LOCUS       RC224284                6843 bp    DNA     circular  SYN 
DEFINITION  MIA2 (Myc-DDK-tagged)-Human melanoma inhibitory activity 2 (MIA2)
ACCESSION   NM_054024
VERSION     NM_054024.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3000..3091 
                     /label="Myc-DDK"  
     misc_feature    3121..3706 
                     /label="PolyA signal"  
     misc_feature    3178..3197 
                     /label="Restriction Enzyme"  
     misc_feature    3982..4654 
                     /label="ColE1"  
     misc_feature    5084..5878 
                     /label="Kan/Neo"  
     misc_feature    5913..6215 
                     /label="SV40 ori"  
     misc_feature    6301..6778 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2982..2988 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2982 
                     /label="MIA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAAATTT GGCGTTCACA GAATCCTTCT TCTGGCTATT TCTCTGACAA 
     1081 AGTGTCTGGA GAGTACAAAA CTGCTGGCAG ACCTTAAAAA ATGTGGTGAC TTGGAATGTG 
     1141 AAGCTTTAAT AAACAGAGTC TCAGCCATGA GAGATTATAG AGGACCTGAC TGCCGATACC 
     1201 TGAACTTCAC TAAGGGAGAA GAGATATCTG TTTATGTTAA ACTTGCAGGA GAAAGGGAAG 
     1261 ATTTGTGGGC AGGAAGTAAA GGAAAGGAGT TTGGATATTT TCCCAGAGAT GCAGTCCAGA 
     1321 TTGAAGAGGT GTTCATATCT GAGGAAATTC AGATGTCAAC GAAAGAATCT GACTTTCTTT 
     1381 GTCTTCTTGG AGTAAGTTAC ACATTTGACA ATGAAGATAG TGAATTAAAC GGTGATTATG 
     1441 GTGAAAATAT ATATCCTTAT GAAGAAGATA AAGATGAAAA ATCTAGTATA TATGAAAGTG 
     1501 ATTTTCAGAT AGAACCTGGA TTTTATGCAA CTTATGAAAG TACTTTGTTT GAAGACCAAG 
     1561 TTCCAGCATT AGAGGCTCCT GAAGATATCG GAAGTACCAG TGAATCAAAA GACTGGGAAG 
     1621 AAGTAGTTGT TGAAAGTATG GAACAGGATC GTATTCCAGA AGTGCATGTC CCACCATCTT 
     1681 CAGCTGTGTC TGGAGTCAAA GAATGGTTTG GATTGGGAGG AGAACAAGCT GAAGAGAAGG 
     1741 CTTTTGAATC AGTTATTGAA CCTGTACAAG AAAGCTCATT TCGGAGTAGA AAAATAGCAG 
     1801 TGGAAGATGA GAATGACCTA GAGGAATTAA ATAATGGTGA GCCTCAAACA GAACATCAGC 
     1861 AAGAATCTGA ATCAGAAATT GATTCAGTGC CAAAGACACA GTCTGAACTA GCATCTGAGT 
     1921 CAGAGCACAT TCCCAAACCT CAATCCACTG GTTGGTTTGG TGGAGGATTT ACAAGTTATT 
     1981 TAGGTTTTGG AGATGAGGAT ACAGGGCTTG AATTAATAGC TGAAGAAAGC AATCCACCAC 
     2041 TACAAGATTT TCCCAATTCC ATATCATCTG ATAAAGAAGC CACAGTTCCA TGTACAGAAA 
     2101 TATTAACAGA AAAAAAAGAC ACAATCACTA ATGATAGCTT GAGTCTCAAG CCAAGTTGGT 
     2161 TTGATTTTGG TTTTGCTATA CTAGGCTTTG CATATGCCAA GGAAGATAAA ATTATGTTAG 
     2221 ATGACAGGAA AAATGAAGAA GATGGTGGGG CAGATGAACA TGAACATCCT CTAACAAGTG 
     2281 AATTAGACCC TGAAAAAGAA CAAGAAATAG AAACGATAAA AATTATAGAA ACAGAAGATC 
     2341 AAATAGACAA GAAACCAGTC TCAGAAAAAA CAGACGAATC TGATACTATA CCATATTTGA 
     2401 AAAAGTTCTT GTATAATTTT GACAACCCTT GGAACTTCCA GAACATTCCA AAGGAAACAG 
     2461 AATTGCCATT TCCCAAACAG ATACTGGATC AAAATAATGT AATTGAAAAT GAAGAAACTG 
     2521 GAGAATTTTC CATTGATAAT TATCCCACAG ATAATACAAA AGTTATGATA TTCAAAAGTT 
     2581 CATACAGTCT GTCAGATATG GTCTCTAACA TAGAGTTACC TACGAGAATT CACGAAGAAG 
     2641 TATATTTTGA ACCCTCATCT TCTAAAGATA GTGATGAAAA TTCGAAACCA TCAGTAGACA 
     2701 CCGAAGGGCC TGCTCTGGTG GAGATAGACA GATCTGTGGA AAATACCCTG CTAAATAGTC 
     2761 AGATGGTTTC AACTGATAAC TCTTTGTCTT CTCAAAATTA TATTTCTCAG AAAGAAGATG 
     2821 CTTCTGAGTT TCAGATTCTG AAATACTTAT TCCAAATTGA TGTTTATGAT TTCATGAATT 
     2881 CTGCATTTTC ACCAATTGTA ATTCTTACAG AAAGGGTAAG TTTGCCTTTT AAACCTTTTG 
     2941 CAATAATTTT ACCTATTTTG CTAAATATAA GAGTGGCTAC AAAATATGTT ACGCGTACGC 
     3001 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3061 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3121 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3181 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3241 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3301 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3361 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3421 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     3481 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     3541 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     3601 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     3661 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     3721 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     3781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     3841 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     3901 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     3961 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4021 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4081 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4141 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4201 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4261 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4321 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4381 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4441 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     4501 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     4561 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     4621 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     4681 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     4741 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     4801 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     4861 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     4921 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     4981 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5041 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5101 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5161 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5221 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5281 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5341 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5401 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     5461 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     5521 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     5581 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     5641 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     5701 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     5761 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     5821 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     5881 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     5941 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6001 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6061 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6121 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6181 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6241 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6301 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6361 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6421 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     6481 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     6541 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     6601 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     6661 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     6721 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     6781 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     6841 TTT
//