LOCUS       RC223650                8271 bp    DNA     circular  SYN 
DEFINITION  LATS1 (Myc-DDK-tagged)-Human LATS, large tumor suppressor, homolog 1 (Drosophila) (LATS1)
ACCESSION   NM_004690
VERSION     NM_004690.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4428..4519 
                     /label="Myc-DDK"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    4606..4625 
                     /label="Restriction Enzyme"  
     misc_feature    5410..6082 
                     /label="ColE1"  
     misc_feature    6512..7306 
                     /label="Kan/Neo"  
     misc_feature    7341..7643 
                     /label="SV40 ori"  
     misc_feature    7729..8206 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4410..4416 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4410 
                     /label="LATS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAGGAGT GAAAAGCCAG AAGGATATAG ACAAATGAGG CCTAAGACCT 
     1081 TTCCTGCCAG TAACTATACT GTCAGTAGCC GGCAAATGTT ACAAGAAATT CGGGAATCCC 
     1141 TTAGGAATTT ATCTAAACCA TCTGATGCTG CTAAGGCTGA GCATAACATG AGTAAAATGT 
     1201 CAACCGAAGA TCCTCGACAA GTCAGAAATC CACCCAAATT TGGGACGCAT CATAAAGCCT 
     1261 TGCAGGAAAT TCGAAACTCT CTGCTTCCAT TTGCAAATGA AACAAATTCT TCTCGGAGTA 
     1321 CTTCAGAAGT TAATCCACAA ATGCTTCAAG ACTTGCAAGC TGCTGGATTT GATGAGGATA 
     1381 TGGTTATACA AGCTCTTCAG AAAACTAACA ACAGAAGTAT AGAAGCAGCA ATTGAATTCA 
     1441 TTAGTAAAAT GAGTTACCAA GATCCTCGAC GAGAGCAGAT GGCTGCAGCA GCTGCCAGAC 
     1501 CTATTAATGC CAGCATGAAA CCAGGGAATG TGCAGCAATC AGTTAACCGC AAACAGAGCT 
     1561 GGAAAGGTTC TAAAGAATCC TTAGTTCCTC AGAGGCATGG CCCGCCACTA GGAGAAAGTG 
     1621 TGGCCTATCA TTCTGAGAGT CCCAACTCAC AGACAGATGT AGGAAGACCT TTGTCTGGAT 
     1681 CTGGTATATC AGCATTTGTT CAAGCTCACC CTAGCAACGG ACAGAGAGTG AACCCCCCAC 
     1741 CACCACCTCA AGTAAGGAGT GTTACTCCTC CACCACCTCC AAGAGGCCAG ACTCCCCCTC 
     1801 CAAGAGGTAC AACTCCACCT CCCCCTTCAT GGGAACCAAA CTCTCAAACA AAGCGCTATT 
     1861 CTGGAAACAT GGAATACGTA ATCTCCCGAA TCTCTCCTGT CCCACCTGGG GCATGGCAAG 
     1921 AGGGCTATCC TCCACCACCT CTCAACACTT CCCCCATGAA TCCTCCTAAT CAAGGACAGA 
     1981 GAGGCATTAG TTCTGTTCCT GTTGGCAGAC AACCAATCAT CATGCAGAGT TCTAGCAAAT 
     2041 TTAACTTTCC ATCAGGGAGA CCTGGAATGC AGAATGGTAC TGGACAAACT GATTTCATGA 
     2101 TACACCAAAA TGTTGTCCCT GCTGGCACTG TGAATCGGCA GCCACCACCT CCATATCCTC 
     2161 TGACAGCAGC TAATGGACAA AGCCCTTCTG CTTTACAAAC AGGGGGATCT GCTGCTCCTT 
     2221 CGTCATATAC AAATGGAAGT ATTCCTCAGT CTATGATGGT GCCAAACAGA AATAGTCATA 
     2281 ACATGGAACT ATATAACATT AGTGTACCTG GACTGCAAAC AAATTGGCCT CAGTCATCTT 
     2341 CTGCTCCAGC CCAGTCATCC CCGAGCAGTG GGCATGAAAT CCCTACATGG CAACCTAACA 
     2401 TACCAGTGAG GTCAAATTCT TTTAATAACC CATTAGGAAA TAGAGCAAGT CACTCTGCTA 
     2461 ATTCTCAGCC TTCCGCTACA ACAGTCACTG CAATTACACC AGCTCCTATT CAACAGCCTG 
     2521 TGAAAAGTAT GCGTGTATTA AAACCAGAGC TACAGACTGC TTTAGCACCT ACACACCCTT 
     2581 CTTGGATACC ACAGCCAATT CAAACTGTTC AACCCAGTCC TTTTCCTGAG GGAACCGCTT 
     2641 CAAATGTGAC TGTGATGCCA CCTGTTGCTG AAGCTCCAAA CTATCAAGGA CCACCACCAC 
     2701 CCTACCCAAA ACATCTGCTG CACCAAAACC CATCTGTTCC TCCATACGAG TCAATCAGTA 
     2761 AGCCTAGCAA AGAGGATCAG CCAAGCTTGC CCAAGGAAGA TGAGAGTGAA AAGAGTTATG 
     2821 AAAATGTTGA TAGTGGGGAT AAAGAAAAGA AACAGATTAC AACTTCACCT ATTACTGTTA 
     2881 GGAAAAACAA GAAAGATGAA GAGCGAAGGG AATCTCGTAT TCAAAGTTAT TCTCCTCAAG 
     2941 CATTTAAATT CTTTATGGAG CAACATGTAG AAAATGTACT CAAATCTCAT CAGCAGCGTC 
     3001 TACATCGTAA AAAACAATTA GAGAATGAAA TGATGCGGGT TGGATTATCT CAAGATGCCC 
     3061 AGGATCAAAT GAGAAAGATG CTTTGCCAAA AAGAATCTAA TTACATCCGT CTTAAAAGGG 
     3121 CTAAAATGGA CAAGTCTATG TTTGTGAAGA TAAAGACACT AGGAATAGGA GCATTTGGTG 
     3181 AAGTCTGTCT AGCAAGAAAA GTAGATACTA AGGCTTTGTA TGCAACAAAA ACTCTTCGAA 
     3241 AGAAAGATGT TCTTCTTCGA AATCAAGTCG CTCATGTTAA GGCTGAGAGA GATATCCTGG 
     3301 CTGAAGCTGA CAATGAATGG GTAGTTCGTC TATATTATTC ATTCCAAGAT AAGGACAATT 
     3361 TATACTTTGT AATGGACTAC ATTCCTGGGG GTGATATGAT GAGCCTATTA ATTAGAATGG 
     3421 GCATCTTTCC AGAAAGTCTG GCACGATTCT ACATAGCAGA ACTTACCTGT GCAGTTGAAA 
     3481 GTGTTCATAA AATGGGTTTT ATTCATAGAG ATATTAAACC TGATAATATT TTGATTGATC 
     3541 GTGATGGTCA TATTAAATTG ACTGACTTTG GCCTCTGCAC TGGCTTCAGA TGGACACACG 
     3601 ATTCTAAGTA CTATCAGAGT GGTGACCATC CACGGCAAGA TAGCATGGAT TTCAGTAATG 
     3661 AATGGGGGGA TCCCTCAAGC TGTCGATGTG GAGACAGACT GAAGCCATTA GAGCGGAGAG 
     3721 CTGCACGCCA GCACCAGCGA TGTCTAGCAC ATTCTTTGGT TGGGACTCCC AATTATATTG 
     3781 CACCTGAAGT GTTGCTACGA ACAGGATACA CACAGTTGTG TGATTGGTGG AGTGTTGGTG 
     3841 TTATTCTTTT TGAAATGTTG GTGGGACAAC CTCCTTTCTT GGCACAAACA CCATTAGAAA 
     3901 CACAAATGAA GGTTATCAAC TGGCAAACAT CTCTTCACAT TCCACCACAA GCTAAACTCA 
     3961 GTCCTGAAGC TTCTGATCTT ATTATTAAAC TTTGCCGAGG ACCCGAAGAT CGCTTAGGCA 
     4021 AGAATGGTGC TGATGAAATA AAAGCTCATC CATTTTTTAA AACAATTGAT TTCTCCAGTG 
     4081 ACCTGAGACA GCAGTCTGCT TCATACATTC CTAAAATCAC ACACCCAACA GATACATCAA 
     4141 ATTTTGATCC TGTTGATCCT GATAAATTAT GGAGTGATGA TAACGAGGAA GAAAATGTAA 
     4201 ATGACACTCT CAATGGATGG TATAAAAATG GAAAGCATCC TGAACATGCA TTCTATGAAT 
     4261 TTACCTTCCG AAGGTTTTTT GATGACAATG GCTACCCATA TAATTATCCG AAGCCTATTG 
     4321 AATATGAATA CATTAATTCA CAAGGCTCAG AGCAGCAGTC GGATGAAGAT GATCAAAACA 
     4381 CAGGCTCAGA GATTAAAAAT CGCGATCTAG TATATGTTAC GCGTACGCGG CCGCTCGAGC 
     4441 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4501 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6061 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6121 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6181 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6241 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6301 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6361 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6421 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6481 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6541 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6601 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6661 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6721 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6781 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6841 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6901 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6961 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7021 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7081 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7141 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7201 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7261 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7321 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7381 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7441 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7501 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7681 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7861 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7921 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7981 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8041 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8101 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8161 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8221 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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