LOCUS       RC223673L1                9111 bp    DNA     circular  SYN 
DEFINITION  GRM4 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 4 (GRM4)
ACCESSION   NM_000841
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7402..7582 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8310..8969 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7741..8309 
                     /label="ColE1"  
     misc_feature    6277..6297 
                     /label="LR50 primer"  
     misc_feature    6074..6145 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6286..6877 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6056..6062 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6056 
                     /label="GRM4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCTGGGAAG AGAGGCTTGG GCTGGTGGTG 
     3361 GGCCCGGCTG CCCCTTTGCC TGCTCCTCAG CCTTTACGGC CCCTGGATGC CTTCCTCCCT 
     3421 GGGAAAGCCC AAAGGCCACC CTCACATGAA TTCCATCCGC ATAGATGGGG ACATCACACT 
     3481 GGGAGGCCTG TTCCCGGTGC ATGGCCGGGG CTCAGAGGGC AAGCCCTGTG GAGAACTTAA 
     3541 GAAGGAAAAG GGCATCCACC GGCTGGAGGC CATGCTGTTC GCCCTGGATC GCATCAACAA 
     3601 CGACCCGGAC CTGCTGCCTA ACATCACGCT GGGCGCCCGC ATTCTGGACA CCTGCTCCAG 
     3661 GGACACCCAT GCCCTCGAGC AGTCGCTGAC CTTTGTGCAG GCGCTCATCG AGAAGGATGG 
     3721 CACAGAGGTC CGCTGTGGCA GTGGCGGCCC ACCCATCATC ACCAAGCCTG AACGTGTGGT 
     3781 GGGTGTCATC GGTGCTTCAG GGAGCTCGGT CTCCATCATG GTGGCCAACA TCCTTCGCCT 
     3841 CTTCAAGATA CCCCAGATCA GCTACGCCTC CACAGCGCCA GACCTGAGTG ACAACAGCCG 
     3901 CTACGATTTC TTCTCCCGCG TGGTGCCCTC GGACACGTAC CAGGCCCAGG CCATGGTGGA 
     3961 CATCGTCCGT GCCCTCAAGT GGAACTATGT GTCCACAGTG GCCTCGGAGG GCAGCTATGG 
     4021 TGAGAGCGGT GTGGAGGCCT TCATCCAGAA GTCCCGTGAG GACGGGGGCG TGTGCATCGC 
     4081 CCAGTCGGTG AAGATACCAC GGGAGCCCAA GGCAGGCGAG TTCGACAAGA TCATCCGCCG 
     4141 CCTCCTGGAG ACTTCGAACG CCAGGGCAGT CATCATCTTT GCCAACGAGG ATGACATCAG 
     4201 GCGTGTGCTG GAGGCAGCAC GAAGGGCCAA CCAGACAGGC CATTTCTTCT GGATGGGCTC 
     4261 TGACAGCTGG GGCTCCAAGA TTGCACCTGT GCTGCACCTG GAGGAGGTGG CTGAGGGTGC 
     4321 TGTCACGATC CTCCCCAAGA GGATGTCCGT ACGAGGCTTC GACCGCTACT TCTCCAGCCG 
     4381 CACGCTGGAC AACAACCGGC GCAACATCTG GTTTGCCGAG TTCTGGGAGG ACAACTTCCA 
     4441 CTGCAAGCTG AGCCGCCACG CCCTCAAGAA GGGCAGCCAC GTCAAGAAGT GCACCAACCG 
     4501 TGAGCGAATT GGGCAGGATT CAGCTTATGA GCAGGAGGGG AAGGTGCAGT TTGTGATCGA 
     4561 TGCCGTGTAC GCCATGGGCC ACGCGCTGCA CGCCATGCAC CGTGACCTGT GTCCCGGCCG 
     4621 CGTGGGGCTC TGCCCGCGCA TGGACCCTGT AGATGGCACC CAGCTGCTTA AGTACATCCG 
     4681 AAACGTCAAC TTCTCAGGCA TCGCAGGGAA CCCTGTGACC TTCAATGAGA ATGGAGATGC 
     4741 GCCTGGGCGC TATGACATCT ACCAATACCA GCTGCGCAAC GATTCTGCCG AGTACAAGGT 
     4801 CATTGGCTCC TGGACTGACC ACCTGCACCT TAGAATAGAG CGGATGCACT GGCCGGGGAG 
     4861 CGGGCAGCAG CTGCCCCGCT CCATCTGCAG CCTGCCCTGC CAACCGGGTG AGCGGAAGAA 
     4921 GACAGTGAAG GGCATGCCTT GCTGCTGGCA CTGCGAGCCT TGCACAGGGT ACCAGTACCA 
     4981 GGTGGACCGC TACACCTGTA AGACGTGTCC CTATGACATG CGGCCCACAG AGAACCGCAC 
     5041 GGGCTGCCGG CCCATCCCCA TCATCAAGCT TGAGTGGGGC TCGCCCTGGG CCGTGCTGCC 
     5101 CCTCTTCCTG GCCGTGGTGG GCATCGCTGC CACGTTGTTC GTGGTGATCA CCTTTGTGCG 
     5161 CTACAACGAC ACGCCCATCG TCAAGGCCTC GGGCCGTGAA CTGAGCTACG TGCTGCTGGC 
     5221 AGGCATCTTC CTGTGCTATG CCACCACCTT CCTCATGATC GCTGAGCCCG ACCTTGGCAC 
     5281 CTGCTCGCTG CGCCGAATCT TCCTGGGACT AGGGATGAGC ATCAGCTATG CAGCCCTGCT 
     5341 CACCAAGACC AACCGCATCT ACCGCATCTT CGAGCAGGGC AAGCGCTCGG TCAGTGCCCC 
     5401 ACGCTTCATC AGCCCCGCCT CACAGCTGGC CATCACCTTC AGCCTCATCT CGCTGCAGCT 
     5461 GCTGGGCATC TGTGTGTGGT TTGTGGTGGA CCCCTCCCAC TCGGTGGTGG ACTTCCAGGA 
     5521 CCAGCGGACA CTCGACCCCC GCTTCGCCAG GGGTGTGCTC AAGTGTGACA TCTCGGACCT 
     5581 GTCGCTCATC TGCCTGCTGG GCTACAGCAT GCTGCTCATG GTCACGTGCA CCGTGTATGC 
     5641 CATCAAGACA CGCGGCGTGC CCGAGACCTT CAATGAGGCC AAGCCCATTG GCTTCACCAT 
     5701 GTACACCACT TGCATCGTCT GGCTGGCCTT CATCCCCATC TTCTTTGGCA CCTCGCAGTC 
     5761 GGCCGACAAG CTGTACATCC AGACGACGAC GCTGACGGTC TCGGTGAGTC TGAGCGCCTC 
     5821 GGTGTCCCTG GGAATGCTCT ACATGCCCAA AGTCTACATC ATCCTCTTCC ACCCGGAGCA 
     5881 GAATGTGCCC AAGCGCAAGC GCAGCCTCAA AGCCGTCGTT ACGGCGGCCA CCATGTCCAA 
     5941 CAAGTTCACG CAGAAGGGCA ACTTCCGGCC CAACGGAGAG GCCAAGTCTG AGCTCTGCGA 
     6001 GAACCTTGAG GCCCCAGCGC TGGCCACCAA ACAGACTTAC GTCACTTACA CCAACCATGC 
     6061 AATCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     6121 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA 
     6181 CAAGTAGGAT TCGTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA 
     6241 CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA TCAAGCTTAT CGATAATCAA 
     6301 CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT 
     6361 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT 
     6421 TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC 
     6481 GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG 
     6541 GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC 
     6601 ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC 
     6661 ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT 
     6721 GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA 
     6781 GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT 
     6841 CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGACCT 
     6901 CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC 
     6961 TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG TCACACCTCA 
     7021 GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA 
     7081 AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC TTGATCTGTG 
     7141 GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG GGCCAGGGAT 
     7201 CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT 
     7261 AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC TGCATGGGAT 
     7321 GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA 
     7381 CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC 
     7441 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG 
     7501 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG 
     7561 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA GCAAAAGGCC AGCAAAAGGC 
     7621 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     7681 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     7741 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     7801 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     7861 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     7921 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     7981 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     8041 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     8101 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     8161 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     8221 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     8281 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGATT ACGCCCCGCC CTGCCACTCA 
     8341 TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA 
     8401 TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC 
     8461 ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG 
     8521 AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA 
     8581 TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG 
     8641 AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG 
     8701 GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG 
     8761 AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG 
     8821 TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA 
     8881 TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT 
     8941 ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATAC TCTTCCTTTT TCAATATTAT 
     9001 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     9061 AATAAACAAA TAGGGGTCCC GCGCACATTT CCCCGAAAAG TGCCACCTGA C
//