LOCUS       RC224759                8334 bp    DNA     circular  SYN 
DEFINITION  SORBS1 (Myc-DDK-tagged)-Human sorbin and SH3 domain containing 1 (SORBS1), transcript variant 4
ACCESSION   NM_001034955
VERSION     NM_001034955.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4491..4582 
                     /label="Myc-DDK"  
     misc_feature    4612..5197 
                     /label="PolyA signal"  
     misc_feature    4669..4688 
                     /label="Restriction Enzyme"  
     misc_feature    5473..6145 
                     /label="ColE1"  
     misc_feature    6575..7369 
                     /label="Kan/Neo"  
     misc_feature    7404..7706 
                     /label="SV40 ori"  
     misc_feature    7792..8269 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4473..4479 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4473 
                     /label="SORBS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTCTGAA TGTGATGGTG GTTCCAAAGC TGTGATGAAT GGCTTGGCAC 
     1081 CTGGCAGCAA TGGGCAAGAC AAAGCAACTG CCGACCCTTT ACGCGCACGC TCTATTTCTG 
     1141 CTGTTAAAAT CATTCCTGTG AAGACAGTGA AAAACGCCTC AGGCCTAGTT CTCCCTACAG 
     1201 ACATGGATCC TACAAAAATC TGCACTGGGA AGGGAGCGGT GACTCTCCGG GCCTCGTCTT 
     1261 CCTACAGGGA AACCCCAAGC AGTAGCCCTG CGAGCCCTCA GGAAACCCGG CAACACGAAA 
     1321 GCAAACCAGG TCTGGAGCCA GAGCCTTCTT CAGCAGATGA GTGGAGGCTT TCTTCCAGTG 
     1381 CTGATGCCAA TGGAAATGCC CAGCCCTCTT CACTCGCTGC CAAGGGCTAC AGAAGTGTGC 
     1441 ATCCCAACCT TCCTTCTGAC AAGTCCCAGG ATGCCACTTC CTCCAGTGCA GCCCAGCCGG 
     1501 AGGTAATAGT TGTCCCTCTC TACCTGGTTA ATACTGACAG AGGGCAAGAA GGCACTGCCA 
     1561 GACCTCCAAC ACCTCTGGGG CCTCTTGGCT GCGTCCCCAC AATCCCAGCG ACTGCCTCTG 
     1621 CCGCCTCACC TCTGACCTTC CCGACTCTAG ATGATTTCAT TCCCCCTCAT CTGCAGAGGT 
     1681 GGCCCCACCA CAGCCAGCCA GCCCGCGCCT CTGGCTCCTT TGCCCCCATT AGCCAGACGC 
     1741 CACCATCCTT CTCACCACCA CCTCCGCTGG TCCCTCCTGC CCCGGAGGAC CTCCGCAGAG 
     1801 TCTCGGAGCC TGACCTCACG GGAGCTGTTT CGAGTACCGA TTCCAGTCCT CTACTAAATG 
     1861 AAGTTTCTTC TTCCCTTATT GGAACTGATT CCCAAGCCTT TCCATCAGTT AGCAAGCCTT 
     1921 CATCCGCCTA TCCCTCCACA ACGATTGTCA ATCCTACTAT TGTGCTCTTG CAACACAATC 
     1981 GAGAACAGCA AAAACGACTC AGTAGCCTTT CAGATCCTGT CTCAGAAAGA AGAGTGGGAG 
     2041 AGCAGGACTC AGCACCAACC CAGGAAAAAC CCACCTCACC TGGCAAGGCT ATTGAAAAAA 
     2101 GAGCAAAGGA TGACAGTAGG CGGGTGGTGA AGAGCACTCA GGACTTAAGC GATGTTTCCA 
     2161 TGGATGAAGT GGGCATCCCA CTCCGGAACA CTGAGAGATC AAAAGACTGG TACAAGACTA 
     2221 TGTTTAAACA GATCCACAAA CTGAACAGAG ACACTCCTGA AGAAAACCCT TATTTCCCTA 
     2281 CGTACAAATT CCCTGAACTT CCTGAAATCC AGCAAACTTC CGAAGAGACA AAGTCTTGCT 
     2341 CTGTCATGTC ACCCAGGCTG GAGTGCAGTG GTACAGTCAT AGCTCACTGC AGCCTCAAAC 
     2401 TCCTAGACTC AAGCAATCCT CCCACCTCAG CCTCCCAAGT AGCTGGGACT GCAGATGATG 
     2461 ATTCAGATCT GTACTCTCCC AGATACTCAT TTTCTGAAGA CACAAAATCT CCCCTTTCTG 
     2521 TGCCTCGCTC AAAAAGTGAG ATGAGCTACA TTGATGGTGA GAAGGTAGTC AAGAGGTCGG 
     2581 CCACACTACC CCTCCCAGCC CGCTCTTCCT CACTGAAGTC AAGCTCAGAA AGAAATGACT 
     2641 GGGAACCCCC AGATAAGAAA GTAGACACAA GAAAATATCG TGCAGAGCCC AAGAGCATTT 
     2701 ACGAATATCA GCCTGGCAAG TCTTCCGTTC TGACCAACGA AAAGATGAGT CGGGATATAA 
     2761 GCCCAGAAGA GATAGATTTA AAGAATGAAC CTTGGTATAA ATTCTTTTCG GAATTGGAGT 
     2821 TTGGGAAACC GCCTCCCAAA AAGATATGGG ATTATACTCC TGGAGACTGC TCTATCCTTC 
     2881 CTAGAGAGGA TAGAAAGACT AATCTAGACA AAGATCTCAG CCTCTGCCAG ACAGAGTTAG 
     2941 AGGCAGATTT AGAAAAAATG GAGACGCTTA ATAAAGCACC CAGTGCAAAC GTGCCACAGA 
     3001 GCTCAGCCAT CAGCCCTACT CCGGAAATTT CTTCAGAGAC TCCTGGATAT ATATATTCTT 
     3061 CCAACTTCCA TGCAGTGAAG AGGGAATCAG ACGGGGCTCC TGGGGATCTC ACTAGCTTGG 
     3121 AGAATGAGAG ACAAATTTAT AAAAGTGTCT TGGAAGGTGG TGACATCCCT CTTCAGGGCC 
     3181 TGAGTGGGCT CAAGCGACCA TCCAGCTCTG CTTCCACTAA AGATTCAGAA TCGCCAAGAC 
     3241 ATTTTATACC AGCTGATTAC TTGGAATCCA CGGAAGAATT TATTCGAAGA CGTCATGATG 
     3301 ATAAAGAGAA ACTTTTAGCG GACCAGAGAC GACTTAAACG CGAGCAAGAA GAGGCTGATA 
     3361 TTGCAGCTCG ACGCCACACA GGCGTCATTC CGACGCACCA TCAGTTTATC ACTAATGAGC 
     3421 GCTTTGGGGA CCTCCTCAAT ATAGACGATA CTGCAAAAAG GAAATCTGGG TCAGAGATGA 
     3481 GACCTGCCAG AGCCAAATTT GACTTTAAAG CTCAGACACT AAAGGAGCTT CCTCTGCAGA 
     3541 AGGGAGATAT TGTTTACATT TATAAGCAAA TTGATCAGAA CTGGTATGAA GGAGAACACC 
     3601 ACGGCCGGGT GGGAATCTTC CCACGCACCT ACATCGAGCT TCTTCCTCCT GCTGAGAAGG 
     3661 CACAGCCCAA AAAGTTGACA CCAGTGCAGG TTTTGGAATA TGGAGAAGCT ATTGCTAAGT 
     3721 TTAACTTTAA TGGTGATACA CAAGTAGAAA TGTCCTTCAG AAAGGGTGAG AGGATCACAC 
     3781 TGCTCCGGCA GGTAGATGAG AACTGGTACG AAGGGAGGAT CCCGGGGACA TCCCGACAAG 
     3841 GCATCTTCCC CATCACCTAC GTGGATGTGA TCAAGCGACC ACTGGTGAAA AACCCTGTGG 
     3901 ATTACATGGA CCTGCCTTTC TCCTCCTCCC CAAGTCGCAG TGCCACTGCA AGCCCACAGC 
     3961 CATGGCGCGA AGAGAGTGGA CAATATGAGA GGAAAGCAGA GAGGGGGGCA GGCGAAAGAG 
     4021 GCCCTGGTGG ACCCAAGATC TCTAAGAAGA GCTGCTTGAA GCCTTCAGAC GTGGTCAGGT 
     4081 GCCTGAGTAC TGAACAGAGA CTCTCAGATC TCAACACCCC TGAGGAGAGC CGGCCCGGCA 
     4141 AGCCCCTGGG TAGCGCTTTT CCAGGAAGTG AGGCTGAGCA GACAGAGCGG CATAGAGGTG 
     4201 GCGAGCAGGC GGGGAGGAAA GCTGCTCGGA GAGGTGGGAG CCAGCAACCT CAAGCCCAGC 
     4261 AGCGAAGAGT CACCCCCGAC AGGAGTCAAA CCTCACAAGA TTTATTTAGC TATCAAGCAT 
     4321 TATATAGCTA TATACCACAG AATGATGATG AGTTGGAACT CCGCGATGGA GATATCGTTG 
     4381 ATGTCATGGA AAAATGTGAC GATGGATGGT TTGTTGGTAC TTCAAGAAGG ACAAAGCAGT 
     4441 TTGGTACTTT TCCAGGCAAC TATGTAAAAC CTTTGTATCT AACGCGTACG CGGCCGCTCG 
     4501 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4561 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4621 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4681 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4741 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4801 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4861 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4921 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4981 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5041 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5101 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5161 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5221 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5281 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5341 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5401 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5461 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5521 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5581 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5641 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5701 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5761 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5821 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5881 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5941 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6001 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6061 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6121 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6181 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6241 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6301 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6361 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6421 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6481 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6541 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6601 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6661 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6721 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6781 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6841 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6901 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6961 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7021 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7081 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7141 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7201 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7261 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7321 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7381 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7441 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7501 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7561 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7621 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7681 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7741 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7801 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7861 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7921 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7981 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8041 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8101 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8161 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8221 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8281 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//