LOCUS       RC224139                8103 bp    DNA     circular  SYN 
DEFINITION  SRGAP2 (Myc-DDK-tagged)-Human SLIT-ROBO Rho GTPase activating protein 2 (SRGAP2), transcript variant 1
ACCESSION   NM_015326
VERSION     NM_015326.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4258..4349 
                     /label="Myc-DDK"  
     misc_feature    4379..4964 
                     /label="PolyA signal"  
     misc_feature    4436..4455 
                     /label="Restriction Enzyme"  
     misc_feature    5240..5912 
                     /label="ColE1"  
     misc_feature    6342..7136 
                     /label="Kan/Neo"  
     misc_feature    7171..7473 
                     /label="SV40 ori"  
     misc_feature    7559..8036 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4233..4240 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4233 
                     /label="SRGAP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGTCTCCA GCCAAATTCA AAAAGGATAA GGAGATCATA GCAGAGTACG 
     1081 ATACTCAGGT CAAAGAGATC CGTGCTCAGC TCACAGAGCA GATGAAATGC CTGGACCAGC 
     1141 AGTGTGAGCT TCGGGTGCAA CTGTTGCAGG ACCTCCAGGA CTTCTTCCGA AAGAAGGCAG 
     1201 AGATTGAGAT GGACTACTCC CGCAACCTGG AGAAGCTGGC AGAACGCTTC CTGGCCAAGA 
     1261 CACGCAGCAC CAAGGACCAG CAATTCAAGA AGGATCAGAA TGTTCTCTCT CCAGTCAACT 
     1321 GCTGGAATCT CCTCTTAAAC CAGGTGAAGC GGGAAAGCAG GGACCATACC ACCCTGAGTG 
     1381 ACATCTACCT GAATAATATC ATTCCTCGAT TTGTACAAGT CAGCGAGGAC TCAGGAAGAC 
     1441 TCTTTAAAAA GAGTAAAGAA GTCGGCCAGC AGCTCCAAGA TGATTTGATG AAGGTCCTGA 
     1501 ACGAGCTCTA CTCGGTGATG AAGACATATC ACATGTACAA TGCCGACAGC ATCAGTGCTC 
     1561 AGAGCAAACT AAAGGAGGCG GAGAAGCAGG AGGAGAAGCA AATTGGTAAA TCGGTAAAGC 
     1621 AGGAGGACCG GCAGACCCCA CGCTCCCCTG ACTCCACGGC CAACGTTCGC ATTGAGGAGA 
     1681 AACATGTCCG GAGGAGCTCA GTGAAGAAGA TTGAGAAGAT GAAGGAGAAG CGTCAAGCCA 
     1741 AGTACACGGA GAATAAGCTG AAGGCCATCA AAGCCCGGAA TGAGTACTTG CTGGCTTTGG 
     1801 AGGCAACCAA TGCATCTGTC TTCAAGTACT ACATCCATGA CCTATCTGAC CTTATTGATC 
     1861 AGTGTTGTGA CTTAGGCTAC CATGCAAGTC TGAACCGGGC TCTACGCACC TTCCTCTCTG 
     1921 CTGAGTTAAA CCTGGAACAG TCGAAGCATG AGGGTCTGGA TGCCATCGAG AATGCAGTAG 
     1981 AAAACCTGGA TGCCACCAGT GACAAGCAGC GCCTCATGGA GATGTACAAC AACGTCTTCT 
     2041 GCCCCCCTAT GAAGTTTGAG TTTCAGCCCC ACATGGGGGA TATGGCTTCC CAGCTCTGTG 
     2101 CCCAGCAGCC TGTCCAGAGT GAGCTGGTAC AGAGATGCCA ACAACTGCAG TCTCGCTTAT 
     2161 CCACTCTAAA GATTGAAAAC GAAGAGGTAA AGAAGACAAT GGAGGCCACC CTGCAAACCA 
     2221 TCCAGGACAT TGTGACTGTC GAGGACTTTG ATGTGTCTGA CTGCTTCCAG TACAGCAACT 
     2281 CCATGGAGTC CGTCAAGTCC ACGGTCTCTG AAACCTTCAT GAGCAAGCCC AGCATTGCTA 
     2341 AGAGGAGAGC CAACCAGCAA GAGACAGAGC AGTTTTATTT CACAAAAATG AAAGAGTACC 
     2401 TGGAGGGCAG GAACCTCATC ACCAAGTTAC AAGCCAAGCA TGACCTTCTG CAGAAAACCC 
     2461 TGGGAGAAAG TCAGCGGACA GATTGCAGTC TAGCCAGGCG CAGCTCAACT GTGAGGAAAC 
     2521 AGGACTCCAG CCAGGCAATT CCTCTGGTGG TGGAAAGCTG TATCCGGTTT ATCAGCAGAC 
     2581 ACGGACTACA GCATGAAGGA ATTTTCCGGG TGTCAGGATC CCAGGTGGAA GTGAATGACA 
     2641 TCAAAAATGC CTTTGAGAGA GGAGAGGACC CCCTGGCTGG GGACCAGAAC GACCATGACA 
     2701 TGGATTCCAT AGCTGGTGTC CTGAAGCTTT ACTTCCGGGG GCTGGAACAC CCTCTCTTCC 
     2761 CCAAGGACAT CTTTCATGAC CTGATGGCCT GCGTCACAAT GGACAACCTG CAGGAGAGAG 
     2821 CTCTGCACAT CCGGAAAGTC CTCCTAGTCC TGCCCAAAAC CACTCTGATT ATCATGAGAT 
     2881 ACCTCTTTGC CTTCCTCAAT CATTTATCAC AGTTCAGTGA AGAGAACATG ATGGACCCCT 
     2941 ACAACCTCGC CATCTGCTTC GGGCCCTCGC TAATGTCAGT GCCAGAGGGC CACGACCAGG 
     3001 TGTCCTGCCA AGCCCACGTG AATGAGCTGA TCAAAACCAT CATCATCCAG CATGAGAACA 
     3061 TCTTCCCAAG CCCCAGGGAG CTGGAGGGCC CTGTCTACAG CAGAGGAGGA AGCATGGAGG 
     3121 ATTACTGTGA TAGCCCTCAT GGAGAGACTA CCTCGGTTGA AGACTCAACC CAGGATGTGA 
     3181 CCGCAGAGCA CCACACGAGC GATGACGAAT GTGAGCCCAT CGAGGCCATT GCCAAGTTTG 
     3241 ACTACGTGGG CCGGACAGCC CGAGAGCTGT CCTTTAAGAA GGGAGCATCC CTGCTGCTTT 
     3301 ACCAGCGGGC TTCCGACGAC TGGTGGGAAG GCCGGCACAA TGGCATCGAC GGACTCATCC 
     3361 CCCATCAGTA CATCGTGGTC CAAGACACCG AGGACGGTGT CGTGGAGAGG TCCAGCCCCA 
     3421 AGTCTGAGAT TGAGGTCATT TCTGAGCCAC CTGAAGAAAA GGTGACAGCC AGAGCGGGGG 
     3481 CCAGCTGTCC CAGTGGGGGT CATGTAGCCG ATATTTATCT TGCAAACATC AACAAGCAAA 
     3541 GGAAGCGTCC AGAATCTGGG AGCATCCGGA AAACTTTTCG GAGTGACAGC CATGGGCTGA 
     3601 GCAGTTCCCT GACTGACTCC TCCTCCCCAG GGGTGGGGGC TAGCTGCCGC CCATCCTCCC 
     3661 AGCCCATCAT GAGCCAGAGC CTCCCCAAAG AAGGGCCAGA TAAGTGTTCC ATCAGTGGGC 
     3721 ACGGGAGCCT CAACTCCATC AGCCGCCACT CATCCCTGAA GAATCGGCTG GATAGTCCAC 
     3781 AGATCCGGAA GACTGCCACA GCGGGAAGGT CAAAAAGCTT CAATAACCAT CGGCCCATGG 
     3841 ACCCTGAGGT CATTGCTCAG GATATTGAGG CAACAATGAA CTCGGCCCTG AATGAGCTAC 
     3901 GGGAACTAGA ACGGCAGAGC AGTGTCAAAC ACACCCCTGA CGTGGTTCTG GACACCTTGG 
     3961 AGCCCCTCAA AACCTCCCCA GTGGTGGCCC CCACGTCAGA GCCCTCCAGC CCTCTGCACA 
     4021 CCCAGCTCCT CAAGGACCCC GAGCCCGCCT TCCAGCGCAG CGCCAGTACT GCTGGGGACA 
     4081 TCGCCTGCGC CTTCCGGCCT GTCAAGTCTG TCAAGATGGC TGCCCCGGTC AAACCACCAG 
     4141 CCACACGGCC CAAGCCCACT GTCTTCCCCA AAACAAATGC CACTAGCCCT GGTGTCAACT 
     4201 CATCAACTTC CCCACAGTCT ACTGACAAGT CTTGTACTGT CAGCGGACCG ACGCGTACGC 
     4261 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4321 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4381 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4441 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4501 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4561 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4621 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4681 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4741 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4801 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4861 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4921 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4981 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5041 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5101 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5161 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5221 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5281 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5341 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5401 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5461 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5521 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5581 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5641 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5701 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5761 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5821 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5881 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5941 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6001 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6061 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6121 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6181 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6241 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6301 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6361 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6421 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6481 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6541 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6601 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6661 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6721 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6781 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6841 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6901 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6961 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7021 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7081 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7141 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7201 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7261 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7321 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7381 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7441 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7501 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7561 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7621 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7681 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7741 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7801 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7861 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7921 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7981 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8041 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8101 TTT
//