LOCUS       RC223772                7074 bp    DNA     circular  SYN 
DEFINITION  GPR149 (Myc-DDK-tagged)-Human G protein-coupled receptor 149 (GPR149)
ACCESSION   NM_001038705
VERSION     NM_001038705.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3231..3322 
                     /label="Myc-DDK"  
     misc_feature    3352..3937 
                     /label="PolyA signal"  
     misc_feature    3409..3428 
                     /label="Restriction Enzyme"  
     misc_feature    4213..4885 
                     /label="ColE1"  
     misc_feature    5315..6109 
                     /label="Kan/Neo"  
     misc_feature    6144..6446 
                     /label="SV40 ori"  
     misc_feature    6532..7009 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3213..3219 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3213 
                     /label="GPR149"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTTATTT CTCAGTAACT TATCAACAAA TGACTCTAGC CTGTGGAAAG 
     1081 AGAATCATAA TTCTACGGAC CTTTTAAATC CGCCAGGAAC CCTGAATATC TATCTTTTTT 
     1141 GCTTGACATG TCTCATGACT TTTGCAGCCT TGGTGGGCAG CATTTATTCA CTAATTTCCC 
     1201 TGCTGAAAAT GCAGAACAGA ACTGTTGTGT CCATGCTTGT GGCTTCCTGG TCTGTGGATG 
     1261 ATCTCATGAG CGTCCTGTCG GTGACCATCT TCATGTTTTT GCAGTGGCCA AACGAGGTCC 
     1321 CCGGTTACTT CCAATTTCTG TGCACCACCT CTGCCTTAAT GTATTTATGC CAGGGCCTCT 
     1381 CTAGCAACTT GAAGGCGACT CTCCTAGTCT CTTACAACTT TTATACGATG CACAGAGGTG 
     1441 TGGGGAGCCA GACAGCCTCC AGAAGATCGG GCCAGGTGCT CGGCGTGGTG CTGACCGTGT 
     1501 GGGCAGCCAG TCTGCTGCTC TCGGCGCTCC CGCTGTGCGG CTGGGGCGCC TTCGTGCGCA 
     1561 CGCCCTGGGG CTGCCTGGTG GACTGCTCCA GCTCCTACGT ACTATTCCTC TCTATCGTGT 
     1621 ACGCTTTGGC CTTCGGACTC CTCGTGGGCC TCTCAGTCCC ACTCACTCAC CGATTGCTGT 
     1681 GTTCGGAGGA GCCGCCGAGA CTCCACTCCA ACTACCAGGA AATTTCCCGT GGAGCTTCAA 
     1741 TTCCTGGGAC CCCTCCTACT GCGGGGAGAG TGGTTTCCCT GTCCCCAGAG GATGCTCCAG 
     1801 GCCCGAGTCT GCGGCGCTCT GGGGGATGCT CTCCGAGCTC CGACACCGTG TTCGGACCGG 
     1861 GTGCGCCCGC TGCCGCTGGG GCTGAAGCCT GCAGGCGTGA GAACCGGGGG ACTCTCTATG 
     1921 GCACCAGGAG CTTCACCGTG AGCGTAGCGC AGAAGCGCTT CGCTTTGATC CTAGCGCTTA 
     1981 CAAAAGTCGT CCTTTGGCTG CCCATGATGA TGCACATGGT GGTCCAGAAC GTCGTGGGGT 
     2041 TTCAGAGCCT TCCCTTGGAG ACATTCAGCT TTCTACTTAC CCTGCTGGCC ACCACTGTAA 
     2101 CCCCAGTGTT TGTCTTGTCC AAACGCTGGA CCCACTTGCC CTGTGGCTGC ATCATCAACT 
     2161 GCAGGCAGAA CGCATATGCA GTGGCGTCCG ATGGGAAAAA AATCAAGAGA AAAGGCTTTG 
     2221 AATTCAATCT ATCATTCCAA AAAAGTTATG GGATTTATAA AATAGCACAT GAAGATTACT 
     2281 ATGATGATGA TGAAAATTCC ATATTCTATC ACAACCTGAT GAACTCTGAG TGTGAAACTA 
     2341 CAAAAGACCC TCAGAGAGAC AACCGTAACA TCTTCAATGC TATAAAAGTA GAAATCAGCA 
     2401 CCACGCCCTC TCTGGACAGC TCCACACAAA GAGGCATCAA CAAATGCACA AATACTGATA 
     2461 TTACAGAAGC TAAACAGGAT TCCAACAACA AAAAGGATGC GTTTTCTGAC AAAACAGGAG 
     2521 GTGATATTAA CTATGAAGAA ACTACCTTTT CTGAAGGGCC AGAAAGAAGA CTGTCTCATG 
     2581 AAGAGAGTCA GAAACCAGAT CTTTCAGACT GGGAGTGGTG TAGGAGTAAA TCAGAAAGAA 
     2641 CCCCTCGTCA GCGTTCCGGT TATGCCCTTG CCATTCCCTT GTGTGCATTC CAGGGGACTG 
     2701 TGTCTCTCCA TGCACCTACA GGGAAAACCC TATCTCTTTC TACCTATGAG GTAAGCGCAG 
     2761 AAGGGCAAAA AATAACTCCA GCCTCTAAGA AAATAGAAGT CTATCGATCC AAAAGTGTTG 
     2821 GCCATGAACC AAACTCAGAA GATTCTTCAT CCACGTTTGT GGACACCAGT GTGAAAATAC 
     2881 ACTTGGAGGT TCTTGAAATT TGTGATAATG AAGAGGCCTT GGACACTGTG TCAATCATTA 
     2941 GTAACATCAG TCAGTCCTCC ACACAAGTCA GATCTCCATC CCTACGTTAC TCCAGGAAAG 
     3001 AAAACAGATT TGTTTCATGT GACCTAGGGG AAACAGCCTC ATACTCCCTC TTTTTGCCCA 
     3061 CCAGTAATCC TGATGGTGAT ATTAATATCT CCATTCCAGA CACAGTAGAA GCACACAGGC 
     3121 AGAACAGTAA AAGGCAGCAT CAAGAGAGGG ATGGCTACCA GGAGGAAATC CAGTTGTTAA 
     3181 ATAAAGCTTA CAGAAAAAGA GAGGAAGAAA GCAAGGGTAG TACGCGTACG CGGCCGCTCG 
     3241 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3301 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3361 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3421 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3481 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3541 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3601 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3661 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3721 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3781 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3841 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     3901 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     3961 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4021 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4081 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4141 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4201 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4261 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4321 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4381 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4441 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4501 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4561 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4621 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4681 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4741 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4801 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4861 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     4921 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     4981 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5041 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5101 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5161 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5221 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5281 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5341 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5401 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5461 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5521 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5581 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5641 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5701 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5761 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5821 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     5881 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     5941 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6001 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6061 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6121 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6181 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6241 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6301 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6361 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6421 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6481 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6541 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6601 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6661 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6721 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6781 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6841 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     6901 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     6961 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7021 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//