LOCUS       RC223734                8718 bp    DNA     circular  SYN 
DEFINITION  SCAP (Myc-DDK-tagged)-Human SREBF chaperone (SCAP)
ACCESSION   NM_012235
VERSION     NM_012235.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4875..4966 
                     /label="Myc-DDK"  
     misc_feature    4996..5581 
                     /label="PolyA signal"  
     misc_feature    5053..5072 
                     /label="Restriction Enzyme"  
     misc_feature    5857..6529 
                     /label="ColE1"  
     misc_feature    6959..7753 
                     /label="Kan/Neo"  
     misc_feature    7788..8090 
                     /label="SV40 ori"  
     misc_feature    8176..8653 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4857..4863 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4857 
                     /label="SCAP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCCTGACT GAAAGGCTGC GTGAGAAGAT ATCTCGGGCC TTCTACAACC 
     1081 ATGGGCTCCT CTGTGCATCC TATCCCATCC CCATCATCCT CTTCACAGGG TTCTGCATCT 
     1141 TAGCCTGCTG CTACCCACTG CTGAAACTCC CCTTGCCAGG AACAGGACCT GTGGAATTCA 
     1201 CCACCCCTGT GAAGGATTAC TCGCCCCCAC CTGTGGACTC TGACCGCAAA CAAGGAGAGC 
     1261 CTACTGAGCA GCCTGAGTGG TATGTGGGTG CCCCGGTGGC TTATGTCCAG CAGATATTTG 
     1321 TGAAGTCCTC AGTGTTTCCC TGGCACAAGA ACCTCCTGGC AGTAGATGTA TTTCGTTCAC 
     1381 CTTTGTCCCG GGCATTCCAA CTGGTGGAGG AGATCCGGAA CCACGTGCTG AGAGACAGCT 
     1441 CTGGGATCAG GAGCTTGGAG GAGTTGTGTC TGCAAGTGAC CGACCTGCTG CCAGGCCTTA 
     1501 GGAAGCTCAG GAACCTACTC CCTGAGCATG GATGCCTGCT GCTGTCCCCT GGGAACTTCT 
     1561 GGCAGAATGA CTGGGAACGC TTCCATGCTG ATCCTGACAT CATTGGGACC ATCCACCAGC 
     1621 ACGAGCCTAA AACCCTGCAG ACTTCAGCCA CACTCAAAGA CTTGTTATTT GGTGTTCCTG 
     1681 GGAAGTACAG CGGGGTGAGC CTCTACACCA GGAAGAGGAT GGTCTCCTAC ACCATCACCC 
     1741 TGGTCTTCCA GCACTACCAT GCCAAGTTCC TGGGCAGCCT GCGTGCCCGC CTGATGCTTC 
     1801 TGCACCCCAG CCCCAACTGC AGCCTTCGGG CGGAGAGCCT GGTCCACGTG CACTTCAAGG 
     1861 AGGAGATTGG TGTCGCTGAG CTCATCCCCC TTGTGACCAC CTACATCATC TTGTTTGCCT 
     1921 ACATCTACTT CTCCACGCGG AAGATCGACA TGGTCAAGTC CAAGTGGGGG CTGGCCCTGG 
     1981 CTGCCGTGGT CACAGTGCTC AGCTCGCTGC TCATGTCTGT GGGACTCTGC ACACTCTTCG 
     2041 GCCTGACGCC CACCCTCAAT GGCGGCGAGA TTTTCCCCTA CCTTGTGGTG GTTATTGGGT 
     2101 TAGAGAATGT GTTGGTGCTC ACCAAGTCTG TGGTCTCAAC CCCGGTAGAC CTGGAGGTGA 
     2161 AGCTGCGGAT CGCCCAAGGC CTAAGCAGCG AGAGCTGGTC CATCATGAAG AACATGGCCA 
     2221 CGGAGCTGGG CATCATCCTC ATCGGCTACT TCACCCTAGT GCCCGCCATC CAGGAGTTCT 
     2281 GTCTCTTTGC TGTCGTGGGG CTGGTGTCTG ACTTCTTCCT TCAGATGCTG TTTTTCACCA 
     2341 CTGTCCTGTC CATTGACATT CGCCGGATGG AGCTAGCAGA CCTGAACAAG CGACTGCCCC 
     2401 CTGAGGCCTG CCTGCCCTCA GCCAAGCCAG TGGGACAGCC AACGCGCTAC GAGCGGCAGC 
     2461 TGGCTGTGAG GCCGTCCACA CCCCACACCA TCACGTTGCA GCCGTCTTCC TTCCGAAACC 
     2521 TGCGGCTCCC CAAGAGGCTG CGTGTTGTCT ACTTCCTGGC CCGCACCCGC CTGGCACAGC 
     2581 GCCTCATCAT GGCTGGCACC GTTGTCTGGA TTGGCATCCT GGTATACACA GACCCAGCAG 
     2641 GGCTGCGCAA CTACCTCGCT GCCCAGGTGA CGGAACAGAG CCCATTGGGT GAGGGAGCCC 
     2701 TGGCTCCCAT GCCCGTGCCT AGTGGCATGC TGCCCCCCAG CCACCCGGAC CCTGCCTTCT 
     2761 CCATCTTCCC ACCTGATGCC CCTAAGCTAC CTGAGAACCA GACGTCGCCA GGCGAGTCAC 
     2821 CTGAGCGTGG AGGTCCAGCA GAGGTTGTCC ATGACAGCCC AGTCCCAGAG GTAACCTGGG 
     2881 GGCCTGAGGA TGAGGAACTT TGGAGGAAAT TGTCCTTCCG CCACTGGCCG ACGCTCTTCA 
     2941 GCTATTACAA CATCACACTG GCCAAGAGGT ACATCAGCCT GCTGCCCGTC ATCCCAGTCA 
     3001 CGCTCCGCCT GAACCCGAGG GAGGCTCTGG AGGGCCGGCA CCCTCAGGAC GGCCGCAGTG 
     3061 CCTGGCCCCC ACCGGGGCCC ATACCTGCTG GGCACTGGGA AGCAGGACCC AAGGGCCCAG 
     3121 GTGGGGTGCA GGCCCATGGA GACGTCACGC TGTACAAGGT GGCGGCGCTG GGCCTGGCCA 
     3181 CCGGCATCGT CTTGGTGCTG CTGCTGCTCT GCCTCTACCG CGTGCTATGC CCGCGCAACT 
     3241 ACGGGCAGCT GGGTGGTGGG CCCGGGCGGC GGAGGCGCGG GGAGCTGCCC TGCGACGACT 
     3301 ACGGCTATGC GCCACCCGAG ACGGAGATCG TGCCGCTTGT GCTGCGCGGC CACCTCATGG 
     3361 ACATCGAGTG CCTGGCCAGC GACGGCATGC TGCTGGTGAG CTGCTGCCTG GCAGGCCACG 
     3421 TCTGCGTGTG GGACGCGCAG ACCGGGGATT GCCTAACGCG CATTCCGCGC CCAGGCAGGC 
     3481 AGCGCCGGGA CAGTGGCGTG GGCAGCGGGC TTGAGGCTCA GGAGAGCTGG GAACGACTTT 
     3541 CAGATGGTGG GAAGGCTGGT CCAGAGGAGC CTGGGGACAG CCCTCCCCTG AGACACCGCC 
     3601 CCCGGGGCCC TCCGCCGCCT TCCCTCTTCG GGGACCAGCC TGACCTCACC TGCTTAATTG 
     3661 ACACCAACTT TTCAGCGCAG CCTCGGTCCT CACAGCCCAC TCAGCCCGAG CCCCGGCACC 
     3721 GGGCGGTCTG TGGCCGCTCT CGGGACTCCC CAGGCTATGA CTTCAGCTGC CTGGTGCAGC 
     3781 GGGTGTACCA GGAGGAGGGG CTGGCGGCCG TCTGCACACC AGCCCTGCGC CCACCCTCGC 
     3841 CTGGGCCGGT GCTGTCCCAG GCCCCTGAGG ACGAGGGTGG CTCCCCCGAG AAAGGCTCCC 
     3901 CTTCCCTCGC CTGGGCCCCC AGTGCCGAGG GTTCCATCTG GAGCTTGGAG CTGCAGGGCA 
     3961 ACCTCATCGT GGTGGGGCGG AGCAGCGGCC GGCTGGAGGT GTGGGACGCC ATTGAAGGGG 
     4021 TGCTGTGCTG CAGCAGCGAG GAGGTCTCCT CAGGCATTAC CGCTCTGGTG TTCTTGGACA 
     4081 AAAGGATTGT GGCTGCACGG CTCAACGGTT CCCTTGATTT CTTCTCCTTG GAGACCCACA 
     4141 CTGCCCTCAG CCCCCTGCAG TTTAGAGGGA CCCCAGGGCG GGGCAGTTCC CCTGCCTCTC 
     4201 CAGTGTACAG CAGCAGCGAC ACAGTGGCCT GTCACCTGAC CCACACAGTG CCCTGTGCAC 
     4261 ACCAAAAACC CATCACAGCC CTGAAAGCCG CTGCTGGGCG CTTGGTGACT GGGAGCCAAG 
     4321 ACCACACACT GAGAGTGTTC CGTCTGGAGG ACTCGTGCTG CCTCTTCACC CTTCAGGGCC 
     4381 ACTCAGGGGC CATCACGACC GTGTACATTG ACCAGACCAT GGTGCTGGCC AGTGGAGGAC 
     4441 AAGATGGGGC CATCTGCCTG TGGGATGTAC TGACTGGCAG CCGGGTCAGC CATGTGTTTG 
     4501 CTCACCGTGG GGATGTCACC TCCCTTACCT GTACCACCTC CTGTGTCATC AGCAGTGGCC 
     4561 TGGATGACCT CATCAGCATC TGGGACCGCA GCACAGGCAT CAAGTTCTAC TCCATTCAGC 
     4621 AGGACCTGGG CTGTGGTGCA AGCTTGGGTG TCATCTCAGA CAACCTGCTG GTGACTGGCG 
     4681 GCCAGGGCTG TGTCTCCTTT TGGGACCTAA ACTACGGGGA CCTGTTACAG ACAGTCTACC 
     4741 TGGGGAAGAA CAGTGAGGCC CAGCCTGCCC GCCAGATCCT GGTGCTGGAC AACGCTGCCA 
     4801 TTGTCTGCAA CTTTGGCAGT GAGCTCAGCC TGGTGTATGT GCCCTCTGTG CTGGAGAAGC 
     4861 TGGACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4921 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4981 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5041 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5101 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5161 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5221 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5281 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5341 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5401 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5461 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5521 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5581 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5641 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5701 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5761 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5821 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5881 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5941 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6001 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6061 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6121 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6181 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6241 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6301 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6361 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6421 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6481 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6541 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6601 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6661 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6721 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6781 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6841 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6901 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6961 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7021 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7081 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7141 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7201 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7261 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7321 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7381 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7441 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7501 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7561 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7621 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7681 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7741 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7801 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7861 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7921 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7981 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8041 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8101 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8161 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8221 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8281 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8341 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8401 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8461 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8521 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8581 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8641 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8701 AAATTTAACG CGAATTTT
//