LOCUS       RC223571L1                9132 bp    DNA     circular  SYN 
DEFINITION  GRIK3 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 3 (GRIK3)
ACCESSION   NM_000831
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7423..7603 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8331..8990 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7762..8330 
                     /label="ColE1"  
     misc_feature    6298..6318 
                     /label="LR50 primer"  
     misc_feature    6095..6166 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6307..6898 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6077..6083 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6077 
                     /label="GRIK3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GACCGCTCCC TGGCGGCGCC TCCGGAGTCT 
     3361 GGTTTGGGAA TACTGGGCCG GGCTCCTCGT GTGCGCCTTC TGGATCCCGG ACTCGCGCGG 
     3421 GATGCCCCAC GTCATCCGGA TCGGAGGAAT CTTCGAGTAT GCGGACGGCC CCAACGCCCA 
     3481 GGTCATGAAT GCCGAGGAGC ATGCCTTTCG ATTTTCTGCC AACATCATCA ACAGGAACAG 
     3541 GACTCTGCTG CCCAACACAA CCTTGACCTA TGACATACAG AGGATTCACT TCCATGACAG 
     3601 CTTCGAGGCG ACCAAAAAGG CCTGTGACCA GCTGGCACTG GGCGTGGTGG CGATCTTCGG 
     3661 CCCATCACAG GGCTCCTGCA CCAATGCCGT CCAGTCCATC TGCAATGCCC TGGAGGTGCC 
     3721 CCACATCCAG CTGCGTTGGA AGCACCACCC GCTGGACAAC AAGGACACCT TCTACGTGAA 
     3781 CCTCTACCCC GACTACGCCT CGCTCAGCCA TGCCATCCTC GACCTGGTCC AGTACCTCAA 
     3841 GTGGCGGTCA GCCACCGTGG TCTATGACGA CAGTACAGGG CTCATCCGAC TGCAGGAGCT 
     3901 CATCATGGCC CCATCAAGAT ACAACATCCG CCTGAAGATC CGTCAGCTCC CCATCGACTC 
     3961 TGACGACTCG CGCCCCTTGC TCAAGGAGAT GAAGCGAGGC CGGGAATTCC GCATTATCTT 
     4021 CGACTGCAGC CACACTATGG CGGCCCAGAT CCTCAAGCAG GCCATGGCCA TGGGCATGAT 
     4081 GACTGAGTAC TACCACTTCA TCTTCACCAC TCTGGATCTC TACGCTTTAG ACCTGGAGCC 
     4141 CTACCGCTAC TCAGGCGTGA ACCTGACAGG ATTCCGGATT CTCAATGTGG ACAACCCACA 
     4201 CGTCTCGGCC ATTGTGGAGA AGTGGTCCAT GGAGCGGCTG CAGGCAGCTC CCCGGTCCGA 
     4261 GTCTGGCCTG CTGGATGGAG TGATGATGAC TGATGCAGCC TTACTGTACG ACGCCGTCCA 
     4321 TATCGTGTCC GTGTGCTACC AGCGGGCACC ACAGATGACC GTGAACTCCC TGCAGTGCCA 
     4381 TCGGCACAAG GCCTGGCGCT TTGGCGGCCG CTTCATGAAC TTCATCAAGG AGGCTCAATG 
     4441 GGAAGGATTA ACTGGACGAA TTGTTTTCAA CAAAACTAGT GGCTTGCGGA CGGATTTTGA 
     4501 TCTGGACATC ATCAGCCTGA AAGAGGATGG CCTGGAGAAG GTTGGGGTGT GGAGTCCTGC 
     4561 CGACGGGCTC AACATCACTG AGGTTGCCAA AGGCCGAGGC CCTAATGTCA CCGACTCTCT 
     4621 GACAAACAGA TCACTCATTG TCACCACAGT GCTGGAGGAG CCCTTCGTCA TGTTTCGGAA 
     4681 ATCAGACAGG ACGCTATACG GGAATGACCG GTTCGAGGGC TACTGCATCG ACCTGCTAAA 
     4741 GGAGCTGGCC CACATCCTTG GTTTCTCCTA TGAGATCCGG CTGGTGGAGG ACGGCAAGTA 
     4801 CGGGGCACAG GATGACAAGG GCCAGTGGAA CGGCATGGTC AAGGAGCTCA TCGACCACAA 
     4861 GGCAGATCTG GCCGTGGCCC CCCTGACCAT CACCCATGTT CGAGAGAAGG CCATCGACTT 
     4921 CTCCAAGCCC TTCATGACAC TTGGTGTGAG CATCCTGTAT CGAAAGCCCA ATGGCACCAA 
     4981 CCCCAGCGTC TTCTCCTTCC TCAATCCCCT GTCCCCAGAC ATCTGGATGT ATGTTCTCCT 
     5041 CGCCTACCTG GGGGTCAGCT GTGTCCTCTT CGTCATCGCC AGGTTCAGCC CTTATGAGTG 
     5101 GTACGATGCT CACCCCTGCA ACCCTGGCTC CGAGGTGGTG GAAAATAACT TCACTCTGCT 
     5161 TAACAGCTTC TGGTTTGGAA TGGGATCCCT GATGCAGCAA GGGTCTGAGC TGATGCCCAA 
     5221 AGCCCTGTCC ACACGCATCA TTGGTGGCAT CTGGTGGTTC TTCACGCTCA TCATCATCTC 
     5281 TTCCTACACG GCCAACCTGG CTGCCTTTCT GACCGTGGAG CGCATGGAAT CACCCATTGA 
     5341 CTCTGCTGAT GACCTGGCCA AGCAAACCAA AATCGAGTAT GGGGCTGTCA AGGATGGGGC 
     5401 CACCATGACC TTCTTCAAGA AATCCAAGAT CTCCACCTTC GAGAAGATGT GGGCCTTCAT 
     5461 GAGCAGCAAG CCATCGGCGC TGGTGAAGAA CAACGAGGAG GGCATCCAGA GGGCCCTGAC 
     5521 GGCCGACTAC GCGCTGCTCA TGGAGTCCAC CACCATCGAG TACGTCACGC AGAGGAACTG 
     5581 CAACCTCACC CAGATCGGGG GCCTCATTGA CTCCAAGGGC TACGGCATCG GCACGCCCAT 
     5641 GGGCTCCCCA TACCGGGACA AGATCACCAT CGCCATCCTG CAGCTTCAGG AGGAGGACAA 
     5701 GCTGCATATC ATGAAGGAGA AGTGGTGGCG GGGCAGCGGG TGTCCTGAGG AGGAAAACAA 
     5761 AGAGGCCAGT GCCCTGGGGA TCCAGAAGAT CGGGGGCATC TTCATTGTCC TGGCCGCCGG 
     5821 GCTGGTCCTC TCTGTGCTGG TGGCCGTGGG CGAGTTTGTG TACAAGCTCC GCAAAACAGC 
     5881 AGAGAGAGAG CAGCGTTCCT TCTGCAGCAC CGTGGCCGAT GAGATCCGTT TCTCCCTTAC 
     5941 CTGCCAGCGT CGAGTCAAGC ACAAGCCTCA GCCTCCCATG ATGGTCAAGA CTGACGCCGT 
     6001 CATCAACATG CACACATTCA ATGACCGCCG GCTTCCCGGC AAGGACAGCA TGGCCTGCAG 
     6061 CACATCCTTA GCCCCTGTGT TCCCCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC 
     6121 AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA 
     6181 AACGGCCGGC CGCGGTCTGT ACAAGTAGGA TTCGTCGAGG GACCTAATAA CTTCGTATAG 
     6241 CATACATTAT ACGAAGTTAT ACATGTTTAA GGGTTCCGGT TCCACTAGGT ACAATTCGAT 
     6301 ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT 
     6361 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT 
     6421 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT 
     6481 CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT 
     6541 GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC 
     6601 GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG 
     6661 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC 
     6721 TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC 
     6781 GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG 
     6841 CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC 
     6901 CCGCATCGAT ACCGTCGACC TCGATCGAGA CCTAGAAAAA CATGGAGCAA TCACAAGTAG 
     6961 CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA GCACAAGAGG AGGAGGAGGT 
     7021 GGGTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA 
     7081 TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG 
     7141 ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC TACTTCCCTG ATTGGCAGAA 
     7201 CTACACACCA GGGCCAGGGA TCAGATATCC ACTGACCTTT GGATGGTGCT ACAAGCTAGT 
     7261 ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC CAATGAAGGA GAGAACACCC GCTTGTTACA 
     7321 CCCTGTGAGC CTGCATGGGA TGGATGACCC GGAGAGAGAA GTATTAGAGT GGAGGTTTGA 
     7381 CAGCCGCCTA GCATTTCATC ACATGGCCCG AGAGCTGCAT CCGGACTGTA CTGGGTCTCT 
     7441 CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA 
     7501 GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC 
     7561 TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGCATGTG 
     7621 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     7681 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     7741 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     7801 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     7861 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     7921 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     7981 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     8041 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     8101 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     8161 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     8221 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     8281 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAT 
     8341 TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA 
     8401 TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG 
     8461 CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC 
     8521 ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC 
     8581 TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA 
     8641 TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT 
     8701 TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA 
     8761 CCGTCTTTCA TTGCCATACG GAACTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA 
     8821 ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 
     8881 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT 
     8941 TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATA 
     9001 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     9061 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTCC CGCGCACATT TCCCCGAAAA 
     9121 GTGCCACCTG AC
//