LOCUS       RC223585                7491 bp    DNA     circular  SYN 
DEFINITION  DNM2 (Myc-DDK-tagged)-Human dynamin 2 (DNM2), transcript variant 1
ACCESSION   NM_001005360
VERSION     NM_001005360.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3648..3739 
                     /label="Myc-DDK"  
     misc_feature    3769..4354 
                     /label="PolyA signal"  
     misc_feature    3826..3845 
                     /label="Restriction Enzyme"  
     misc_feature    4630..5302 
                     /label="ColE1"  
     misc_feature    5732..6526 
                     /label="Kan/Neo"  
     misc_feature    6561..6863 
                     /label="SV40 ori"  
     misc_feature    6949..7426 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3630..3636 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3630 
                     /label="DNM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAACCGC GGGATGGAAG AGCTGATCCC GCTGGTCAAC AAACTGCAGG 
     1081 ACGCCTTCAG CTCCATCGGC CAGAGCTGCC ACCTGGACCT GCCGCAGATC GCTGTAGTGG 
     1141 GCGGCCAGAG CGCCGGCAAG AGCTCGGTGC TGGAGAACTT CGTGGGCCGG GACTTCCTTC 
     1201 CCCGCGGTTC AGGAATCGTC ACCCGGCGGC CTCTCATTCT GCAGCTCATC TTCTCAAAAA 
     1261 CAGAACATGC CGAGTTTTTG CACTGCAAGT CCAAAAAGTT TACAGACTTT GATGAAGTCC 
     1321 GGCAGGAGAT TGAAGCAGAG ACCGACAGGG TCACGGGGAC CAACAAAGGC ATCTCCCCAG 
     1381 TGCCCATCAA CCTTCGAGTC TACTCGCCAC ACGTGTTGAA CTTGACCCTC ATCGACCTCC 
     1441 CGGGTATCAC CAAGGTGCCT GTGGGCGACC AGCCTCCAGA CATCGAGTAC CAGATCAAGG 
     1501 ACATGATCCT GCAGTTCATC AGCCGGGAGA GCAGCCTCAT TCTGGCTGTC ACGCCCGCCA 
     1561 ACATGGACCT GGCCAACTCC GACGCCCTCA AGCTGGCCAA GGAAGTCGAT CCCCAAGGCC 
     1621 TACGGACCAT CGGTGTCATC ACCAAGCTTG ACCTGATGGA CGAGGGCACC GACGCCAGGG 
     1681 ACGTCTTGGA GAACAAGTTG CTCCCGTTGA GAAGAGGCTA CATTGGCGTG GTGAACCGCA 
     1741 GCCAGAAGGA TATTGAGGGC AAGAAGGACA TCCGTGCAGC ACTGGCAGCT GAGAGGAAGT 
     1801 TCTTCCTCTC CCACCCGGCC TACCGGCACA TGGCCGACCG CATGGGCACG CCACATCTGC 
     1861 AGAAGACGCT GAATCAGCAA CTGACCAACC ACATCCGGGA GTCGCTGCCG GCCCTACGTA 
     1921 GCAAACTACA GAGCCAGCTG CTGTCCCTGG AGAAGGAGGT GGAGGAGTAC AAGAACTTTC 
     1981 GGCCCGACGA CCCCACCCGC AAAACCAAAG CCCTGCTGCA GATGGTCCAG CAGTTTGGGG 
     2041 TGGATTTTGA GAAGAGGATC GAGGGCTCAG GAGATCAGGT GGACACTCTG GAGCTCTCCG 
     2101 GGGGCGCCCG AATCAATCGC ATCTTCCACG AGCGGTTCCC ATTTGAGCTG GTGAAGATGG 
     2161 AGTTTGACGA GAAGGACTTA CGACGGGAGA TCAGCTATGC CATTAAGAAC ATCCATGGAG 
     2221 TCAGGACCGG GCTTTTCACC CCGGACTTGG CATTCGAGGC CATTGTGAAA AAGCAGGTCG 
     2281 TCAAGCTGAA AGAGCCCTGT CTGAAATGTG TCGACCTGGT TATCCAGGAG CTAATCAATA 
     2341 CAGTTAGGCA GTGTACCAGT AAGCTCAGTT CCTACCCCCG GTTGCGAGAG GAGACAGAGC 
     2401 GAATCGTCAC CACTTACATC CGGGAACGGG AGGGGAGAAC GAAGGACCAG ATTCTTCTGC 
     2461 TGATCGACAT TGAGCAGTCC TACATCAACA CGAACCATGA GGACTTCATC GGGTTTGCCA 
     2521 ATGCCCAGCA GAGGAGCACG CAGCTGAACA AGAAGAGAGC CATCCCCAAT CAGGGGGAGA 
     2581 TCCTGGTGAT CCGCAGGGGC TGGCTGACCA TCAACAACAT CAGCCTGATG AAAGGCGGCT 
     2641 CCAAGGAGTA CTGGTTTGTG CTGACTGCCG AGTCACTGTC CTGGTACAAG GATGAGGAGG 
     2701 AGAAAGAGAA GAAGTACATG CTGCCTCTGG ACAACCTCAA GATCCGTGAT GTGGAGAAGG 
     2761 GCTTCATGTC CAACAAGCAC GTCTTCGCCA TCTTCAACAC GGAGCAGAGA AACGTCTACA 
     2821 AGGACCTGCG GCAGATCGAG CTGGCCTGTG ACTCCCAGGA AGACGTGGAC AGCTGGAAGG 
     2881 CCTCGTTCCT CCGAGCTGGC GTCTACCCCG AGAAGGACCA GGCAGAAAAC GAGGATGGGG 
     2941 CCCAGGAGAA CACCTTCTCC ATGGACCCCC AACTGGAGCG GCAGGTGGAG ACCATTCGCA 
     3001 ACCTGGTGGA CTCATACGTG GCCATCATCA ACAAGTCCAT CCGCGACCTC ATGCCAAAGA 
     3061 CCATCATGCA CCTCATGATC AACAATACGA AGGCCTTCAT CCACCACGAG CTGCTGGCCT 
     3121 ACCTATACTC CTCGGCAGAC CAGAGCAGCC TCATGGAGGA GTCGGCCGAC CAGGCACAGC 
     3181 GGCGGGACGA CATGCTGCGC ATGTACCATG CCCTCAAGGA GGCGCTCAAC ATCATCGGTG 
     3241 ACATCAGCAC CAGCACTGTG TCCACGCCTG TACCCCCGCC TGTCGATGAC ACCTGGCTCC 
     3301 AGAGCGCCAG CAGCCACAGC CCCACTCCAC AGCGCCGACC GGTGTCCAGC ATACACCCCC 
     3361 CTGGCCGGCC CCCAGCAGTG AGGGGCCCCA CTCCAGGGCC CCCCCTGATT CCTGTTCCCG 
     3421 TGGGGGCAGC AGCCTCCTTC TCGGCGCCCC CAATCCCATC CCGGCCTGGA CCCCAGAGCG 
     3481 TGTTTGCCAA CAGTGACCTC TTCCCAGCCC CGCCTCAGAT CCCATCTCGG CCAGTTCGGA 
     3541 TCCCCCCAGG GATTCCCCCA GGAGTGCCCA GCAGAAGACC CCCTGCTGCG CCCAGCCGGC 
     3601 CCACCATTAT CCGCCCAGCC GAGCCATCCC TGCTCGACAC GCGTACGCGG CCGCTCGAGC 
     3661 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3721 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3781 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3841 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3901 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3961 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4021 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4081 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4141 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4201 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4261 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4321 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4381 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4441 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4501 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4561 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4621 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4681 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4741 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4801 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4861 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4921 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4981 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5041 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5101 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5161 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5221 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5281 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5341 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5401 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5461 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5521 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5581 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5641 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5701 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5761 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5821 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5881 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5941 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6001 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6061 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6121 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6181 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6241 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6301 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6361 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6421 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6481 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6541 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6601 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6661 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6721 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6781 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6841 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6901 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6961 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7021 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7081 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7141 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7201 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7261 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7321 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7381 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7441 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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