LOCUS       RC223826                6915 bp    DNA     circular  SYN 
DEFINITION  CUX1 (Myc-DDK-tagged)-Human cut-like homeobox 1 (CUX1), transcript variant 2
ACCESSION   NM_001913
VERSION     NM_001913.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3072..3163 
                     /label="Myc-DDK"  
     misc_feature    3193..3778 
                     /label="PolyA signal"  
     misc_feature    3250..3269 
                     /label="Restriction Enzyme"  
     misc_feature    4054..4726 
                     /label="ColE1"  
     misc_feature    5156..5950 
                     /label="Kan/Neo"  
     misc_feature    5985..6287 
                     /label="SV40 ori"  
     misc_feature    6373..6850 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3054..3060 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3054 
                     /label="CUX1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCAAT GTGGGATCGA TGTTTCAATA TTGGAAGCGC TTTGATTTAC 
     1081 AGCAGCTGCA GAGAGAACTC GATGCCACCG CAACGGTATT GGCGAACCGG CAGGATGAAA 
     1141 GTGAGCAGTC CAGAAAGCGG CTTATCGAAC AGAGCCGGGA GTTCAAGAAG AACACTCCAG 
     1201 AGGATTTGCG CAAGCAGGTA GCGCCGCTGC TGAAGAGTTT CCAAGGAGAG ATTGATGCAC 
     1261 TGAGTAAAAG AAGCAAGGAA GCTGAAGCAG CTTTCTTGAA TGTCTACAAA AGATTGATTG 
     1321 ACGTCCCAGA TCCCGTACCA GCTTTGGATC TCGGACAGCA ACTCCAGCTC AAAGTGCAGC 
     1381 GCCTGCACGA TATTGAAACA GAGAACCAGA AACTTAGGGA AACTCTGGAA GAATACAACA 
     1441 AGGAATTTGC TGAAGTGAAA AATCAAGAGG TTACGATAAA AGCACTTAAA GAGAAAATCC 
     1501 GAGAATATGA ACAGACACTG AAGAACCAAG CCGAAACCAT AGCTCTTGAG AAGGAACAGA 
     1561 AGTTACAGAA TGACTTTGCA GAAAAGGAGA GAAAGCTGCA GGAGACACAG ATGTCCACCA 
     1621 CCTCAAAGCT GGAGGAAGCT GAGCATAAGG TTCAGAGCCT ACAAACAGCC CTGGAAAAAA 
     1681 CTCGAACAGA ATTATTTGAC CTGAAAACCA AATACGATGA AGAAACTACT GCAAAGGCCG 
     1741 ACGAGATTGA AATGATCATG ACGGACCTTG AAAGGGCAAA CCAGAGGGCA GAGGTGGCTC 
     1801 AGAGAGAGGC GGAGACCTTA AGGGAACAGC TCTCATCGGC CAATCACTCC CTCCAGCTGG 
     1861 CCTCACAGAT CCAGAAGGCA CCAGACGTGG AGCAGGCCAT AGAGGTGCTG ACCCGCTCCA 
     1921 GCCTAGAAGT TGAGTTGGCC GCCAAGGAGC GGGAGATCGC ACAGCTGGTG GAGGACGTGC 
     1981 AGAGACTCCA GGCCAGCCTC ACCAAGCTGC GGGAGAATTC GGCCAGCCAG ATCTCACAGC 
     2041 TTGAGCAGCA GCTGAGCGCC AAAAACAGCA CACTCAAACA ACTGGAAGAA AAACTCAAAG 
     2101 GCCAGGCTGA CTATGAAGAG GTGAAGAAAG AGCTGAACAT TCTGAAGTCC ATGGAGTTTG 
     2161 CACCGTCCGA GGGCGCTGGG ACACAGGATG CGGCCAAGCC CCTGGAGGTG CTGTTGCTGG 
     2221 AGAAGAACCG CTCGCTGCAG TCCGAGAACG CCGCGCTGCG CATCTCCAAC AGCGACCTGA 
     2281 GCGGACGCTG TGCAGAGCTG CAGGTCCGTA TCACTGAGGC TGTGGCCACA GCCACTGAGC 
     2341 AGAGAGAGCT GATCGCCCGC CTGGAGCAGG ACCTGAGCAT CATTCAGTCC ATCCAGCGGC 
     2401 CCGATGCCGA GGGTGCCGCT GAGCACCGCC TGGAGAAGAT CCCAGAGCCC ATCAAAGAGG 
     2461 CCACTGCCCT ATTCTACGGA CCTGCAGCAC CAGCCAGCGG TGCCCTCCCA GAGGGCCAGG 
     2521 TGGATTCACT GCTTTCCATC ATCTCCAGCC AGAGGGAGCG CTTCCGTGCC CGGAACCAGG 
     2581 AGCTTGAGGC CGAGAACCGC CTGGCCCAGC ACACCCTCCA GGCCCTGCAG AGTGAGCTGG 
     2641 ACAGCCTGCG CGCCGACAAC ATCAAGCTCT TTGAGAAGAT CAAGTTCCTG CAGAGCTACC 
     2701 CTGGCCGGGG CAGCGGCAGT GATGACACGG AGCTGCGGTA CTCGTCCCAG TACGAGGAGC 
     2761 GCCTGGACCC CTTCTCCTCC TTCAGCAAGC GGGAGCGGCA GAGGAAGTAC CTGAGCTTGA 
     2821 GTCCCTGGGA CAAGGCCACC CTCAGCATGG GGCGTCTGGT TCTCTCCAAC AAGATGGCGC 
     2881 GCACCATCGG CTTCTTCTAC ACACTGTTCC TGCACTGCCT GGTCTTCCTG GTGCTCTACA 
     2941 AGCTGGCATG GAGCGAGAGC ATGGAGAGGG ACTGTGCCAC CTTCTGCGCC AAGAAGTTCG 
     3001 CTGACCACCT GCACAAGTTC CACGAGAATG ACAACGGGGC TGCGGCTGGT GACTTGTGGC 
     3061 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3121 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3181 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3241 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3301 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3361 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3421 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3481 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3541 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3601 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3661 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3721 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     3781 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     3841 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     3901 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     3961 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4021 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4081 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4141 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4201 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4261 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4321 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4381 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4441 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4501 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4561 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4621 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     4681 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     4741 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     4801 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     4861 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     4921 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     4981 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5041 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5101 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5161 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5221 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5281 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5341 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5401 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5461 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5521 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5581 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     5641 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     5701 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     5761 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     5821 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     5881 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     5941 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6001 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6061 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6121 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6181 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6241 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6301 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6361 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6421 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6481 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6541 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6601 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     6661 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     6721 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     6781 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     6841 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     6901 TTTAACGCGA ATTTT
//