LOCUS       RC224083                9528 bp    DNA     circular  SYN 
DEFINITION  NFAT5 (Myc-DDK-tagged)-Human nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant 2
ACCESSION   NM_138713
VERSION     NM_138713.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5685..5776 
                     /label="Myc-DDK"  
     misc_feature    5806..6391 
                     /label="PolyA signal"  
     misc_feature    5863..5882 
                     /label="Restriction Enzyme"  
     misc_feature    6667..7339 
                     /label="ColE1"  
     misc_feature    7769..8563 
                     /label="Kan/Neo"  
     misc_feature    8598..8900 
                     /label="SV40 ori"  
     misc_feature    8986..9463 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5667..5673 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5667 
                     /label="NFAT5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCTCGGAC TTCATCTCAT TGCTCAGCGC GGACCTAGAC CTGGAATCGC 
     1081 CCAAGTCCCT CTACTCGCGA GATTCTCTGA AGTTACACCC ATCACAGAAT TTTCATAGAG 
     1141 CTGGACTATT GGAAGAATCT GTCTATGATC TTCTCCCAAA GGAGTTACAG TTACCTCCAT 
     1201 CTAGAGAAAC ATCTGTAGCA TCAATGAGTC AGACAAGCGG TGGTGAGGCA GGCTCGCCTC 
     1261 CTCCAGCTGT TGTTGCTGCT GATGCTTCTT CAGCTCCCTC CTCTTCCTCC ATGGGCGGTG 
     1321 CTTGCAGCTC CTTTACCACC TCTTCCAGCC CTACCATTTA TTCTACCTCA GTCACCGACA 
     1381 GCAAGGCTAT GCAAGTGGAG AGCTGCTCCT CAGCCGTGGG GGTAAGTAAC AGAGGGGTAA 
     1441 GTGAAAAGCA GTTAACCAGT AACACAGTTC AGCAGCATCC ATCAACACCG AAGAGGCACA 
     1501 CAGTCTTGTA CATCTCACCA CCACCTGAGG ACTTGCTGGA TAACAGTCGG ATGTCCTGCC 
     1561 AGGATGAGGG GTGTGGATTG GAATCTGAGC AGAGCTGCAG TATGTGGATG GAGGATTCCC 
     1621 CCTCCAACTT CAGTAACATG AGCACCAGTT CCTACAATGA TAACACTGAG GTACCTCGTA 
     1681 AATCACGAAA ACGAAATCCA AAGCAGAGGC CGGGGGTCAA ACGACGAGAT TGTGAAGAAT 
     1741 CTAATATGGA TATATTTGAT GCCGACAGTG CCAAAGCACC TCACTATGTG CTTTCTCAGC 
     1801 TTACCACGGA CAACAAAGGC AACTCAAAAG CGGGAAATGG AACATTGGAA AACCAAAAAG 
     1861 GAACTGGAGT AAAGAAGAGC CCTATGTTGT GTGGACAATA TCCTGTTAAA AGTGAGGGAA 
     1921 AGGAGCTGAA GATAGTTGTA CAACCTGAGA CACAGCACCG AGCTCGGTAC CTGACTGAGG 
     1981 GCAGCCGTGG CTCAGTGAAA GATAGAACAC AGCAAGGCTT TCCTACAGTA AAGCTGGAAG 
     2041 GCCATAATGA ACCTGTAGTG TTGCAAGTGT TTGTGGGCAA CGACTCTGGA CGAGTGAAAC 
     2101 CACATGGATT TTATCAGGCC TGCAGAGTAA CTGGACGAAA TACAACTCCT TGCAAAGAAG 
     2161 TGGACATTGA AGGCACTACT GTTATAGAAG TCGGCCTTGA TCCTAGCAAC AACATGACAC 
     2221 TGGCGGTGGA CTGCGTAGGG ATATTGAAAT TGAGGAATGC TGATGTCGAA GCCAGAATAG 
     2281 GAATTGCTGG TTCCAAGAAG AAAAGCACTC GTGCCAGATT GGTTTTTCGA GTTAATATCA 
     2341 TGAGGAAAGA TGGCTCCACT TTGACACTGC AAACACCCTC TTCTCCAATT TTGTGTACTC 
     2401 AGCCAGCAGG AGTGCCAGAA ATCTTAAAGA AAAGCTTGCA TAGCTGTTCA GTGAAAGGAG 
     2461 AAGAAGAAGT GTTTTTAATC GGCAAGAACT TTCTGAAAGG AACTAAAGTT ATTTTCCAAG 
     2521 AAAATGTTTC TGATGAAAAC TCTTGGAAGT CAGAAGCTGA AATTGATATG GAACTATTTC 
     2581 ATCAGAATCA TCTTATTGTG AAGGTTCCTC CCTATCATGA CCAACATATA ACTTTGCCTG 
     2641 TGTCAGTGGG AATATATGTA GTGACAAATG CTGGAAGATC TCATGATGTT CAACCATTCA 
     2701 CTTACACTCC AGACCCAGCA GCAGCTGGTG CTTTGAATGT AAATGTGAAG AAGGAAATAT 
     2761 CTAGTCCAGC AAGACCTTGC TCTTTTGAAG AGGCCATGAA AGCAATGAAA ACTACTGGAT 
     2821 GTAATTTAGA TAAGGTAAAT ATTATCCCTA ATGCCCTGAT GACTCCACTC ATACCAAGCA 
     2881 GTATGATTAA GAGTGAAGAT GTTACTCCAA TGGAAGTAAC AGCAGAAAAA AGATCTTCCA 
     2941 CTATTTTTAA GACTACAAAG TCTGTTGGAT CAACTCAGCA AACATTAGAA AACATCTCAA 
     3001 ACATAGCAGG AAATGGCTCT TTTTCATCAC CATCATCTTC CCACCTACCT TCTGAAAATG 
     3061 AAAAACAGCA GCAGATTCAG CCCAAGGCAT ACAACCCAGA GACCCTGACA ACTATTCAAA 
     3121 CCCAGGACAT CTCACAGCCT GGTACTTTTC CAGCAGTTTC TGCTTCTAGT CAGCTGCCCA 
     3181 ACAGCGATGC ACTATTGCAG CAGGCTACAC AGTTTCAGAC AAGAGAAACT CAGTCTAGAG 
     3241 AGATATTACA GTCAGATGGT ACAGTGGTTA ATTTGTCACA ACTGACTGAG GCATCACAAC 
     3301 AACAGCAGCA GTCACCACTA CAAGAACAAG CACAGACTTT ACAGCAGCAG ATTTCATCAA 
     3361 ATATTTTTCC ATCACCAAAT AGTGTGAGTC AGCTTCAGAA TACTATTCAG CAGCTGCAAG 
     3421 CAGGGAGTTT CACAGGCAGT ACTGCTAGTG GCAGCAGTGG AAGTGTTGAC TTGGTCCAAC 
     3481 AAGTTTTAGA GGCACAGCAG CAGTTATCTT CAGTTTTATT TTCTGCTCCA GATGGTAATG 
     3541 AGAATGTTCA AGAGCAGCTT AGTGCAGATA TTTTTCAACA AGTCAGTCAA ATTCAGAGTG 
     3601 GTGTAAGCCC TGGAATGTTT TCCTCAACAG AGCCAACAGT CCATACCAGA CCAGATAATT 
     3661 TATTACCTGG AAGAGCTGAA AGTGTTCATC CACAGTCTGA AAACACGTTA TCTAATCAAC 
     3721 AGCAGCAGCA GCAGCAGCAA CAGCAAGTGA TGGAATCTTC AGCCGCAATG GTGATGGAGA 
     3781 TGCAACAGAG TATCTGCCAG GCAGCTGCCC AGATTCAGTC AGAGTTATTC CCTTCAACTG 
     3841 CTTCAGCAAA TGGAAACCTT CAGCAATCGC CAGTTTACCA GCAGACTTCT CACATGATGA 
     3901 GTGCATTGTC TACCAATGAG GATATGCAAA TGCAGTGTGA ATTGTTTTCT TCTCCTCCTG 
     3961 CAGTTTCTGG AAATGAAACT TCTACAACTA CCACACAGCA GGTTGCAACC CCTGGCACTA 
     4021 CCATGTTTCA GACATCAAGT TCAGGAGATG GAGAAGAAAC TGGAACACAA GCAAAACAGA 
     4081 TTCAGAACAG TGTCTTTCAG ACCATGGTCC AAATGCAACA TAGTGGGGAC AATCAACCTC 
     4141 AAGTTAACCT TTTTTCATCC ACAAAAAGTA TGATGAGTGT TCAGAATAGT GGTACCCAAC 
     4201 AACAAGGTAA TGGTTTATTC CAGCAAGGGA ATGAGATGAT GTCACTTCAA TCTGGAAATT 
     4261 TTTTGCAGCA GTCTTCTCAT TCACAGGCCC AACTTTTTCA TCCTCAAAAT CCTATTGCCG 
     4321 ATGCTCAGAA CCTTTCCCAG GAAACTCAAG GTTCTCTCTT TCATAGTCCA AATCCTATTG 
     4381 TCCACAGTCA GACTTCTACA ACCTCCTCTG AACAAATGCA GCCTCCAATG TTTCACTCTC 
     4441 AAAGTACCAT TGCTGTGTTA CAGGGCTCTT CAGTTCCTCA AGACCAGCAG TCAACCAACA 
     4501 TATTTCTTTC CCAGAGTCCC ATGAATAATC TTCAGACTAA CACAGTAGCC CAAGAAGCAT 
     4561 TTTTTGCAGC ACCGAACTCA ATTTCTCCAC TTCAGTCAAC ATCAAACAGT GAACAACAAG 
     4621 CTGCTTTCCA ACAGCAAGCT CCAATATCAC ACATCCAGAC TCCTATGCTT TCCCAAGAAC 
     4681 AGGCACAACC CCCGCAGCAG GGTTTATTTC AGCCTCAGGT GGCCCTGGGC TCCCTTCCAC 
     4741 CTAATCCAAT GCCTCAAAGC CAACAAGGAA CCATGTTCCA GTCACAGCAC TCAATAGTTG 
     4801 CCATGCAGAG TAACTCTCCA TCCCAGGAAC AGCAGCAGCA GCAGCAACAG CAGCAGCAAC 
     4861 AGCAGCAGCA ACAACAACAG AGCATTTTAT TCAGTAATCA GAATACCATG GCTACAATGG 
     4921 CGTCTCCAAA GCAACCACCA CCAAACATGA TATTCAACCC AAATCAAAAT CCAATGGCTA 
     4981 ATCAGGAGCA ACAGAACCAG TCAATTTTTC ACCAACAAAG TAACATGGCC CCAATGAATC 
     5041 AAGAGCAACA GCCCATGCAA TTTCAGAGTC AGTCCACAGT TTCCTCACTT CAGAACCCAG 
     5101 GTCCTACCCA GTCGGAATCA TCACAGACCC CCTTGTTCCA TAGCTCTCCT CAGATTCAGT 
     5161 TGGTACAAGG GTCACCTAGT TCTCAAGAGC AGCAAGTAAC TCTCTTCTTA TCTCCAGCAT 
     5221 CCATGTCTGC CTTGCAGACC AGTATAAATC AACAAGATAT GCAACAGTCT CCTCTTTATT 
     5281 CCCCTCAGAA CAACATGCCT GGAATTCAAG GAGCCACATC TTCGCCTCAA CCACAGGCTA 
     5341 CTTTATTTCA CAACACAGCA GGAGGCACAA TGAACCAACT GCAGAATTCT CCTGGCTCAT 
     5401 CTCAGCAGAC ATCAGGAATG TTCTTATTTG GCATTCAAAA TAACTGTAGT CAGCTTTTAA 
     5461 CCTCTGGACC AGCTACATTG CCTGATCAGT TGATGGCCAT AAGTCAGCCA GGCCAACCAC 
     5521 AAAACGAGGG CCAGCCACCT GTGACAACAC TTCTTTCTCA GCAAATGCCA GAGAATTCTC 
     5581 CACTGGCATC CTCTATAAAC ACCAACCAGA ACATCGAAAA GATTGATTTG CTTGTTTCAT 
     5641 TGCAAAACCA AGGGAACAAC TTGACTGGCT CCTTTACGCG TACGCGGCCG CTCGAGCAGA 
     5701 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5761 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5821 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5881 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5941 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     6001 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     6061 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     6121 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     6181 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     6241 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     6301 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     6361 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     6421 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     6481 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6541 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6601 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6661 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6721 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6781 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6841 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6901 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6961 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     7021 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     7081 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     7141 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     7201 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     7261 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     7321 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     7381 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     7441 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     7501 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7561 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7621 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7681 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7741 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7801 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7861 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7921 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7981 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     8041 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     8101 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     8161 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     8221 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     8281 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     8341 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     8401 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     8461 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     8521 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8581 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8641 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8701 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8761 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8821 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8881 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8941 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     9001 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     9061 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     9121 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     9181 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     9241 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     9301 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     9361 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     9421 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     9481 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//