LOCUS       RC223440                8367 bp    DNA     circular  SYN 
DEFINITION  KDM2A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 2A (KDM2A), transcript variant 1
ACCESSION   NM_012308
VERSION     NM_012308.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4524..4615 
                     /label="Myc-DDK"  
     misc_feature    4645..5230 
                     /label="PolyA signal"  
     misc_feature    4702..4721 
                     /label="Restriction Enzyme"  
     misc_feature    5506..6178 
                     /label="ColE1"  
     misc_feature    6608..7402 
                     /label="Kan/Neo"  
     misc_feature    7437..7739 
                     /label="SV40 ori"  
     misc_feature    7825..8302 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4506..4512 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4506 
                     /label="KDM2A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACCCGAA GAAGAAAGGA TTCGTTACAG CCAGAGATTG CGTGGTACCA 
     1081 TGCGACGACG CTATGAAGAT GATGGCATTT CAGATGATGA AATTGAAGGA AAAAGAACTT 
     1141 TTGACTTGGA AGAGAAACTG CACACCAACA AATATAATGC CAATTTTGTT ACTTTTATGG 
     1201 AAGGAAAAGA TTTTAATGTA GAGTATATTC AGCGGGGTGG CTTGAGAGAT CCTCTGATTT 
     1261 TCAAGAATTC TGATGGACTC GGAATAAAAA TGCCGGATCC AGACTTCACT GTGAATGATG 
     1321 TCAAAATGTG TGTGGGGAGT CGTCGCATGG TGGATGTCAT GGACGTGAAC ACACAGAAAG 
     1381 GCATTGAAAT GACCATGGCT CAGTGGACAC GCTACTATGA GACCCCAGAG GAGGAGCGAG 
     1441 AGAAACTCTA TAATGTCATC AGCCTCGAGT TTAGCCACAC CAGGCTGGAG AATATGGTGC 
     1501 AGAGGCCCTC CACGGTGGAT TTCATTGACT GGGTAGACAA CATGTGGCCA AGGCACTTGA 
     1561 AGGAAAGCCA GACTGAATCA ACAAATGCCA TCTTGGAGAT GCAGTACCCT AAAGTGCAGA 
     1621 AGTACTGTCT AATGAGTGTT CGAGGCTGCT ATACTGACTT CCATGTGGAC TTTGGTGGTA 
     1681 CCTCTGTTTG GTATCACATC CATCAAGGGG GAAAGGTCTT CTGGCTCATC CCCCCTACAG 
     1741 CCCACAACCT GGAGCTGTAC GAGAATTGGC TGCTGTCAGG GAAACAGGGA GACATCTTTC 
     1801 TGGGTGACCG GGTATCAGAT TGTCAGCGCA TTGAGCTCAA GCAGGGCTAT ACCTTCGTCA 
     1861 TTCCCTCAGG CTGGATTCAT GCTGTGTATA CTCCTACAGA CACATTAGTG TTTGGGGGCA 
     1921 ATTTTTTGCA TAGCTTCAAC ATCCCTATGC AGTTAAAAAT ATACAACATT GAAGATCGGA 
     1981 CACGGGTTCC AAATAAGTTT CGCTATCCAT TCTACTATGA GATGTGTTGG TATGTGTTGG 
     2041 AGCGCTATGT GTACTGCATA ACCAACCGTT CCCACCTAAC CAAGGAATTT CAGAAAGAGT 
     2101 CCCTCAGCAT GGATTTGGAG TTAAATGGGT TGGAGTCTGG GAATGGGGAT GAGGAAGCAG 
     2161 TGGATCGAGA ACCCCGACGC TTGAGCAGCA GGCGTTCTGT CCTCACTAGC CCTGTAGCGA 
     2221 ATGGAGTCAA CCTGGATTAT GATGGACTGG GCAAAACCTG CCGAAGTCTT CCAAGTCTGA 
     2281 AGAAAACTTT GGCTGGGGAC TCATCTTCTG ACTGTAGCCG GGGCTCCCAC AATGGACAAG 
     2341 TGTGGGATCC CCAGTGTGCT CCCCGAAAGG ACAGGCAAGT GCATCTGACC CATTTTGAGC 
     2401 TTGAAGGCCT TCGCTGCCTT GTAGATAAGT TGGAGTCTCT GCCACTGCAC AAGAAATGTG 
     2461 TCCCCACAGG GATAGAAGAT GAAGATGCTC TCATTGCTGA TGTAAAGATT TTGCTGGAGG 
     2521 AGCTTGCCAA CAGCGATCCC AAGTTAGCCC TCACTGGAGT TCCTATAGTA CAGTGGCCAA 
     2581 AAAGGGATAA GCTTAAATTC CCCACTCGGC CAAAGGTGCG GGTTCCTACC ATCCCCATTA 
     2641 CGAAGCCTCA CACTATGAAA CCAGCTCCAC GGTTAACACC TGTGAGGCCA GCTGCTGCCT 
     2701 CCCCGATTGT GTCAGGAGCC AGACGGAGAC GAGTGCGATG TCGAAAATGC AAAGCCTGTG 
     2761 TGCAAGGAGA GTGTGGTGTT TGCCACTACT GCAGAGACAT GAAGAAGTTT GGGGGGCCTG 
     2821 GACGCATGAA GCAGTCCTGT GTCCTCCGAC AGTGCTTGGC ACCCAGACTG CCTCACTCAG 
     2881 TCACATGTTC CCTCTGTGGA GAGGTGGATC AGAATGAAGA GACACAAGAC TTTGAGAAGA 
     2941 AACTCATGGA ATGCTGTATC TGCAATGAGA TTGTTCATCC TGGCTGCCTC CAGATGGACG 
     3001 GAGAGGGGTT GCTTAACGAA GAATTGCCAA ATTGCTGGGA ATGTCCAAAG TGCTACCAGG 
     3061 AGGACAGCTC GGAGAAAGCC CAGAAGCGGA AAATGGAAGA GAGTGACGAA GAAGCTGTGC 
     3121 AAGCCAAAGT CCTGCGGCCC CTGCGGAGCT GCGATGAGCC TCTCACGCCC CCGCCTCATT 
     3181 CACCCACTTC CATGCTGCAG CTCATCCATG ACCCGGTTTC CCCCCGGGGT ATGGTGACTC 
     3241 GGTCATCCCC TGGGGCTGGC CCCAGCGACC ACCACAGTGC CAGCCGCGAT GAGCGCTTCA 
     3301 AACGGCGGCA GTTGCTGCGG CTGCAGGCCA CAGAGCGCAC CATGGTACGG GAAAAGGAGA 
     3361 ACAATCCCAG CGGCAAAAAG GAGCTGTCTG AAGTTGAGAA AGCCAAGATC CGGGGATCGT 
     3421 ACCTCACTGT CACGCTACAG AGGCCCACCA AAGAGCTCCA CGGGACATCC ATTGTGCCCA 
     3481 AGCTGCAGGC CATCACGGCC TCCTCTGCCA ACCTTCGCCA TTCCCCCCGT GTGCTAGTGC 
     3541 AGCACTGCCC AGCCCGAACC CCCCAGCGTG GGGATGAGGA GGGGCTGGGG GGAGAGGAGG 
     3601 AGGAAGAGGA GGAGGAGGAG GAGGAAGATG ACAGTGCAGA GGAGGGGGGT GCAGCCAGGC 
     3661 TGAATGGCCG GGGCAGTTGG GCTCAGGATG GAGACGAAAG CTGGATGCAG CGGGAGGTCT 
     3721 GGATGTCTGT CTTCCGCTAC CTCAGCCGCA GAGAACTTTG TGAATGTATG CGAGTGTGCA 
     3781 AGACGTGGTA TAAATGGTGC TGCGACAAGA GACTTTGGAC AAAAATTGAC TTGAGTAGGT 
     3841 GTAAGGCCAT TGTGCCCCAG GCCCTCAGTG GCATCATCAA GAGGCAGCCA GTCAGCCTTG 
     3901 ACCTCAGTTG GACCAACATC TCTAAAAAGC AACTGACATG GCTCGTCAAT AGGCTGCCAG 
     3961 GACTGAAAGA CCTCCTCCTA GCAGGCTGCT CCTGGTCTGC AGTCTCTGCC CTCAGCACCT 
     4021 CCAGCTGCCC CCTTCTCAGG ACCCTTGATC TTCGGTGGGC AGTAGGAATC AAGGACCCTC 
     4081 AAATTCGGGA CTTGCTTACT CCACCGGCTG ATAAACCAGG TCAGGACAAT CGCAGCAAGC 
     4141 TCCGGAACAT GACCGACTTC CGGCTGGCAG GCCTTGACAT CACAGATGCC ACGCTTCGCC 
     4201 TCATAATTCG CCACATGCCC CTCCTGTCTC GACTCGACCT CAGTCACTGC AGCCACCTTA 
     4261 CAGATCAGTC CTCCAATCTA CTCACTGCTG TCGGGTCTTC CACTCGCTAC TCTCTCACAG 
     4321 AGCTCAATAT GGCAGGTTGC AATAAATTGA CAGACCAGAC CCTGATCTAC CTACGGCGCA 
     4381 TTGCCAACGT CACCTTGATC GACCTTCGAG GATGCAAGCA GATCACTCGA AAAGCCTGCG 
     4441 AGCACTTCAT CTCAGACTTG TCCATCAACA GCCTCTACTG CCTGTCTGAC GAGAAGCTGA 
     4501 TACAGAAGAT CAGCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4561 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4621 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4681 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4741 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4801 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4861 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4921 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4981 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5041 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5101 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5161 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5221 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5281 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5341 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5401 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5461 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5521 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5581 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5641 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5701 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5761 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5821 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5881 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5941 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6001 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6061 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6121 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6181 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6241 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6301 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6361 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6421 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6481 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6541 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6601 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6661 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6721 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6781 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6841 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6901 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6961 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7021 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7081 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7141 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7201 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7261 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7321 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7381 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7441 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7501 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7561 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7621 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7681 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7801 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7861 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7921 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7981 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8041 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8101 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8161 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8221 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8281 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8341 ATTTAACAAA AATTTAACGC GAATTTT
//