LOCUS       RC224481                7998 bp    DNA     circular  SYN 
DEFINITION  OGT (Myc-DDK-tagged)-Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 2
ACCESSION   NM_181673
VERSION     NM_181673.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4153..4244 
                     /label="Myc-DDK"  
     misc_feature    4274..4859 
                     /label="PolyA signal"  
     misc_feature    4331..4350 
                     /label="Restriction Enzyme"  
     misc_feature    5135..5807 
                     /label="ColE1"  
     misc_feature    6237..7031 
                     /label="Kan/Neo"  
     misc_feature    7066..7368 
                     /label="SV40 ori"  
     misc_feature    7454..7931 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4128..4135 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4128 
                     /label="OGT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTCTTCC GTGGGCAACG TGGCCGACAG CACAGGGTTA GCTGAGTTGG 
     1081 CACATCGAGA ATATCAGGCA GGAGATTTTG AGGCAGCTGA GAGACACTGC ATGCAGCTCT 
     1141 GGAGACAAGA GCCAGACAAT ACTGGTGTGC TTTTATTACT TTCATCTATA CACTTCCAGT 
     1201 GTCGAAGGCT GGACAGATCT GCTCACTTTA GCACTCTGGC AATTAAACAG AACCCCCTTC 
     1261 TGGCAGAAGC TTATTCGAAT TTGGGGAATG TGTACAAGGA AAGAGGGCAG TTGCAGGAGG 
     1321 CAATTGAGCA TTATCGACAT GCATTGCGTC TCAAACCTGA TTTCATCGAT GGTTATATTA 
     1381 ACCTGGCAGC CGCCTTGGTA GCAGCGGGTG ACATGGAAGG GGCAGTACAA GCTTACGTCT 
     1441 CTGCTCTTCA GTACAATCCT GATTTGTACT GTGTTCGCAG TGACCTGGGG AACCTGCTCA 
     1501 AAGCCCTGGG TCGCTTGGAA GAAGCCAAGG CATGTTATTT GAAAGCAATT GAGACGCAAC 
     1561 CGAACTTTGC AGTAGCTTGG AGTAATCTTG GCTGTGTTTT CAATGCACAA GGGGAAATTT 
     1621 GGCTTGCAAT TCATCACTTT GAAAAGGCTG TCACCCTTGA CCCAAACTTT CTGGATGCTT 
     1681 ATATCAATTT AGGAAATGTC TTGAAAGAGG CACGCATTTT TGACAGAGCT GTGGCAGCTT 
     1741 ATCTTCGTGC CCTAAGTTTG AGTCCAAATC ACGCAGTGGT GCACGGCAAC CTGGCTTGTG 
     1801 TATACTATGA GCAAGGCCTG ATAGATCTGG CAATAGACAC CTACAGGCGG GCTATCGAAC 
     1861 TACAACCACA TTTCCCTGAT GCTTACTGCA ACCTAGCCAA TGCTCTCAAA GAGAAGGGCA 
     1921 GTGTTGCTGA AGCAGAAGAT TGTTATAATA CAGCTCTCCG TCTGTGTCCC ACCCATGCAG 
     1981 ACTCTCTGAA TAACCTAGCC AATATCAAAC GAGAACAGGG AAACATTGAA GAGGCAGTTC 
     2041 GCTTGTATCG TAAAGCATTA GAAGTCTTCC CAGAGTTTGC TGCTGCCCAT TCAAATTTAG 
     2101 CAAGTGTACT GCAGCAGCAG GGAAAACTGC AGGAAGCTCT GATGCATTAT AAGGAGGCTA 
     2161 TTCGAATCAG TCCTACCTTT GCTGATGCCT ACTCTAATAT GGGAAACACT CTAAAGGAGA 
     2221 TGCAGGATGT TCAGGGAGCC TTGCAGTGTT ATACGCGTGC CATCCAAATT AATCCTGCAT 
     2281 TTGCAGATGC ACATAGCAAT CTGGCTTCCA TTCATAAGGA TTCAGGGAAT ATTCCAGAAG 
     2341 CCATAGCTTC TTACCGCACG GCTCTGAAAC TTAAGCCTGA TTTTCCTGAT GCTTATTGTA 
     2401 ACTTGGCTCA TTGCCTGCAG ATTGTCTGTG ATTGGACAGA CTATGATGAG CGAATGAAGA 
     2461 AGTTGGTCAG TATTGTGGCT GACCAGTTAG AGAAGAATAG GTTGCCTTCT GTGCATCCTC 
     2521 ATCATAGTAT GCTATATCCT CTTTCTCATG GCTTCAGGAA GGCTATTGCT GAGAGGCACG 
     2581 GCAACCTGTG CTTAGATAAG ATTAATGTTC TTCATAAACC ACCATATGAA CATCCAAAAG 
     2641 ACTTGAAGCT CAGTGATGGT CGGCTGCGTG TAGGATATGT GAGTTCCGAC TTTGGGAATC 
     2701 ATCCTACTTC TCACCTTATG CAGTCTATTC CAGGCATGCA CAATCCTGAT AAATTTGAGG 
     2761 TGTTCTGTTA TGCCCTGAGC CCAGACGATG GCACAAACTT CCGAGTGAAG GTGATGGCAG 
     2821 AAGCCAATCA TTTCATTGAT CTTTCTCAGA TTCCATGCAA TGGAAAAGCA GCTGATCGCA 
     2881 TCCATCAGGA TGGAATTCAT ATCCTTGTAA ATATGAATGG CTATACTAAG GGCGCTCGAA 
     2941 ATGAGCTTTT TGCTCTCAGG CCAGCTCCTA TTCAGGCAAT GTGGCTGGGA TACCCTGGGA 
     3001 CGAGTGGTGC GCTTTTCATG GATTATATTA TCACTGATCA GGAAACTTCG CCAGCTGAAG 
     3061 TTGCTGAGCA GTATTCCGAG AAATTGGCTT ATATGCCCCA CACTTTTTTT ATTGGTGATC 
     3121 ATGCTAATAT GTTCCCTCAC CTGAAGAAAA AAGCAGTCAT CGATTTTAAG TCCAATGGGC 
     3181 ACATTTATGA CAATCGGATA GTTCTGAATG GCATCGACCT CAAAGCATTT CTTGATAGTC 
     3241 TACCAGATGT GAAAATTGTC AAGATGAAGT GTCCTGATGG AGGAGACAAT GCAGATAGCA 
     3301 GTAACACAGC TCTTAATATG CCTGTTATTC CTATGAATAC TATTGCAGAA GCAGTTATTG 
     3361 AAATGATTAA CCGAGGACAG ATTCAAATAA CAATTAATGG ATTCAGTATT AGCAATGGAC 
     3421 TGGCAACTAC TCAGATCAAC AATAAGGCTG CAACTGGAGA GGAGGTTCCC CGTACCATTA 
     3481 TTGTAACCAC CCGTTCTCAG TACGGGTTAC CAGAAGATGC CATCGTATAC TGTAACTTTA 
     3541 ATCAGTTGTA TAAAATTGAC CCTTCTACTT TGCAGATGTG GGCAAACATT CTGAAGCGTG 
     3601 TTCCCAATAG TGTACTCTGG CTGTTGCGTT TTCCAGCAGT AGGAGAACCT AATATTCAAC 
     3661 AGTATGCACA AAACATGGGC CTGCCCCAGA ACCGTATCAT TTTTTCACCT GTTGCTCCTA 
     3721 AAGAGGAACA CGTCAGGAGA GGCCAGCTGG CTGATGTCTG CTTGGACACT CCACTCTGTA 
     3781 ATGGGCACAC CACAGGGATG GATGTCCTCT GGGCAGGGAC CCCCATGGTG ACTATGCCAG 
     3841 GAGAGACTCT TGCTTCTCGA GTTGCAGCAT CCCAGCTCAC TTGCTTAGGT TGTCTTGAGC 
     3901 TTATTGCTAA AAACAGACAA GAATATGAAG ACATAGCTGT GAAGCTGGGA ACTGATCTAG 
     3961 AATACCTGAA GAAAGTTCGT GGCAAAGTCT GGAAGCAAAG AATATCTAGC CCTCTGTTCA 
     4021 ACACCAAACA ATACACAATG GAACTAGAGC GGCTCTATCT ACAGATGTGG GAGCATTATG 
     4081 CAGCTGGCAA CAAACCTGAC CACATGATTA AGCCTGTTGA AGTCACTGAG TCAGCAAGCG 
     4141 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4201 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4261 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4321 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4381 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4441 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4501 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4561 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4621 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4681 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4741 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4801 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4861 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4921 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4981 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5041 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5101 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5161 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5221 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5281 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5341 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5401 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5461 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5521 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5581 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5641 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5701 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5761 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5821 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5881 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5941 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6001 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6061 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6121 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6181 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6241 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6301 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6361 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6421 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6481 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6541 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6601 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6661 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6721 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6781 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6841 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6901 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6961 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7021 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7081 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7141 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7201 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7261 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7321 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7381 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7441 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7501 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7561 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7621 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7681 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7741 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7801 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7861 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7921 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7981 AAATTTAACG CGAATTTT
//