LOCUS       RC223877                8481 bp    DNA     circular  SYN 
DEFINITION  NLRP5 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 5 (NLRP5)
ACCESSION   NM_153447
VERSION     NM_153447.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4638..4729 
                     /label="Myc-DDK"  
     misc_feature    4759..5344 
                     /label="PolyA signal"  
     misc_feature    4816..4835 
                     /label="Restriction Enzyme"  
     misc_feature    5620..6292 
                     /label="ColE1"  
     misc_feature    6722..7516 
                     /label="Kan/Neo"  
     misc_feature    7551..7853 
                     /label="SV40 ori"  
     misc_feature    7939..8416 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4620..4626 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4620 
                     /label="NLRP5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGTTGCA GGAGGACTTG AACTTGGAGC TGCTGCTCTG CTCTCAGCAT 
     1081 CACCACGTGC TCTTGTCACT CTTTCCACAG GTCCTACTTG CTCTATATTA CCAAAGAATC 
     1141 CACTTTTCCC CCAAAACCTG AGCTCTCAGC CTTGTATCAA GATGGAAGGA GACAAATCGC 
     1201 TCACCTTTTC CAGCTACGGG CTGCAATGGT GTCTCTATGA GCTAGACAAG GAAGAATTTC 
     1261 AGACATTCAA GGAATTACTA AAGAAGAAAT CTTCAGAATC GACCACATGC TCTATTCCAC 
     1321 AGTTTGAAAT CGAGAATGCC AACGTGGAAT GTCTGGCACT CCTCTTGCAT GAGTATTATG 
     1381 GAGCATCGCT GGCCTGGGCT ACGTCCATTA GCATCTTTGA AAACATGAAC CTGCGAACCC 
     1441 TCTCGGAGAA GGCACGGGAT GACATGAAAA GACATTCACC AGAAGATCCT GAAGCAACGA 
     1501 TGACTGACCA AGGACCAAGC AAGGAAAAAG TGCCAGGAAT TTCACAAGCT GTGCAACAAG 
     1561 ATAGTGCCAC AGCTGCAGAG ACAAAAGAAC AAGAAATTTC ACAAGCTATG GAACAAGAAG 
     1621 GTGCCACAGC AGCAGAGACA GAAGAACAAG AAATTTCACA AGCTATGGAA CAAGAAGGTG 
     1681 CCACAGCAGC AGAGACAGAA GAACAAGGAC ATGGAGGTGA CACATGGGAC TACAAGAGTC 
     1741 ACGTGATGAC CAAATTCGCT GAGGAGGAGG ATGTACGTCG TAGTTTTGAA AACACTGCTG 
     1801 CTGACTGGCC GGAAATGCAA ACGTTGGCTG GTGCTTTTGA TTCAGACCGG TGGGGCTTCC 
     1861 GGCCTCGCAC GGTGGTTCTG CACGGAAAGT CAGGAATTGG GAAATCGGCT CTAGCCAGAA 
     1921 GGATCGTGCT GTGCTGGGCG CAAGGTGGAC TCTACCAGGG AATGTTCTCC TACGTCTTCT 
     1981 TCCTCCCCGT TAGAGAGATG CAGCGGAAGA AGGAGAGCAG TGTCACAGAG TTCATCTCCA 
     2041 GGGAGTGGCC AGACTCCCAG GCTCCGGTGA CGGAGATCAT GTCCCGACCA GAAAGGCTGT 
     2101 TGTTCATCAT TGACGGTTTC GATGACCTGG GCTCTGTCCT CAACAATGAC ACAAAGCTCT 
     2161 GCAAAGACTG GGCTGAGAAG CAGCCTCCGT TCACCCTCAT ACGCAGTCTG CTGAGGAAGG 
     2221 TCCTGCTCCC TGAGTCCTTC CTGATCGTCA CCGTCAGAGA CGTGGGCACA GAGAAGCTCA 
     2281 AGTCAGAGGT CGTGTCTCCC CGTTACCTGT TAGTTAGAGG AATCTCCGGG GAACAAAGAA 
     2341 TCCACTTGCT CCTTGAGCGC GGGATTGGTG AGCATCAGAA GACACAAGGG TTGCGTGCGA 
     2401 TCATGAACAA CCGTGAGCTG CTCGACCAGT GCCAGGTGCC CGCCGTGGGC TCTCTCATCT 
     2461 GCGTGGCCCT GCAGCTGCAG GACGTGGTGG GGGAGAGCGT CGCCCCCTTC AACCAAACGC 
     2521 TCACAGGCCT GCACGCCGCT TTTGTGTTTC ATCAGCTCAC CCCTCGAGGC GTGGTCCGGC 
     2581 GCTGTCTCAA TCTGGAGGAA AGAGTTGTCC TGAAGCGCTT CTGCCGTATG GCTGTGGAGG 
     2641 GAGTGTGGAA TAGGAAGTCA GTGTTTGACG GTGACGACCT CATGGTTCAA GGACTCGGGG 
     2701 AGTCTGAGCT CCGTGCTCTG TTTCACATGA ACATCCTTCT CCCAGACAGC CACTGTGAGG 
     2761 AGTACTACAC CTTCTTCCAC CTCAGTCTCC AGGACTTCTG TGCCGCCTTG TACTACGTGT 
     2821 TAGAGGGCCT GGAAATCGAG CCAGCTCTCT GCCCTCTGTA CGTTGAGAAG ACAAAGAGGT 
     2881 CCATGGAGCT TAAACAGGCA GGCTTCCATA TCCACTCGCT TTGGATGAAG CGTTTCTTGT 
     2941 TTGGCCTCGT GAGCGAAGAC GTAAGGAGGC CACTGGAGGT CCTGCTGGGC TGTCCCGTTC 
     3001 CCCTGGGGGT GAAGCAGAAG CTTCTGCACT GGGTCTCTCT GTTGGGTCAG CAGCCTAATG 
     3061 CCACCACCCC AGGAGACACC CTGGACGCCT TCCACTGTCT TTTCGAGACT CAAGACAAAG 
     3121 AGTTTGTTCG CTTGGCATTA AACAGCTTCC AAGAAGTGTG GCTTCCGATT AACCAGAACC 
     3181 TGGACTTGAT AGCATCTTCC TTCTGCCTCC AGCACTGTCC GTATTTGCGG AAAATTCGGG 
     3241 TGGATGTCAA AGGGATCTTC CCAAGAGATG AGTCCGCTGA GGCATGTCCT GTGGTCCCTC 
     3301 TATGGATGCG GGATAAGACC CTCATTGAGG AGCAGTGGGA AGATTTCTGC TCCATGCTTG 
     3361 GCACCCACCC ACACCTGCGG CAGCTGGACC TGGGCAGCAG CATCCTGACA GAGCGGGCCA 
     3421 TGAAGACCCT GTGTGCCAAG CTGAGGCATC CCACCTGCAA GATACAGACC CTGATGTTTA 
     3481 GAAATGCACA GATTACCCCT GGTGTGCAGC ACCTCTGGAG AATCGTCATG GCCAACCGTA 
     3541 ACCTAAGATC CCTCAACTTG GGAGGCACCC ACCTGAAGGA AGAGGATGTA AGGATGGCGT 
     3601 GTGAAGCCTT AAAACACCCA AAATGTTTGT TGGAGTCTTT GAGGCTGGAT TGCTGTGGAT 
     3661 TGACCCATGC CTGTTACCTG AAGATCTCCC AAATCCTTAC GACCTCCCCC AGCCTGAAAT 
     3721 CTCTGAGCCT GGCAGGAAAC AAGGTGACAG ACCAGGGAGT AATGCCTCTC AGTGATGCCT 
     3781 TGAGAGTCTC CCAGTGCGCC CTGCAGAAGC TGATACTGGA GGACTGTGGC ATCACAGCCA 
     3841 CGGGTTGCCA GAGTCTGGCC TCAGCCCTCG TCAGCAACCG GAGCTTGACA CACCTGTGCC 
     3901 TATCCAACAA CAGCCTGGGG AACGAAGGTG TAAATCTACT GTGTCGATCC ATGAGGCTTC 
     3961 CCCACTGTAG TCTGCAGAGG CTGATGCTGA ATCAGTGCCA CCTGGACACG GCTGGCTGTG 
     4021 GTTTTCTTGC ACTTGCGCTT ATGGGTAACT CATGGCTGAC GCACCTGAGC CTTAGCATGA 
     4081 ACCCTGTGGA AGACAATGGC GTGAAGCTTC TGTGCGAGGT CATGAGAGAA CCATCTTGTC 
     4141 ATCTCCAGGA CCTGGAGTTG GTAAAGTGTC ATCTCACCGC CGCGTGCTGT GAGAGTCTGT 
     4201 CCTGTGTGAT CTCGAGGAGC AGACACCTGA AGAGCCTGGA TCTCACGGAC AATGCCCTGG 
     4261 GTGACGGTGG GGTTGCTGCG CTGTGCGAGG GACTGAAGCA AAAGAACAGT GTTCTGGCGA 
     4321 GACTCGGGTT GAAGGCATGT GGACTGACTT CTGATTGCTG TGAGGCACTC TCCTTGGCCC 
     4381 TTTCCTGCAA CCGGCATCTG ACCAGTCTAA ACCTGGTGCA GAATAACTTC AGTCCCAAAG 
     4441 GAATGATGAA GCTGTGTTCG GCCTTTGCCT GTCCCACGTC TAACTTACAG ATAATTGGGC 
     4501 TGTGGAAATG GCAGTACCCT GTGCAAATAA GGAAGCTGCT GGAGGAAGTG CAGCTACTCA 
     4561 AGCCCCGAGT CGTAATTGAC GGTAGTTGGC ATTCTTTTGA TGAAGATGAC CGGTACTGGT 
     4621 GGAAAAACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4681 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4741 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4801 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4861 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4921 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4981 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5041 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5101 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5161 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5221 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5281 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5341 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5401 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5461 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5521 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5581 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5641 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5701 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5761 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5821 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5881 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5941 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6001 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6061 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6121 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6181 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6241 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6301 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6361 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6421 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6481 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6541 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6601 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6661 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6721 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6781 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6841 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6901 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6961 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7021 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7081 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7141 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7201 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7261 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7321 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7381 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7441 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7501 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7561 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7621 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7681 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7921 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7981 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8041 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8101 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8161 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8221 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8281 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8341 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8401 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8461 CAAAAATTTA ACGCGAATTT T
//