LOCUS       RC224384L1                9213 bp    DNA     circular  SYN 
DEFINITION  ITIH2 (Myc-DDK-tagged)-Human inter-alpha (globulin) inhibitor H2 (ITIH2)
ACCESSION   NM_002216
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7504..7684 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8412..9071 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7843..8411 
                     /label="ColE1"  
     misc_feature    6379..6399 
                     /label="LR50 primer"  
     misc_feature    6176..6247 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6388..6979 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6158..6164 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6158 
                     /label="ITIH2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAAAAGACTC ACGTGCTTTT TCATCTGCTT 
     3361 CTTTCTTTCT GAAGTATCAG GCTTCGAAAT CCCCATAAAT GGACTTTCTG AATTTGTAGA 
     3421 CTATGAAGAT CTTGTGGAAC TGGCCCCAGG CAAATTTCAA TTGGTGGCAG AGAACCGGAG 
     3481 ATATCAGAGA AGCCTTCCAG GAGAATCGGA AGAAATGATG GAAGAGGTTG ATCAAGTAAC 
     3541 TCTTTATAGC TATAAAGTCC AGTCTACTAT TACTTCTCGG ATGGCCACCA CCATGATCCA 
     3601 GAGCAAAGTG GTGAACAATT CCCCGCAGCC TCAGAATGTC GTGTTTGATG TTCAGATCCC 
     3661 CAAAGGAGCA TTCATTTCCA ACTTCTCCAT GACTGTGGAC GGCAAGACAT TTAGGAGCTC 
     3721 TATTAAGGAG AAAACTGTGG GCCGAGCTCT TTATGCACAG GCCAGAGCAA AAGGCAAGAC 
     3781 GGCTGGCTTG GTGAGGAGCA GCGCTCTTGA TATGGAAAAC TTCAGAACGG AAGTAAATGT 
     3841 CCTCCCAGGA GCAAAGGTGC AGTTCGAACT TCACTACCAG GAGGTGAAGT GGAGGAAGCT 
     3901 GGGCTCCTAT GAGCACAGGA TCTATCTGCA ACCTGGACGG CTGGCCAAAC ACTTAGAGGT 
     3961 AGATGTGTGG GTTATCGAAC CACAGGGACT GAGATTTCTT CATGTTCCCG ACACATTTGA 
     4021 AGGCCATTTC GATGGTGTTC CGGTCATTTC TAAAGGACAA CAGAAGGCGC ACGTCTCCTT 
     4081 CAAGCCCACG GTAGCACAGC AGAGAATATG CCCTAGCTGC CGGGAGACTG CGGTAGATGG 
     4141 GGAACTGGTG GTGCTGTATG ACGTGAAAAG AGAAGAGAAG GCTGGTGAAC TGGAGGTGTT 
     4201 TAATGGATAT TTTGTCCACT TCTTTGCTCC TGACAACCTG GACCCAATTC CCAAAAACAT 
     4261 CCTCTTTGTC ATCGATGTGA GTGGCTCCAT GTGGGGAGTT AAAATGAAAC AAACTGTGGA 
     4321 AGCAATGAAG ACCATATTGG ATGACCTCAG AGCAGAAGAC CATTTCTCTG TGATTGATTT 
     4381 CAACCAGAAC ATTCGAACTT GGAGAAATGA TTTAATTTCA GCTACAAAAA CACAGGTTGC 
     4441 AGATGCCAAG AGGTATATTG AGAAAATCCA GCCCAGTGGA GGCACAAACA TCAACGAAGC 
     4501 ACTCCTACGG GCAATCTTCA TTTTGAATGA AGCCAATAAC TTGGGACTGT TAGACCCCAA 
     4561 CTCCGTCTCG CTGATCATTT TGGTTTCTGA TGGAGATCCA ACAGTGGGCG AACTAAAACT 
     4621 GTCAAAAATT CAGAAAAACG TTAAGGAGAA CATCCAAGAC AATATCTCCT TGTTCAGTTT 
     4681 GGGCATGGGA TTTGATGTGG ACTATGATTT TTTGAAGAGA CTGTCCAATG AAAACCATGG 
     4741 AATTGCACAA AGGATTTATG GAAACCAGGA CACGTCTTCC CAGCTTAAGA AATTCTACAA 
     4801 CCAGGTCTCC ACTCCATTGC TCCGGAATGT TCAGTTCAAC TATCCCCATA CATCAGTCAC 
     4861 GGACGTCACT CAAAACAATT TCCATAACTA CTTTGGAGGC TCAGAGATTG TGGTGGCAGG 
     4921 AAAATTTGAC CCTGCTAAAT TGGATCAAAT AGAGAGCGTT ATCACGGCGA CTTCGGCTAA 
     4981 CACGCAGTTA GTCTTGGAGA CCCTGGCCCA GATGGACGAC TTGCAGGATT TTCTATCGAA 
     5041 AGACAAGCAT GCAGATCCCG ATTTCACCAG GAAACTGTGG GCCTATCTAA CCATCAACCA 
     5101 ACTGCTAGCT GAACGAAGCC TGGCTCCTAC AGCTGCCGCC AAGAGAAGAA TTACAAGATC 
     5161 GATCCTGCAG ATGTCTCTAG ACCACCACAT TGTGACTCCG CTGACCTCGC TGGTGATCGA 
     5221 GAACGAGGCT GGGGATGAGC GCATGCTGGC GGATGCCCCA CCGCAGGATC CCTCCTGCTG 
     5281 CTCAGGGGCC CTGTATTACG GCAGCAAAGT GGTTCCAGAT TCCACCCCGT CTTGGGCCAA 
     5341 TCCTTCACCA ACGCCCGTGA TCTCCATGCT GGCACAAGGA TCTCAGGTGC TAGAGTCCAC 
     5401 GCCACCCCCA CATGTGATGA GAGTTGAAAA TGACCCACAT TTCATCATTT ATCTACCAAA 
     5461 AAGCCAAAAG AACATTTGTT TCAATATTGA CTCAGAACCT GGAAAAATCC TCAACCTGGT 
     5521 TTCTGACCCA GAATCAGGAA TTGTAGTCAA CGGTCAGCTT GTTGGTGCCA AGAAGCCCAA 
     5581 CAATGGAAAA CTAAGCACCT ATTTTGGAAA ACTGGGATTT TATTTCCAAA GTGAAGACAT 
     5641 AAAAATAGAA ATCAGCACTG AGACCATCAC CCTGAGCCAT GGTTCTAGCA CATTCTCCTT 
     5701 GTCCTGGTCC GACACGGCTC AAGTCACGAA TCAGAGGGTG CAGATCTCAG TGAAGAAAGA 
     5761 AAAAGTGGTA ACTATCACCC TGGATAAAGA GATGTCCTTT TCTGTTTTAC TTCATCGTGT 
     5821 TTGGAAGAAG CATCCCGTCA ATGTTGACTT TCTGGGAATC TACATACCCC CTACAAACAA 
     5881 GTTCTCACCT AAAGCCCACG GACTAATAGG CCAGTTCATG CAGGAACCAA AGATACACAT 
     5941 CTTCAATGAG AGACCAGGAA AGGACCCTGA GAAGCCAGAG GCCAGCATGG AAGTGAAGGG 
     6001 GCAGAAGCTG ATCATCACCA GGGGCTTACA GAAAGACTAC AGAACGGATC TAGTGTTTGG 
     6061 AACGGACGTT ACCTGCTGGT TTGTGCACAA CAGTGGAAAA GGATTCATTG ACGGGCATTA 
     6121 CAAGGATTAC TTCGTGCCTC AGCTCTACAG CTTTCTCAAA CGGCCTACGC GTACGCGGCC 
     6181 GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA 
     6241 GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG 
     6301 GGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG 
     6361 TTCCACTAGG TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 
     6421 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 
     6481 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 
     6541 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 
     6601 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 
     6661 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 
     6721 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 
     6781 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 
     6841 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 
     6901 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 
     6961 ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA 
     7021 ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA 
     7081 AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT 
     7141 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG 
     7201 GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG 
     7261 CTACTTCCCT GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT 
     7321 TGGATGGTGC TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG 
     7381 AGAGAACACC CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA 
     7441 AGTATTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA 
     7501 TCCGGACTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA 
     7561 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG 
     7621 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG 
     7681 GAAAATCTCT AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     7741 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     7801 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     7861 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     7921 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     7981 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     8041 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     8101 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     8161 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     8221 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     8281 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     8341 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     8401 TTAAGGGATT TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT 
     8461 TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC 
     8521 AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG 
     8581 AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG 
     8641 GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG 
     8701 TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA 
     8761 CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA 
     8821 CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC 
     8881 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG 
     8941 GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT 
     9001 GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT 
     9061 CCAGTGATTT TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     9121 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTC 
     9181 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAC
//