LOCUS       RC223909L2                8775 bp    DNA     circular  SYN 
DEFINITION  MBD4 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 4 (MBD4)
ACCESSION   NM_003925
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7066..7246 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7974..8633 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7294..7913 
                     /label="ColE1"  
     misc_feature    5941..5961 
                     /label="LR50"  
     misc_feature    5081..5806 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    5950..6541 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5060..5066 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5060 
                     /label="MBD4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGCACGACT GGGCTGGAGA GTCTGAGTCT 
     3361 GGGGGACCGC GGAGCTGCCC CCACCGTCAC CTCTAGTGAG CGCCTAGTCC CAGACCCGCC 
     3421 GAATGACCTC CGCAAAGAAG ATGTTGCTAT GGAATTGGAA AGAGTGGGAG AAGATGAGGA 
     3481 ACAAATGATG ATAAAAAGAA GCAGTGAATG TAATCCCTTG CTACAAGAAC CCATCGCTTC 
     3541 TGCTCAGTTT GGTGCTACTG CAGGAACAGA ATGCCGTAAG TCTGTCCCAT GTGGATGGGA 
     3601 AAGAGTTGTG AAGCAAAGGT TATTTGGGAA GACAGCAGGA AGATTTGATG TGTACTTTAT 
     3661 CAGCCCACAA GGACTGAAGT TCAGATCCAA AAGTTCACTT GCTAATTATC TTCACAAAAA 
     3721 TGGAGAGACT TCTCTTAAGC CAGAAGATTT TGATTTTACT GTACTTTCTA AAAGGGGTAT 
     3781 CAAGTCAAGA TATAAAGACT GCAGCATGGC AGCCCTGACA TCCCATCTAC AAAACCAAAG 
     3841 TAACAATTCA AACTGGAACC TCAGGACCCG AAGCAAGTGC AAAAAGGATG TGTTTATGCC 
     3901 GCCAAGTAGT AGTTCAGAGT TGCAGGAGAG CAGAGGACTC TCTAACTTTA CTTCCACTCA 
     3961 TTTGCTTTTG AAAGAAGATG AGGGTGTTGA TGATGTTAAC TTCAGAAAGG TTAGAAAGCC 
     4021 CAAAGGAAAG GTGACTATTT TGAAAGGAAT CCCAATTAAG AAAACTAAAA AAGGATGTAG 
     4081 GAAGAGCTGT TCAGGTTTTG TTCAAAGTGA TAGCAAAAGA GAATCTGTGT GTAATAAAGC 
     4141 AGATGCTGAA AGTGAACCTG TTGCACAAAA AAGTCAGCTT GATAGAACTG TCTGCATTTC 
     4201 TGATGCTGGA GCATGTGGTG AGACCCTCAG TGTGACCAGT GAAGAAAACA GCCTTGTAAA 
     4261 AAAAAAAGAA AGATCATTGA GTTCAGGATC AAATTTTTGT TCTGAACAAA AAACTTCTGG 
     4321 CATCATAAAC AAATTTTGTT CAGCCAAAGA CTCAGAACAC AACGAGAAGT ATGAGGATAC 
     4381 CTTTTTAGAA TCTGAAGAAA TCGGAACAAA AGTAGAAGTT GTGGAAAGGA AAGAACATTT 
     4441 GCATACTGAC ATTTTAAAAC GTGGCTCTGA AATGGACAAC AACTGCTCAC CAACCAGGAA 
     4501 AGACTTCACT GGTGAGAAAA TATTTCAAGA AGATACCATC CCACGAACAC AGATAGAAAG 
     4561 AAGGAAAACA AGCCTGTATT TTTCCAGCAA ATATAACAAA GAAGCTCTTA GCCCCCCACG 
     4621 ACGTAAAGCC TTTAAGAAAT GGACACCTCC TCGGTCACCT TTTAATCTCG TTCAAGAAAC 
     4681 ACTTTTTCAT GATCCATGGA AGCTTCTCAT CGCTACTATA TTTCTCAATC GGACCTCAGG 
     4741 CAAAATGGCA ATACCTGTGC TTTGGAAGTT TCTGGAGAAG TATCCTTCAG CTGAGGTAGC 
     4801 AAGAACCGCA GACTGGAGAG ATGTGTCAGA ACTTCTTAAA CCTCTTGGTC TCTACGATCT 
     4861 TCGGGCAAAA ACCATTGTCA AGTTCTCAGA TGAATACCTG ACAAAGCAGT GGAAGTATCC 
     4921 AATTGAGCTT CATGGGATTG GTAAATATGG CAACGACTCT TACCGAATTT TTTGTGTCAA 
     4981 TGAGTGGAAG CAGGTGCACC CTGAAGACCA CAAATTAAAT AAATATCATG ACTGGCTTTG 
     5041 GGAAAATCAT GAAAAATTAA GTCTATCTAC GCGTACGCGG CCGCTCGAGA TGAGCGGGGG 
     5101 CGAGGAGCTG TTCGCCGGCA TCGTGCCCGT GCTGATCGAG CTGGACGGCG ACGTGCACGG 
     5161 CCACAAGTTC AGCGTGCGCG GCGAGGGCGA GGGCGACGCC GACTACGGCA AGCTGGAGAT 
     5221 CAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTGG TGACCACCCT 
     5281 CTGCTACGGC ATCCAGTGCT TCGCCCGCTA CCCCGAGCAC ATGAAGATGA ACGACTTCTT 
     5341 CAAGAGCGCC ATGCCCGAGG GCTACATCCA GGAGCGCACC ATCCAGTTCC AGGACGACGG 
     5401 CAAGTACAAG ACCCGCGGCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA 
     5461 GCTGAAGGGC AAGGACTTCA AGGAGGACGG CAACATCCTG GGCCACAAGC TGGAGTACAG 
     5521 CTTCAACAGC CACAACGTGT ACATCCGCCC CGACAAGGCC AACAACGGCC TGGAGGCTAA 
     5581 CTTCAAGACC CGCCACAACA TCGAGGGCGG CGGCGTGCAG CTGGCCGACC ACTACCAGAC 
     5641 CAACGTGCCC CTGGGCGACG GCCCCGTGCT GATCCCCATC AACCACTACC TGAGCACTCA 
     5701 GACCAAGATC AGCAAGGACC GCAACGAGGC CCGCGACCAC ATGGTGCTCC TGGAGTCCTT 
     5761 CAGCGCCTGC TGCCACACCC ACGGCATGGA CGAGCTGTAC AGGTCCGGAC TCAGATAAGT 
     5821 TTAAACGGCC GGCCGCGGTC TGTACAAGTA GGATTCGTCG AGGGACCTAA TAACTTCGTA 
     5881 TAGCATACAT TATACGAAGT TATACATGTT TAAGGGTTCC GGTTCCACTA GGTACAATTC 
     5941 GATATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT 
     6001 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT 
     6061 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG 
     6121 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT 
     6181 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT 
     6241 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC 
     6301 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG 
     6361 TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC 
     6421 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG 
     6481 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC 
     6541 TCCCCGCATC GATACCGTCG ACCTCGATCG AGACCTAGAA AAACATGGAG CAATCACAAG 
     6601 TAGCAATACA GCAGCTACCA ATGCTGATTG TGCCTGGCTA GAAGCACAAG AGGAGGAGGA 
     6661 GGTGGGTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT 
     6721 AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG 
     6781 AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA GGCTACTTCC CTGATTGGCA 
     6841 GAACTACACA CCAGGGCCAG GGATCAGATA TCCACTGACC TTTGGATGGT GCTACAAGCT 
     6901 AGTACCAGTT GAGCAAGAGA AGGTAGAAGA AGCCAATGAA GGAGAGAACA CCCGCTTGTT 
     6961 ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA GAAGTATTAG AGTGGAGGTT 
     7021 TGACAGCCGC CTAGCATTTC ATCACATGGC CCGAGAGCTG CATCCGGACT GTACTGGGTC 
     7081 TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT 
     7141 TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA 
     7201 CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGCAT 
     7261 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     7321 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     7381 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     7441 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     7501 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     7561 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     7621 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     7681 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     7741 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     7801 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     7861 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     7921 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     7981 GATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG 
     8041 ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG 
     8101 TCGCCTTGCG TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG 
     8161 GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA 
     8221 TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC 
     8281 GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC 
     8341 GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC 
     8401 TCACCGTCTT TCATTGCCAT ACGGAACTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG 
     8461 TGAATAAAGG CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA 
     8521 ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA 
     8581 TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC 
     8641 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8701 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8761 AAAGTGCCAC CTGAC
//