LOCUS       RC224384                7719 bp    DNA     circular  SYN 
DEFINITION  ITIH2 (Myc-DDK-tagged)-Human inter-alpha (globulin) inhibitor H2 (ITIH2)
ACCESSION   NM_002216
VERSION     NM_002216.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3876..3967 
                     /label="Myc-DDK"  
     misc_feature    3997..4582 
                     /label="PolyA signal"  
     misc_feature    4054..4073 
                     /label="Restriction Enzyme"  
     misc_feature    4858..5530 
                     /label="ColE1"  
     misc_feature    5960..6754 
                     /label="Kan/Neo"  
     misc_feature    6789..7091 
                     /label="SV40 ori"  
     misc_feature    7177..7654 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3858..3864 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3858 
                     /label="ITIH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAAGACTC ACGTGCTTTT TCATCTGCTT CTTTCTTTCT GAAGTATCAG 
     1081 GCTTCGAAAT CCCCATAAAT GGACTTTCTG AATTTGTAGA CTATGAAGAT CTTGTGGAAC 
     1141 TGGCCCCAGG CAAATTTCAA TTGGTGGCAG AGAACCGGAG ATATCAGAGA AGCCTTCCAG 
     1201 GAGAATCGGA AGAAATGATG GAAGAGGTTG ATCAAGTAAC TCTTTATAGC TATAAAGTCC 
     1261 AGTCTACTAT TACTTCTCGG ATGGCCACCA CCATGATCCA GAGCAAAGTG GTGAACAATT 
     1321 CCCCGCAGCC TCAGAATGTC GTGTTTGATG TTCAGATCCC CAAAGGAGCA TTCATTTCCA 
     1381 ACTTCTCCAT GACTGTGGAC GGCAAGACAT TTAGGAGCTC TATTAAGGAG AAAACTGTGG 
     1441 GCCGAGCTCT TTATGCACAG GCCAGAGCAA AAGGCAAGAC GGCTGGCTTG GTGAGGAGCA 
     1501 GCGCTCTTGA TATGGAAAAC TTCAGAACGG AAGTAAATGT CCTCCCAGGA GCAAAGGTGC 
     1561 AGTTCGAACT TCACTACCAG GAGGTGAAGT GGAGGAAGCT GGGCTCCTAT GAGCACAGGA 
     1621 TCTATCTGCA ACCTGGACGG CTGGCCAAAC ACTTAGAGGT AGATGTGTGG GTTATCGAAC 
     1681 CACAGGGACT GAGATTTCTT CATGTTCCCG ACACATTTGA AGGCCATTTC GATGGTGTTC 
     1741 CGGTCATTTC TAAAGGACAA CAGAAGGCGC ACGTCTCCTT CAAGCCCACG GTAGCACAGC 
     1801 AGAGAATATG CCCTAGCTGC CGGGAGACTG CGGTAGATGG GGAACTGGTG GTGCTGTATG 
     1861 ACGTGAAAAG AGAAGAGAAG GCTGGTGAAC TGGAGGTGTT TAATGGATAT TTTGTCCACT 
     1921 TCTTTGCTCC TGACAACCTG GACCCAATTC CCAAAAACAT CCTCTTTGTC ATCGATGTGA 
     1981 GTGGCTCCAT GTGGGGAGTT AAAATGAAAC AAACTGTGGA AGCAATGAAG ACCATATTGG 
     2041 ATGACCTCAG AGCAGAAGAC CATTTCTCTG TGATTGATTT CAACCAGAAC ATTCGAACTT 
     2101 GGAGAAATGA TTTAATTTCA GCTACAAAAA CACAGGTTGC AGATGCCAAG AGGTATATTG 
     2161 AGAAAATCCA GCCCAGTGGA GGCACAAACA TCAACGAAGC ACTCCTACGG GCAATCTTCA 
     2221 TTTTGAATGA AGCCAATAAC TTGGGACTGT TAGACCCCAA CTCCGTCTCG CTGATCATTT 
     2281 TGGTTTCTGA TGGAGATCCA ACAGTGGGCG AACTAAAACT GTCAAAAATT CAGAAAAACG 
     2341 TTAAGGAGAA CATCCAAGAC AATATCTCCT TGTTCAGTTT GGGCATGGGA TTTGATGTGG 
     2401 ACTATGATTT TTTGAAGAGA CTGTCCAATG AAAACCATGG AATTGCACAA AGGATTTATG 
     2461 GAAACCAGGA CACGTCTTCC CAGCTTAAGA AATTCTACAA CCAGGTCTCC ACTCCATTGC 
     2521 TCCGGAATGT TCAGTTCAAC TATCCCCATA CATCAGTCAC GGACGTCACT CAAAACAATT 
     2581 TCCATAACTA CTTTGGAGGC TCAGAGATTG TGGTGGCAGG AAAATTTGAC CCTGCTAAAT 
     2641 TGGATCAAAT AGAGAGCGTT ATCACGGCGA CTTCGGCTAA CACGCAGTTA GTCTTGGAGA 
     2701 CCCTGGCCCA GATGGACGAC TTGCAGGATT TTCTATCGAA AGACAAGCAT GCAGATCCCG 
     2761 ATTTCACCAG GAAACTGTGG GCCTATCTAA CCATCAACCA ACTGCTAGCT GAACGAAGCC 
     2821 TGGCTCCTAC AGCTGCCGCC AAGAGAAGAA TTACAAGATC GATCCTGCAG ATGTCTCTAG 
     2881 ACCACCACAT TGTGACTCCG CTGACCTCGC TGGTGATCGA GAACGAGGCT GGGGATGAGC 
     2941 GCATGCTGGC GGATGCCCCA CCGCAGGATC CCTCCTGCTG CTCAGGGGCC CTGTATTACG 
     3001 GCAGCAAAGT GGTTCCAGAT TCCACCCCGT CTTGGGCCAA TCCTTCACCA ACGCCCGTGA 
     3061 TCTCCATGCT GGCACAAGGA TCTCAGGTGC TAGAGTCCAC GCCACCCCCA CATGTGATGA 
     3121 GAGTTGAAAA TGACCCACAT TTCATCATTT ATCTACCAAA AAGCCAAAAG AACATTTGTT 
     3181 TCAATATTGA CTCAGAACCT GGAAAAATCC TCAACCTGGT TTCTGACCCA GAATCAGGAA 
     3241 TTGTAGTCAA CGGTCAGCTT GTTGGTGCCA AGAAGCCCAA CAATGGAAAA CTAAGCACCT 
     3301 ATTTTGGAAA ACTGGGATTT TATTTCCAAA GTGAAGACAT AAAAATAGAA ATCAGCACTG 
     3361 AGACCATCAC CCTGAGCCAT GGTTCTAGCA CATTCTCCTT GTCCTGGTCC GACACGGCTC 
     3421 AAGTCACGAA TCAGAGGGTG CAGATCTCAG TGAAGAAAGA AAAAGTGGTA ACTATCACCC 
     3481 TGGATAAAGA GATGTCCTTT TCTGTTTTAC TTCATCGTGT TTGGAAGAAG CATCCCGTCA 
     3541 ATGTTGACTT TCTGGGAATC TACATACCCC CTACAAACAA GTTCTCACCT AAAGCCCACG 
     3601 GACTAATAGG CCAGTTCATG CAGGAACCAA AGATACACAT CTTCAATGAG AGACCAGGAA 
     3661 AGGACCCTGA GAAGCCAGAG GCCAGCATGG AAGTGAAGGG GCAGAAGCTG ATCATCACCA 
     3721 GGGGCTTACA GAAAGACTAC AGAACGGATC TAGTGTTTGG AACGGACGTT ACCTGCTGGT 
     3781 TTGTGCACAA CAGTGGAAAA GGATTCATTG ACGGGCATTA CAAGGATTAC TTCGTGCCTC 
     3841 AGCTCTACAG CTTTCTCAAA CGGCCTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3901 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3961 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4021 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4081 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4141 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4201 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4261 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4321 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4381 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4441 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4501 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4561 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4621 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4681 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4741 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4801 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4861 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4921 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4981 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5041 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5101 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5161 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5221 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5281 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5341 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5401 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5461 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5521 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5581 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5641 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5701 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5761 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5821 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5881 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5941 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6001 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6061 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6121 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6181 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6241 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6301 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6361 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6421 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6481 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6541 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6601 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6661 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6721 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6781 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6841 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6901 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6961 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7021 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7081 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7141 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7201 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7261 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7321 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7381 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7441 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7501 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7561 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7621 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7681 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//