LOCUS       RC224785                9513 bp    DNA     circular  SYN 
DEFINITION  ARHGEF12 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant 1
ACCESSION   NM_015313
VERSION     NM_015313.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5670..5761 
                     /label="Myc-DDK"  
     misc_feature    5791..6376 
                     /label="PolyA signal"  
     misc_feature    5848..5867 
                     /label="Restriction Enzyme"  
     misc_feature    6652..7324 
                     /label="ColE1"  
     misc_feature    7754..8548 
                     /label="Kan/Neo"  
     misc_feature    8583..8885 
                     /label="SV40 ori"  
     misc_feature    8971..9448 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5652..5658 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5652 
                     /label="ARHGEF12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGCACA CAGTCTACTA TCACCGACAG GTTTCCCCTC AAAAAACCTA 
     1081 TAAGGCATGG AAGTATTTTG AACCGAGAGT CACCAACAGA TAAGAAGCAG AAAGTTGAGC 
     1141 GCATTGCATC ACATGATTTT GACCCCACAG ATAGCTCCTC CAAGAAGACA AAGTCTAGTT 
     1201 CAGAGGAGAG TAGATCCGAG ATATATGGTC TTGTTCAGCG TTGCGTAATC ATCCAGAAAG 
     1261 ATGACAATGG ATTTGGGCTG ACGGTCAGTG GAGACAATCC AGTCTTCGTA CAGTCTGTCA 
     1321 AAGAAGATGG AGCAGCCATG CGGGCTGGAG TACAGACAGG TGATCGAATC ATCAAGGTGA 
     1381 ATGGAACTCT GGTGACTCAT TCAAATCATC TGGAGGTGGT GAAGCTAATC AAATCTGGTT 
     1441 CCTATGTAGC TCTCACTGTT CAGGGACGCC CACCTGGGTC GCCCCAGATT CCACTTGCCG 
     1501 ACTCTGAAGT AGAGCCGTCA GTCATTGGAC ATATGTCTCC CATCATGACA TCTCCTCATT 
     1561 CACCTGGAGC ATCTGGGAAT ATGGAGAGAA TCACTAGTCC TGTGCTCATG GGGGAGGAAA 
     1621 ACAATGTGGT TCATAACCAG AAAGTAGAAA TTCTGAGAAA AATGTTACAG AAAGAACAGG 
     1681 AACGGCTACA GTTATTGCAG GAAGATTACA ACCGAACACC TGCCCAAAGA TTGCTAAAAG 
     1741 AGATCCAAGA GGCCAAGAAA CACATTCCTC AGCTGCAAGA GCAGTTATCC AAAGCCACAG 
     1801 GCTCTGCTCA GGATGGAGCT GTAGTTACAC CCTCCAGACC TTTAGGGGAC ACCCTAACAG 
     1861 TCAGTGAGGC AGAAACAGAT CCTGGAGATG TACTGGGCAG GACTGACTGT AGCAGTGGAG 
     1921 ATGCTTCTCG GCCCAGTAGT GACAATGCAG ATAGTCCCAA GAGTGGCCCA AAAGAGAGAA 
     1981 TTTATCTAGA GGAAAACCCA GAGAAAAGTG AAACAATTCA GGACACTGAC ACTCAATCAC 
     2041 TTGTCGGAAG TCCCTCAACC CGTATAGCAC CTCATATTAT TGGAGCAGAA GATGATGATT 
     2101 TTGGTACTGA ACATGAACAG ATCAATGGAC AGTGCAGCTG TTTCCAGAGC ATTGAATTAC 
     2161 TAAAATCTCG CCCGGCTCAT TTGGCTGTTT TCTTACACCA TGTAGTTTCA CAATTTGACC 
     2221 CTGCGACTTT GCTCTGTTAT CTCTATTCAG ACCTGTATAA ACATACCAAT TCCAAAGAAA 
     2281 CTCGTCGCAT CTTCCTTGAG TTTCATCAGT TCTTTCTAGA TCGATCAGCA CACCTGAAAG 
     2341 TTTCTGTTCC TGATGAAATG TCTGCAGATC TAGAAAAGAG AAGACCTGAG CTCATTCCTG 
     2401 AGGATCTGCA TCGCCACTAT ATCCAAACTA TGCAAGAAAG AGTCCATCCA GAAGTTCAAA 
     2461 GGCACTTAGA AGATTTTCGG CAGAAACGTA GTATGGGACT GACCTTGGCT GAAAGCGAGC 
     2521 TGACTAAACT TGATGCAGAG CGAGACAAGG ACCGATTGAC TTTGGAGAAG GAGCGGACAT 
     2581 GTGCAGAACA GATTGTTGCC AAAATTGAAG AAGTATTGAT GACTGCTCAG GCTGTAGAGG 
     2641 AAGATAAGAG CTCCACCATG CAGTATGTTA TTCTCATGTA TATGAAGCAT TTGGGAGTAA 
     2701 AAGTGAAAGA GCCTCGAAAT TTGGAGCACA AACGGGGTCG GATTGGATTT CTTCCCAAAA 
     2761 TCAAGCAAAG TATGAAGAAA GATAAAGAAG GGGAAGAAAA AGGGAAGCGA AGAGGATTCC 
     2821 CCAGCATCCT GGGACCCCCA CGGAGACCAA GCCGTCATGA CAACAGTGCA ATTGGCAGAG 
     2881 CCATGGAACT ACAGAAGGCG CGCCACCCTA AGCACTTATC CACACCCTCA TCTGTGAGTC 
     2941 CTGAACCTCA GGACTCTGCC AAGTTGCGCC AGAGTGGGTT AGCAAATGAA GGAACAGACG 
     3001 CTGGATACCT GCCTGCCAAT TCCATGTCTT CTGTAGCTTC AGGGGCCTCT TTTTCCCAGG 
     3061 AAGGAGGGAA AGAGAATGAT ACAGGATCAA AGCAAGTTGG AGAAACATCA GCACCTGGAG 
     3121 ACACCTTAGA TGGCACACCT CGTACTCTCA ATACTGTCTT TGATTTCCCA CCACCTCCAT 
     3181 TAGACCAAGT GCAGGAGGAG GAATGTGAAG TAGAAAGGGT GACTGAACAT GGGACACCAA 
     3241 AGCCCTTTCG AAAGTTTGAC AGTGTAGCTT TTGGAGAAAG TCAAAGTGAG GATGAACAAT 
     3301 TTGAAAATGA CTTAGAGACA GACCCACCCA ACTGGCAGCA GCTTGTTAGT CGAGAAGTGT 
     3361 TACTGGGACT AAAACCTTGT GAAATCAAAA GACAGGAAGT GATTAATGAA TTGTTCTACA 
     3421 CTGAAAGAGC TCATGTTCGA ACACTGAAGG TTCTTGATCA AGTGTTCTAT CAGCGAGTAT 
     3481 CCAGAGAAGG AATTCTGTCA CCCTCAGAGC TACGGAAAAT TTTTTCAAAC TTGGAAGATA 
     3541 TTCTTCAACT TCATATTGGA TTGAATGAAC AAATGAAGGC TGTTCGAAAG AGAAATGAGA 
     3601 CCTCTGTTAT CGATCAGATT GGGGAAGATT TGCTGACATG GTTCAGCGGA CCAGGAGAGG 
     3661 AGAAATTGAA ACATGCTGCT GCTACCTTTT GCAGTAACCA ACCTTTCGCC CTGGAAATGA 
     3721 TCAAATCTCG TCAGAAAAAG GATTCTCGAT TTCAGACTTT TGTGCAAGAT GCTGAAAGTA 
     3781 ATCCACTGTG TCGTCGTCTT CAACTGAAGG ATATTATTCC CACTCAAATG CAAAGGCTTA 
     3841 CTAAGTACCC ACTTCTGTTG GATAATATTG CCAAATACAC AGAATGGCCA ACAGAAAGGG 
     3901 AGAAGGTGAA GAAAGCTGCA GATCACTGTC GTCAGATCTT AAATTATGTA AATCAGGCTG 
     3961 TCAAGGAGGC AGAAAACAAG CAGCGCCTAG AAGATTATCA GCGTCGCCTT GATACCTCCA 
     4021 GCCTGAAGTT GTCAGAGTAC CCAAATGTTG AAGAGCTCAG GAATTTGGAT TTAACAAAAA 
     4081 GGAAGATGAT TCATGAAGGG CCATTGGTTT GGAAGGTGAA TAGAGATAAA ACTATTGATT 
     4141 TATACACGTT GCTGCTGGAA GACATTCTTG TATTGTTACA AAAGCAGGAT GATAGACTGG 
     4201 TTTTAAGGTG TCATAGTAAG ATTCTGGCAT CTACAGCTGA TAGCAAACAC ACGTTTAGCC 
     4261 CTGTCATTAA GTTGAGTACA GTGTTGGTTC GACAAGTGGC AACAGATAAC AAAGCTTTAT 
     4321 TCGTCATTTC CATGTCAGAC AATGGCGCTC AGATTTATGA ACTGGTGGCA CAGACAGTTT 
     4381 CTGAAAAGAC TGTCTGGCAG GACCTAATCT GTCGGATGGC TGCATCAGTG AAGGAGCAAT 
     4441 CCACAAAGCC AATTCCATTA CCACAGTCAA CACCTGGCGA AGGAGATAAT GATGAAGAAG 
     4501 ATCCTTCAAA ATTAAAAGAG GAGCAGCATG GCATTTCAGT CACTGGTTTG CAGAGTCCAG 
     4561 ACAGAGATTT GGGATTAGAA TCTACCTTAA TATCGTCAAA ACCTCAGTCT CATTCACTGA 
     4621 GTACCTCTGG GAAATCAGAG GTACGTGATC TGTTTGTGGC TGAGAGACAG TTTGCAAAGG 
     4681 AACAACATAC AGATGGGACA CTAAAGGAAG TTGGAGAAGA TTATCAAATC GCAATCCCAG 
     4741 ATTCACACCT GCCTGTCTCA GAAGAACGGT GGGCATTGGA TGCACTAAGA AATTTGGGTT 
     4801 TGTTGAAGCA GTTGCTGGTG CAACAGCTAG GTTTGACTGA GAAGAGCGTT CAGGAAGACT 
     4861 GGCAACATTT CCCAAGATAC AGAACAGCCT CTCAGGGGCC GCAGACAGAC AGTGTCATCC 
     4921 AGAACTCTGA AAATATTAAG GCCTATCATT CTGGTGAAGG ACATATGCCC TTTAGAACTG 
     4981 GAACTGGTGA CATTGCAACT TGTTACAGTC CACGGACTTC AACTGAATCT TTTGCTCCAC 
     5041 GGGATTCAGT GGGACTGGCA CCCCAGGATA GCCAGGCAAG TAACATTTTA GTAATGGACC 
     5101 ACATGATTAT GACCCCAGAG ATGCCTACCA TGGAGCCAGA AGGGGGTCTT GATGACAGTG 
     5161 GAGAGCACTT TTTTGATGCC CGTGAAGCAC ATAGTGATGA GAATCCATCA GAAGGTGATG 
     5221 GAGCAGTTAA CAAGGAAGAG AAGGATGTTA ATTTACGCAT CTCAGGAAAC TATTTGATCC 
     5281 TTGATGGCTA TGACCCAGTG CAGGAGAGTT CCACAGATGA GGAGGTTGCT TCCTCACTTA 
     5341 CCCTGCAGCC CATGACAGGC ATCCCTGCTG TGGAATCCAC CCACCAGCAG CAACATTCTC 
     5401 CTCAGAATAC TCACTCCGAT GGGGCAATTT CACCATTCAC CCCCGAATTT CTGGTCCAGC 
     5461 AGCGCTGGGG AGCTATGGAG TATTCCTGTT TTGAGATCCA GAGTCCCTCC TCTTGTGCAG 
     5521 ATTCACAGAG CCAGATCATG GAGTACATTC ATAAGATAGA GGCTGACCTT GAACACTTAA 
     5581 AGAAGGTGGA GGAAAGTTAC ACCATTCTTT GCCAAAGGCT GGCTGGATCA GCCCTCACAG 
     5641 ACAAGCACTC AGATAAAAGT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5701 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5761 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5821 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5881 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5941 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     6001 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     6061 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     6121 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     6181 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6241 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6301 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6361 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6421 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6481 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6541 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6601 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6661 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6721 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6781 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6841 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6901 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6961 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     7021 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     7081 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     7141 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     7201 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7261 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7321 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7381 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7441 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7501 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7561 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7621 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7681 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7741 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7801 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7861 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7921 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7981 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     8041 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     8101 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     8161 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     8221 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8281 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8341 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8401 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8461 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8521 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8581 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8641 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8701 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8761 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8821 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8881 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8941 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     9001 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     9061 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     9121 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     9181 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9241 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9301 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9361 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9421 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9481 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//