LOCUS       RC224753                7998 bp    DNA     circular  SYN 
DEFINITION  B4GALNT4 (Myc-DDK-tagged)-Human beta-1,4-N-acetyl-galactosaminyl transferase 4 (B4GALNT4)
ACCESSION   NM_178537
VERSION     NM_178537.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4155..4246 
                     /label="Myc-DDK"  
     misc_feature    4276..4861 
                     /label="PolyA signal"  
     misc_feature    4333..4352 
                     /label="Restriction Enzyme"  
     misc_feature    5137..5809 
                     /label="ColE1"  
     misc_feature    6239..7033 
                     /label="Kan/Neo"  
     misc_feature    7068..7370 
                     /label="SV40 ori"  
     misc_feature    7456..7933 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4137..4143 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4137 
                     /label="B4GALNT4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGCGGCTC CCGGTGAAGA AGATCCGTAA GCAGATGAAG CTGCTGCTGC 
     1081 TGCTGCTGCT GCTGAGCTGC GCCGCGTGGC TCACCTACGT GCACCTGGGC CTGGTGCGCC 
     1141 AGGGACGCGC GCTGCGCCAG CGCCTGGGCT ACGGGCGAGA TGGTGAGAAG CTGACCAGTG 
     1201 AGACCGACGG CCGGGGGGTC CACGCTGCGC CATCCACACA GAGGGCTGAG GACTCCAGTG 
     1261 AGAGCCGTGA AGAGGAGCAA GCGCCCGAAG GTCGGGACCT AGACATGCTG TTTCCTGGGG 
     1321 GGGCTGGGAG GCTGCCACTG AACTTCACCC ATCAGACACC CCCATGGCGG GAGGAGTACA 
     1381 AGGGGCAGGT GAACCTGCAC GTGTTTGAGG ACTGGTGTGG GGGCGCCGTG GGCCACCTGA 
     1441 GGAGGAACCT GCACTTCCCG CTGTTCCCTC ATACGCGCAC CACCGTGAAG AAGTTGGCCG 
     1501 TGTCCCCCAA GTGGAAGAAC TATGGACTCC GTATTTTTGG TTTCATCCAC CCGGCGAGGG 
     1561 ACGGAGACGT CCAGTTTTCT GTGGCCTCAG ACGACAACTC GGAGTTCTGG CTGAGTCTGG 
     1621 ACGAGAGCCC TGCTGCTGCC CAGCTTGTGG CCTTTGTGGG CAAGACTGGC TCCGAGTGGA 
     1681 CAGCGCCTGG AGAATTCACC AAGTTCAGCT CCCAGGTGTC CAAGCCCAGG CGGCTCATGG 
     1741 CCTCCCGGAG GTACTACTTT GAGTTGCTGC ACAAGCAGGA CGACCGCGGC TCGGACCACG 
     1801 TGGAAGTGGG CTGGCGAGCT TTCCTGCCCG GCCTGAAGTT CGAGGTCATC AGCTCTGCTC 
     1861 ACATCTCCCT GTACACAGAT GAGTCAGCCT TGAAGATGGA CCACGTGGCG CACGTCCCCC 
     1921 AGTCTCCAGC CAGCCACGTG GGGGGGCGTC CGCCGCAGGA GGAGACCAGC GCAGACATGC 
     1981 TGCGGCCAGA TCCCAGGGAT ACCTTTTTCC TCACTCCACG CATGGAATCT TCGAGCCTGG 
     2041 AGAACGTGCT GGAGCCCTGC GCCTACGCCC CCACCTACGT GGTCAAGGAC TTCCCGATCG 
     2101 CCAGATACCA GGGCCTGCAA TTTGTGTACC TGTCCTTCGT TTATCCCAAC GACTACACTC 
     2161 GCCTCACCCA CATGGAGACG GACAACAAGT GCTTCTACCG CGAGTCTCCG CTGTATCTGG 
     2221 AGAGGTTTGG GTTCTATAAA TACATGAAGA TGGACAAGGA GGAGGGGGAT GAGGATGAAG 
     2281 AAGACGAGGT GCAGCGCCGA GCCTTCCTCT TCCTCAACCC GGACGACTTC CTGGACGACG 
     2341 AGGACGAGGG GGAGCTGCTC GACAGCCTGG AGCCCACCGA GGCGGCCCCG CCCAGGAGCG 
     2401 GCCCCCAGTC CCCCGCCCCA GCAGCCCCCG CCCAGCCCGG AGCCACCCTC GCCCCGCCGA 
     2461 CCCCTCCCCG CCCCCGGGAC GGGGGGACCC CCAGGCACTC CCGGGCCCTG AGCTGGGCCG 
     2521 CCAGGGCCGC CCGCCCTTTG CCGCTCTTCT TGGGCCGAGC TCCGCCCCCG CGCCCTGCAG 
     2581 TGGAGCAGCC GCCCCCAAAG GTGTACGTGA CCAGGGTGCG GCCGGGACAG CGGGCATCCC 
     2641 CCCGGGCCCC AGCGCCGCGT GCGCCCTGGC CGCCCTTCCC TGGCGTCTTC CTGCACCCCA 
     2701 GGCCTCTGCC CAGAGTGCAG CTGCGGGCGC CCCCACGCCC ACCCCGGCCC CACGGCCGCA 
     2761 GGACCGGCGG CCCCCAGGCC ACACAGCCGA GGCCCCCAGC CCGGGCGCAG GCCACCCAAG 
     2821 GGGGCCGGGA GGGCCAGGCG CGCACGCTGG GACCTGCGGC GCCCACAGTG GACTCAAACT 
     2881 TGTCCTCCGA AGCGCGGCCC GTGACCTCCT TCCTGAGCTT GTCCCAGGTG TCCGGGCCGC 
     2941 AGCTGCCCGG GGAGGGCGAA GAGGAGGAGG AAGGGGAGGA CGATGGGGCC CCGGGCGACG 
     3001 AGGCCGCGTC GGAGGACAGC GAGGAGGCCG CGGGCCCGGC GCTCGGACGC TGGCGTGAGG 
     3061 ACGCCATCGA CTGGCAGCGC ACGTTCAGCG TGGGCGCCGT GGACTTCGAG CTGCTGCGCT 
     3121 CGGACTGGAA CGACCTGCGA TGCAACGTTT CGGGGAACCT GCAGCTGCCG GAGGCGGAGG 
     3181 CCGTGGACGT GACCGCTCAG TACATGGAGC GGCTGAACGC GCGCCACGGC GGGCGCTTCG 
     3241 CGCTTCTGCG CATCGTGAAC GTGGAGAAGC GCCGGGACTC GGCGCGAGGG AGTCGCTTCC 
     3301 TGCTGGAGCT GGAGCTGCAG GAGCGCGGGG GCGGCCGCCT GCGACTGTCC GAGTACGTCT 
     3361 TCCTGCGGCT GCCGGGAGCC CGCGTAGGGG ATGCAGACGG AGAAAGTCCC GAACCCGCTC 
     3421 CCGCCGCCTC CGTGCGCCCC GACGGCCGCC CCGAGCTCTG CCGGCCACTG CGCCTGGCCT 
     3481 GGCGCCAGGA CGTGATGGTT CACTTCATCG TGCCAGTGAA AAACCAGGCA CGGTGGGTGG 
     3541 CACAGTTCCT GGCGGACATG GCTGCGCTGC ACGCGCGCAC CGGGGACTCG CGTTTCAGCG 
     3601 TCGTCCTGGT GGATTTCGAG AGCGAGGATA TGGACGTGGA GCGGGCCCTG CGCGCCGCGC 
     3661 GCCTGCCCCG GTACCAGTAC CTGAGACGAA CCGGGAACTT CGAGCGCTCC GCCGGGCTGC 
     3721 AGGCGGGAGT GGACGCGGTA GAGGACGCCA GCAGCATCGT GTTCCTCTGC GACCTGCACA 
     3781 TCCACTTCCC ACCCAACATC CTGGACGGCA TCCGCAAGCA CTGCGTGGAG GGCAGGCTGG 
     3841 CCTTCGCGCC CGTGGTCATG CGCCTGAGCT GCGGGAGCTC GCCCCGGGAC CCCCACGGTT 
     3901 ACTGGGAGGT GAACGGCTTT GGCCTTTTTG GGATCTACAA GTCGGACTTT GACCGGGTTG 
     3961 GAGGAATGAA CACGGAGGAG TTCCGAGACC AGTGGGGGGG TGAAGACTGG GAGCTCCTGG 
     4021 ACAGGGTCCT GCAGGCAGGG CTGGAGGTGG AGCGGCTCCG ACTGCGGAAT TTCTATCACC 
     4081 ACTACCACTC CAAGAGGGGC ATGTGGAGCG TCCGCAGCAG GAAGGGCTCT CGCACGGGGG 
     4141 CGTCTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4201 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4261 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4321 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4381 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4441 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4501 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4561 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4621 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4681 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4741 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4801 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4861 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4921 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4981 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5041 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5101 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5161 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5221 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5281 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5341 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5401 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5461 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5521 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5581 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5641 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5701 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5761 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5821 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5881 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5941 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6001 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6061 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6121 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6181 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6241 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6301 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6361 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6421 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6481 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6541 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6601 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6661 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6721 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6781 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6841 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6901 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6961 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7021 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7081 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7141 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7201 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7261 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7321 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7381 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7441 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7501 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7561 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7621 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7681 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7741 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7801 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7861 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7921 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7981 AAATTTAACG CGAATTTT
//