LOCUS       RC224416                8952 bp    DNA     circular  SYN 
DEFINITION  PLIN4 (Myc-DDK-tagged)-Human perilipin 4 (PLIN4)
ACCESSION   NM_001080400
VERSION     NM_001080400.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5109..5200 
                     /label="Myc-DDK"  
     misc_feature    5230..5815 
                     /label="PolyA signal"  
     misc_feature    5287..5306 
                     /label="Restriction Enzyme"  
     misc_feature    6091..6763 
                     /label="ColE1"  
     misc_feature    7193..7987 
                     /label="Kan/Neo"  
     misc_feature    8022..8324 
                     /label="SV40 ori"  
     misc_feature    8410..8887 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5091..5097 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5091 
                     /label="PLIN4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGACCCTG GGCAGCTTCT TTGGGTCCCT GCCTGGCTTC AGCTCTGCCC 
     1081 GGAACCTGGT GGCCAACGCA CATAGCTCGG CGAGAGCCCG GCCGGCCGCT GACCCCACAG 
     1141 GAGCGCCTGC TGCCGAGGCT GCCCAACCAC AGGCTCAGGT GGCTGCCCAC CCAGAGCAGA 
     1201 CGGCCCCATG GACGGAGAAG GAGCTGCAAC CTTCGGAAAA GCAGATGGTG TCCGGGGCCA 
     1261 AAGACCTGGT GTGTTCCAAG ATGTCCAGGG CCAAGGATGC CGTGTCCTCC GGGGTGGCCA 
     1321 GCGTGGTGGA CGTGGCTAAG GGAGTGGTCC AGGGAGGCCT GGACACCACT CGGTCTGCAC 
     1381 TTACGGGCAC CAAGGAGGTG GTGTCCAGCG GGGTCACAGG GGCCATGGAC ATGGCTAAGG 
     1441 GGGCCGTCCA AGGGGGTCTG GACACCTCGA AGGCTGTCCT CACCGGCACC AAGGACACGG 
     1501 TGTCCACTGG GCTCACGGGG GCAGTGAATG TGGCCAAAGG GACCGTACAG GCCGGTGTGG 
     1561 ACACCACCAA GACTGTGCTG ACCGGCACCA AAGACACAGT GACTACTGGG GTCATGGGGG 
     1621 CAGTGAACTT GGCCAAAGGG ACTGTCCAGA CTGGCGTGGA AACCTCCAAG GCTGTGCTGA 
     1681 CCGGCACCAA AGATGCTGTG TCCACTGGGC TCACAGGGGC AGTGAATGTG GCCAGAGGAA 
     1741 GCATTCAGAC CGGTGTGGAC ACCAGTAAGA CTGTCCTAAC AGGTACCAAG GACACCGTCT 
     1801 GTAGTGGGGT GACTGGTGCC ATGAATGTGG CCAAAGGAAC CATCCAGACC GGCGTGGACA 
     1861 CCAGTAAGAC TGTCCTAACA GGTACCAAGG ACACCGTCTG TAGTGGGGTG ACTGGTGCCA 
     1921 TGAATGTGGC CAAAGGAACC ATCCAGACCG GCGTGGACAC CAGTAAGACT GTCCTAACAG 
     1981 GTACCAAGGA CACCGTCTGT AGTGGGGTGA CTGGTGCCAT GAATGTGGCC AAAGGAACCA 
     2041 TCCAGACCGG CGTGGACACC ACCAAGACTG TCCTAACTGG CACCAAGAAC ACTGTCTGCA 
     2101 GTGGGGTGAC CGGTGCCGTG AACTTGGCCA AAGAGGCCAT CCAGGGGGGC CTGGATACCA 
     2161 CCAAGTCTAT GGTCATGGGT ACGAAAGACA CGATGTCCAC TGGGCTCACA GGGGCAGCGA 
     2221 ATGTGGCCAA GGGGGCCATG CAAACTGGGC TGAACACAAC CCAAAATATC GCAACAGGTA 
     2281 CAAAGGACAC CGTCTGCAGT GGGGTGACTG GTGCCATGAA TTTGGCCAGA GGAACCATCC 
     2341 AGACAGGCGT GGACACCACC AAGATCGTTC TAACTGGTAC CAAGGACACT GTCTGCAGTG 
     2401 GGGTCACCGG TGCTGCGAAT GTGGCCAAAG GGGCCGTCCA GGGCGGCCTG GACACTACAA 
     2461 AGTCTGTCCT GACTGGCACT AAAGATGCTG TGTCCACTGG GCTCACAGGG GCTGTGAACG 
     2521 TGGCCAAAGG GACCGTCCAG ACCGGCGTAG ACACCACCAA GACTGTCCTA ACCGGCACCA 
     2581 AGGACACCGT CTGCAGTGGG GTGACCAGTG CTGTGAACGT GGCCAAAGGG GCCGTCCAGG 
     2641 GGGGCCTGGA CACCACCAAG TCTGTGGTCA TAGGTACAAA AGACACGATG TCCACTGGGC 
     2701 TCACGGGGGC AGCGAATGTG GCCAAGGGGG CTGTCCAGAC AGGTGTAGAC ACAGCCAAGA 
     2761 CCGTGCTGAC CGGCACCAAG GACACAGTGA CTACTGGGCT CGTGGGGGCA GTGAATGTCG 
     2821 CCAAAGGGAC CGTCCAGACA GGCATGGACA CCACCAAAAC TGTCCTAACC GGTACCAAGG 
     2881 ACACCATCTA CAGTGGGGTC ACCAGTGCCG TGAACGTGGC CAAGGGGGCT GTGCAAACTG 
     2941 GGCTGAAAAC GACCCAAAAT ATCGCGACAG GTACAAAGAA CACCTTTGGC AGTGGGGTGA 
     3001 CCAGTGCTGT GAATGTGGCC AAAGGGGCTG CCCAGACAGG TGTAGACACG GCCAAGACCG 
     3061 TGCTGACCGG CACCAAGGAC ACAGTCACTA CTGGGCTCAT GGGGGCAGTG AATGTCGCCA 
     3121 AAGGGACTGT CCAGACCAGT GTGGACACCA CCAAGACTGT CCTAACTGGT ACCAAGGACA 
     3181 CCGTCTGCAG TGGGGTGACC GGTGCTGCGA ATGTGGCCAA AGGGGCCATC CAAGGGGGCC 
     3241 TGGACACTAC AAAGTCTGTC CTGACTGGCA CTAAAGATGC TGTGTCCACT GGGCTCACAG 
     3301 GGGCTGTGAA GTTGGCCAAA GGGACTGTCC AGACCGGCAT GGACACCACC AAGACTGTGT 
     3361 TAACTGGTAC CAAGGATGCT GTGTGCAGTG GGGTGACCGG TGCTGCGAAT GTGGCCAAGG 
     3421 GGGCCGTCCA GATGGGTGTA GACACGGCCA AGACCGTGCT GACCGGTACC AAGGACACTG 
     3481 TCTGCAGTGG GGTCACCGGT GCTGCGAACG TGGCCAAGGG TGCTGTGCAA ACTGGGCTGA 
     3541 AAACGACCCA AAATATCGCA ACAGGTACAA AGAACACCCT TGGCAGTGGG GTGACCGGTG 
     3601 CTGCGAAAGT GGCCAAAGGG GCCGTCCAGG GGGGCCTGGA CACTACAAAG TCTGTCCTGA 
     3661 CTGGCACTAA AGATGCCGTG TCCACTGGGC TCACAGGGGC TGTGAACTTG GCCAAAGGGA 
     3721 CTGTCCAGAC CGGCGTGGAC ACCAGCAAGA CTGTCCTGAC CGGTACCAAG GACACCGTCT 
     3781 GCAGTGGAGT CACTGGTGCC GTAAATGTGG CCAAAGGGAC CGTCCAGACA GGTGTGGACA 
     3841 CAGCCAAGAC GGTGCTGAGT GGCGCTAAGG ATGCAGTGAC TACTGGAGTC ACGGGGGCAG 
     3901 TGAATGTGGC CAAAGGAACC GTGCAGACCG GCGTGGACGC CTCCAAGGCT GTGCTTATGG 
     3961 GTACCAAGGA CACTGTCTTC AGTGGGGTTA CCGGTGCCAT GAGCATGGCC AAAGGGGCCG 
     4021 TCCAGGGGGG CCTGGACACC ACCAAGACAG TGCTGACCGG AACCAAAGAC GCAGTGTCCG 
     4081 CTGGGCTCAT GGGGTCAGGG AACGTGGCGA CAGGGGCCAC CCACACTGGC CTCAGCACCT 
     4141 TCCAGAACTG GTTACCTAGT ACCCCCGCCA CCTCCTGGGG TGGACTCACC AGTTCCAGGA 
     4201 CCACAGACAA TGGTGGGGAG CAGACTGCCC TGAGCCCCCA AGAGGCCCCG TTCTCTGGCA 
     4261 TCTCCACGCC CCCGGATGTG CTCAGTGTAG GCCCGGAGCC TGCCTGGGAA GCCGCAGCCA 
     4321 CTACCAAGGG CCTTGCGACT GACGTGGCGA CGTTCACCCA AGGGGCCGCC CCAGGCAGGG 
     4381 AGGACACGGG GCTTTTGGCC ACCACACACG GCCCCGAAGA AGCCCCACGC TTGGCAATGC 
     4441 TGCAGAATGA GTTGGAGGGG CTGGGGGACA TCTTCCACCC CATGAATGCG GAGGAGCAAG 
     4501 CTCAGCTGGC TGCCTCCCAG CCTGGGCCAA AGGTGCTGTC GGCGGAACAG GGGAGCTACT 
     4561 TCGTTCGTTT AGGTGACCTG GGTCCCAGCT TCCGCCAGCG GGCATTTGAA CACGCGGTGA 
     4621 GCCACCTGCA GCACGGCCAG TTCCAAGCCA GGGACACTCT GGCCCAGCTC CAGGACTGCT 
     4681 TCAGGCTGAT TGAAAAGGCC CAGCAGGCTC CAGAAGGGCA GCCACGTCTG GACCAGGGCT 
     4741 CAGGTGCCAG TGCGGAGGAC GCTGCTGTCC AGGAGGAGCG GGATGCCGGG GTTCTGTCCA 
     4801 GGGTCTGCGG CCTTCTCCGG CAGCTGCACA CGGCCTACAG TGGCCTGGTC TCCAGCCTCC 
     4861 AGGGCCTGCC CGCCGAGCTC CAGCAGCCAG TGGGGCGGGC GCGGCACAGC CTCTGTGAGC 
     4921 TCTATGGCAT CGTGGCCTCA GCTGGCTCTG TAGAGGAGCT GCCCGCAGAG CGGCTGGTGC 
     4981 AGAGCCGCGA GGGTGTGCAC CAGGCTTGGC AGGGGTTAGA GCAGCTGCTG GAGGGCCTAC 
     5041 AGCACAATCC CCCGCTCAGC TGGCTGGTAG GGCCCTTCGC CTTGCCCGCT GGCGGGCAGA 
     5101 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5161 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5221 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5281 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5341 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5401 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5461 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5521 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5581 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5641 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5701 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5761 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5821 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5881 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5941 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6001 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6061 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6121 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6181 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6241 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6301 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6361 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6421 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6481 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6541 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6601 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6661 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6721 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6781 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6841 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6901 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6961 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7021 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7081 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7141 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7201 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7261 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7321 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7381 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7441 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7501 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7561 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7621 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7681 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7741 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7801 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7861 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7921 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7981 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8041 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8101 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8161 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8221 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8281 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8341 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8401 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8461 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8521 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8581 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8641 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8701 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8761 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8821 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8881 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8941 AACGCGAATT TT
//