LOCUS       RC223684                8655 bp    DNA     circular  SYN 
DEFINITION  ATP7B (Myc-DDK-tagged)-Human ATPase, Cu++ transporting, beta polypeptide (ATP7B), transcript variant 2
ACCESSION   NM_001005918
VERSION     NM_001005918.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4812..4903 
                     /label="Myc-DDK"  
     misc_feature    4933..5518 
                     /label="PolyA signal"  
     misc_feature    4990..5009 
                     /label="Restriction Enzyme"  
     misc_feature    5794..6466 
                     /label="ColE1"  
     misc_feature    6896..7690 
                     /label="Kan/Neo"  
     misc_feature    7725..8027 
                     /label="SV40 ori"  
     misc_feature    8113..8590 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4794..4800 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4794 
                     /label="ATP7B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGAGCAG GAGAGACAGA TCACAGCCAG AGAAGGGGCC AGTCGGAAAA 
     1081 TCTTATCTAA GCTTTCTTTG CCTACCCGTG CCTGGGAACC AGCAATGAAG AAGAGTTTTG 
     1141 CTTTTGACAA TGTTGGCTAT GAAGGTGGTC TGGATGGCCT GGGCCCTTCT TCTCAGGTGG 
     1201 CCACCAGCAC AGTCAGGATC TTGGGCATGA CTTGCCAGTC ATGTGTGAAG TCCATTGAGG 
     1261 ACAGGATTTC CAATTTGAAA GGCATCATCA GCATGAAGGT TTCCCTGGAA CAAGGCAGTG 
     1321 CCACTGTGAA ATATGTGCCA TCGGTTGTGT GCCTGCAACA GGTTTGCCAT CAAATTGGGG 
     1381 ACATGGGCTT CGAGGCCAGC ATTGCAGAAG GAAAGGCAGC CTCCTGGCCC TCAAGGTCCT 
     1441 TGCCTGCCCA GGAGGCTGTG GTCAAGCTCC GGGTGGAGGG CATGACCTGC CAGTCCTGTG 
     1501 TCAGCTCCAT TGAAGGCAAG GTCCGGAAAC TGCAAGGAGT AGTGAGAGTC AAAGTCTCAC 
     1561 TCAGCAACCA AGAGGCCGTC ATCACTTATC AGCCTTATCT CATTCAGCCC GAAGACCTCA 
     1621 GGGACCATGT AAATGACATG GGATTTGAAG CTGCCATCAA GAGCAAAGTG GCTCCCTTAA 
     1681 GCCTGGGACC AATTGATATT GAGCGGTTAC AAAGCACTAA CCCAAAGAGA CCTTTATCTT 
     1741 CTGCTAACCA GAATTTTAAT AATTCTGAGA CCTTGGGGCA CCAAGGAAGC CATGTGGTCA 
     1801 CCCTCCAACT GAGAATAGAT GGAATGCATT GTAAGTCTTG CGTCTTGAAT ATTGAAGAAA 
     1861 ATATTGGCCA GCTCCTAGGG GTTCAAAGTA TTCAAGTGTC CTTGGAGAAC AAAACTGCCC 
     1921 AAGTAAAGTA TGACCCTTCT TGTACCAGCC CAGTGGCTCT GCAGAGGGCT ATCGAGGCAC 
     1981 TTCCACCTGG GAATTTTAAA GTTTCTCTTC CTGATGGAGC CGAAGGGAGT GGGACAGATC 
     2041 ACAGGTCTTC CAGTTCTCAT TCCCCTGGCT CCCCACCGAG AAACCAGGTC CAGGGCACAT 
     2101 GCAGTACCAC TCTGATTGCC ATTGCCGGCA TGACCTGTGC ATCCTGTGTC CATTCCATTG 
     2161 AAGGCATGAT CTCCCAACTG GAAGGGGTGC AGCAAATATC GGTGTCTTTG GCCGAAGGGA 
     2221 CTGCAACAGT TCTTTATAAT CCCTCTGTAA TTAGCCCAGA AGAACTCAGA GCTGCTATAG 
     2281 AAGACATGGG ATTTGAGGCT TCAGTCGTTT CTGAAAGCTG TTCTACTAAC CCTCTTGGAA 
     2341 ACCACAGTGC TGGGAATTCC ATGGTGCAAA CTACAGATGG TACACCTACA TCTGTGCAGG 
     2401 AAGTGGCTCC CCACACTGGG AGGCTCCCTG CAAACCATGC CCCGGACATC TTGGCAAAGT 
     2461 CCCCACAATC AACCAGAGCA GTGGCACCGC AGAAGTGCTT CTTACAGATC AAAGGCATGA 
     2521 CCTGTGCATC CTGTGTGTCT AACATAGAAA GGAATCTGCA GAAAGAAGCT GGTGTTCTCT 
     2581 CCGTGTTGGT TGCCTTGATG GCAGGAAAGG CAGAGATCAA GTATGACCCA GAGGTCATCC 
     2641 AGCCCCTCGA GATAGCTCAG TTCATCCAGG ACCTGGGTTT TGAGGCAGCA GTCATGGAGG 
     2701 ACTACGCAGG CTCCGATGGC AACATTGAGC TGACAATCAC AGGGATGACC TGCGCGTCCT 
     2761 GTGTCCACAA CATAGAGTCC AAACTCACGA GGACAAATGG CATCACTTAT GCCTCCGTTG 
     2821 CCCTTGCCAC CAGCAAAGCC CTTGTTAAGT TTGACCCGGA AATTATCGGT CCACGGGATA 
     2881 TTATCAAAAT TATTGAGAGC AAAACCTCAG AAGCCCTGGC TAAACTCATG TCTCTCCAAG 
     2941 CCACAGAAGC CACCGTTGTG ACCCTTGGTG AGGACAATTT AATCATCAGG GAGGAGCAAG 
     3001 TCCCCATGGA GCTGGTGCAG CGGGGCGATA TCGTCAAGGT GGTCCCTGGG GGAAAGTTTC 
     3061 CAGTGGATGG GAAAGTCCTG GAAGGCAATA CCATGGCTGA TGAGTCCCTC ATCACAGGAG 
     3121 AAGCCATGCC AGTCACTAAG AAACCCGGAA GCACTGTAAT TGCGGGGTCT ATAAATGCAC 
     3181 ATGGCTCTGT GCTCATTAAA GCTACCCACG TGGGCAATGA CACCACTTTG GCTCAGATTG 
     3241 TGAAACTGGT GGAAGAGGCT CAGATGTCAA AGAACCCCAA CAAGCACATC TCCCAGACAG 
     3301 AGGTGATCAT CCGGTTTGCT TTCCAGACGT CCATCACGGT GCTGTGCATT GCCTGCCCCT 
     3361 GCTCCCTGGG GCTGGCCACG CCCACGGCTG TCATGGTGGG CACCGGGGTG GCCGCGCAGA 
     3421 ACGGCATCCT CATCAAGGGA GGCAAGCCCC TGGAGATGGC GCACAAGATA AAGACTGTGA 
     3481 TGTTTGACAA GACTGGCACC ATTACCCATG GCGTCCCCAG GGTCATGCGG GTGCTCCTGC 
     3541 TGGGGGATGT GGCCACACTG CCCCTCAGGA AGGTTCTGGC TGTGGTGGGG ACTGCGGAGG 
     3601 CCAGCAGTGA ACACCCCTTG GGCGTGGCAG TCACCAAATA CTGTAAAGAG GAACTTGGAA 
     3661 CAGAGACCTT GGGATACTGC ACGGACTTCC AGGCAGTGCC AGGCTGTGGA ATTGGGTGCA 
     3721 AAGTCAGCAA CGTGGAAGGC ATCCTGGCCC ACAGTGAGCG CCCTTTGAGT GCACCGGCCA 
     3781 GTCACCTGAA TGAGGCTGGC AGCCTTCCCG CAGAAAAAGA TGCAGTCCCC CAGACCTTCT 
     3841 CTGTGCTGAT TGGAAACCGT GAGTGGCTGA GGCGCAACGG TTTAACCATT TCTAGCGATG 
     3901 TCAGTGACGC TATGACAGAC CACGAGATGA AAGGACAGAC AGCCATCCTG GTGGCTATTG 
     3961 ACGGTGTGCT CTGTGGGATG ATCGCAATCG CAGACGCTGT CAAGCAGGAG GCTGCCCTGG 
     4021 CTGTGCACAC GCTGCAGAGC ATGGGTGTGG ACGTGGTTCT GATCACGGGG GACAACCGGA 
     4081 AGACAGCCAG AGCTATTGCC ACCCAGGTTG GCATCAACAA AGTCTTTGCA GAGGTGCTGC 
     4141 CTTCGCACAA GGTGGCCAAG GTCCAGGAGC TCCAGAATAA AGGGAAGAAA GTCGCCATGG 
     4201 TGGGGGATGG GGTCAATGAC TCCCCGGCCT TGGCCCAGGC AGACATGGGT GTGGCCATTG 
     4261 GCACCGGCAC GGATGTGGCC ATCGAGGCAG CCGACGTCGT CCTTATCAGA AATGATTTGC 
     4321 TGGATGTGGT GGCTAGCATT CACCTTTCCA AGAGGACTGT CCGAAGGATA CGCATCAACC 
     4381 TGGTCCTGGC ACTGATTTAT AACCTGGTTG GGATACCCAT TGCAGCAGGT GTCTTCATGC 
     4441 CCATCGGCAT TGTGCTGCAG CCCTGGATGG GCTCAGCGGC CATGGCAGCC TCCTCTGTGT 
     4501 CTGTGGTGCT CTCATCCCTG CAGCTCAAGT GCTATAAGAA GCCTGACCTG GAGAGGTATG 
     4561 AGGCACAGGC GCATGGCCAC ATGAAGCCCC TGACGGCATC CCAGGTCAGT GTGCACATAG 
     4621 GCATGGATGA CAGGTGGCGG GACTCCCCCA GGGCCACACC ATGGGACCAG GTCAGCTATG 
     4681 TCAGCCAGGT GTCGCTGTCC TCCCTGACGT CCGACAAGCC ATCTCGGCAC AGCGCTGCAG 
     4741 CAGACGATGA TGGGGACAAG TGGTCTCTGC TCCTGAATGG CAGGGATGAG GAGCAGTACA 
     4801 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4861 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4921 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4981 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5041 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5101 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5161 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5221 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5281 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5341 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5401 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5461 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5521 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5581 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5641 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5701 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5761 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5821 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5881 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5941 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6001 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6061 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6121 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6181 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6241 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6301 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6361 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6421 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6481 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6541 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6601 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6661 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6721 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6781 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6841 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6901 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6961 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7021 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7081 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7141 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7201 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7261 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7321 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7381 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7441 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7501 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7561 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7621 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7681 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7741 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7801 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7861 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7921 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7981 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8041 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8101 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8161 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8221 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8281 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8341 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8401 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8461 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8521 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8581 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8641 TTTAACGCGA ATTTT
//