LOCUS       RC224610                8889 bp    DNA     circular  SYN 
DEFINITION  GRIN2D (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 2D (GRIN2D)
ACCESSION   NM_000836
VERSION     NM_000836.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5046..5137 
                     /label="Myc-DDK"  
     misc_feature    5167..5752 
                     /label="PolyA signal"  
     misc_feature    5224..5243 
                     /label="Restriction Enzyme"  
     misc_feature    6028..6700 
                     /label="ColE1"  
     misc_feature    7130..7924 
                     /label="Kan/Neo"  
     misc_feature    7959..8261 
                     /label="SV40 ori"  
     misc_feature    8347..8824 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5028..5034 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5028 
                     /label="GRIN2D"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCGGCGCC GGTGGCCCCC GCGGCCCTCG GGGCCCCGCT AAGATGCTGC 
     1081 TGCTGCTGGC GCTGGCCTGC GCCAGCCCGT TCCCGGAGGA GGCGCCGGGG CCGGGCGGGG 
     1141 CCGGTGGGCC CGGCGGCGGC CTCGGCGGGG CGCGGCCGCT CAACGTGGCG CTCGTGTTCT 
     1201 CGGGGCCCGC GTACGCGGCC GAGGCGGCAC GCCTGGGCCC GGCCGTGGCG GCGGCGGTGC 
     1261 GCAGCCCGGG CCTAGACGTG CGGCCCGTGG CGCTGGTGCT CAACGGCTCG GACCCGCGCA 
     1321 GCCTCGTGCT GCAGCTCTGC GACCTGCTGT CGGGGTTGCG CGTGCACGGC GTGGTCTTCG 
     1381 AAGACGACTC GCGCGCGCCC GCCGTCGCGC CCATCCTCGA CTTCCTGTCG GCGCAGACCT 
     1441 CGCTGCCCAT CGTGGCCGTG CACGGCGGCG CCGCGCTCGT GCTCACGCCC AAGGAGAAGG 
     1501 GCTCCACCTT CCTGCAGCTG GGCTCTTCCA CCGAGCAACA GCTTCAGGTC ATCTTTGAGG 
     1561 TGCTGGAGGA GTATGACTGG ACGTCCTTTG TAGCCGTGAC CACTCGTGCC CCTGGCCACC 
     1621 GGGCCTTCCT GTCCTACATT GAGGTGCTGA CTGACGGTAG TCTGGTGGGC TGGGAGCACC 
     1681 GCGGAGCGCT GACGCTGGAC CCTGGGGCGG GCGAGGCCGT GCTCAGTGCC CAGCTCCGCA 
     1741 GTGTCAGCGC GCAGATCCGC CTGCTCTTCT GCGCCCGAGA GGAGGCCGAG CCCGTGTTCC 
     1801 GCGCAGCTGA GGAGGCTGGC CTCACTGGAT CTGGCTACGT CTGGTTCATG GTGGGGCCCC 
     1861 AGCTGGCTGG AGGCGGGGGC TCTGGGGCCC CTGGTGAGCC CCCTCTTCTG CCAGGAGGCG 
     1921 CCCCCCTGCC TGCCGGGCTG TTTGCAGTGC GCTCGGCTGG CTGGCGGGAT GACCTGGCTC 
     1981 GGCGAGTGGC AGCTGGCGTG GCCGTAGTGG CCAGAGGTGC CCAGGCCCTG CTGCGTGATT 
     2041 ATGGTTTCCT TCCTGAGCTC GGCCACGACT GTCGCGCCCA GAACCGCACC CACCGCGGCG 
     2101 AGAGTCTGCA TAGGTACTTC ATGAACATCA CGTGGGATAA CCGGGATTAC TCCTTCAATG 
     2161 AGGACGGCTT CCTAGTGAAC CCCTCCCTGG TGGTCATCTC CCTCACCAGA GACAGGACGT 
     2221 GGGAGGTGGT GGGCAGCTGG GAGCAGCAGA CGCTCCGCCT CAAGTACCCG CTGTGGTCCC 
     2281 GCTATGGTCG CTTCCTGCAG CCAGTGGACG ACACGCAGCA CCTCACGGTG GCCACGCTGG 
     2341 AGGAAAGGCC GTTTGTCATC GTGGAGCCTG CAGACCCTAT CAGCGGCACC TGCATCCGAG 
     2401 ACTCCGTCCC CTGCCGGAGC CAGCTCAACC GAACCCACAG CCCTCCACCG GATGCCCCCC 
     2461 GCCCGGAAAA GCGCTGCTGC AAGGGTTTCT GCATCGACAT TCTGAAGCGG CTGGCGCATA 
     2521 CCATCGGCTT CAGCTACGAC CTCTACCTGG TCACCAATGG CAAGCACGGA AAGAAGATCG 
     2581 ATGGCGTCTG GAACGGCATG ATCGGGGAGG TGTTCTACCA GCGCGCAGAC ATGGCCATCG 
     2641 GCTCCCTCAC CATCAACGAG GAGCGCTCCG AGATCGTGGA CTTCTCCGTC CCCTTCGTGG 
     2701 AGACCGGCAT CAGCGTCATG GTGGCGCGCA GCAATGGCAC GGTGTCCCCC TCGGCCTTCC 
     2761 TCGAGCCCTA CAGCCCCGCC GTGTGGGTGA TGATGTTCGT CATGTGCCTC ACTGTGGTCG 
     2821 CCGTCACTGT TTTCATCTTC GAGTACCTCA GTCCTGTTGG TTACAACCGC AGCCTGGCCA 
     2881 CGGGCAAGCG CCCTGGCGGT TCAACCTTCA CCATTGGGAA ATCCATCTGG CTGCTCTGGG 
     2941 CCCTGGTGTT CAATAATTCG GTGCCCGTGG AGAACCCCCG GGGAACCACC AGCAAAATCA 
     3001 TGGTGCTGGT GTGGGCCTTC TTCGCCGTCA TCTTCCTCGC CAGCTACACA GCCAACCTGG 
     3061 CCGCCTTCAT GATCCAGGAG GAGTACGTGG ATACTGTGTC TGGGCTCAGT GACCGCAAGT 
     3121 TCCAGAGGCC CCAGGAGCAG TACCCGCCCC TGAAGTTTGG GACCGTGCCC AACGGCTCCA 
     3181 CGGAGAAGAA CATCCGCAGC AACTATCCCG ACATGCACAG CTACATGGTG CGCTACAACC 
     3241 AGCCCCGCGT AGAGGAAGCG CTCACTCAGC TCAAGGCAGG GAAGCTGGAC GCCTTCATCT 
     3301 ACGATGCTGC AGTGCTCAAT TACATGGCCC GCAAGGACGA GGGCTGCAAG CTTGTCACCA 
     3361 TCGGCTCCGG CAAGGTCTTC GCCACGACAG GCTATGGCAT CGCCCTGCAC AAGGGCTCCC 
     3421 GCTGGAAGCG GCCCATCGAC CTGGCGTTGC TGCAGTTCCT GGGGGATGAT GAGATCGAGA 
     3481 TGCTGGAGCG GCTGTGGCTC TCTGGGATCT GCCACAATGA CAAAATCGAG GTGATGAGCA 
     3541 GCAAGCTGGA CATCGACAAC ATGGCGGGCG TCTTCTACAT GCTCCTGGTG GCCATGGGCC 
     3601 TGTCCCTGCT GGTCTTCGCC TGGGAGCACC TGGTGTACTG GCGCCTGCGG CACTGCCTGG 
     3661 GGCCCACCCA CCGCATGGAC TTCCTGCTGG CCTTCTCCAG GGGCATGTAC AGCTGCTGCA 
     3721 GCGCTGAGGC CGCCCCACCG CCCGCCAAGC CCCCGCCGCC GCCACAGCCC CTGCCCAGCC 
     3781 CCGCGTACCC CGCGCCGCGG CCGGCTCCCG GGCCCGCACC TTTCGTGCCC CGCGAGCGCG 
     3841 CCTCAGTGGA CCGCTGGCGC CGGACCAAGG GCGCGGGGCC GCCGGGGGGC GCGGGCCTGG 
     3901 CCGACGGCTT CCACCGCTAC TACGGCCCCA TCGAGCCGCA GGGCCTAGGC CTCGGCCTGG 
     3961 GCGAAGCGCG CGCGGCACCG CGGGGCGCAG CCGGGCGCCC GCTGTCCCCG CCGGCCGCTC 
     4021 AGCCCCCGCA GAAGCCGCCG CCCTCCTATT TCGCCATCGT ACGCGACAAG GAGCCAGCCG 
     4081 AGCCCCCCGC CGGCGCCTTC CCCGGCTTCC CGTCGCCGCC CGCGCCCCCC GCCGCCGCGG 
     4141 CCACCGCCGT CGGGCCGCCA CTCTGCCGCT TGGCCTTCGA GGACGAGAGC CCGCCGGCGC 
     4201 CCGCGCGGTG GCCGCGCTCG GACCCCGAGA GCCAACCCCT GCTGGGGCCA GGCGCGGGCG 
     4261 GCGCGGGGGG CACGGGGGGC GCAGGCGGAG GAGCCCCGGC CGCTCCGCCC CCGTGCCGCG 
     4321 CCGCGCCGCC CCCGTGCCCT TACCTCGATC TCGAGCCGTC GCCGTCGGAC TCGGAGGACT 
     4381 CGGAGAGCCT GGGCGGCGCG TCGCTGGGCG GCCTGGAGCC CTGGTGGTTC GCCGACTTCC 
     4441 CTTACCCGTA TGCCGAGCGC CTCGGGCCGC CGCCCGGCCG CTACTGGTCG GTCGACAAGC 
     4501 TCGGGGGCTG GCGCGCCGGG AGCTGGGACT ACCTGCCCCC GCGCAGCGGT CCGGCCGCCT 
     4561 GGCACTGTCG GCACTGCGCC AGCCTGGAGC TGCTGCCGCC GCCGCGCCAT CTCAGCTGCT 
     4621 CGCACGATGG CCTGGACGGC GGCTGGTGGG CGCCACCGCC TCCACCCTGG GCCGCCGGGC 
     4681 CCCTGCCCCG ACGCCGGGCC CGCTGCGGGT GCCCGCGGTC GCACCCGCAC CGCCCGCGGG 
     4741 CCTCGCACCG CACGCCCGCC GCCGCCGCGC CCCACCACCA CAGGCACCGG CGCGCCGCTG 
     4801 GGGGCTGGGA CCTCCCGCCG CCCGCGCCCA CCTCGCGCTC GCTCGAGGAC CTCAGCTCGT 
     4861 GCCCTCGCGC CGCCCCTGCG CGCAGGCTTA CCGGGCCCTC CCGCCACGCT CGCAGGTGTC 
     4921 CGCACGCCGC GCACTGGGGG CCGCCGCTGC CCACAGCTTC CCACCGGAGA CACCGGGGCG 
     4981 GGGACCTGGG CACCCGCAGG GGCTCGGCGC ACTTCTCTAG CCTCGAGTCC GAGGTAACGC 
     5041 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5101 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5161 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5221 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5281 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5341 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5401 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5461 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5521 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5581 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5641 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5701 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5761 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5821 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5881 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5941 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6001 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6061 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6121 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6181 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6241 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6301 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6361 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6421 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6481 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6541 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6601 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6661 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6721 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6781 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6841 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6901 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6961 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7021 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7081 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7141 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7201 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7261 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7321 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7381 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7441 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7501 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7561 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7621 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7681 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7741 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7801 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7861 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7921 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7981 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8041 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8101 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8221 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8281 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8341 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8401 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8461 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8521 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8581 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8641 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8701 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8761 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8821 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8881 GCGAATTTT
//