LOCUS       RC224081                8037 bp    DNA     circular  SYN 
DEFINITION  MFHAS1 (Myc-DDK-tagged)-Human malignant fibrous histiocytoma amplified sequence 1 (MFHAS1)
ACCESSION   NM_004225
VERSION     NM_004225.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4194..4285 
                     /label="Myc-DDK"  
     misc_feature    4315..4900 
                     /label="PolyA signal"  
     misc_feature    4372..4391 
                     /label="Restriction Enzyme"  
     misc_feature    5176..5848 
                     /label="ColE1"  
     misc_feature    6278..7072 
                     /label="Kan/Neo"  
     misc_feature    7107..7409 
                     /label="SV40 ori"  
     misc_feature    7495..7972 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4176..4182 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4176 
                     /label="MFHAS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGGATG GACAGTGGCA ACCTGAAGAC CGCGAGGCTG TGGCGGGACG 
     1081 CCGCCCTGCG TGCCAGGAAG CTGCGGAGCA ACCTGCGCCA GCTCACGCTT ACCGCCGCCG 
     1141 GGGCCTGCCC CGGGGCCGGG GCCGACGCGC TCGAGTCCCC CGCCTCCCCC CAGCTCGTGC 
     1201 TGCCGGCCAA CCTCGGGGAC ATTGAGGCAC TGAACCTGGG GAACAACGGC CTGGAGGAGG 
     1261 TACCCGAGGG GCTGGGGTCG GCGCTGGGCA GCCTGCGCGT CCTGGTCCTG CGCAGGAACC 
     1321 GCTTCGCCCG GCTGCCCCCG GCGGTGGCCG AGCTCGGCCA CCACCTCACC GAGCTGGACG 
     1381 TGAGCCACAA CCGGCTGACC GCCCTGGGCG CGGAGGTGGT GAGTGCTCTG AGGGAGCTGC 
     1441 GGAAGCTCAA CCTCAGCCAC AACCAGCTGC CCGCCCTGCC CGCCCAGCTG GGCGCTCTCG 
     1501 CTCACCTGGA GGAGCTGGAT GTCAGCTTTA ACCGGCTGGC GCACCTGCCT GACTCCCTCT 
     1561 CCTGCCTCTC CCGCCTGCGC ACCCTGGACG TGGATCACAA CCAGCTCACT GCCTTCCCCC 
     1621 GGCAGCTGCT GCAGCTGGTG GCCCTGGAGG AGCTGGACGT GTCCAGCAAC CGGCTGCGGG 
     1681 GCCTGCCTGA GGATATCAGT GCCCTGCGTG CCCTCAAGAT CCTCTGGCTG AGTGGGGCCG 
     1741 AGCTTGGCAC GCTGCCCGCC GGCTTCTGCG AGCTGGCCAG TTTGGAGAGC CTCATGCTAG 
     1801 ACAACAACGG GCTGCAGGCT CTGCCCGCCC AGTTCAGCTG CCTGCAGCGG CTCAAAATGC 
     1861 TCAACCTCTC CTCCAACCTC TTCGAGGAGT TCCCTGCCGC GCTGCTGCCC CTGGCTGGTC 
     1921 TGGAGGAGCT CTACCTTAGT CGCAACCAGC TCACCTCGGT GCCATCCCTT ATCTCGGGCC 
     1981 TGGGCCGGCT TCTCACCTTG TGGCTGGATA ATAACCGCAT CCGCTACCTG CCGGACTCCA 
     2041 TCGTGGAGCT GACCGGCCTG GAGGAGCTCG TGCTGCAGGG GAACCAGATC GCGGTGCTGC 
     2101 CCGACCACTT TGGCCAGCTC TCCCGGGTGG GTTTGTGGAA GATCAAAGAC AACCCACTGA 
     2161 TCCAGCCCCC CTACGAGGTC TGCATGAAGG GGATCCCCTA CATCGCAGCC TACCAGAAGG 
     2221 AACTGGCTCA TTCCCAGCCG GCGGTGCAGC CCCGGCTCAA GCTGCTCCTG ATGGGGCATA 
     2281 AGGCTGCAGG AAAGACTTTG CTGCGCCACT GCCTCACCGA GGAGAGAGTG GAGGGATGCC 
     2341 CAGGAGGAGG GGACAAGGAG AAGTGCTACC CACCGTCACC TCCCCCTGTG AGCAAGGGCA 
     2401 TCGAGGTGAC CAGCTGGACG GCCGATGCCT CCCGGGGCCT GCGGTTCATC GTGTATGACT 
     2461 TAGCTGGGGA TGAAAGTTAT GAGGTGATCC AGCCCTTCTT CCTGTCCCCA GGGGCCCTAT 
     2521 ACGTGCTGGT GGTCAACTTG GCCACCTATG AGCCTCGCCA CTTTCCTACC ACCGTGGGCT 
     2581 CCTTCTTGCA TCGGGTCGGG GCGAGAGTGC CCCACGCGGT GGTGTGCATC GTGGGCACCC 
     2641 ACGCAGACCT GTGCGGAGAG CGTGAGCTGG AGGAGAAATG TCTGGACATT CACCGCCAGA 
     2701 TCGCCCTGCA GGAGAAGCAC GACGCGGAGG GACTGAGCCG CTTGGCCAAG GTGGTGGACG 
     2761 AGGCACTGGC CCGGGACTTC GAGCTGCGCT CTGCCAGCCC CCACGCAGCC TACTATGGCG 
     2821 TTTCGGACAA GAACCTTCGA CGGCGCAAGG CCCATTTTCA ATACCTGCTC AACCACCGGC 
     2881 TGCAGATCCT CTCCCCCGTG TTGCCTGTTA GCTGCAGGGA CCCGCGCCAC TTACGACGCC 
     2941 TTCGGGACAA GTTGCTGTCA GTTGCTGAGC ACCGAGAGAT CTTCCCCAAC TTACACAGAG 
     3001 TACTGCCTCG ATCCTGGCAG GTGCTGGAGG AACTGCATTT CCAGCCACCT CAGGCCCAGC 
     3061 GACTGTGGCT AAGCTGGTGG GACTCGGCGC GCTTGGGCCT GCAGGCGGGT CTGACCGAGG 
     3121 ACCGACTGCA GAGTGCCCTC TCCTACCTGC ATGAGAGCGG CAAGCTACTC TACTTTGAGG 
     3181 ACAGTCCGGC TCTCAAGGAG CACGTCTTCC ACAACCTCAC CCGCCTCATC GACATCCTCA 
     3241 ATGTCTTCTT CCAGAGGGAT CCCTCTTTGC TGCTGCATAA GCTGCTCCTA GGGACCAGTG 
     3301 GAGAGGGCAA GGCGGAGGGG GAAAGCTCCC CGCCCATGGC GCGGTCCACC CCCAGCCAGG 
     3361 AACTGCTCCG GGCCACCCAG CTCCATCAGT ATGTGGAGGG CTTTCTGTTG CATGGGCTCT 
     3421 TGCCAGCTCA TGTCATTCGG TTGCTGCTTA AGCCTCATGT CCAGGCCCAG CAGGACTTGC 
     3481 AGCTGTTGCT GGAGCTGCTG GAGAAGATGG GACTCTGTTA CTGCCTCAAT AAACCCAAGG 
     3541 GCAAGCCTTT GAATGGGTCC ACAGCTTGGT ACAAGTTCCC ATGCTATGTG CAGAACGAGG 
     3601 TGCCCCATGC AGAAGCCTGG ATTAATGGGA CCAACCTAGC TGGGCAGTCT TTTGTGGCTG 
     3661 AGCAGTTGCA GATTGAATAT AGCTTTCCTT TTACTTTTCC ACTTGGGTTG TTTGCACGCT 
     3721 ACAGTGTCCA GATCAACAGC CATGTGGTGC ACAGGTCGGA TGGTAAATTT CAGATCTTTG 
     3781 CCTATAGAGG GAAAGTTCCT GTGGTTGTGA GTTACAGACC TGCCAGGGGA GTCCTGCAGC 
     3841 CAGACACCCT GTCCATTGCT AGCCATGCAT CATTACCAAA TATATGGACC GCATGGCAAG 
     3901 CCATAACCCC CTTGGTGGAG GAACTGAATG TCCTACTTCA GGAATGGCCT GGACTGCACT 
     3961 ACACCGTGCA CATTCTCTGT TCTAAGTGCC TTAAGAGAGG ATCGCCCAAT CCACATGCTT 
     4021 TTCCAGGGGA GTTGCTGAGT CAGCCCAGAC CGGAAGGAGT GGCAGAGATC ATTTGCCCCA 
     4081 AGAACGGCAG CGAGCGAGTA AATGTTGCCT TGGTTTACCC ACCCACGCCG ACTGTGATCA 
     4141 GCCCCTGTTC CAAGAAGAAT GTTGGTGAAA AGCACAGAAA CCAGACGCGT ACGCGGCCGC 
     4201 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4261 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4321 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4381 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4441 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4501 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4561 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4621 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4681 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4741 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4801 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4861 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4921 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4981 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5041 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5101 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5161 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5221 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5281 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5341 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5401 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5461 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5521 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5581 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5641 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5701 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5761 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5821 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5881 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5941 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6001 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6061 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6121 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6181 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6241 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6301 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6361 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6421 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6481 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6541 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6601 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6661 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6721 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6781 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6841 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6901 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6961 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7021 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7081 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7141 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7201 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7261 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7321 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7381 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7441 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7501 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7561 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7621 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7681 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7741 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7801 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7861 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7921 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7981 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
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