LOCUS       RC224104                7836 bp    DNA     circular  SYN 
DEFINITION  RIN3 (Myc-DDK-tagged)-Human Ras and Rab interactor 3 (RIN3)
ACCESSION   NM_024832
VERSION     NM_024832.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3993..4084 
                     /label="Myc-DDK"  
     misc_feature    4114..4699 
                     /label="PolyA signal"  
     misc_feature    4171..4190 
                     /label="Restriction Enzyme"  
     misc_feature    4975..5647 
                     /label="ColE1"  
     misc_feature    6077..6871 
                     /label="Kan/Neo"  
     misc_feature    6906..7208 
                     /label="SV40 ori"  
     misc_feature    7294..7771 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3975..3981 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3975 
                     /label="RIN3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCCGACAC GCCGGGGCGC CCGCGCGCGG GGACCCCACG GGTCCGGTTC 
     1081 CAGTTGTTGG CAAAGGAGAG GAAGAGGAAG AGGAAGATGG CATGCGGCTT TGTCTGCCAG 
     1141 CCAACCCGAA AAACTGCCTT CCTCACCGCC GGGGCATCAG CATCCTGGAG AAGCTCATCA 
     1201 AAACATGCCC GGTGTGGCTG CAGCTGAGTC TGGGCCAGGC AGAGGTGGCC AGGATCCTGC 
     1261 ACCGGGTGGT GGCTGGGATG TTCCTGGTTC GCCGGGACAG CAGCTCGAAG CAGCTGGTGC 
     1321 TCTGTGTCCA CTTTCCTTCT CTGAACGAAA GCTCGGCCGA GGTGCTCGAA TACACCATTA 
     1381 AGGAAGAAAA GTCGATATTG TACCTGGAAG GCTCGGCTCT TGTGTTTGAG GACATCTTCA 
     1441 GATTGATTGC GTTCTACTGT GTCAGTAGAG ACTTACTGCC CTTCACACTG CGGCTACCCC 
     1501 AGGCCATCCT TGAGGCCAGC AGCTTCACGG ACCTTGAGAC CATCGCCAAC CTGGGTCTGG 
     1561 GTTTCTGGGA CTCCTCGCTG AATCCTCCAC AAGAAAGAGG GAAGCCAGCA GAGCCCCCAA 
     1621 GAGACCGGGC CCCCGGATTC CCCCTAGTCT CCAGCCTCAG GCCCACAGCC CATGACGCAA 
     1681 ACTGTGCCTG TGAAATCGAG CTGTCGGTAG GAAATGACCG CCTGTGGTTT GTGAATCCTA 
     1741 TTTTCATCGA GGACTGCAGC AGCGCCCTGC CCACCGACCA GCCACCTCTT GGAAATTGCC 
     1801 CTGCACGCCC TTTGCCGCCC ACCTCTGATG CCACCTCACC CACCTCCAGG TGGGCCCCAC 
     1861 GCCGCCCACC ACCCCCTCCC CCAGTGCTGC CCCTGCAGCC CTGCAGCCCA GCCCAGCCCC 
     1921 CTGTGCTCCC TGCTCTTGCC CCCGCCCCTG CCTGTCCTTT GCCCACCTCT CCCCCAGTGC 
     1981 CTGCCCCCCA CGTCACACCC CATGCCCCAG GTCCCCCAGA CCATCCGAAC CAGCCGCCCA 
     2041 TGATGACCTG CGAGAGACTC CCATGCCCCA CTGCAGGCCT GGGCCCCCTC AGGGAGGAAG 
     2101 CGATGAAGCC AGGGGCAGCC TCCAGTCCCT TGCAGCAGGT CCCCGCCCCG CCACTGCCTG 
     2161 CGAAGAAGAA CCTTCCCACT GCCCCTCCCA GACGCCGCGT TTCCGAGAGG GTGTCCTTAG 
     2221 AAGACCAAAG TCCGGGGATG GCGGCAGAGG GGGACCAGCT CAGCCTGCCT CCCCAAGGGA 
     2281 CCTCAGACGG CCCTGAGGAC ACGCCCCGGG AGAGCACGGA GCAAGGCCAG GACACAGAGG 
     2341 TGAAAGCCAG CGATCCTCAC AGCATGCCAG AGCTGCCCAG GACAGCCAAA CAACCCCCAG 
     2401 TCCCGCCCCC CAGGAAAAAA CGGATCTCTC GACAACTGGC CTCGACCCTC CCAGCTCCCT 
     2461 TAGAGAACGC TGAGCTCTGC ACACAGGCGA TGGCCTTGGA GACACCCACG CCGGGTCCAC 
     2521 CCAGAGAGGG CCAAAGCCCT GCTTCTCAGG CTGGGACTCA GCACCCTCCT GCCCAGGCCA 
     2581 CTGCCCATTC CCAGAGCTCT CCAGAGTTCA AGGGCTCCCT GGCCTCCCTC TCAGACAGCT 
     2641 TGGGGGTGTC TGTCATGGCC ACCGACCAGG ACTCCTACTC CACCAGCAGC ACGGAGGAGG 
     2701 AGCTGGAGCA GTTCAGCAGC CCCAGCGTGA AGAAGAAGCC CTCCATGATC CTGGGCAAGG 
     2761 CTCGGCACCG GCTGAGCTTT GCCAGTTTCA GCAGCATGTT CCACGCTTTC CTCTCCAACA 
     2821 ACCGCAAGCT GTACAAGAAG GTGGTGGAGC TGGCGCAGGA CAAGGGCTCG TACTTTGGCA 
     2881 GCCTGGTGCA GGACTACAAG GTGTACAGCC TGGAGATGAT GGCGCGCCAG ACCTCCAGCA 
     2941 CGGAGATGCT GCAGGAGATT CGCACCATGA TGACCCAGCT CAAGAGCTAC CTGCTGCAGA 
     3001 GCACCGAGCT CAAGGCCCTG GTGGACCCCG CCCTGCACTC CGAGGAGGAG CTCGAAGCAA 
     3061 TTGTAGAGTC TGCCTTGTAC AAATGTGTCC TGAAGCCCCT GAAGGAAGCC ATCAACTCAT 
     3121 GCCTGCATCA GATCCACAGC AAGGATGGTT CGCTGCAGCA GCTCAAGGAG AACCAGTTAG 
     3181 TGATCCTGGC CACCACCACC ACTGACCTAG GTGTGACCAC CAGCGTGCCG GAGGTGCCCA 
     3241 TGATGGAGAA GATCCTGCAG AAGTTCACCA GCATGCACAA GGCCTACTCA CCTGAGAAGA 
     3301 AGATCTCCAT CCTGCTCAAG ACCTGCAAAC TCATCTACGA CTCCATGGCC CTCGGCAACC 
     3361 CAGGGAAGCC CTATGGGGCG GATGACTTCC TGCCTGTGCT CATGTATGTG CTGGCCCGCA 
     3421 GCAACCTCAC GGAGATGCTT CTCAATGTGG AGTACATGAT GGAGCTCATG GACCCCGCCC 
     3481 TGCAGCTGGG GGAGGGTTCC TACTATCTGA CCACCACCTA CGGGGCCCTG GAGCACATCA 
     3541 AGAGCTACGA CAAGATCACG GTGACCCGGC AGCTGAGTGT GGAGGTGCAG GACTCCATCC 
     3601 ACCGCTGGGA GCGCCGGCGT ACTCTCAACA AGGCCCGGGC CTCCCGCTCC TCCGTACAGG 
     3661 ACTTCATCTG CGTGTCGTAC CTGGAGCCCG AGCAGCAGGC GCGGACGCTG GCGTCGCGGG 
     3721 CGGACACCCA GGCCCAGGCG CTGTGCGCGC AGTGCGCGGA GAAGTTCGCG GTGGAGCGGC 
     3781 CGCAGGCGCA CCGGCTGTTC GTGCTGGTGG ACGGGCGCTG CTTCCAGCTG GCGGACGACG 
     3841 CGCTGCCGCA CTGCATCAAG GGCTACCTGC TGCGCAGCGA GCCCAAGCGC GACTTCCACT 
     3901 TTGTCTACCG GCCCCTGGAC GGTGGTGGCG GCGGCGGCGG CGGGAGCCCG CCCTGCCTGG 
     3961 TGGTGCGGGA GCCCAACTTC CTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4021 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4081 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4141 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4201 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4261 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4321 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4381 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4441 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4501 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4561 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4621 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4681 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4741 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4801 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4861 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4921 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4981 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5041 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5101 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5161 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5221 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5281 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5341 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5401 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5461 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5521 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5581 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5641 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5701 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5761 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5821 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5881 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5941 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6001 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6061 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6121 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6181 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6241 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6301 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6361 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6421 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6481 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6541 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6601 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6661 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6721 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6781 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6841 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6901 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6961 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7021 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7081 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7141 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7201 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7261 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7321 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7381 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7441 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7501 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7561 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7621 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7681 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7741 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7801 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//