LOCUS       RC223963                8394 bp    DNA     circular  SYN 
DEFINITION  ZBED4 (Myc-DDK-tagged)-Human zinc finger, BED-type containing 4 (ZBED4)
ACCESSION   NM_014838
VERSION     NM_014838.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4551..4642 
                     /label="Myc-DDK"  
     misc_feature    4672..5257 
                     /label="PolyA signal"  
     misc_feature    4729..4748 
                     /label="Restriction Enzyme"  
     misc_feature    5533..6205 
                     /label="ColE1"  
     misc_feature    6635..7429 
                     /label="Kan/Neo"  
     misc_feature    7464..7766 
                     /label="SV40 ori"  
     misc_feature    7852..8329 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4533..4539 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4533 
                     /label="ZBED4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAATAAC TTGAAAACTT GTCCCAAAGA GGACGGTGAT TTCGTTTCTG 
     1081 ATAAAATAAA GTTTAAAATA GAAGAGGAAG ATGATGATGG AATTCCTCCT GATAGTTTGG 
     1141 AAAGAATGGA CTTTAAAAGC GAGCAGGAGG ACATGAAGCA GACAGACAGC GGTGGGGAGC 
     1201 GAGCGGGCCT CGGTGGGACG GGTTGCAGCT GCAAGCCCCC GGGGAAGTAC TTGTCTGCAG 
     1261 AGAGTGAGGA TGACTATGGC GCGCTGTTCT CCCAGTACAG CAGCACCCTA TACGACGTGG 
     1321 CCATGGAGGC CGTGACCCAA AGCCTCCTTT CCAGCCGGAA CATGAGCTCC AGGAAGAAGT 
     1381 CTCCAGCCTG GAAGCATTTT TTTATCTCTC CCCGAGACAG CACTAAAGCA ATATGCATGT 
     1441 ACTGTGTGAA GGAGTTCAGC AGAGGCAAAA ACGAGAAAGA CTTGAGTACC AGTTGTCTCA 
     1501 TGAGGCACGT GAGGCGCGCA CACCCGACCG TGCTCATTCA GGAAAATGGC AGCGTGTCTG 
     1561 CCGTGTCCTC GTTCCCCTCT CCCTCACTCC TGCTTCCACC GCAGCCTGCG GACGCGGGTG 
     1621 ACCTCAGCAC CATCCTCTCA CCCATCAAAC TTGTCCAGAA AGTGGCGTCT AAGATCCCGT 
     1681 CCCCCGATCG AATAACAGAG GAGTCTGTGT CTGTAGTTTC TTCTGAAGAA ATCTCCTCTG 
     1741 ACATGTCCGT TTCGGAGAAG TGCGGCAGAG AAGAAGCCCT GGTGGGGTCG TCTCCCCACC 
     1801 TCCCTGCTCT CCATTACGAT GAACCTGCAG AGAACTTAGC GGAGAAGAGC CTTCCACTTC 
     1861 CAAAGAGCAC CTCTGGGTCC AGGAGAAGGT CCGCTGTCTG GAAGCACTTC TACCTGTCGC 
     1921 CACTGGACAA CTCCAAAGCT GTCTGCATTC ACTGCATGAA CGAGTTCAGC CGGGGGAAGA 
     1981 ATGGGAAGGA CCTGGGCACG AGCTGCCTCA TCAGGCACAT GTGGAGGGCA CACCGCGCCA 
     2041 TCGTGTTGCA GGAGAACGGG GGCACGGGCA TCCCGCCACT GTACTCCACC CCTCCCACTC 
     2101 TGCTGCCTTC CTTGCTGCCG CCGGAGGGGG AGCTCAGCTC TGTGTCCTCG TCTCCGGTAA 
     2161 AGCCGGTCAG AGAGTCCCCT TCGGCCTCCT CCTCCCCTGA CAGGCTGACT GAGGACTTGC 
     2221 AGTCTCACTT GAACCCTGGA GATGGGCTGA TGGAAGACGT GGCGGCCTTC TCATCTTCCG 
     2281 ATGACGTAGG GGAGGCCTCG GCGTCCTCTC CTGAGAAGCA GCAGGCTGAT GGGCTGAGTC 
     2341 CTAGATTGTT TGAATCTGGT GCCATCTTCC AGCAGAATAA AAAGGTCATG AAAAGACTGA 
     2401 AGTCGGAGGT CTGGCATCAC TTCTCCCTGG CCCCCATGGA CAGCCTCAAG GCCGAGTGTC 
     2461 GGTACTGCGG CTGTGCCATC AGCCGGGGGA AGAAGGGTGA TGTGGGCACC AGCTGCCTGA 
     2521 TGAGACATCT CTACCGGCGC CATCCAGAAG TTGTCGGGAG CCAGAAGGGC TTCCTGGGTG 
     2581 CAAGTCTGGC AAACTCTCCG TATGCCACTT TGGCCTCTGC AGAAAGTTCC TCTTCCAAAT 
     2641 TGACTGACTT GCCAACAGTG GTCACAAAAA ACAATCAAGT TATGTTTCCT GTTAATAGCA 
     2701 AAAAGACCTC GAAGCTGTGG AATCATTTTT CTATTTGCTC CGCAGACTCC ACAAAAGTCG 
     2761 TGTGCTTGCA CTGTGGCCGG ACCATCAGCC GGGGGAAGAA GCCGACTAAC TTGGGTACCA 
     2821 GCTGTCTTCT GAGACATTTA CAGCGGTTCC ATAGCAACGT GCTGAAAACT GAGGTCTCGG 
     2881 AGACGGCTCG GCCCTCCTCT CCGGACACCC GGGTGCCGCG GGGCACAGAA TTATCAGGCG 
     2941 CTTCCTCTTT TGATGACACC AATGAGAAGT TTTACGATTC TCACCCAGTT GCCAAAAAAA 
     3001 TCACAAGTCT CATAGCTGAA ATGATTGCAC TTGACCTCCA GCCATATTCT TTTGTAGACA 
     3061 ACGTTGGCTT TAACAGGCTG CTTGAATACT TGAAACCTCA GTACTCCCTC CCCGCCCCTT 
     3121 CCTACTTCTC CAGGACAGCT ATCCCAGGTA TGTATGATAA TGTGAAGCAG ATAATTATGT 
     3181 CCCACCTTAA GGAAGCTGAG AGTGGTGTGA TCCACTTCAC GTCTGGAATA TGGATGAGTA 
     3241 ACCAGACCCG TGAGTACCTG ACCCTCACGG CCCACTGGGT TTCCTTCGAG TCGCCAGCCC 
     3301 GGCCGCGCTG TGACGACCAC CACTGCTCGG CGCTGTTGGA CGTGTCGCAG GTGGACTGCG 
     3361 ACTACAGTGG CAACAGCATT CAGAAGCAGC TGGAGTGCTG GTGGGAAGCG TGGGTGACCT 
     3421 CCACCGGCCT TCAGGTGGGC ATCACCGTCA CCGACAACGC CAGCATCGGG AAGACGCTGA 
     3481 ACGAGGGGGA GCACTCGAGC GTGCAGTGCT TCAGCCATAC GGTGAACCTG ATCGTCAGCG 
     3541 AGGCCATTAA GAGCCAGCGG ATGGTGCAGA ACCTGCTGAG CCTCGCCCGG AAGATCTGCG 
     3601 AGCGGGTGCA CCGGTCGCCC AAGGCGAAGG AGAAACTGGC CGAGCTGCAG AGGGAGTACG 
     3661 CGCTGCCTCA GCATCACCTC ATCCAGGACG TCCCGTCCAA GTGGAGCACT TCCTTCCACA 
     3721 TGCTCGAGCG GCTCATTGAG CAGAAAAGGG CCATTAACGA GATGTCCGTC GAGTGTAACT 
     3781 TCCGAGAGCT GATCAGCTGC GACCAGTGGG AGGTCATGCA GTCCGTGTGC CGTGCGCTAA 
     3841 AGCCCTTCGA GGCCGCGAGC CGGGAGATGA GCACGCAGAT GTCCACCCTC AGCCAGGTCA 
     3901 TCCCCATGGT ACACATCCTC AACAGGAAGG TGGAGATGCT CTTCGAGGAG ACGATGGGCA 
     3961 TCGACACCAT GCTGCGCTCT CTGAAGGAGG CCATGGTGAG CCGCCTGTCT GCCACCCTCC 
     4021 ACGACCCGCG GTACGTCTTC GCCACGCTGC TGGATCCTCG CTACAAGGCC TCCCTGTTTA 
     4081 CGGAGGAGGA GGCGGAGCAG TACAAACAGG ATTTAATCAG GGAACTCGAA CTCATGAATT 
     4141 CTACCTCAGA GGACGTGGCT GCCTCCCACA GGTGTGATGC TGGCTCCCCG TCGAAAGACT 
     4201 CTGCCGCAGA GGAGAACCTG TGGTCACTTG TGGCCAAGGT GAAGAAGAAA GACCCAAGAG 
     4261 AAAAGCTGCC TGAAGCCATG GTGCTTGCGT ATCTGGAGGA GGAGGTGCTT GAACACAGCT 
     4321 GTGACCCGCT CACCTACTGG AACCTGAAGA AGGCGTCCTG GCCGGGGCTG TCCGCGCTGG 
     4381 CCGTCAGATT TTTGGGCTGC CCCCCAAGCA TCGTCCCTTC AGAAAAGCTG TTCAACACAC 
     4441 CCACTGAGAA CGGTAGCCTT GGCCAATCCA GGCTCATGAT GGAACATTTT GAAAAACTTA 
     4501 TCTTTTTGAA AGTGAATCTT CCCTTAATAT ACTTTCAGTA TACGCGTACG CGGCCGCTCG 
     4561 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4621 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4681 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4741 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4801 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4861 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4921 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4981 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5041 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5101 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5161 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5221 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5281 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5341 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5401 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5461 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5521 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5581 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5641 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5701 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5761 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5821 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5881 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5941 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6001 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6061 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6121 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6181 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6241 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6301 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6361 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6421 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6481 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6541 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6601 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6661 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6721 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6781 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6841 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6901 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6961 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7021 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7081 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7141 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7201 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7261 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7321 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7381 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7441 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7501 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7561 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7621 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7801 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7861 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7921 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7981 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8041 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8101 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8161 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8221 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8281 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8341 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//