LOCUS       RC223715                7836 bp    DNA     circular  SYN 
DEFINITION  AARS2 (Myc-DDK-tagged)-Human alanyl-tRNA synthetase 2, mitochondrial (putative) (AARS2), nuclear gene encoding mitochondrial protein
ACCESSION   NM_020745
VERSION     NM_020745.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3993..4084 
                     /label="Myc-DDK"  
     misc_feature    4114..4699 
                     /label="PolyA signal"  
     misc_feature    4171..4190 
                     /label="Restriction Enzyme"  
     misc_feature    4975..5647 
                     /label="ColE1"  
     misc_feature    6077..6871 
                     /label="Kan/Neo"  
     misc_feature    6906..7208 
                     /label="SV40 ori"  
     misc_feature    7294..7771 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3975..3981 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3975 
                     /label="AARS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCGTCA GTGGCAGCTG CAGCCCGGAG GCTGCGGCGG GCCATTCGAA 
     1081 GGTCGCCCGC ATGGCGGGGC CTCAGCCATC GGCCGCTCTC ATCGGAGCCC CCTGCAGCCA 
     1141 AGGCCTCGGC CGTGAGGGCC GCCTTTCTGA ACTTCTTTCG GGACCGCCAT GGCCACCGGC 
     1201 TGGTGCCCTC CGCTTCCGTG CGGCCCCGCG GCGACCCCAG TTTGCTTTTT GTCAATGCGG 
     1261 GCATGAACCA GTTCAAGCCA ATCTTTCTGG GCACCGTGGA TCCACGAAGC GAGATGGCAG 
     1321 GCTTCCGACG TGTGGCCAAC AGCCAGAAAT GTGTGAGAGC TGGAGGACAC CATAACGACC 
     1381 TGGAAGATGT GGGTCGAGAC CTTTCCCATC ATACCTTCTT TGAAATGCTT GGCAATTGGG 
     1441 CCTTTGGGGG TGAATATTTT AAGGAGGAGG CTTGTAACAT GGCCTGGGAA CTGCTGACTC 
     1501 AGGTCTATGG GATCCCTGAG GAAAGGCTCT GGATCTCCTA CTTTGATGGT GACCCCAAGG 
     1561 CAGGGCTGGA CCCAGACCTG GAGACCAGGG ACATCTGGCT GAGCTTAGGG GTGCCTGCTA 
     1621 GCCGTGTGCT TTCCTTTGGA CCACAAGAGA ACTTCTGGGA GATGGGGGAT ACTGGCCCTT 
     1681 GTGGGCCCTG TACTGAGATC CACTACGACC TTGCTGGTGG GGTGGGAGCC CCCCAGCTGG 
     1741 TAGAGCTTTG GAACCTGGTC TTCATGCAAC ACAACAGAGA GGCAGATGGA AGCCTGCAGC 
     1801 CCCTGCCCCA GCGGCATGTG GACACAGGAA TGGGCCTGGA AAGGCTGGTG GCTGTGCTGC 
     1861 AAGGCAAACA CTCCACCTAT GACACTGACC TCTTTTCCCC GCTGCTCAAC GCCATACAGC 
     1921 AGGGCTGCAG GGCACCCCCT TACTTGGGCC GAGTAGGGGT GGCAGACGAG GGGCGCACAG 
     1981 ACACAGCGTA CCGCGTGGTG GCTGACCACA TCCGCACACT CAGTGTCTGC ATCTCTGATG 
     2041 GCGTCTTCCC TGGGATGTCA GGTCCCCCGC TGGTTCTTCG TCGGATCCTG CGTCGAGCTG 
     2101 TGCGTTTCTC CATGGAGATC TTAAAGGCAC CACCTGGCTT CCTAGGCAGC CTGGTACCTG 
     2161 TAGTGGTGGA GACACTGGGA GATGCTTATC CAGAACTGCA AAGGAACTCA GCCCAGATCG 
     2221 CCAACCTGGT GTCAGAGGAC GAGGCAGCCT TCCTGGCCTC CCTGGAGCGG GGTAGGCGGA 
     2281 TCATTGATCG GACTCTGAGG ACCCTGGGGC CTTCAGATAT GTTCCCTGCT GAAGTGGCCT 
     2341 GGTCCTTGTC ACTGTGTGGA GACCTGGGAC TCCCCTTGGA CATGGTAGAG CTGATGCTGG 
     2401 AGGAGAAAGG GGTCCAGCTA GACTCCGCTG GACTGGAGCG GTTGGCCCAA GAGGAGGCCC 
     2461 AGCACCGGGC ACGGCAGGCT GAGCCAGTTC AGAAGCAGGG ATTGTGGCTT GATGTCCATG 
     2521 CGCTTGGGGA GCTGCAGCGC CAAGGAGTGC CCCCAACTGA CGACAGCCCC AAGTACAACT 
     2581 ACTCCCTGCG ACCCAGCGGA AGTTATGAGT TCGGCACCTG TGAGGCCCAG GTGTTGCAAC 
     2641 TGTATACAGA GGACGGGACA GCAGTGGCCT CCGTGGGGAA AGGCCAGCGC TGTGGCCTCC 
     2701 TCTTGGACAG GACCAACTTC TACGCAGAAC AGGGGGGCCA GGCTTCAGAC CGTGGCTACC 
     2761 TGGTGCGGGC AGGGCAAGAG GACGTGCTGT TCCCAGTAGC CCGGGCCCAG GTCTGTGGAG 
     2821 GTTTCATCCT GCATGAGGCA GTAGCCCCTG AGTGCCTGCG GTTAGGGGAC CAGGTGCAGC 
     2881 TGCATGTGGA TGAGGCCTGG CGTCTAGGCT GCATGGCGAA GCATACGGCC ACCCACCTGC 
     2941 TGAACTGGGC ACTGAGGCAG ACCCTGGGCC CTGGCACAGA GCAGCAGGGC TCCCATCTCA 
     3001 ATCCTGAGCA GCTGCGCTTG GATGTGACCA CCCAGACCCC ATTGACCCCA GAGCAGCTCC 
     3061 GGGCAGTGGA GAACACTGTG CAGGAGGCCG TGGGGCAGGA TGAGGCTGTG TACATGGAGG 
     3121 AGGTGCCCCT GGCGCTCACT GCCCAGGTCC CTGGCCTGCG CTCTCTGGAT GAGGTTTACC 
     3181 CAGACCCTGT GCGGGTGGTA TCAGTGGGGG TGCCCGTGGC CCATGCATTG GACCCAGCCT 
     3241 CCCAAGCCGC ACTGCAGACC TCTGTGGAGC TATGCTGTGG GACGCACCTG TTACGTACTG 
     3301 GGGCTGTAGG GGACCTGGTT ATCATCGGGG ACCGCCAGCT TTCCAAGGGC ACTACCCGCC 
     3361 TGCTGGCCGT CACTGGGGAG CAGGCCCAGC AGGCCCGAGA GCTAGGCCAG AGCCTGGCCC 
     3421 AGGAAGTGAA AGCGGCCACT GAGCGGCTGA GTCTGGGGAG CCGGGATGTG GCGGAGGCAC 
     3481 TGAGGCTGTC CAAGGACATA GGACGACTCA TTGAAGCTGT GGAAACTGCT GTGATGCCCC 
     3541 AGTGGCAGCG GCGGGAGCTG CTGGCCACAG TGAAGATGCT GCAGCGGCGT GCCAACACTG 
     3601 CCATCCGTAA GCTGCAAATG GGACAGGCTG CAAAGAAAAC TCAGGAGCTG CTGGAGCGGC 
     3661 ACTCGAAGGG GCCTCTGATT GTGGACACAG TCTCTGCTGA GTCTCTCTCA GTGCTGGTGA 
     3721 AGGTGGTACG GCAGCTGTGT GAGCAGGCCC CCAGCACGTC TGTGCTCCTA CTCAGCCCCC 
     3781 AGCCCATGGG GAAGGTGCTG TGTGCCTGTC AGGTGGCCCA GGGTGCCATG CCCACCTTCA 
     3841 CAGCAGAGGC CTGGGCACTG GCAGTGTGCA GCCACATGGG GGGCAAGGCG TGGGGCTCGC 
     3901 GAGTGGTGGC CCAAGGCACC GGAAGCACTA CTGACCTGGA AGCTGCCCTC AGTATAGCCC 
     3961 AAACCTATGC CCTCAGCCAG CTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4021 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4081 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4141 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4201 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4261 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4321 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4381 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4441 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4501 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4561 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4621 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4681 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4741 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4801 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4861 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4921 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4981 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5041 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5101 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5161 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5221 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5281 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5341 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5401 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5461 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5521 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5581 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5641 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5701 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5761 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5821 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5881 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5941 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6001 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6061 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6121 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6181 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6241 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6301 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6361 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6421 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6481 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6541 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6601 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6661 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6721 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6781 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6841 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6901 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6961 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7021 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7081 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7141 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7201 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7261 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7321 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7381 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7441 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7501 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7561 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7621 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7681 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7741 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7801 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//