LOCUS       RC224345                6894 bp    DNA     circular  SYN 
DEFINITION  TCF4 (Myc-DDK-tagged)-Human transcription factor 4 (TCF4), transcript variant 1
ACCESSION   NM_001083962
VERSION     NM_001083962.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3051..3142 
                     /label="Myc-DDK"  
     misc_feature    3172..3757 
                     /label="PolyA signal"  
     misc_feature    3229..3248 
                     /label="Restriction Enzyme"  
     misc_feature    4033..4705 
                     /label="ColE1"  
     misc_feature    5135..5929 
                     /label="Kan/Neo"  
     misc_feature    5964..6266 
                     /label="SV40 ori"  
     misc_feature    6352..6829 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3033..3039 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3033 
                     /label="TCF4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATCACCAA CAGCGAATGG CTGCCTTAGG GACGGACAAA GAGCTGAGTG 
     1081 ATTTACTGGA TTTCAGTGCG ATGTTTTCAC CTCCTGTGAG CAGTGGGAAA AATGGACCAA 
     1141 CTTCTTTGGC AAGTGGACAT TTTACTGGCT CAAATGTAGA AGACAGAAGT AGCTCAGGGT 
     1201 CCTGGGGGAA TGGAGGACAT CCAAGCCCGT CCAGGAACTA TGGAGATGGG ACTCCCTATG 
     1261 ACCACATGAC CAGCAGGGAC CTTGGGTCAC ATGACAATCT CTCTCCACCT TTTGTCAATT 
     1321 CCAGAATACA AAGTAAAACA GAAAGGGGCT CATACTCATC TTATGGGAGA GAATCAAACT 
     1381 TACAGGGTTG CCACCAGCAG AGTCTCCTTG GAGGTGACAT GGATATGGGC AACCCAGGAA 
     1441 CCCTTTCGCC CACCAAACCT GGTTCCCAGT ACTATCAGTA TTCTAGCAAT AATCCCCGAA 
     1501 GGAGGCCTCT TCACAGTAGT GCCATGGAGG TACAGACAAA GAAAGTTCGA AAAGTTCCTC 
     1561 CAGGTTTGCC ATCTTCAGTC TATGCTCCAT CAGCAAGCAC TGCCGACTAC AATAGGGACT 
     1621 CGCCAGGCTA TCCTTCCTCC AAACCAGCAA CCAGCACTTT CCCTAGCTCC TTCTTCATGC 
     1681 AAGATGGCCA TCACAGCAGT GACCCTTGGA GCTCCTCCAG TGGGATGAAT CAGCCTGGCT 
     1741 ATGCAGGAAT GTTGGGCAAC TCTTCTCATA TTCCACAGTC CAGCAGCTAC TGTAGCCTGC 
     1801 ATCCACATGA ACGTTTGAGC TATCCATCAC ACTCCTCAGC AGACATCAAT TCCAGTCTTC 
     1861 CTCCGATGTC CACTTTCCAT CGTAGTGGTA CAAACCATTA CAGCACCTCT TCCTGTACGC 
     1921 CTCCTGCCAA CGGGACAGAC AGTATAATGG CAAATAGAGG AAGCGGGGCA GCCGGCAGCT 
     1981 CCCAGACTGG AGATGCTCTG GGGAAAGCAC TTGCTTCGAT CTATTCTCCA GATCACACTA 
     2041 ACAACAGCTT TTCATCAAAC CCTTCAACTC CTGTTGGCTC TCCTCCATCT CTCTCAGCAG 
     2101 GCACAGCTGT TTGGTCTAGA AATGGAGGAC AGGCCTCATC GTCTCCTAAT TATGAAGGAC 
     2161 CCTTACACTC TTTGCAAAGC CGAATTGAAG ATCGTTTAGA AAGACTGGAT GATGCTATTC 
     2221 ATGTTCTCCG GAACCATGCA GTGGGCCCAT CCACAGCTAT GCCTGGTGGT CATGGGGACA 
     2281 TGCATGGAAT CATTGGACCT TCTCATAATG GAGCCATGGG TGGTCTGGGC TCAGGGTATG 
     2341 GAACCGGCCT TCTTTCAGCC AACAGACATT CACTCATGGT GGGGACCCAT CGTGAAGATG 
     2401 GCGTGGCCCT GAGAGGCAGC CATTCTCTTC TGCCAAACCA GGTTCCGGTT CCACAGCTTC 
     2461 CTGTCCAGTC TGCGACTTCC CCTGACCTGA ACCCACCCCA GGACCCTTAC AGAGGCATGC 
     2521 CACCAGGACT ACAGGGGCAG AGTGTCTCCT CTGGCAGCTC TGAGATCAAA TCCGATGACG 
     2581 AGGGTGATGA GAACCTGCAA GACACGAAAT CTTCGGAGGA CAAGAAATTA GATGACGACA 
     2641 AGAAGGATAT CAAATCAATT ACTAGGTCAA GATCTAGCAA TAATGACGAT GAGGACCTGA 
     2701 CACCAGAGCA GAAGGCAGAG CGTGAGAAGG AGCGGAGGAT GGCCAACAAT GCCCGAGAGC 
     2761 GTCTGCGGGT CCGTGACATC AACGAGGCTT TCAAAGAGCT CGGCCGCATG GTGCAGCTCC 
     2821 ACCTCAAGAG TGACAAGCCC CAGACCAAGC TCCTGATCCT CCACCAGGCG GTGGCCGTCA 
     2881 TCCTCAGTCT GGAGCAGCAA GTCCGAGAAA GGAATCTGAA TCCGAAAGCT GCGTGTCTGA 
     2941 AAAGAAGGGA GGAAGAGAAG GTGTCCTCGG AGCCTCCCCC TCTCTCCTTG GCCGGCCCAC 
     3001 ACCCTGGAAT GGGAGACGCA TCGAATCACA TGGGACAGAT GACGCGTACG CGGCCGCTCG 
     3061 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3121 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3181 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3241 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3301 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3361 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3421 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3481 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3541 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3601 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3661 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     3721 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     3781 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     3841 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     3901 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     3961 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4021 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4081 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4141 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4201 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4261 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4321 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4381 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4441 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4501 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4561 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4621 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4681 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     4741 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     4801 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     4861 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     4921 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     4981 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5041 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5101 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5161 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5221 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5281 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5341 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5401 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5461 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5521 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5581 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5641 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     5701 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     5761 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     5821 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     5881 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     5941 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6001 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6061 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6121 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6181 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6241 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6301 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6361 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6421 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6481 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6541 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6601 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6661 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     6721 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     6781 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     6841 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//