LOCUS       RC223571                7638 bp    DNA     circular  SYN 
DEFINITION  GRIK3 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 3 (GRIK3)
ACCESSION   NM_000831
VERSION     NM_000831.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3795..3886 
                     /label="Myc-DDK"  
     misc_feature    3916..4501 
                     /label="PolyA signal"  
     misc_feature    3973..3992 
                     /label="Restriction Enzyme"  
     misc_feature    4777..5449 
                     /label="ColE1"  
     misc_feature    5879..6673 
                     /label="Kan/Neo"  
     misc_feature    6708..7010 
                     /label="SV40 ori"  
     misc_feature    7096..7573 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3777..3783 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3777 
                     /label="GRIK3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGCTCCC TGGCGGCGCC TCCGGAGTCT GGTTTGGGAA TACTGGGCCG 
     1081 GGCTCCTCGT GTGCGCCTTC TGGATCCCGG ACTCGCGCGG GATGCCCCAC GTCATCCGGA 
     1141 TCGGAGGAAT CTTCGAGTAT GCGGACGGCC CCAACGCCCA GGTCATGAAT GCCGAGGAGC 
     1201 ATGCCTTTCG ATTTTCTGCC AACATCATCA ACAGGAACAG GACTCTGCTG CCCAACACAA 
     1261 CCTTGACCTA TGACATACAG AGGATTCACT TCCATGACAG CTTCGAGGCG ACCAAAAAGG 
     1321 CCTGTGACCA GCTGGCACTG GGCGTGGTGG CGATCTTCGG CCCATCACAG GGCTCCTGCA 
     1381 CCAATGCCGT CCAGTCCATC TGCAATGCCC TGGAGGTGCC CCACATCCAG CTGCGTTGGA 
     1441 AGCACCACCC GCTGGACAAC AAGGACACCT TCTACGTGAA CCTCTACCCC GACTACGCCT 
     1501 CGCTCAGCCA TGCCATCCTC GACCTGGTCC AGTACCTCAA GTGGCGGTCA GCCACCGTGG 
     1561 TCTATGACGA CAGTACAGGG CTCATCCGAC TGCAGGAGCT CATCATGGCC CCATCAAGAT 
     1621 ACAACATCCG CCTGAAGATC CGTCAGCTCC CCATCGACTC TGACGACTCG CGCCCCTTGC 
     1681 TCAAGGAGAT GAAGCGAGGC CGGGAATTCC GCATTATCTT CGACTGCAGC CACACTATGG 
     1741 CGGCCCAGAT CCTCAAGCAG GCCATGGCCA TGGGCATGAT GACTGAGTAC TACCACTTCA 
     1801 TCTTCACCAC TCTGGATCTC TACGCTTTAG ACCTGGAGCC CTACCGCTAC TCAGGCGTGA 
     1861 ACCTGACAGG ATTCCGGATT CTCAATGTGG ACAACCCACA CGTCTCGGCC ATTGTGGAGA 
     1921 AGTGGTCCAT GGAGCGGCTG CAGGCAGCTC CCCGGTCCGA GTCTGGCCTG CTGGATGGAG 
     1981 TGATGATGAC TGATGCAGCC TTACTGTACG ACGCCGTCCA TATCGTGTCC GTGTGCTACC 
     2041 AGCGGGCACC ACAGATGACC GTGAACTCCC TGCAGTGCCA TCGGCACAAG GCCTGGCGCT 
     2101 TTGGCGGCCG CTTCATGAAC TTCATCAAGG AGGCTCAATG GGAAGGATTA ACTGGACGAA 
     2161 TTGTTTTCAA CAAAACTAGT GGCTTGCGGA CGGATTTTGA TCTGGACATC ATCAGCCTGA 
     2221 AAGAGGATGG CCTGGAGAAG GTTGGGGTGT GGAGTCCTGC CGACGGGCTC AACATCACTG 
     2281 AGGTTGCCAA AGGCCGAGGC CCTAATGTCA CCGACTCTCT GACAAACAGA TCACTCATTG 
     2341 TCACCACAGT GCTGGAGGAG CCCTTCGTCA TGTTTCGGAA ATCAGACAGG ACGCTATACG 
     2401 GGAATGACCG GTTCGAGGGC TACTGCATCG ACCTGCTAAA GGAGCTGGCC CACATCCTTG 
     2461 GTTTCTCCTA TGAGATCCGG CTGGTGGAGG ACGGCAAGTA CGGGGCACAG GATGACAAGG 
     2521 GCCAGTGGAA CGGCATGGTC AAGGAGCTCA TCGACCACAA GGCAGATCTG GCCGTGGCCC 
     2581 CCCTGACCAT CACCCATGTT CGAGAGAAGG CCATCGACTT CTCCAAGCCC TTCATGACAC 
     2641 TTGGTGTGAG CATCCTGTAT CGAAAGCCCA ATGGCACCAA CCCCAGCGTC TTCTCCTTCC 
     2701 TCAATCCCCT GTCCCCAGAC ATCTGGATGT ATGTTCTCCT CGCCTACCTG GGGGTCAGCT 
     2761 GTGTCCTCTT CGTCATCGCC AGGTTCAGCC CTTATGAGTG GTACGATGCT CACCCCTGCA 
     2821 ACCCTGGCTC CGAGGTGGTG GAAAATAACT TCACTCTGCT TAACAGCTTC TGGTTTGGAA 
     2881 TGGGATCCCT GATGCAGCAA GGGTCTGAGC TGATGCCCAA AGCCCTGTCC ACACGCATCA 
     2941 TTGGTGGCAT CTGGTGGTTC TTCACGCTCA TCATCATCTC TTCCTACACG GCCAACCTGG 
     3001 CTGCCTTTCT GACCGTGGAG CGCATGGAAT CACCCATTGA CTCTGCTGAT GACCTGGCCA 
     3061 AGCAAACCAA AATCGAGTAT GGGGCTGTCA AGGATGGGGC CACCATGACC TTCTTCAAGA 
     3121 AATCCAAGAT CTCCACCTTC GAGAAGATGT GGGCCTTCAT GAGCAGCAAG CCATCGGCGC 
     3181 TGGTGAAGAA CAACGAGGAG GGCATCCAGA GGGCCCTGAC GGCCGACTAC GCGCTGCTCA 
     3241 TGGAGTCCAC CACCATCGAG TACGTCACGC AGAGGAACTG CAACCTCACC CAGATCGGGG 
     3301 GCCTCATTGA CTCCAAGGGC TACGGCATCG GCACGCCCAT GGGCTCCCCA TACCGGGACA 
     3361 AGATCACCAT CGCCATCCTG CAGCTTCAGG AGGAGGACAA GCTGCATATC ATGAAGGAGA 
     3421 AGTGGTGGCG GGGCAGCGGG TGTCCTGAGG AGGAAAACAA AGAGGCCAGT GCCCTGGGGA 
     3481 TCCAGAAGAT CGGGGGCATC TTCATTGTCC TGGCCGCCGG GCTGGTCCTC TCTGTGCTGG 
     3541 TGGCCGTGGG CGAGTTTGTG TACAAGCTCC GCAAAACAGC AGAGAGAGAG CAGCGTTCCT 
     3601 TCTGCAGCAC CGTGGCCGAT GAGATCCGTT TCTCCCTTAC CTGCCAGCGT CGAGTCAAGC 
     3661 ACAAGCCTCA GCCTCCCATG ATGGTCAAGA CTGACGCCGT CATCAACATG CACACATTCA 
     3721 ATGACCGCCG GCTTCCCGGC AAGGACAGCA TGGCCTGCAG CACATCCTTA GCCCCTGTGT 
     3781 TCCCCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3841 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3901 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3961 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4021 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4081 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4141 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4201 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4261 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4321 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4381 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4441 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4501 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4561 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4621 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4681 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4741 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4801 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4861 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4921 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4981 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5041 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5101 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5161 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5221 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5281 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5341 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5401 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5461 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5521 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5581 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5641 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5701 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5761 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5821 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5881 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5941 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6001 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6061 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6121 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6181 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6241 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6301 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6361 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6421 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6481 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6541 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6601 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6661 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6721 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6781 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6841 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6901 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6961 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7021 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7081 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7141 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7201 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7261 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7321 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7381 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7441 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7501 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7561 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7621 AAATTTAACG CGAATTTT
//