGAGAGTT 
     5281 ATGAACTGCC TGCCCGGAGC CAGATAGGAC AGAAGAGTCC TTCAGAAATC AGCGTTCATG 
     5341 TTGGAATAGA TGATACCTCA AGGAATTCTC CTAAACTGGG TTTGCTGGAC CGGATTGTTA 
     5401 ATTATAGCAG AGCCTCTATA AACTCACTAC TGTCTGATAA ACGCTCCCTA AACAGTGTTG 
     5461 TTACCAGTGA ACCTGACAAG CACTCACTCC TGGTGGGAGA CTTCAGGGAA GATGATGACA 
     5521 CTGCATTAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5581 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5641 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5701 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5761 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5821 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5881 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5941 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     6001 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     6061 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     6121 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     6181 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     6241 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6301 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6361 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6421 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6481 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6541 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6601 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6661 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6721 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6781 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6841 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6901 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6961 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     7021 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     7081 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     7141 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     7201 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     7261 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7321 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7381 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7441 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7501 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7561 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7621 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7681 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7741 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7801 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7861 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7921 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7981 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     8041 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     8101 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     8161 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     8221 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     8281 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8341 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8401 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8461 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8521 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8581 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8641 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8701 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8761 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8821 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8881 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8941 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     9001 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     9061 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     9121 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     9181 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     9241 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9301 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9361 CAAAAATTTA ACGCGAATTT T
//