LOCUS       RC224312                7677 bp    DNA     circular  SYN 
DEFINITION  PCDHGA8 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 8 (PCDHGA8), transcript variant 1
ACCESSION   NM_032088
VERSION     NM_032088.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3834..3925 
                     /label="Myc-DDK"  
     misc_feature    3955..4540 
                     /label="PolyA signal"  
     misc_feature    4012..4031 
                     /label="Restriction Enzyme"  
     misc_feature    4816..5488 
                     /label="ColE1"  
     misc_feature    5918..6712 
                     /label="Kan/Neo"  
     misc_feature    6747..7049 
                     /label="SV40 ori"  
     misc_feature    7135..7612 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3816..3822 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3816 
                     /label="PCDHGA8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCTCCA CAGAGTCGCC CCAGACGCGG CGAGCTGATC CTGCTGTGCG 
     1081 CGCTGCTGGG AACGCTGTGG GAAATCGGGA GGGGACAGAT TCGCTACTCT GTGCCAGAAG 
     1141 AGACGGACAA AGGCTCCTTC GTGGGTAATA TCTCCAAGGA CCTGGGGCTG GACCCCCGGA 
     1201 AGCTGGCGAA GCACGGAGTC CGTATCGTCT CCAGAGGTAG GACGCAGCTC TTTGCTCTGA 
     1261 ACCCGCGCAG CGGCAGCTTG ATCACCGCGG GCAGGATAGA TCGGGAGGAG CTCTGCGCTC 
     1321 AGAGCCCGCG GTGTCTGATA AATATTAACA CCCTGGTTGA GGATAAAGGA AAACTCTTTG 
     1381 GGGTAGAAAT AGAAATAATT GATATTAACG ATAATAACCC AAAATTCCAG GTCGAAGATC 
     1441 TAGAAGTAAA AATTAACGAA ATCGCGGTTC CTGGAGCACG TTATCCACTC CCAGAAGCTG 
     1501 TTGACCCGGA TGTGGGCGTG AACTCCCTCC AGAGCTACCA GCTCAGCCCC AATCACCACT 
     1561 TCTCCCTGGA CGTGCAGACT GGAGACAATG GAGCCATAAA CCCAGAGCTG GTGCTGGAGC 
     1621 GCGCCCTGGA CAGGGAGGAA GAGGCTGCTC ACCACCTGGT CCTCACGGCC TCGGATGGCG 
     1681 GCAAGCCGCC TCGCTCTAGC ACAGTGCGCA TCCACGTGAC AGTGTTGGAT ACAAATGACA 
     1741 ATGCCCCGGT TTTTCCTCAC CCGATTTACC GAGTGAAAGT CCTTGAGAAC ATGCCCCCAG 
     1801 GCACGCGGCT GCTTACTGTA ACAGCCAGCG ACCCGGATGA GGGAATCAAC GGAAAAGTGG 
     1861 CATACAAATT CCGGAAAATT AATGAAAAAC AAACTCCGTT ATTCCAGCTT AATGAAAATA 
     1921 CTGGGGAAAT ATCAATAGCA AAAAGTCTAG ATTATGAAGA ATGTTCATTT TATGAAATGG 
     1981 AAATACAAGC CGAAGATGTG GGGGCACTTC TGGGGAGGAC CAAATTGCTC ATTTCGGTGG 
     2041 AAGATGTAAA TGACAATAGA CCAGAAGTGA TCATTACGTC TTTGTTTAGC CCAGTGTTAG 
     2101 AAAATTCTCT TCCCGGGACA GTAATTGCCT TCTTGAGTGT GCATGACCAA GACTCTGGAA 
     2161 AGAATGGTCA AGTTGTCTGT TACACACGTG ATAATTTACC TTTTAAATTA GAAAAGTCAA 
     2221 TAGGTAATTA TTATAGATTA GTGACAAGGA AATATTTGGA CCGAGAAAAT GTCTCTATCT 
     2281 ACAATATCAC AGTGATGGCC TCAGATCTAG GAACACCACC TCTGTCCACT GAAACTCAAA 
     2341 TCGCTCTGCA CGTGGCAGAC ATTAACGACA ACCCTCCTAC TTTCCCTCAT GCCTCCTACT 
     2401 CAGCGTATAT CCTAGAGAAC AACCTGAGAG GAGCCTCCAT CTTTTCCTTG ACTGCACACG 
     2461 ACCCCGACAG CCAGGAGAAT GCCCAGGTCA CTTACTCTGT GACCGAGGAC ACGCTGCAGG 
     2521 GGGCGCCCCT GTCCTCGTAT ATCTCCATCA ACTCTGACAC CGGTGTCCTG TATGCGCTGC 
     2581 AATCTTTCGA CTATGAGCAG ATCCGAGACC TGCAGCTACT GGTAACAGCC AGCGACAGCG 
     2641 GGGACCCGCC CCTCAGCAGC AACATGTCAC TGAGCCTGTT CGTGCTGGAC CAGAATGACA 
     2701 ACGCGCCCGA GATCCTGTAC CCCGCCCTCC CCACAGACGG TTCCACTGGC GTGGAGCTGG 
     2761 CGCCCCGCTC CGCAGAGCGT GGCTACCTGG TGACCAAGGT GGTGGCGGTG GACAGAGACT 
     2821 CGGGCCAGAA CGCCTGGCTG TCCTACCGCC TGCTCAAGGC CAGCGAGCCG GGACTCTTCT 
     2881 CGGTGGGTCT GCACACGGGC GAGGTGCGCA CGGCGCGAGC CCTGCTGGAC AGAGATGCGC 
     2941 TCAAGCAGAG CCTCGTGGTG GCCGTCCAGG ACCATGGCCA GCCCCCTCTC TCCGCCACTG 
     3001 TCACGCTCAC CGTAGCCGTG GCTGACAGCA TCCCCGAAGT CCTGACCGAG TTGGGCAGTC 
     3061 TGAAGCCTTC GGTCGACCCG AACGATTCGA GCCTTACACT CTATCTCGTG GTGGCAGTGG 
     3121 CTGCCATCTC CTGTGTCTTC CTCGCCTTTG TCGCTGTGCT TCTGGGGCTC AGGCTGAGGC 
     3181 GCTGGCACAA GTCACGCCTG CTCCAGGATT CCGGTGGCAG ATTGGTAGGC GTGCCTGCCT 
     3241 CACATTTTGT GGGTGTTGAG GAGGTACAGG CTTTCCTGCA GACCTATTCC CAGGAAGTCT 
     3301 CCCTCACCGC CGACTCGCGG AAGAGTCACC TGATCTTTCC CCAGCCCAAC TACGCAGACA 
     3361 TGCTCATCAG TCAGGAGGGC TGTGAGAAAA ATGATTCTTT GTTAACATCC GTAGATTTTC 
     3421 ATGAATATAA GAATGAAGCT GATCATGGTC AGCAAGCCCC GCCCAACACG GACTGGCGTT 
     3481 TCTCTCAGGC CCAGAGACCC GGCACCAGCG GCTCCCAAAA TGGCGATGAC ACCGGCACCT 
     3541 GGCCCAACAA CCAGTTTGAC ACAGAGATGC TGCAAGCCAT GATCTTGGCG TCCGCCAGTG 
     3601 AAGCTGCTGA TGGGAGCTCC ACCCTGGGAG GGGGTGCCGG CACCATGGGA TTGAGCGCCC 
     3661 GCTACGGACC CCAGTTCACC CTGCAGCACG TGCCCGACTA CCGCCAGAAT GTCTACATCC 
     3721 CAGGCAGCAA TGCCACACTG ACCAACGCAG CTGGCAAGCG GGATGGCAAG GCCCCAGCAG 
     3781 GTGGCAATGG CAACAAGAAG AAGTCGGGCA AGAAGGAGAA GAAGACGCGT ACGCGGCCGC 
     3841 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3901 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3961 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4021 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4081 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4141 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4201 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4261 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4321 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4381 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4441 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4501 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4561 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4621 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4681 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4741 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4801 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4861 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4921 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4981 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5041 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5101 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5161 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5221 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5281 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5341 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5401 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5461 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5521 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5581 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5641 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5701 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5761 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5821 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5881 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5941 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6001 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6061 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6121 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6181 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6241 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6301 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6361 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6421 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6481 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6541 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6601 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6661 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6721 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6781 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6841 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6901 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6961 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7021 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7081 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7141 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7201 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7261 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7321 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7381 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7441 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7501 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7561 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7621 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
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