LOCUS       RC223783                9606 bp    DNA     circular  SYN 
DEFINITION  IQGAP2 (Myc-DDK-tagged)-Human IQ motif containing GTPase activating protein 2 (IQGAP2)
ACCESSION   NM_006633
VERSION     NM_006633.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5763..5854 
                     /label="Myc-DDK"  
     misc_feature    5884..6469 
                     /label="PolyA signal"  
     misc_feature    5941..5960 
                     /label="Restriction Enzyme"  
     misc_feature    6745..7417 
                     /label="ColE1"  
     misc_feature    7847..8641 
                     /label="Kan/Neo"  
     misc_feature    8676..8978 
                     /label="SV40 ori"  
     misc_feature    9064..9541 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5745..5751 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5745 
                     /label="IQGAP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACACGAA GAGCTGCCGT CGCTGCAGAG ACCCCGCTAT GGCTCTATTG 
     1081 TGGACGATGA AAGGCTCTCT GCAGAGGAGA TGGATGAGAG GAGGCGGCAG AACATTGCTT 
     1141 ATGAATATCT GTGCCACTTA GAGGAAGCCA AAAGGTGGAT GGAAGTTTGC TTAGTTGAAG 
     1201 AATTGCCACC AACCACTGAA TTGGAAGAAG GGCTCCGGAA TGGAGTTTAC CTTGCAAAGT 
     1261 TAGCCAAGTT CTTTGCCCCG AAAATGGTAT CAGAGAAAAA GATCTATGAT GTGGAACAAA 
     1321 CACGTTATAA GAAGTCTGGC CTTCATTTTC GACACACAGA TAATACCGTC CAGTGGTTAA 
     1381 GAGCGATGGA GTCTATTGGT CTACCCAAGA TATTTTATCC AGAAACAACA GATGTCTATG 
     1441 ATCGGAAAAA CATACCAAGA ATGATATATT GCATTCACGC ACTGAGTTTG TATCTGTTCA 
     1501 AACTAGGAAT AGCACCCCAG ATCCAGGATT TGTTGGGCAA AGTAGACTTC ACAGAGGAGG 
     1561 AAATCAGTAA TATGAGAAAA GAACTTGAGA AATATGGAAT ACAGATGCCA TCTTTCAGCA 
     1621 AAATAGGTGG TATTCTGGCC AATGAACTGT CCGTGGATGA AGCTGCATTA CATGCTGCAG 
     1681 TTATAGCCAT TAATGAAGCA GTTGAAAAAG GAATAGCAGA GCAAACCGTT GTAACACTAA 
     1741 GAAACCCAAA TGCGGTTTTA ACTTTAGTGG ATGACAACCT TGCACCAGAA TATCAGAAAG 
     1801 AACTCTGGGA TGCCAAAAAG AAAAAAGAGG AAAATGCAAG ACTGAAGAAT AGCTGTATTT 
     1861 CAGAAGAAGA AAGAGATGCT TATGAAGAAC TGCTGACACA AGCAGAAATC CAAGGCAATA 
     1921 TTAATAAAGT CAACAGGCAG GCTGCAGTGG ACCATATCAA TGCTGTCATT CCGGAAGGTG 
     1981 ACCCCGAGAA TACGCTGCTT GCACTGAAGA AACCAGAGGC CCAGCTGCCT GCTGTTTATC 
     2041 CCTTTGCTGC TGCCATGTAT CAGAACGAAC TTTTCAACCT CCAGAAACAG AACACCATGA 
     2101 ACTACTTGGC CCACGAGGAG CTTTTGATTG CTGTGGAAAT GTTGTCTGCT GTTGCTTTAC 
     2161 TAAACCAGGC CTTGGAAAGC AACGATCTTG TGTCTGTGCA GAATCAACTC AGAAGCCCCG 
     2221 CAATAGGCTT AAACAATCTG GACAAGGCAT ATGTGGAACG TTATGCAAAC ACACTACTCT 
     2281 CTGTTAAACT AGAAGTTTTA TCCCAAGGGC AAGATAACTT AAGCTGGAAT GAAATTCAGA 
     2341 ATTGTATTGA TATGGTTAAT GCTCAAATTC AAGAAGAAAA TGACCGAGTT GTAGCTGTAG 
     2401 GGTACATCAA TGAAGCTATT GATGAAGGGA ATCCTTTGAG GACTTTAGAA ACTTTGCTCC 
     2461 TACCTACTGC GAATATTAGT GATGTGGACC CAGCCCATGC CCAGCACTAC CAGGATGTTT 
     2521 TATACCATGC TAAATCACAG AAACTCGGAG ACTCTGAGAG TGTTTCCAAA GTGCTTTGGC 
     2581 TGGATGAGAT ACAGCAAGCC GTCGATGAGG CCAACGTGGA CGAGGACAGA GCAAAACAAT 
     2641 GGGTTACTCT GGTGGTTGAT GTTAATCAGT GTTTGGAAGG AAAAAAATCA AGTGATATTT 
     2701 TGTCTGTATT GAAGTCTTCC ACTTCTAATG CAAATGACAT AATCCCGGAG TGTGCTGACA 
     2761 AATACTATGA TGCCCTTGTG AAGGCAAAAG AGCTCAAATC TGAAAGAGTG TCTAGTGACG 
     2821 GTTCATGGCT CAAACTCAAC CTGCACAAAA AATATGACTA CTATTACAAC ACTGATTCAA 
     2881 AAGAGAGTTC CTGGGTCACA CCTGAATCAT GCTTCTATAA AGAATCATGG CTCACAGGAA 
     2941 AAGAAATCGA GGACATTATT GAGGAAGTCA CAGTAGGTTA CATTCGTGAG AATATATGGT 
     3001 CTGCTTCAGA AGAGTTGCTT CTTCGCTTTC AAGCCACAAG CTCAGGACCC ATCCTTAGGG 
     3061 AAGAGTTTGA AGCTAGAAAA TCATTTTTGC ATGAACAAGA AGAGAATGTG GTCAAAATAC 
     3121 AGGCTTTTTG GAAAGGATAT AAACAACGGA AGGAGTATAT GCACAGGCGG CAAACGTTCA 
     3181 TTGATAATAC TGATTCTGTT GTGAAGATTC AGTCCTGGTT CCGAATGGCA ACTGCAAGAA 
     3241 AGAGCTATCT TTCAAGACTA CAGTATTTCA GAGATCATAA TAATGAAATT GTGAAAATAC 
     3301 AGTCACTGTT GAGAGCGAAC AAAGCTAGAG ATGACTACAA AACATTGGTT GGCTCTGAAA 
     3361 ACCCACCATT AACAGTAATT CGCAAATTTG TATACCTGCT GGACCAAAGT GATTTGGATT 
     3421 TCCAGGAGGA ACTAGAGGTT GCACGATTAA GGGAAGAAGT AGTGACCAAG ATCAGGGCCA 
     3481 ATCAACAGCT GGAAAAAGAC CTGAACCTGA TGGACATCAA GATTGGACTG CTGGTGAAGA 
     3541 ACAGGATCAC ACTAGAGGAT GTAATTTCAC ACAGTAAAAA GCTGAACAAG AAAAAAGGAG 
     3601 GAGAAATGGA AATACTGAAT AACACCGACA ACCAAGGAAT AAAAAGTTTG AGTAAGGAGA 
     3661 GGAGAAAAAC ACTAGAAACA TATCAGCAGC TGTTTTACCT TTTACAGACC AACCCTTTAT 
     3721 ACTTGGCTAA GCTGATTTTC CAGATGCCAC AGAACAAGTC CACTAAATTT ATGGATACTG 
     3781 TTATTTTCAC ACTATATAAT TATGCCTCTA ATCAGCGAGA AGAATATCTA CTTCTCAAGC 
     3841 TTTTTAAAAC TGCTCTGGAG GAAGAAATAA AATCAAAAGT GGACCAGGTA CAGGACATAG 
     3901 TTACTGGTAA CCCTACAGTC ATCAAGATGG TCGTCAGCTT CAATAGAGGT GCCCGGGGAC 
     3961 AGAACACCCT GCGCCAACTC CTGGCTCCAG TGGTAAAAGA GATCATCGAC GACAAGTCGC 
     4021 TGATTATCAA CACAAACCCT GTAGAGGTGT ACAAGGCTTG GGTGAACCAA CTAGAAACAC 
     4081 AGACTGGAGA GGCCAGCAAG TTGCCTTATG ATGTGACCAC AGAACAAGCT CTAACATACC 
     4141 CAGAAGTGAA AAATAAACTG GAGGCTTCCA TTGAGAACCT GAGAAGGGTC ACCGACAAAG 
     4201 TCCTGAATTC TATCATTTCT TCCCTTGATC TACTGCCTTA TGGATTGAGG TATATAGCCA 
     4261 AAGTACTGAA GAATTCGATC CATGAGAAAT TCCCCGATGC AACAGAAGAT GAGCTATTAA 
     4321 AGATTGTTGG AAACCTCCTG TACTATCGGT ACATGAATCC AGCCATTGTA GCTCCAGATG 
     4381 GCTTTGATAT CATCGACATG ACAGCTGGAG GTCAGATAAA TTCTGACCAA AGGAGAAACT 
     4441 TAGGATCAGT GGCCAAGGTT CTTCAGCACG CAGCCTCCAA CAAGCTGTTT GAAGGAGAAA 
     4501 ATGAGCATCT CTCATCTATG AACAATTATT TATCAGAGAC GTATCAGGAA TTCAGGAAAT 
     4561 ATTTCAAAGA AGCATGTAAT GTCCCTGAGC CAGAAGAGAA GTTTAATATG GACAAATACA 
     4621 CAGACCTGGT GACAGTCAGC AAACCAGTCA TTTATATTTC AATTGAAGAA ATCATCAGCA 
     4681 CACACTCACT CCTGTTGGAA CACCAGGATG CAATTGCCCC TGAGAAAAAT GACTTACTGA 
     4741 GTGAATTGCT GGGGTCGCTG GGAGAGGTGC CAACCGTGGA ATCTTTTCTT GGGGAAGGAG 
     4801 CAGTTGACCC CAATGACCCT AACAAGGCAA ATACACTAAG TCAGCTTTCA AAGACCGAGA 
     4861 TTTCTCTTGT CTTGACAAGC AAATATGACA TAGAGGACGG TGAAGCTATA GATAGCCGAA 
     4921 GCCTCATGAT AAAGACCAAG AAGCTGATAA TTGATGTGAT CCGGAACCAG CCAGGGAACA 
     4981 CATTGACAGA AATCTTAGAG ACACCAGCAA CTGCGCAACA GGAGGTAGAC CATGCCACGG 
     5041 ACATGGTGAG CCGTGCAATG ATAGATTCCA GGACTCCAGA AGAAATGAAG CATAGCCAAT 
     5101 CTATGATTGA AGATGCACAG CTGCCTCTTG AGCAGAAGAA GAGGAAAATC CAGAGGAATC 
     5161 TTCGGACGTT GGAACAGACT GGACACGTGT CATCCGAAAA TAAATACCAA GACATTCTCA 
     5221 ATGAGATTGC CAAGGATATT CGAAATCAAA GAATCTATCG TAAGCTTCGA AAAGCTGAAT 
     5281 TGGCAAAACT TCAGCAGACC CTGAATGCAC TTAACAAGAA GGCAGCATTT TATGAAGAGC 
     5341 AAATCAATTA TTATGACACC TACATAAAGA CTTGTTTAGA CAACTTAAAA AGAAAAAATA 
     5401 CTCGGAGATC AATTAAACTA GATGGAAAAG GAGAACCCAA AGGGGCGAAG AGAGCGAAGC 
     5461 CAGTGAAGTA CACTGCAGCA AAGCTGCATG AGAAAGGTGT CCTGCTAGAT ATAGATGATC 
     5521 TTCAAACAAA CCAGTTTAAG AATGTTACAT TTGATATCAT AGCTACTGAA GATGTAGGCA 
     5581 TTTTCGATGT AAGATCAAAA TTCCTTGGTG TTGAGATGGA AAAGGTGCAA CTCAATATTC 
     5641 AGGATTTACT TCAGATGCAA TATGAAGGAG TAGCTGTAAT GAAAATGTTT GATAAGGTTA 
     5701 AAGTGAATGT AAACCTTCTC ATATACCTGC TGAACAAGAA GTTCTATGGA AAGACGCGTA 
     5761 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5821 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5881 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5941 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     6001 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     6061 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     6121 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     6181 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     6241 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     6301 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     6361 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     6421 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     6481 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6541 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6601 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6661 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6721 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6781 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6841 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6901 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6961 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     7021 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     7081 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     7141 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     7201 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     7261 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     7321 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     7381 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     7441 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     7501 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7561 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7621 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7681 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7741 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7801 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7861 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7921 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7981 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     8041 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     8101 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     8161 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     8221 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     8281 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     8341 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     8401 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     8461 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     8521 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8581 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8641 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8701 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8761 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8821 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8881 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8941 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     9001 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     9061 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     9121 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     9181 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     9241 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     9301 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     9361 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     9421 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     9481 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9541 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9601 AATTTT
//