LOCUS       RC224758                8364 bp    DNA     circular  SYN 
DEFINITION  ITGAD (Myc-DDK-tagged)-Human integrin, alpha D (ITGAD)
ACCESSION   NM_005353
VERSION     NM_005353.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4521..4612 
                     /label="Myc-DDK"  
     misc_feature    4642..5227 
                     /label="PolyA signal"  
     misc_feature    4699..4718 
                     /label="Restriction Enzyme"  
     misc_feature    5503..6175 
                     /label="ColE1"  
     misc_feature    6605..7399 
                     /label="Kan/Neo"  
     misc_feature    7434..7736 
                     /label="SV40 ori"  
     misc_feature    7822..8299 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4503..4509 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4503 
                     /label="ITGAD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCTTCGGC ACTGTGCTTC TTCTGAGTGT CCTGGCTTCT TATCATGGAT 
     1081 TCAACCTGGA TGTGGAGGAG CCTACGATCT TCCAGGAGGA TGCAGGCGGC TTTGGGCAGA 
     1141 GCGTGGTGCA GTTCGGTGGA TCTCGACTCG TGGTGGGAGC ACCCCTGGAG GTGGTGGCGG 
     1201 CCAACCAGAC GGGACGGCTG TATGACTGCG CAGCTGCCAC CGGCATGTGC CAGCCCATCC 
     1261 CGCTGCACAT CCGCCCTGAG GCCGTGAACA TGTCCTTGGG CCTGACCCTG GCAGCCTCCA 
     1321 CCAACGGCTC CCGGCTCCTG GCCTGTGGCC CGACCCTGCA CAGAGTCTGT GGGGAGAACT 
     1381 CATACTCAAA GGGTTCCTGC CTCCTGCTGG GCTCGCGCTG GGAGATCATC CAGACAGTCC 
     1441 CCGACGCCAC GCCAGAGTGT CCACATCAAG AGATGGACAT CGTCTTCCTG ATTGACGGCT 
     1501 CTGGAAGCAT TGACCAAAAT GACTTTAACC AGATGAAGGG CTTTGTCCAA GCTGTCATGG 
     1561 GCCAGTTTGA GGGCACTGAC ACCCTGTTTG CACTGATGCA GTACTCAAAC CTCCTGAAGA 
     1621 TCCACTTCAC CTTCACCCAA TTCCGGACCA GCCCGAGCCA GCAGAGCCTG GTGGATCCCA 
     1681 TCGTCCAACT GAAAGGCCTG ACGTTCACGG CCACGGGCAT CCTGACAGTG GTGACACAGC 
     1741 TATTTCATCA TAAGAATGGG GCCCGAAAAA GTGCCAAGAA GATCCTCATT GTCATCACAG 
     1801 ATGGGCAGAA GTACAAAGAC CCCCTGGAAT ACAGTGATGT CATCCCCCAG GCAGAGAAGG 
     1861 CTGGCATCAT CCGCTACGCT ATCGGGGTGG GACACGCTTT CCAGGGACCC ACTGCCAGGC 
     1921 AGGAGCTGAA TACCATCAGC TCAGCGCCTC CGCAGGACCA CGTGTTCAAG GTGGACAACT 
     1981 TTGCAGCCCT TGGCAGCATC CAGAAGCAGC TGCAGGAGAA GATCTATGCA GTTGAGGGAA 
     2041 CCCAGTCCAG GGCAAGCAGC TCCTTCCAGC ACGAGATGTC CCAAGAAGGC TTCAGCACAG 
     2101 CCCTCACAAT GGATGGCCTC TTCCTGGGGG CTGTGGGGAG CTTTAGCTGG TCTGGAGGTG 
     2161 CCTTCCTGTA TCCCCCAAAT ATGAGCCCCA CCTTCATCAA CATGTCTCAG GAGAATGTGG 
     2221 ACATGAGGGA CTCTTACCTG GGTTACTCCA CCGAGCTAGC CCTGTGGAAG GGGGTACAGA 
     2281 ACCTGGTCCT GGGGGCCCCC CGCTACCAGC ATACCGGGAA GGCTGTCATC TTCACCCAGG 
     2341 TGTCCAGGCA ATGGAGGAAG AAGGCCGAAG TCACAGGGAC GCAGATCGGC TCCTACTTCG 
     2401 GGGCCTCCCT CTGCTCCGTG GATGTGGACA GCGATGGCAG CACCGACCTG ATCCTCATTG 
     2461 GGGCCCCCCA TTACTATGAG CAGACCCGAG GGGGCCAGGT GTCCGTGTGT CCCTTGCCTA 
     2521 GGGGGAGGGT GCAGTGGCAG TGTGACGCTG TTCTCCGTGG TGAGCAGGGC CACCCCTGGG 
     2581 GCCGCTTTGG GGCAGCCCTG ACAGTGTTGG GGGATGTGAA TGAGGACAAG CTGATAGACG 
     2641 TGGCCATTGG GGCCCCGGGA GAGCAGGAGA ACCGGGGTGC TGTCTACCTG TTTCACGGAG 
     2701 CCTCAGAATC CGGCATCAGC CCCTCCCACA GCCAGCGGAT TGCCAGCTCC CAGCTCTCCC 
     2761 CCAGGCTGCA GTATTTTGGG CAGGCGCTGA GTGGGGGTCA GGACCTCACC CAGGATGGAC 
     2821 TGATGGACCT GGCCGTGGGG GCCCGGGGCC AGGTGCTCCT GCTCAGGAGT CTGCCGGTGC 
     2881 TGAAAGTGGG GGTGGCCATG AGATTCAGCC CTGTGGAGGT GGCCAAGGCT GTGTACCGGT 
     2941 GCTGGGAAGA GAAGCCCAGT GCCCTGGAAG CTGGGGACGC CACCGTCTGT CTCACCATCC 
     3001 AGAAAAGCTC ACTGGACCAG CTAGGTGACA TCCAAAGCTC TGTCAGGTTT GATCTGGCAC 
     3061 TGGACCCAGG TCGTCTGACT TCTCGTGCCA TTTTCAATGA AACCAAGAAC CCCACTTTGA 
     3121 CTCGAAGAAA AACCCTGGGA CTGGGGATTC ACTGTGAAAC CCTGAAGCTG CTTTTGCCAG 
     3181 ATTGTGTGGA GGATGTGGTG AGCCCCATCA TTCTGCACCT CAACTTCTCA CTGGTGAGAG 
     3241 AGCCCATCCC CTCCCCCCAG AACCTGCGTC CTGTGCTGGC CGTGGGCTCA CAAGACCTCT 
     3301 TCACTGCTTC TCTCCCCTTC GAGAAGAACT GTGGGCAAGA TGGCCTCTGT GAAGGGGACC 
     3361 TGGGTGTCAC CCTCAGCTTC TCAGGCCTGC AGACCCTGAC CGTGGGGAGC TCCCTGGAGC 
     3421 TCAACGTGAT TGTGACTGTG TGGAACGCAG GTGAGGATTC CTACGGAACC GTGGTCAGCC 
     3481 TCTACTATCC AGCAGGGCTG TCGCACCGAC GGGTGTCAGG AGCCCAGAAG CAGCCCCATC 
     3541 AGAGTGCCCT GCGCCTGGCA TGTGAGACAG TGCCCACTGA GGATGAGGGC CTAAGAAGCA 
     3601 GCCGCTGCAG TGTCAACCAC CCCATCTTCC ATGAGGGCTC TAACGGCACC TTCATAGTCA 
     3661 CATTCGATGT CTCCTACAAG GCCACCCTGG GAGACAGGAT GCTTATGAGG GCCAGTGCAA 
     3721 GCAGTGAGAA CAATAAGGCT TCAAGCAGCA AGGCCACCTT CCAGCTGGAG CTCCCGGTGA 
     3781 AGTATGCAGT CTACACCATG ATCAGCAGGC AGGAAGAATC CACCAAGTAC TTCAACTTTG 
     3841 CAACCTCCGA TGAGAAGAAA ATGAAAGAGG CTGAGCATCG ATACCGTGTG AATAACCTCA 
     3901 GCCAGCGAGA TCTGGCCATC AGCATTAACT TCTGGGTTCC TGTCCTGCTG AACGGGGTGG 
     3961 CTGTGTGGGA TGTGGTCATG GAGGCCCCAT CTCAGAGTCT CCCCTGTGTT TCAGAGAGAA 
     4021 AACCTCCCCA GCATTCTGAC TTCCTGACCC AGATTTCAAG AAGTCCCATG CTGGACTGCT 
     4081 CCATTGCTGA CTGCCTGCAG TTCCGCTGTG ACGTCCCCTC CTTCAGCGTC CAGGAGGAGC 
     4141 TGGATTTCAC CCTGAAGGGC AATCTCAGTT TCGGCTGGGT CCGCGAGACA TTGCAGAAGA 
     4201 AGGTGTTGGT CGTGAGTGTG GCTGAAATTA CGTTCGACAC ATCCGTGTAC TCCCAGCTTC 
     4261 CAGGACAGGA GGCATTTATG AGAGCTCAGA TGGAGATGGT GCTAGAAGAA GACGAGGTCT 
     4321 ACAATGCCAT TCCCATCATC ATGGGCAGCT CTGTGGGGGC TCTGCTACTG CTGGCGCTCA 
     4381 TCACAGCCAC ACTGTACAAG CTTGGCTTCT TCAAACGCCA CTACAAGGAA ATGCTGGAGG 
     4441 ACAAGCCTGA AGACACTGCC ACATTCAGTG GGGACGATTT CAGCTGTGTG GCCCCAAATG 
     4501 TGCCTTTGTC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4561 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5341 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5401 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5461 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5521 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5581 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5641 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5701 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5761 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5821 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5881 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5941 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6001 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6061 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6121 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6181 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6241 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6301 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6361 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6421 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6481 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6541 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6601 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6661 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6721 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6781 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6841 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6901 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6961 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7021 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7081 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7141 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7201 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7261 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7321 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7381 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7441 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7501 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7561 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7801 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7861 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7921 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7981 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8041 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8101 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8161 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8221 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8281 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8341 TAACAAAAAT TTAACGCGAA TTTT
//