LOCUS       RC224249                7932 bp    DNA     circular  SYN 
DEFINITION  FAN1 (Myc-DDK-tagged)-Human FANCD2/FANCI-associated nuclease 1 (FAN1), transcript variant 1
ACCESSION   NM_014967
VERSION     NM_014967.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4089..4180 
                     /label="Myc-DDK"  
     misc_feature    4210..4795 
                     /label="PolyA signal"  
     misc_feature    4267..4286 
                     /label="Restriction Enzyme"  
     misc_feature    5071..5743 
                     /label="ColE1"  
     misc_feature    6173..6967 
                     /label="Kan/Neo"  
     misc_feature    7002..7304 
                     /label="SV40 ori"  
     misc_feature    7390..7867 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4071..4077 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4071 
                     /label="FAN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTCAGAA GGGAAACCTC CTGACAAAAA AAGGCCTCGT AGAAGCTTAT 
     1081 CAATCAGCAA GAATAAGAAA AAAGCATCTA ATTCTATTAT TTCGTGTTTT AACAATGCAC 
     1141 CACCTGCTAA ACTTGCCTGC CCCGTTTGCA GTAAAATGGT GCCTAGATAT GACTTAAACC 
     1201 GGCACCTTGA TGAAATGTGT GCTAACAATG ACTTCGTTCA AGTGGATCCA GGGCAGGTTG 
     1261 GCTTAATAAA TTCAAATGTG TCTATGGTAG ATTTAACCAG TGTTACCTTA GAAGATGTAA 
     1321 CACCTAAGAA GTCACCACCA CCAAAGACAA ATTTAACCCC TGGCCAAAGT GATTCAGCAA 
     1381 AAAGGGAAGT AAAGCAGAAG ATCAGTCCCT ACTTTAAAAG TAATGATGTG GTGTGCAAAA 
     1441 ATCAAGATGA GCTGAGAAAT CGTAGTGTGA AAGTCATTTG TTTGGGAAGC CTAGCATCTA 
     1501 AATTGTCCAG AAAATACGTA AAGGCTAAAA AATCAATAGA TAAGGATGAA GAATTTGCCG 
     1561 GTTCTAGTCC ACAGAGTTCC AAATACACAG TTGTTAAGAG CCTGATTGAT AACTCTTCAG 
     1621 AAATTGAGGA CGAGGATCAA ATTTTGGAGA ACAGTTCTCA AAAAGAAAAC GTGTTTAAAT 
     1681 GTGATTCTCT AAAGGAAGAG TGCATTCCTG AACATATGGT AAGAGAAAGT AAAATAATGG 
     1741 AAGCCGAAAG CCAAAAGGCT ACCCGGGAAT GTGAGAAATC AGCCCTCACC CCTGGATTCT 
     1801 CAGATAATGC GATCATGTTA TTCTCACCAG ATTTCACTCT TAGGAATACA TTAAAGTCTA 
     1861 CTTCAGAAGA CAGTCTTGTA AAGCAAGAGT GTATCAAAGA AGTGGTTGAA AAACGTGAGG 
     1921 CATGTCATTG TGAAGAAGTA AAAATGACTG TTGCTTCAGA AGCTAAAATA CAGCTGTCAG 
     1981 ATTCAGAGGC AAAATCTCAT AGTTCTGCAG ATGATGCTTC TGCATGGAGT AACATCCAAG 
     2041 AGGCTCCTCT GCAGGATGAC AGTTGCTTAA ACAATGATAT CCCTCACAGC ATTCCTTTGG 
     2101 AGCAGGGGTC AAGCTGCAAT GGTCCTGGTC AAACAACCGG TCATCCTTAC TACCTTCGGA 
     2161 GTTTCCTTGT GGTGCTGAAA ACCGTACTTG AGAATGAAGA TGATATGTTG CTCTTTGATG 
     2221 AGCAGGAGAA GGGAATTGTA ACTAAATTTT ATCAGTTATC AGCTACTGGT CAGAAGTTAT 
     2281 ATGTAAGGCT CTTTCAACGT AAATTAAGCT GGATTAAGAT GACCAAATTA GAGTATGAAG 
     2341 AGATTGCCTT AGACTTAACA CCTGTGATTG AAGAATTGAC GAATGCAGGC TTTCTACAGA 
     2401 CAGAATCTGA GTTGCAAGAA CTCTCTGAAG TGCTTGAACT CCTTTCTGCT CCTGAACTAA 
     2461 AATCCCTAGC CAAGACCTTC CACTTGGTGA ATCCCAATGG ACAGAAACAG CAGCTGGTGG 
     2521 ACGCCTTTCT CAAATTGGCC AAACAGCGTT CAGTCTGCAC TTGGGGCAAG AATAAGCCTG 
     2581 GAATTGGTGC AGTGATTTTA AAAAGAGCCA AAGCCTTGGC TGGACAGTCA GTACGAATCT 
     2641 GTAAAGGCCC CAGGGCTGTG TTTTCCCGCA TCTTGCTACT GTTTTCGTTG ACCGACTCAA 
     2701 TGGAAGATGA AGACGCCGCT TGTGGAGGTC AGGGACAGCT TTCAACAGTC CTGTTGGTCA 
     2761 ACCTCGGCCG AATGGAGTTT CCTAGTTACA CCATCAATCG GAAAACCCAC ATCTTCCAAG 
     2821 ACAGAGATGA TCTTATCAGA TATGCAGCAG CCACGCACAT GCTGAGTGAC ATTTCTTCCG 
     2881 CAATGGCCAA TGGGAACTGG GAAGAAGCTA AGGAGCTCGC TCAGTGTGCA AAAAGGGATT 
     2941 GGAACAGACT GAAAAACCAC CCTTCTCTGA GATGCCACGA AGATTTACCA CTCTTCCTGC 
     3001 GGTGTTTCAC TGTTGGGTGG ATTTATACAA GGATTTTGTC TCGGTTTGTG GAAATACTGC 
     3061 AGAGACTTCA CATGTATGAG GAAGCCGTCA GAGAACTTGA AAGCCTTTTG TCTCAGAGAA 
     3121 TTTATTGTCC TGACAGCAGA GGCCGATGGT GGGATCGACT GGCCCTTAAT TTACACCAGC 
     3181 ACTTGAAGCG CCTGGAACCG ACTATCAAGT GCATCACAGA GGGGCTGGCG GATCCGGAAG 
     3241 TCAGAACGGG ACACCGCCTT TCACTGTATC AGCGAGCCGT GCGCCTGCGA GAGTCTCCGA 
     3301 GCTGTAAAAA GTTCAAGCAC CTCTTCCAGC AGCTCCCAGA AATGGCTGTG CAAGATGTGA 
     3361 AACACGTGAC CATCACAGGC AGGCTGTGCC CACAGCGTGG GATGTGCAAG TCTGTGTTTG 
     3421 TGATGGAGGC CGGGGAGGCC GCTGACCCCA CCACGGTCCT GTGCTCTGTG GAGGAGCTGG 
     3481 CACTGGCCCA TTACAGACGC AGCGGTTTTG ACCAGGGGAT TCATGGCGAA GGGTCCACCT 
     3541 TCAGCACCCT GTATGGCCTC CTCCTGTGGG ACATCATCTT CATGGATGGG ATTCCGGATG 
     3601 TCTTCAGAAA CGCCTGTCAG GCATTCCCCC TGGACTTGTG CACAGACAGC TTCTTCACAA 
     3661 GCAGACGCCC AGCCCTTGAG GCCAGGCTGC AGCTGATTCA TGATGCCCCC GAGGAGAGCC 
     3721 TGCGGGCCTG GGTGGCAGCC ACGTGGCATG AGCAGGAAGG CAGAGTGGCT TCCCTTGTCA 
     3781 GCTGGGATCG CTTCACGTCT CTTCAGCAAG CTCAGGATCT TGTCTCCTGC CTGGGGGGCC 
     3841 CTGTGCTCAG TGGTGTGTGC AGGCACCTGG CTGCTGACTT TCGACACTGT CGAGGGGGCC 
     3901 TCCCCGACCT GGTGGTGTGG AACTCCCAGA GCCGTCACTT TAAGCTGGTG GAAGTTAAAG 
     3961 GCCCCAATGA TCGTCTTTCA CATAAGCAGA TGATCTGGCT GGCTGAACTG CAGAAGCTGG 
     4021 GGGCTGAAGT AGAAGTCTGC CACGTGGTTG CAGTTGGAGC TAAGAGCCAA AGCCTTAGCA 
     4081 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4141 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4201 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4261 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4321 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4381 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4441 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4501 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4561 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4621 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4681 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4741 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4801 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4861 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4921 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4981 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5041 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5101 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5161 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5221 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5281 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5341 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5401 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5461 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5521 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5581 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5641 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5701 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5761 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5821 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5881 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5941 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6001 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6061 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6121 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6181 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6241 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6301 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6361 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6421 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6481 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6541 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6601 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6661 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6721 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6781 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6841 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6901 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6961 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7021 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7081 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7141 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7201 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7261 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7321 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7381 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7441 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7501 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7561 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7621 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7681 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7741 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7801 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7861 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7921 AACGCGAATT TT
//