LOCUS       RC224774L1                8931 bp    DNA     circular  SYN 
DEFINITION  MACC1 (Myc-DDK-tagged)-Human metastasis associated in colon cancer 1 (MACC1)
ACCESSION   NM_182762
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7222..7402 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8130..8789 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7561..8129 
                     /label="ColE1"  
     misc_feature    6097..6117 
                     /label="LR50 primer"  
     misc_feature    5894..5965 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6106..6697 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5876..5882 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5876 
                     /label="MACC1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCTAATCACT GAAAGAAAAC ATTTTCGGTC 
     3361 AGGAAGAATT GCACAAAGTA TGTCTGAAGC AAATTTGATT GACATGGAAG CTGGAAAACT 
     3421 CTCAAAAAGT TGCAATATTA CAGAATGCCA GGACCCAGAC TTGCTTCACA ATTGGCCGGA 
     3481 TGCTTTCACC CTTCGTGGTA ATAATGCTTC CAAAGTTGCA AATCCATTCT GGAATCAACT 
     3541 GTCTGCTTCT AACCCATTTT TGGATGACAT AACTCAACTA AGAAATAACA GGAAGAGAAA 
     3601 TAATATTTCC ATCTTAAAGG AAGATCCTTT TCTTTTCTGT AGAGAAATAG AAAATGGAAA 
     3661 TTCTTTTGAT TCCTCCGGTG ATGAACTTGA TGTGCATCAG TTACTTAGGC AGACTTCCTC 
     3721 AAGAAATTCT GGAAGATCTA AAAGTGTTTC AGAACTTCTG GACATTTTAG ACGACACAGC 
     3781 ACATGCCCAT CAGAGTATAC ATAACTCTGA CCAGATCCTA CTACACGACT TAGAGTGGCT 
     3841 TAAAAATGAT CGGGAGGCTT ATAAAATGGC TTGGTTAAGT CAACGCCAGC TGGCCCGCTC 
     3901 CTGCCTTGAT TTGAATACAA TTAGTCAGAG CCCTGGATGG GCCCAGACAC AACTTGCGGA 
     3961 GGTCACCATA GCTTGCAAAG TAAACCATCA AGGAGGGTCA GTACAATTAC CTGAATCAGA 
     4021 CATCACTGTT CATGTGCCCC AAGGTCATGT GGCTGTGGGA GAATTCCAAG AGGTGTCTCT 
     4081 AAGGGCTTTC CTTGATCCGC CACACATGCT TAACCATGAT CTTTCGTGCA CTGTGAGCCC 
     4141 GTTGTTGGAA ATCATGTTAG GCAACCTCAA TACAATGGAA GCCCTTTTGC TGGAGATGAA 
     4201 AATTGGGGCT GAAGTAAGAA AGGATCCTTT CAGCCAAGTC ATGACAGAAA TGGTGTGTTT 
     4261 ACACAGCTTG GGTAAAGAAG GCCCTTTTAA AGTTTTAAGC AACTGCTACA TTTATAAAGA 
     4321 CACCATCCAA GTCAAGCTAA TCGACTTGAG TCAGGTAATG TATCTAGTGG TTGCTGCACA 
     4381 AGCTAAAGCT CTTCCGTCAC CAGCTGCCAC CATTTGGGAT TATATCCACA AAACCACCTC 
     4441 AATTGGAATT TATGGACCCA AATATATCCA TCCCAGTTTT ACTGTTGTTT TAACAGTTTG 
     4501 TGGACACAAT TATATGCCAG GACAGCTTAC AATTTCTGAT ATTAAGAAGG GTGGAAAAAA 
     4561 CATATCTCCA GTTGTGTTTC AGCTCTGGGG GAAGCAGTCA TTTTTACTTG ACAAGCCACA 
     4621 AGATTTAAGT ATTTCTATTT TTTCCTGTGA TCCTGATTTT GAAGTAAAGA CAGAAGGAGA 
     4681 AAGGAAAGAA ATTAAACAAA AGCAGTTGGA AGCAGGTGAA GTAGTTCATC AACAATTTTT 
     4741 ATTTTCTTTA GTTGAGCACA GAGAGATGCA CTTGTTTGAT TTTTGTGTTC AAGTGGAGCC 
     4801 TCCCAATGGT GAACCAGTTG CACAGTTCTC TATCACTACT CCTGATCCAA CCCCAAACCT 
     4861 AAAAAGACTC TCGAATCTGC CAGGCTATTT GCAGAAGAAG GAGGAAATCA AGTCTGCTCC 
     4921 TTTATCACCA AAAATTCTTG TTAAATATCC TACATTTCAA GATAAAACAT TGAACTTTAG 
     4981 CAACTATGGG GTAACCCTGA AGGCAGTGCT AAGACAAAGC AAGATTGATT ACTTCCTTGA 
     5041 ATATTTCAAA GGGGACACAA TAGCTCTCCT CGGGGAAGGT AAGGTAAAAG CTATTGGTCA 
     5101 GTCCAAAGTG AAAGAATGGT ATGTAGGAGT CCTCAGAGGT AAGATTGGAC TTGTACACTG 
     5161 CAAAAATGTC AAGGTGATTT CAAAGGAGCA AGTAATGTTT ATGTCAGATA GTGTCTTTAC 
     5221 AACCAGAAAT CTTCTTGAAC AGATTGTCCT GCCTTTAAAA AAATTGACTT ATATCTACTC 
     5281 AGTTGTATTA ACCTTGGTGT CAGAAAAAGT TTATGATTGG AAAGTTTTAG CTGATGTCCT 
     5341 GGGTTACTCA CATCTGTCCC TGGAAGATTT TGATCAAATT CAAGCAGACA AAGAATCAGA 
     5401 GAAAGTTTCT TATGTTATAA AGAAGTTAAA GGAAGATTGC CACACAGAGA GAAATACAAG 
     5461 GAAGTTTCTG TATGAACTTA TTGTGGCTCT TCTGAAAATG GATTGCCAAG AGTTAGTCGC 
     5521 ACGTCTCATC CAAGAAGCTG CTGTTCTGAC TTCAGCTGTC AAGCTTGGAA AAGGCTGGAG 
     5581 GGAACTAGCT GAAAAGTTAG TACGACTCAC AAAGCAACAA ATGGAGGCAT ATGAAATTCC 
     5641 TCATCGAGGA AACACTGGAG ATGTTGCTGT TGAGATGATG TGGAAACCTG CCTATGATTT 
     5701 TCTGTATACC TGGAGTGCTC ACTATGGAAA TAACTACAGA GATGTGTTAC AAGACCTTCA 
     5761 GTCAGCTTTG GACAGAATGA AAAACCCTGT GACTAAACAC TGGAGAGAAT TAACTGGAGT 
     5821 TTTAATACTA GTAAATTCTT TGGAGGTTTT GAGAGTAACT GCATTCTCCA CTTCTGAGGA 
     5881 AGTAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     5941 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA 
     6001 CAAGTAGGAT TCGTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA 
     6061 CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA TCAAGCTTAT CGATAATCAA 
     6121 CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT 
     6181 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT 
     6241 TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC 
     6301 GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG 
     6361 GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC 
     6421 ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC 
     6481 ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT 
     6541 GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA 
     6601 GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT 
     6661 CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGACCT 
     6721 CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC 
     6781 TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG TCACACCTCA 
     6841 GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA 
     6901 AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC TTGATCTGTG 
     6961 GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG GGCCAGGGAT 
     7021 CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT 
     7081 AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC TGCATGGGAT 
     7141 GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA 
     7201 CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC 
     7261 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG 
     7321 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG 
     7381 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA GCAAAAGGCC AGCAAAAGGC 
     7441 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     7501 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     7561 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     7621 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     7681 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     7741 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     7801 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     7861 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     7921 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     7981 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     8041 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     8101 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGATT ACGCCCCGCC CTGCCACTCA 
     8161 TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA 
     8221 TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC 
     8281 ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG 
     8341 AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA 
     8401 TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG 
     8461 AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG 
     8521 GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG 
     8581 AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG 
     8641 TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA 
     8701 TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT 
     8761 ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATAC TCTTCCTTTT TCAATATTAT 
     8821 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8881 AATAAACAAA TAGGGGTCCC GCGCACATTT CCCCGAAAAG TGCCACCTGA C
//