LOCUS       RC223811                8463 bp    DNA     circular  SYN 
DEFINITION  PLCB4 (Myc-DDK-tagged)-Human phospholipase C, beta 4 (PLCB4), transcript variant 1
ACCESSION   NM_000933
VERSION     NM_000933.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4620..4711 
                     /label="Myc-DDK"  
     misc_feature    4741..5326 
                     /label="PolyA signal"  
     misc_feature    4798..4817 
                     /label="Restriction Enzyme"  
     misc_feature    5602..6274 
                     /label="ColE1"  
     misc_feature    6704..7498 
                     /label="Kan/Neo"  
     misc_feature    7533..7835 
                     /label="SV40 ori"  
     misc_feature    7921..8398 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4602..4608 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4602 
                     /label="PLCB4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAAACCT TATGAATTTA ACTGGCAGAA GGAAGTTCCC TCCTTTTTGC 
     1081 AAGAAGGAGC AGTTTTTGAC AGATACGAGG AGGAATCCTT TGTGTTTGAA CCCAACTGCC 
     1141 TCTTCAAAGT GGATGAGTTT GGCTTCTTTC TGACATGGAG AAGTGAAGGC AAGGAAGGAC 
     1201 AGGTGCTAGA ATGCTCCCTC ATCAACAGTA TTCGGTCGGG AGCCATACCA AAGGATCCCA 
     1261 AAATCTTGGC TGCTCTTGAA GCTGTTGGAA AATCAGAAAA TGATCTGGAA GGGCGGATAG 
     1321 TTTGTGTCTG CAGTGGCACA GATCTAGTGA ACATTAGTTT TACCTACATG GTGGCTGAAA 
     1381 ATCCAGAAGT AACTAAGCAA TGGGTAGAAG GCCTGAGATC AATCATACAC AACTTCAGGG 
     1441 CCAACAACGT CAGTCCAATG ACATGCCTCA AGAAACACTG GATGAAATTG GCATTTATGA 
     1501 CCAACACAAA TGGTAAAATT CCAGTTAGGA GTATTACTAG AACATTTGCA TCGGGAAAAA 
     1561 CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGTTAGGTCT TCCCAGTGGA AAGAATGATG 
     1621 AAATTGAGCC CACAGCATTT TCTTATGAAA AGTTCTATGA ACTGACACAA AAGATTTGTC 
     1681 CTCGGACAGA TATAGAAGAT CTTTTCAAAA AAATCAATGG AGACAAAACT GATTATTTAA 
     1741 CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA TTGAATGAAA 
     1801 TTTTATTTCC ATTTTATGAT GCCAAAAGGG CAATGCAGAT CATTGAGATG TATGAACCTG 
     1861 ATGAAGATTT GAAGAAAAAA GGCCTTATAT CAAGTGATGG GTTTTGCAGA TATCTGATGT 
     1921 CAGATGAAAA CGCCCCAGTC TTCCTAGATC GTTTAGAACT TTACCAAGAA ATGGACCATC 
     1981 CTCTGGCTCA CTACTTCATC AGTTCTTCCC ATAACACTTA TCTCACTGGC AGACAGTTCG 
     2041 GCGGGAAGTC TTCGGTAGAA ATGTACAGAC AGGTTCTCCT GGCTGGTTGC AGATGTGTTG 
     2101 AACTTGACTG CTGGGATGGA AAAGGTGAAG ACCAAGAACC AATAATAACT CATGGAAAAG 
     2161 CAATGTGTAC AGATATCCTT TTTAAGGATG TAATTCAAGC CATCAAGGAA ACTGCATTTG 
     2221 TCACATCAGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA TATCAACAGT 
     2281 ACAAGATGTC CAAATATTGC GAAGATCTAT TTGGGGATCT CCTGTTGAAA CAAGCACTTG 
     2341 AATCACATCC ACTTGAACCA GGCAGGGCTT TGCCATCCCC CAATGACCTC AAAAGAAAAA 
     2401 TACTCATAAA AAACAAGCGG CTGAAACCTG AAGTTGAAAA AAAACAGCTG GAAGCTTTGA 
     2461 GAAGCATGAT GGAAGCTGGA GAATCTGCCT CCCCAGCAAA CATCTTAGAG GACGATAATG 
     2521 AAGAGGAGAT CGAAAGTGCT GACCAAGAGG AGGAAGCTCA CCCCGAATTC AAATTTGGAA 
     2581 ATGAACTTTC TGCTGATGAC TTGGGTCACA AGGAAGCTGT TGCAAATAGC GTCAAGAAGG 
     2641 GCCTGGTCAC TGTAGAAGAT GAGCAGGCGT GGATGGCATC TTATAAATAT GTAGGTGCTA 
     2701 CCACTAATAT CCATCCATAT TTGTCCACAA TGATCAACTA CGCCCAGCCT GTAAAGTTTC 
     2761 AAGGTTTCCA TGTGGCAGAA GAACGCAATA TTCATTATAA CATGTCTTCT TTTAATGAAT 
     2821 CAGTCGGTCT TGGCTACTTG AAGACACATG CAATTGAATT TGTCAATTAT AACAAACGGC 
     2881 AAATGAGTCG CATTTACCCC AAGGGAGGCC GAGTCGATTC CAGTAATTAC ATGCCTCAGA 
     2941 TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CACTGAACTA TCAAACCCCA GATTTAGCGA 
     3001 TGCAATTGAA TCAGGGAAAA TTTGAGTATA ATGGATCGTG CGGGTACCTT CTCAAACCAG 
     3061 ATTTCATGAG GCGGCCTGAT CGAACATTTG ACCCCTTCTC TGAAACTCCT GTTGATGGTG 
     3121 TTATTGCAGC CACTTGCTCA GTGCAGGTTA TATCAGGTCA ATTCTTATCA GATAAGAAAA 
     3181 TTGGCACCTA CGTAGAGGTG GATATGTATG GGTTGCCCAC TGACACCATA CGTAAGGAAT 
     3241 TCCGAACTCG CATGGTTATG AATAATGGAC TCAATCCAGT TTACAATGAA GAGTCATTTG 
     3301 TATTTCGGAA GGTGATCCTG CCGGACCTGG CTGTCTTGAG AATAGCTGTG TATGATGATA 
     3361 ACAACAAGCT GATTGGCCAG AGGATCCTCC CGCTTGATGG CCTCCAAGCC GGATATCGAC 
     3421 ACATTTCCCT TCGAAATGAG GGAAATAAAC CATTATCACT ACCAACAATT TTCTGCAATA 
     3481 TTGTTCTTAA AACATATGTG CCTGATGGAT TTGGAGATAT CGTGGATGCT TTATCAGATC 
     3541 CAAAGAAATT TCTCTCAATT ACAGAAAAGA GAGCAGACCA AATGAGAGCT ATGGGCATTG 
     3601 AAACTAGTGA CATAGCCGAC GTGCCCAGTG ACACTTCCAA AAATGACAAG AAAGGAAAGG 
     3661 CCAACACCGC CAAAGCAAAT GTGACCCCTC AGAGTAGCTC TGAGCTCAGA CCAACCACCA 
     3721 CGGCTGCCCT GGCCTCTGGT GTGGAAGCCA AGAAAGGTAT TGAACTTATC CCTCAAGTAA 
     3781 GGATAGAAGA CTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA CAGCAGAAGG 
     3841 AGCTAAATTC TTTAAAGAAG AAACATGCAA AGGAACACAG TACCATGCAG AAGTTACACT 
     3901 GCACGCAAGT TGACAAAATT GTGGCACAGT ATGACAAAGA GAAGTCGACT CATGAGAAAA 
     3961 TCCTAGAGAA GGCAATGAAG AAGAAGGGGG GAAGTAATTG TCTCGAAATG AAAAAAGAAA 
     4021 CAGAAATCAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAA GAGATTGTAG 
     4081 CACAGCACAC AAAGGAATGG TCAGAAATGA TCAATACCCA CAGTGCTGAG GAGCAAGAAA 
     4141 TCCGAGACCT GCACCTCAGC CAGCAGTGTG AGCTGCTGAA AAAGCTACTC ATCAATGCCC 
     4201 ACGAGCAGCA AACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAG GAAATGCGAG 
     4261 CACACCAGGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT AAATCTATCA 
     4321 AGAATAAAGC AGAACGGGAA AGGCGAGTCA GGGAGTTAAA CAGCAGCAAC ACTAAAAAGT 
     4381 TTCTGGAAGA AAGAAAGAGA CTTGCCATGA AGCAGTCCAA AGAAATGGAT CAGTTGAAAA 
     4441 AAGTCCAGCT TGAACATCTA GAATTCCTAG AGAAACAGAA TGAGCAGCTT TTGAAATCCT 
     4501 GTCATGCAGT GTCCCAAACG CAAGGCGAAG GAGATGCAGC AGATGGTGAA ATTGGAAGCC 
     4561 GAGATGGACC GCAGACCAGC AACAGTAGTA TGAAACTCCA AAATGCAAAC ACGCGTACGC 
     4621 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4681 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4741 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4801 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4861 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4921 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4981 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5041 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5101 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5161 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5221 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5281 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5341 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5401 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5461 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5521 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5581 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5641 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5701 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5761 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5821 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5881 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5941 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6001 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6061 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6121 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6181 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6241 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6301 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6361 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6421 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6481 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6541 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6601 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6661 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6721 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6781 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6841 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6901 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6961 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7021 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7081 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7141 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7201 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7261 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7321 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7381 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7441 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7501 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7561 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7621 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7681 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7741 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7801 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7861 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7921 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7981 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8041 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8101 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8161 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8221 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8281 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8341 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8401 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8461 TTT
//